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Registro Completo |
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
27/12/2020 |
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Data da última atualização: |
26/12/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUSA JUNIOR, L. P. B.; MEIRA, A. N.; AZEVEDO, H. C.; MUNIZ, E. N.; COUTINHO, L. L.; MOURAO, G. B.; LEAO, A. G.; PEDROSA, V. B.; PINTO, L. F. B. |
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Afiliação: |
LUIS PAULO BATISTA SOUSA JUNIOR; ARIANA NASCIMENTO MEIRA; HYMERSON COSTA AZEVEDO, CPATC; EVANDRO NEVES MUNIZ, CPATC; LUIZ LEHMANN COUTINHO; GERSON BARRETO MOURAO; ANDRE GUSTAVO LEAO; VICTOR BRENO PEDROSA; LUIS FERNANDO BATISTA PINTO. |
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Título: |
Variants in myostatin and MyoD family genes are associated with meat quality traits in Santa Inês. sheep |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Animal Biotechnology, v. 33, n. 2, p. 201-213, Apr. 2022. |
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DOI: |
https://doi.org/10.1080/10495398.2020.1781651 |
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Idioma: |
Inglês |
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Notas: |
Epub. 7 Jul. 2020. |
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Conteúdo: |
Myostatin and MyoD family genes play vital roles in myogenesis and this study aimed to identify association of variants in MyoD1, MyoG, MyF5, MyF6, and MSTN genes with meat traits in Santa Inês sheep. A dataset with 44 variants and records of seven meat traits in 192 lambs (pH0, pH24, a, b, L, tenderness assessed by shear force, and water-holding capacity) was used. Single-locus and haplotype association analyses were performed, and the significance threshold was established according to Bonferroni?s method. Single-locus analysis revealed two associations at a Bonferroni level, where the variant c.935-185C > G in MyoD1 had an additive effect (4.31 ± 1.08 N) on tenderness, while the variant c.464 þ 185G > A in MyoG had an additive effect (2.86 ± 0.64) on a. Additionally, the haplotype replacement GT>AC in MSTN was associated with pH0 (1.26 ± 0.31), pH24 (1.07 ± 0.27), a (1.40 ± 0.51), and tenderness (3.83 ± 1.22 N), while the replacement GT > AG in MyoD1 was associated with pH0 (1.43 ± 0.26), pH24 (1.25 ± 0.22), b (1.06 ± 0.39), and tenderness (4.13 ± 1.16 N). Our results have demonstrated that some variants in MyoG, MyF6, MyoD1, and MSTN can be associated with physicochemical meat traits in Santa In^êes sheep. |
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Palavras-Chave: |
Santa Inês. |
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Thesagro: |
Carne; Ovino; Ovinocultura. |
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Thesaurus Nal: |
Lamb meat; Lambs; Sheep. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/219682/1/Variants-in-myostatin.pdf
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Marc: |
LEADER 02183naa a2200325 a 4500
001 2128726
005 2022-12-26
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1080/10495398.2020.1781651$2DOI
100 1 $aSOUSA JUNIOR, L. P. B.
245 $aVariants in myostatin and MyoD family genes are associated with meat quality traits in Santa Inês. sheep$h[electronic resource]
260 $c2022
500 $aEpub. 7 Jul. 2020.
520 $aMyostatin and MyoD family genes play vital roles in myogenesis and this study aimed to identify association of variants in MyoD1, MyoG, MyF5, MyF6, and MSTN genes with meat traits in Santa Inês sheep. A dataset with 44 variants and records of seven meat traits in 192 lambs (pH0, pH24, a, b, L, tenderness assessed by shear force, and water-holding capacity) was used. Single-locus and haplotype association analyses were performed, and the significance threshold was established according to Bonferroni?s method. Single-locus analysis revealed two associations at a Bonferroni level, where the variant c.935-185C > G in MyoD1 had an additive effect (4.31 ± 1.08 N) on tenderness, while the variant c.464 þ 185G > A in MyoG had an additive effect (2.86 ± 0.64) on a. Additionally, the haplotype replacement GT>AC in MSTN was associated with pH0 (1.26 ± 0.31), pH24 (1.07 ± 0.27), a (1.40 ± 0.51), and tenderness (3.83 ± 1.22 N), while the replacement GT > AG in MyoD1 was associated with pH0 (1.43 ± 0.26), pH24 (1.25 ± 0.22), b (1.06 ± 0.39), and tenderness (4.13 ± 1.16 N). Our results have demonstrated that some variants in MyoG, MyF6, MyoD1, and MSTN can be associated with physicochemical meat traits in Santa In^êes sheep.
650 $aLamb meat
650 $aLambs
650 $aSheep
650 $aCarne
650 $aOvino
650 $aOvinocultura
653 $aSanta Inês
700 1 $aMEIRA, A. N.
700 1 $aAZEVEDO, H. C.
700 1 $aMUNIZ, E. N.
700 1 $aCOUTINHO, L. L.
700 1 $aMOURAO, G. B.
700 1 $aLEAO, A. G.
700 1 $aPEDROSA, V. B.
700 1 $aPINTO, L. F. B.
773 $tAnimal Biotechnology$gv. 33, n. 2, p. 201-213, Apr. 2022.
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Registro original: |
Embrapa Tabuleiros Costeiros (CPATC) |
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Biblioteca |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
04/08/2016 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SALIMENA, A. P. S.; LANGE, C. C.; CAMUSSONE, C.; SIGNORINO, M.; CALVINHO, L. F.; BRITO, M. A. V. P. e; BORGES, C. A. V.; GUIMARAES, A. S.; RIBEIRO, J. B.; MENDONCA, L. C.; PICCOLI, R. H. |
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Afiliação: |
Alessandra P.S. Salimena, UFLA; CARLA CHRISTINE LANGE, CNPGL; Cecilia Camussone, INTA - Rafaela; Marcelo Signorini, INTA - Rafaela; Luis F. Calvinho, INTA - Rafaela; MARIA APARECIDA V PAIVA E BRITO, CNPGL; CRISTIANO AMANCIO VIEIRA BORGES, CNPGL; ALESSANDRO DE SA GUIMARAES, CNPGL; JOAO BATISTA RIBEIRO, CNPGL; LETICIA CALDAS MENDONCA, CNPGL; Roberta H. Piccoli, UFLA. |
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Título: |
Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Veterinary Research Communications, v. 40, n. 3/4, p. 97-106, 2016. |
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Páginas: |
10 p. |
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DOI: |
https://doi.org/10.1007/s11259-016-9658-5 |
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Idioma: |
Inglês |
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Conteúdo: |
Staphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. This pathogen produces several virulence factors, including cell-associated adhesins, toxic and cytolytic exoproteins, and capsular polysaccharides. The aim of the present study was to test for the presence of genes involved in capsular polysaccharide production and biofilm formation in S. aureus isolated from bovine mastitis samples collected from 119 dairy herds located in three different Brazilian regions, as well as to assay the production of capsular polysaccharides and biofilm, in vitro. The detection of the cap, icaAD, and bap genes was performed using PCR. The detection and quantification of capsular polysaccharide production was performed using ELISA assays. The ability of the isolates to form a biofilm was examined using the polystyrene surface of microtiter plates. All 159 S. aureus isolates investigated harboured the cap gene: 80 % carried the cap5 gene and 20 % carried the cap8 gene. Sixty-nine percent of the isolates expressed capsular polysaccharide (CP) in vitro, 58 % expressed CP5 and 11 % expressed CP8. All of the isolates harboured the icaA and icaD genes, and 95.6 % of the isolates carried the bap gene. Of the 159 isolates analysed, 97.5 % were biofilm producers. A significant association between the capsular genotype and phenotype and the amount of biofilm formation was detected: cap5/CP5 isolates tended to form more biofilm and to produce a thinner CP layer than cap8/CP8 isolates. The results indicate a high potential for pathogenicity among S. aureus isolated from bovine milk collected from three different regions in Brazil. MenosStaphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. This pathogen produces several virulence factors, including cell-associated adhesins, toxic and cytolytic exoproteins, and capsular polysaccharides. The aim of the present study was to test for the presence of genes involved in capsular polysaccharide production and biofilm formation in S. aureus isolated from bovine mastitis samples collected from 119 dairy herds located in three different Brazilian regions, as well as to assay the production of capsular polysaccharides and biofilm, in vitro. The detection of the cap, icaAD, and bap genes was performed using PCR. The detection and quantification of capsular polysaccharide production was performed using ELISA assays. The ability of the isolates to form a biofilm was examined using the polystyrene surface of microtiter plates. All 159 S. aureus isolates investigated harboured the cap gene: 80 % carried the cap5 gene and 20 % carried the cap8 gene. Sixty-nine percent of the isolates expressed capsular polysaccharide (CP) in vitro, 58 % expressed CP5 and 11 % expressed CP8. All of the isolates harboured the icaA and icaD genes, and 95.6 % of the isolates carried the bap gene. Of the 159 isolates analysed, 97.5 % were biofilm producers. A significant association between the capsular genotype and phenotype and the amount of biofilm formation was detected: cap5/CP5 isolates tended to form more biofilm and to produce a thinner CP layer th... Mostrar Tudo |
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Palavras-Chave: |
Adhesin; Bap; Cap; Capsule; IcaAD. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02672naa a2200325 a 4500
001 2050220
005 2024-02-06
008 2016 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11259-016-9658-5$2DOI
100 1 $aSALIMENA, A. P. S.
245 $aGenotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms.$h[electronic resource]
260 $c2016
300 $a10 p.
520 $aStaphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. This pathogen produces several virulence factors, including cell-associated adhesins, toxic and cytolytic exoproteins, and capsular polysaccharides. The aim of the present study was to test for the presence of genes involved in capsular polysaccharide production and biofilm formation in S. aureus isolated from bovine mastitis samples collected from 119 dairy herds located in three different Brazilian regions, as well as to assay the production of capsular polysaccharides and biofilm, in vitro. The detection of the cap, icaAD, and bap genes was performed using PCR. The detection and quantification of capsular polysaccharide production was performed using ELISA assays. The ability of the isolates to form a biofilm was examined using the polystyrene surface of microtiter plates. All 159 S. aureus isolates investigated harboured the cap gene: 80 % carried the cap5 gene and 20 % carried the cap8 gene. Sixty-nine percent of the isolates expressed capsular polysaccharide (CP) in vitro, 58 % expressed CP5 and 11 % expressed CP8. All of the isolates harboured the icaA and icaD genes, and 95.6 % of the isolates carried the bap gene. Of the 159 isolates analysed, 97.5 % were biofilm producers. A significant association between the capsular genotype and phenotype and the amount of biofilm formation was detected: cap5/CP5 isolates tended to form more biofilm and to produce a thinner CP layer than cap8/CP8 isolates. The results indicate a high potential for pathogenicity among S. aureus isolated from bovine milk collected from three different regions in Brazil.
653 $aAdhesin
653 $aBap
653 $aCap
653 $aCapsule
653 $aIcaAD
700 1 $aLANGE, C. C.
700 1 $aCAMUSSONE, C.
700 1 $aSIGNORINO, M.
700 1 $aCALVINHO, L. F.
700 1 $aBRITO, M. A. V. P. e
700 1 $aBORGES, C. A. V.
700 1 $aGUIMARAES, A. S.
700 1 $aRIBEIRO, J. B.
700 1 $aMENDONCA, L. C.
700 1 $aPICCOLI, R. H.
773 $tVeterinary Research Communications$gv. 40, n. 3/4, p. 97-106, 2016.
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