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| Registros recuperados : 12 | |
| 1. |  | NUNES, E.; FRIHLING, B.; BARROS, E.; OLIVEIRA, C. de; VERBISCK, N. V.; FLORES, T.; FREITAS JUNIOR, A. de; FRANCO, O.; MACEDO, M. de; MIGLIOLO, L.; LUNA, K. Antibiofilm Activity of Acidic Phospholipase Isoform Isolated from Bothrops erythromelas Snake Venom. Toxins, 2020.| Biblioteca(s): Embrapa Gado de Corte. |
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| 2. | | OLIVEIRA, A. S.; MIGLIOLO, L.; AQUINO, R. O.; RIBEIRO, J. K. C.; MACEDO, L. L. P.; BEMQUERER, M. P.; SANTOS, E. A.; KIYOTA, S.; SALES, M. P. de. Two Kunitz-type inhibitors with activity against trypsin and papain from Pithcellobium dumosum seeds: purification, characterization, and activiy towards pest insect digestive enzyme. Protein & Peptide Letter, v. 16, p. 1526-1532, 2009.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 3. | | MACHADO, R. J. A.; MONTEIRO, N. K. V.; MIGLIOLO, L.; SILVA, O. N.; PINTO, M. F. S.; OLIVEIRA, A. S.; FRANCO, O. L.; KIYOTA, S.; BEMQUERER, M. P.; UCHOA, A. F.; MORAIS, A. H. A.; SANTOS, E. A. Characterization and pharmacological properties of a novel multifunctional Kunitz inhibitor from Erythrina velutina seeds. Plos One, v. 8, n. 5, e63571, 2013.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 4. | | VIANA, J. F. C.; CARRIJO, J.; FREITAS, C. G.; PAUL, A.; ALCARAZ, J.; LACORTE, C. C.; MIGLIOLO, L.; ANDRADE, C. A.; FALCAO, R.; SANTOS, N. C.; GONÇALVES, S.; OTERO-GONZÁLES, A. J.; KHADEMHOSSEINI, A.; DIAS, S. C.; FRANCO, O. L. Antifungal nanofibers made by controlled release of sea animal derived peptide. Nanoscale, v. 7, p. 6238-6246, 2015.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 5. | | AMARAL, A. C.; SILVA, O. N.; MUNDIM, N. C. C. R.; CARVALHO, M. J. A. de; MIGLIOLO, L.; LEITE, J. R. S. A.; PRATES, M. V.; BOCCA, A. L.; FRANCO, O. C.; FELIPE, M. S. S. Predicting antimicrobial peptides from eukaryotic genomes: In silico strategies to develop antibiotics. Peptides, v. 37, p. 301-308, 2012.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | OLIVEIRA, A. S.; PITANGA, J. C. M.; RIBEIRO, J. K. C.; MACEDO, L. L. P.; MIGLIOLO, L.; AQUINO, R. O.; COELHO, F. P.; SANCHES, A. P. C.; BEMQUERER, M. P.; SANTOS, E. A.; KIYOTA, S; SALES, M. P. Purification and characterization of a bifuncional kunitz type inhibitor from Pithecolobium dumosum and its effect in vitro. On insect pests. In: ANNUAL MEETING OF THE SBBq, 36.; IUBMB CONFERENCE, 10., 2007, Salvador, BA. Infectious diseases: biochemistry of parasites, vectors and hosts: program and abstracts. São Paulo, SP: Brazilian Society for Biochemistry and Molecular Biology, 2007.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | FRIHLING, B. E. F.; BOLETI, A. P. de A.; OLIVEIRA, C. F. R. de; SANCHES, S. C.; CARDOSO, P. H. de O.; VERBISCK, N. V.; MACEDO, M. L. R.; SANTA RITA, P. H.; CARVALHO, C. M. E.; MIGLIOLO, L. Purification, characterization and evaluation of the antitumoral activity of a phospholipase A2 from the snake Bothrops moojeni. Pharmaceuticals, v. 15, n. 6, article 724, 2022. Na publicação: Newton Verbisck.| Biblioteca(s): Embrapa Gado de Corte. |
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| 8. | | OLIVEIRA, A. S.; MIGLIOLO, L.; AQUINO, R. O.; RIBEIRO, J. K. C.; MACEDO, L. L. P.; COELHO, F. S.; SANCHES, A. P. C.; BEMQUERER, M. P.; SANTOS, E. A.; KIYOTA, S. SALES, M. P. Purification of a kunitz-type inhibitor from Pithecolobium dumosum seeds and its effect towards insects. In: ANNUAL MEETING OF THE SBBq, 36.; IUBMB CONFERENCE, 10., 2007, Salvador, BA. Infectious diseases: biochemistry of parasites, vectors and hosts: program and abstracts. São Paulo, SP: Brazilian Society for Biochemistry and Molecular Biology, 2007.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | OLIVEIRA, A. S.; AQUINO, R. O.; MIGLIOLO, L.; RIBEIRO, J. K. C.; MACEDO, L. L. P.; COELHO, F. S.; FOOK, J. M. L. L.; BEMQUERER, M. P.; SANTOS, E. A.; KIYOTA, S.; SALES, M. P. Two isoforms of kunitz-type inhibitors from Pithecolobium dumosum and their in vitro activities against insect larvae. In: ANNUAL MEETING OF THE SBBq, 36.; IUBMB CONFERENCE, 10., 2007, Salvador, BA. Infectious diseases: biochemistry of parasites, vectors and hosts: program and abstracts. São Paulo, SP: Brazilian Society for Biochemistry and Molecular Biology, 2007.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | CRUZ, A. C. B.; MASSENA, F. S.; MIGLIOLO, L.; MACEDO, L. L. P.; MONTEIRO, N. K. V.; OLIVEIRA, A. S.; MACEDO, F. P.; UCHOA, A. F.; SA, M. F. G. de; VASCONCELOS, I. M.; MURAD, A. M.; FRANCO, O. L.; SANTOS, E. A. Bioinsecticidal activity of a novel Kunitz trypsin inhibitor from Catanduva (Piptadenia moniliformis) seeds. Plant Physiology and Biochemistry, v. 70, p. 61-68, 2013.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | MACHADO, R. J. A.; MONTEIRO, N. K. V.; SERQUIZ, P. R.; CARVALHO, F. M. C.; BEZERRA, A. D. L.; MIGLIOLO, L.; SILVA, O. N.; PINTO, M. S.; OLIVEIRA, A. S.; FRANCO, O. L.; KIYOTA, S.; BEMQUERER, M. P.; MORAIS, A. H. A.; SANTOS, E. A. Anticoagulant and anti-inflammatory activities of a trypsin inhibitor present in Erythrina velutina seeds. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUÍMICS E BIOLOGIA MOLECULAR - SBBq, 41., Foz do Iguaçú, Brasil. Resumos... Foz do Iguaçu: SBBq, 2012.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | ARCANJO, D. D. R.; VASCONCELOS, A. G.; COMERMA-STEFFENSEN, S. G.; JESUS, J. R.; SILVA, L. P. da; PIRES JÚNIOR, O. R.; COSTA-NETO, C. M.; OLIVEIRA, E. B.; MIGLIOLO, L.; FRANCO, O. L.; RESTINI, C. B. A.; PAULO, M.; BENDHACK, L. M.; BEMQUERER, M. P.; OLIVEIRA, A. P.; SIMONSEN, U.; LEITE, J. R. de S. de A. A novel vasoactive proline-rich oligopeptide from the skin secretion of the frog Brachycephalus ephippium. Plos One, v. 10, dez. 2015. (Open Access)| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 12 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
06/01/2023 |
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Data da última atualização: |
06/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
FRIHLING, B. E. F.; BOLETI, A. P. de A.; OLIVEIRA, C. F. R. de; SANCHES, S. C.; CARDOSO, P. H. de O.; VERBISCK, N. V.; MACEDO, M. L. R.; SANTA RITA, P. H.; CARVALHO, C. M. E.; MIGLIOLO, L. |
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Afiliação: |
BRENO EMANUEL FARIAS FRIHLING, UNIVERSIDADE CATÓLICA DOM BOSCO; ANA PAULA DE ARAÚJO BOLETI, UNIVERSIDADE CATÓLICA DOM BOSCO; CAIO FERNANDO RAMALHO DE OLIVEIRA, UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL; SIMONE CAMARGO SANCHES, UNIVERSIDADE CATÓLICA DOM BOSCO; PEDRO HENRIQUE DE OLIVEIRA CARDOSO, UNIVERSIDADE CATÓLICA DOM BOSCO; NEWTON VALERIO VERBISCK, CNPGC; MARIA LÍGIA RODRIGUES MACEDO, UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL; PAULA HELENA SANTA RITA, UNIVERSIDADE CATÓLICA DOM BOSCO; CRISTIANO MARCELO ESPINOLA CARVALHO, UNIVERSIDADE CATÓLICA DOM BOSCO; LUDOVICO MIGLIOLO, UNIVERSIDADE FEDERAL DO RIO GRANDE DO NORTE. |
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Título: |
Purification, characterization and evaluation of the antitumoral activity of a phospholipase A2 from the snake Bothrops moojeni. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Pharmaceuticals, v. 15, n. 6, article 724, 2022. |
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DOI: |
https://doi.org/10.3390/ph15060724 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Newton Verbisck. |
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Conteúdo: |
Nature presents a wide range of biomolecules with pharmacological potential, including venomous animal proteins. Among the protein components from snake venoms, phospholipases (PLA2) are of great importance for the development of new anticancer compounds. Thus, we aimed to evaluate the PLA2 anticancer properties from Bothrops moojeni venom. The crude venom was purified through three chromatographic steps, monitored by enzymatic activity and SDS-PAGE (12%). The purified PLA2 denominated BmPLA2 had its molecular mass and N-terminal sequence identified by mass spectrometry and Edman degradation, respectively. BmPLA2 was assayed against human epithelial colorectal adenocarcinoma cells (Caco-2), human rhabdomyosarcoma cells (RD) and mucoepidermoid carcinoma of the lung (NCI-H292), using human fibroblast cells (MRC-5) and microglia cells (BV-2) as a cytotoxicity control. BmPLA2 presented 13,836 Da and a 24 amino acid-residue homologue with snake PLA2, which showed a 90% similarity with other Bothrops moojeni PLA2. BmPLA2 displayed an IC50 of 0.6 M against Caco-2, and demonstrated a selectivity index of 1.85 (compared to MRC-5) and 6.33 (compared to BV-2), supporting its selectivity for cancer cells. In conclusion, we describe a new acidic phospholipase, which showed antitumor activity and is a potential candidate in the development of new biotechnological tools. |
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Thesagro: |
Biotecnologia; Espectrometria; Proteína. |
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Thesaurus NAL: |
Animal proteins; Biotechnology; Bothrops; Cell membranes; Mass spectrometry; Phospholipase A2. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1150743/1/Purification-characterization-evaluation-2022.pdf
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Marc: |
LEADER 02489naa a2200361 a 4500
001 2150743
005 2023-01-06
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/ph15060724$2DOI
100 1 $aFRIHLING, B. E. F.
245 $aPurification, characterization and evaluation of the antitumoral activity of a phospholipase A2 from the snake Bothrops moojeni.$h[electronic resource]
260 $c2022
500 $aNa publicação: Newton Verbisck.
520 $aNature presents a wide range of biomolecules with pharmacological potential, including venomous animal proteins. Among the protein components from snake venoms, phospholipases (PLA2) are of great importance for the development of new anticancer compounds. Thus, we aimed to evaluate the PLA2 anticancer properties from Bothrops moojeni venom. The crude venom was purified through three chromatographic steps, monitored by enzymatic activity and SDS-PAGE (12%). The purified PLA2 denominated BmPLA2 had its molecular mass and N-terminal sequence identified by mass spectrometry and Edman degradation, respectively. BmPLA2 was assayed against human epithelial colorectal adenocarcinoma cells (Caco-2), human rhabdomyosarcoma cells (RD) and mucoepidermoid carcinoma of the lung (NCI-H292), using human fibroblast cells (MRC-5) and microglia cells (BV-2) as a cytotoxicity control. BmPLA2 presented 13,836 Da and a 24 amino acid-residue homologue with snake PLA2, which showed a 90% similarity with other Bothrops moojeni PLA2. BmPLA2 displayed an IC50 of 0.6 M against Caco-2, and demonstrated a selectivity index of 1.85 (compared to MRC-5) and 6.33 (compared to BV-2), supporting its selectivity for cancer cells. In conclusion, we describe a new acidic phospholipase, which showed antitumor activity and is a potential candidate in the development of new biotechnological tools.
650 $aAnimal proteins
650 $aBiotechnology
650 $aBothrops
650 $aCell membranes
650 $aMass spectrometry
650 $aPhospholipase A2
650 $aBiotecnologia
650 $aEspectrometria
650 $aProteína
700 1 $aBOLETI, A. P. de A.
700 1 $aOLIVEIRA, C. F. R. de
700 1 $aSANCHES, S. C.
700 1 $aCARDOSO, P. H. de O.
700 1 $aVERBISCK, N. V.
700 1 $aMACEDO, M. L. R.
700 1 $aSANTA RITA, P. H.
700 1 $aCARVALHO, C. M. E.
700 1 $aMIGLIOLO, L.
773 $tPharmaceuticals$gv. 15, n. 6, article 724, 2022.
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