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| 17. |  | MACEDO, F. A.; CATUNDA, A.; MENEZES, F. B. de; FIGUERO, P. Relação entre o peso da ovelha Morada Nova, var. Vermelha, à parição e o tipo de parto. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 21., 1984, Belo Horizonte. Anais... Belo Horizonte: Sociedade Brasileira de Zootecnia, 1984. p. 82.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 19. | | MENEZES, F. A. B. de; CATUNDA, A. G.; MACHADO, F. H. F.; MACEDO, F. A. R. Comportamento de ovinos deslanados da raça Morada Nova, var. Vermelha, em pastagem nativa melhorada com introdução de capim buffel. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 16., 1979, Curitiba. Anais... Curitiba: Sociedade Brasileira de Zootecnia, 1979. v. 1. p. 45.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
08/11/2019 |
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Data da última atualização: |
08/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
AMARANTE, J. F.; RIBEIRO, M. de F.; COSTA, M. M.; MENEZES, F. G.; SILVA, T. M. S; AMARANTE, T. A. B.; GRADELA, A.; MOURA, L. M. D. |
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Afiliação: |
Jarbas F. Amarante, UNIVASF; MARCIA DE FATIMA RIBEIRO, CPATSA; Mateus M. Costa, UNIVASF; Fredson G. Menezes, UNIVASF; Tania M. S. Silva, UFRPE; Talita A. B. Amarante, UNEB; Adriana Gradela, UNIVASF; Liliane M. D. Moura, UNIVASF. |
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Título: |
Chemical composition and antimicrobial activity of two extract of propolis against isolates of Staphylococcus spp. and multiresistant bacterials. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, v. 39, n. 9, p. 734-743, 2019. |
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DOI: |
10.1590/1678-5150-PVB-6128 |
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Idioma: |
Inglês |
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Conteúdo: |
There is a growing need to discover and develop alternative therapies for the treatment of mastitis caused by Staphylococcus spp. and multidrug-resistant bacterial infections. This study examined the chemical composition and antimicrobial potential of two propolis extracts (EPA and EPB) against seventy-seven isolates of Staphylococcus spp. obtained from subclinical bovine mastitis; three clinical strains of MRSA and two from clinical strains of S. aureus ATCC, identified as S. aureus ATCC 6538 and S. aureus ATCC 25923. The total phenolic content was determined by the Folin-Ciocalteau method, the total flavonoid content by the Dowd method and the phenolic profile was quantified by HPLC-DAD. The MBC values of the extracts were evaluated by broth microdilution method. The amount of total phenolic and flavonoid compounds was higher in EPA than EPB. Both extracts revealed the presence of caffeic, coumaric, cinnamic, ferulic and 3,4-dihydroxybenzoic acids, with higher concentrations of coumaric and cinnamic acids. Staphylococcus spp. isolates were susceptible to EPA (90.9%), EPB (83.1%) and oxacillin (80.5%). The oxacillin susceptible isolates were also susceptible to EPA (70.1%) and EPB (80.6%), whereas those oxacillin-resistant strains were also susceptible to EPA (40.0%) and to EPB (26.7%). MBC ranged from 34.3 to 68.7?m/mL for EPA and from 68.7 to 137.5?g/mL for EPB. Both extracts inhibited significantly (100%) the clinical strains of MRSA, S. aureus ATCC 6538 and S. aureus ATCC 25923 at the concentration of 68.7?g/mL. It is concluded that both extracts of propolis, whose main constituents are coumaric and cinnamic acids, have high antimicrobial activity against the microorganisms studied, and EPA also against oxacillin-resistant strains. These findings reinforce its potential use for the treatment of bovine mastitis. MenosThere is a growing need to discover and develop alternative therapies for the treatment of mastitis caused by Staphylococcus spp. and multidrug-resistant bacterial infections. This study examined the chemical composition and antimicrobial potential of two propolis extracts (EPA and EPB) against seventy-seven isolates of Staphylococcus spp. obtained from subclinical bovine mastitis; three clinical strains of MRSA and two from clinical strains of S. aureus ATCC, identified as S. aureus ATCC 6538 and S. aureus ATCC 25923. The total phenolic content was determined by the Folin-Ciocalteau method, the total flavonoid content by the Dowd method and the phenolic profile was quantified by HPLC-DAD. The MBC values of the extracts were evaluated by broth microdilution method. The amount of total phenolic and flavonoid compounds was higher in EPA than EPB. Both extracts revealed the presence of caffeic, coumaric, cinnamic, ferulic and 3,4-dihydroxybenzoic acids, with higher concentrations of coumaric and cinnamic acids. Staphylococcus spp. isolates were susceptible to EPA (90.9%), EPB (83.1%) and oxacillin (80.5%). The oxacillin susceptible isolates were also susceptible to EPA (70.1%) and EPB (80.6%), whereas those oxacillin-resistant strains were also susceptible to EPA (40.0%) and to EPB (26.7%). MBC ranged from 34.3 to 68.7?m/mL for EPA and from 68.7 to 137.5?g/mL for EPB. Both extracts inhibited significantly (100%) the clinical strains of MRSA, S. aureus ATCC 6538 and S. aureus AT... Mostrar Tudo |
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Palavras-Chave: |
Atividade antimicrobiana; Extrato de própolis; Mastite bovina; Terapia alternativa. |
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Thesagro: |
Bactéria; Composição Química; Doença Animal; Própolis. |
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Thesaurus NAL: |
Animal diseases; Staphylococcus aureus subsp. aureus. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/204415/1/Chemical-composition-and-antimicrobial-activity-2019.pdf
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Marc: |
LEADER 02923naa a2200337 a 4500
001 2114052
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008 2019 bl uuuu u00u1 u #d
024 7 $a10.1590/1678-5150-PVB-6128$2DOI
100 1 $aAMARANTE, J. F.
245 $aChemical composition and antimicrobial activity of two extract of propolis against isolates of Staphylococcus spp. and multiresistant bacterials.$h[electronic resource]
260 $c2019
520 $aThere is a growing need to discover and develop alternative therapies for the treatment of mastitis caused by Staphylococcus spp. and multidrug-resistant bacterial infections. This study examined the chemical composition and antimicrobial potential of two propolis extracts (EPA and EPB) against seventy-seven isolates of Staphylococcus spp. obtained from subclinical bovine mastitis; three clinical strains of MRSA and two from clinical strains of S. aureus ATCC, identified as S. aureus ATCC 6538 and S. aureus ATCC 25923. The total phenolic content was determined by the Folin-Ciocalteau method, the total flavonoid content by the Dowd method and the phenolic profile was quantified by HPLC-DAD. The MBC values of the extracts were evaluated by broth microdilution method. The amount of total phenolic and flavonoid compounds was higher in EPA than EPB. Both extracts revealed the presence of caffeic, coumaric, cinnamic, ferulic and 3,4-dihydroxybenzoic acids, with higher concentrations of coumaric and cinnamic acids. Staphylococcus spp. isolates were susceptible to EPA (90.9%), EPB (83.1%) and oxacillin (80.5%). The oxacillin susceptible isolates were also susceptible to EPA (70.1%) and EPB (80.6%), whereas those oxacillin-resistant strains were also susceptible to EPA (40.0%) and to EPB (26.7%). MBC ranged from 34.3 to 68.7?m/mL for EPA and from 68.7 to 137.5?g/mL for EPB. Both extracts inhibited significantly (100%) the clinical strains of MRSA, S. aureus ATCC 6538 and S. aureus ATCC 25923 at the concentration of 68.7?g/mL. It is concluded that both extracts of propolis, whose main constituents are coumaric and cinnamic acids, have high antimicrobial activity against the microorganisms studied, and EPA also against oxacillin-resistant strains. These findings reinforce its potential use for the treatment of bovine mastitis.
650 $aAnimal diseases
650 $aStaphylococcus aureus subsp. aureus
650 $aBactéria
650 $aComposição Química
650 $aDoença Animal
650 $aPrópolis
653 $aAtividade antimicrobiana
653 $aExtrato de própolis
653 $aMastite bovina
653 $aTerapia alternativa
700 1 $aRIBEIRO, M. de F.
700 1 $aCOSTA, M. M.
700 1 $aMENEZES, F. G.
700 1 $aSILVA, T. M. S
700 1 $aAMARANTE, T. A. B.
700 1 $aGRADELA, A.
700 1 $aMOURA, L. M. D.
773 $tPesquisa Veterinária Brasileira$gv. 39, n. 9, p. 734-743, 2019.
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Embrapa Semiárido (CPATSA) |
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