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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
15/02/2016 |
Data da última atualização: |
17/02/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BLECHA, I. M. Z.; SIQUEIRA, F.; FERREIRA, A. B. R.; FEIJO, G. L. D.; TORRES JUNIOR, R. A. de A.; MEDEIROS, S. R. de. |
Afiliação: |
I.M.Z. BLECHA, Universidade Estadual de Mato Grosso do Sul, Aquidauana, MS, Brasil; FABIANE SIQUEIRA, CNPGC; ANNA BEATRIZ ROBOTTON FERREIRA, CNPGC; GELSON LUIS DIAS FEIJO, CNPGC; ROBERTO AUGUSTO DE A TORRES JUNIOR, CNPGC; SERGIO RAPOSO DE MEDEIROS, CNPGC. |
Título: |
Identification and evaluation of polymorphisms in FABP3 and FABP4 in beef cattle. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Genetics and Molecular Research, v.14, n. 4, p. 16353-16363, 2015 |
Idioma: |
Português |
Palavras-Chave: |
A-FABP; Back fat; H-FABP; Molecular marker; Ribeye area. |
Thesaurus Nal: |
marbling. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/139036/1/Blecha-da-Fabiane-2015-gmr5390.pdf
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Marc: |
LEADER 00710naa a2200241 a 4500 001 2037002 005 2016-02-17 008 2015 bl uuuu u00u1 u #d 100 1 $aBLECHA, I. M. Z. 245 $aIdentification and evaluation of polymorphisms in FABP3 and FABP4 in beef cattle.$h[electronic resource] 260 $c2015 650 $amarbling 653 $aA-FABP 653 $aBack fat 653 $aH-FABP 653 $aMolecular marker 653 $aRibeye area 700 1 $aSIQUEIRA, F. 700 1 $aFERREIRA, A. B. R. 700 1 $aFEIJO, G. L. D. 700 1 $aTORRES JUNIOR, R. A. de A. 700 1 $aMEDEIROS, S. R. de 773 $tGenetics and Molecular Research$gv.14, n. 4, p. 16353-16363, 2015
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Embrapa Gado de Corte (CNPGC) |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
10/02/2010 |
Data da última atualização: |
03/08/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
MENNA, P.; BARCELLOS, F. G.; HUNGRIA, M. |
Afiliação: |
PÂMELA MENNA, CNPSo/UEL/CNPq-MCT; FERNANDO GOMES BARCELLOS, CNPSo/CNPq-MCT; MARIANGELA HUNGRIA DA CUNHA, CNPSo. |
Título: |
Phylogeny and taxonomy of a diverse collection of Bradyrhizobium strains based on multilocus sequence analysis of the 16S rRNA gene, ITS region and glnll, recA, atpD and dnaK genes. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
International Journal of Systematic and Evolutionary Microbiology, Great Britain, v. 59, n. 12, p. 2934-2950, dez. 2009. |
DOI: |
10.1099/ijs.0.009779-0 |
Idioma: |
Inglês |
Conteúdo: |
The genus Bradyrhizobium encompasses a variety of bacteria that can live in symbiotic and endophytic associations with legumes and non-legumes, and are characterized by physiological and symbiotic versatility and broad geographical distribution. However, despite indications of great genetic variability within the genus, only eight species have been described, mainly because of the highly conserved nature of the 16S rRNA gene. In this study, 169 strains isolated from 43 different legumes were analysed by rep-PCR with the BOX primer, by sequence analysis of the 16S rRNA gene and the 16S?23S rRNA intergenic transcribed spacer (ITS) and by multilocus sequence analysis (MLSA) of four housekeeping genes, glnII, recA, atpD and dnaK. Considering a cut-off at a level of 70 % similarity, 80 rep-PCR profiles were distinguished, which, together with type strains, were clustered at a very low level of similarity (24 %). In both single and concatenated analyses of the 16S rRNA gene and ITS sequences, two large groups were formed, with bootstrap support of 99 % in the concatenated analysis. The first group included the type and/or reference strains of Bradyrhizobium japonicum, B. betae, B. liaoningense, B. canariense and B. yuanmingense and B. japonicum USDA 110, and the second group included strains related to Bradyrhizobium elkanii USDA 76T, B. pachyrhizi PAC48T and B. jicamae PAC68T. Similar results were obtained with MLSA of glnII, recA, atpD and dnaK. Greatest variability was observed when the atpD gene was amplified, and five strains related to B. elkanii revealed a level of variability never reported before. Another important observation was that a group composed of strains USDA 110, SEMIA 5080 and SEMIA 6059, all isolated from soybean, clustered in all six trees with high bootstrap support and were quite distinct from the clusters that included B. japonicum USDA 6T. The results confirm that MLSA is a rapid and reliable way of providing information on phylogenetic relationships and of identifying rhizobial strains potentially representative of novel species. MenosThe genus Bradyrhizobium encompasses a variety of bacteria that can live in symbiotic and endophytic associations with legumes and non-legumes, and are characterized by physiological and symbiotic versatility and broad geographical distribution. However, despite indications of great genetic variability within the genus, only eight species have been described, mainly because of the highly conserved nature of the 16S rRNA gene. In this study, 169 strains isolated from 43 different legumes were analysed by rep-PCR with the BOX primer, by sequence analysis of the 16S rRNA gene and the 16S?23S rRNA intergenic transcribed spacer (ITS) and by multilocus sequence analysis (MLSA) of four housekeeping genes, glnII, recA, atpD and dnaK. Considering a cut-off at a level of 70 % similarity, 80 rep-PCR profiles were distinguished, which, together with type strains, were clustered at a very low level of similarity (24 %). In both single and concatenated analyses of the 16S rRNA gene and ITS sequences, two large groups were formed, with bootstrap support of 99 % in the concatenated analysis. The first group included the type and/or reference strains of Bradyrhizobium japonicum, B. betae, B. liaoningense, B. canariense and B. yuanmingense and B. japonicum USDA 110, and the second group included strains related to Bradyrhizobium elkanii USDA 76T, B. pachyrhizi PAC48T and B. jicamae PAC68T. Similar results were obtained with MLSA of glnII, recA, atpD and dnaK. Greatest variability was observed... Mostrar Tudo |
Thesagro: |
Fixação de nitrogênio; Rhizobium. |
Thesaurus NAL: |
Nitrogen fixation. |
Categoria do assunto: |
V Taxonomia de Organismos |
Marc: |
LEADER 02813naa a2200193 a 4500 001 1657500 005 2017-08-03 008 2009 bl uuuu u00u1 u #d 024 7 $a10.1099/ijs.0.009779-0$2DOI 100 1 $aMENNA, P. 245 $aPhylogeny and taxonomy of a diverse collection of Bradyrhizobium strains based on multilocus sequence analysis of the 16S rRNA gene, ITS region and glnll, recA, atpD and dnaK genes. 260 $c2009 520 $aThe genus Bradyrhizobium encompasses a variety of bacteria that can live in symbiotic and endophytic associations with legumes and non-legumes, and are characterized by physiological and symbiotic versatility and broad geographical distribution. However, despite indications of great genetic variability within the genus, only eight species have been described, mainly because of the highly conserved nature of the 16S rRNA gene. In this study, 169 strains isolated from 43 different legumes were analysed by rep-PCR with the BOX primer, by sequence analysis of the 16S rRNA gene and the 16S?23S rRNA intergenic transcribed spacer (ITS) and by multilocus sequence analysis (MLSA) of four housekeeping genes, glnII, recA, atpD and dnaK. Considering a cut-off at a level of 70 % similarity, 80 rep-PCR profiles were distinguished, which, together with type strains, were clustered at a very low level of similarity (24 %). In both single and concatenated analyses of the 16S rRNA gene and ITS sequences, two large groups were formed, with bootstrap support of 99 % in the concatenated analysis. The first group included the type and/or reference strains of Bradyrhizobium japonicum, B. betae, B. liaoningense, B. canariense and B. yuanmingense and B. japonicum USDA 110, and the second group included strains related to Bradyrhizobium elkanii USDA 76T, B. pachyrhizi PAC48T and B. jicamae PAC68T. Similar results were obtained with MLSA of glnII, recA, atpD and dnaK. Greatest variability was observed when the atpD gene was amplified, and five strains related to B. elkanii revealed a level of variability never reported before. Another important observation was that a group composed of strains USDA 110, SEMIA 5080 and SEMIA 6059, all isolated from soybean, clustered in all six trees with high bootstrap support and were quite distinct from the clusters that included B. japonicum USDA 6T. The results confirm that MLSA is a rapid and reliable way of providing information on phylogenetic relationships and of identifying rhizobial strains potentially representative of novel species. 650 $aNitrogen fixation 650 $aFixação de nitrogênio 650 $aRhizobium 700 1 $aBARCELLOS, F. G. 700 1 $aHUNGRIA, M. 773 $tInternational Journal of Systematic and Evolutionary Microbiology, Great Britain$gv. 59, n. 12, p. 2934-2950, dez. 2009.
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