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Registro Completo |
Biblioteca(s): |
Embrapa Pesca e Aquicultura. |
Data corrente: |
04/10/2018 |
Data da última atualização: |
29/11/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
KIRSCHNIK, L. N. G.; FRANCESCHINI-VICENTINI, I. B.; PAES, M. do C. F.; NAKAGHI, L. S. O. |
Afiliação: |
LUCIANA NAKAGHI GANECO KIRSCHNIK, CNPASA; IRENE BASTOS FRANCESCHINI-VICENTINI, UNESP, Jaboticabal-SP; MARIA DO CARMO FARIA PAES, UNESP, Jaboticabal-SP; LAURA SATIKO OKADA NAKAGHI, UNESP, Jaboticabal-SP. |
Título: |
Embryonic development of teleost Brycon orbignyanus. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Zygote, v. 26, n. 4, p. 294-300, Aug. 2018. |
DOI: |
10.1017/S0967199418000229 |
Idioma: |
Inglês |
Conteúdo: |
Brycon orbignyanus is an important large teleost that is currently on the list of endangered species, therefore studies on its reproductive biology and embryology are fundamental to help species conservation and recovery. The objective of this research was to characterize the events that occur during extrusion, fertilization and embryonic development of the species. The samples were collected at predetermined times, fixed and processed for light microscopy and scanning electron microscopy. The greenish oocytes were spherical, had translucent chorion and a mean diameter of 1.3±0.11 mm. The eggs had well defined animal and vegetative poles approximately 18 min post-fertilization. Stages from 2 to 128 blastomeres occurred between 20 min and 3 h post-fertilization (hPF), when the morula was characterized. The blastula stage was observed between 2 and 3 hPF, and the gastrula between 3 and 7 hPF, when the embryonic shield emerged and the cellular migration with the consequent formation of epiblast and hypoblast. At 8 hPF, the formation of the neural tube, above the notochord and the encephalic region, was observed, delimiting the forebrain, mesencephalon and rhombencephalon regions. From 11 hPF onward, the optic vesicle was formed close to the forebrain and the embryo tail was well developed. The optic vesicle was observed from 12 hPF onward, and the tail showed an intense movement that culminated with the rupture of the chorion and consequent hatching of the larva at 13 hPF and 27°C. MenosBrycon orbignyanus is an important large teleost that is currently on the list of endangered species, therefore studies on its reproductive biology and embryology are fundamental to help species conservation and recovery. The objective of this research was to characterize the events that occur during extrusion, fertilization and embryonic development of the species. The samples were collected at predetermined times, fixed and processed for light microscopy and scanning electron microscopy. The greenish oocytes were spherical, had translucent chorion and a mean diameter of 1.3±0.11 mm. The eggs had well defined animal and vegetative poles approximately 18 min post-fertilization. Stages from 2 to 128 blastomeres occurred between 20 min and 3 h post-fertilization (hPF), when the morula was characterized. The blastula stage was observed between 2 and 3 hPF, and the gastrula between 3 and 7 hPF, when the embryonic shield emerged and the cellular migration with the consequent formation of epiblast and hypoblast. At 8 hPF, the formation of the neural tube, above the notochord and the encephalic region, was observed, delimiting the forebrain, mesencephalon and rhombencephalon regions. From 11 hPF onward, the optic vesicle was formed close to the forebrain and the embryo tail was well developed. The optic vesicle was observed from 12 hPF onward, and the tail showed an intense movement that culminated with the rupture of the chorion and consequent hatching of the larva at 13 hPF and 2... Mostrar Tudo |
Thesagro: |
Brycon Orbignyanus; Histologia Animal; Peixe; Reprodução Animal. |
Thesaurus Nal: |
Embryology; Fish; Histology; Reproduction; Ultrastructure. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02275naa a2200277 a 4500 001 2096898 005 2018-11-29 008 2018 bl uuuu u00u1 u #d 024 7 $a10.1017/S0967199418000229$2DOI 100 1 $aKIRSCHNIK, L. N. G. 245 $aEmbryonic development of teleost Brycon orbignyanus.$h[electronic resource] 260 $c2018 520 $aBrycon orbignyanus is an important large teleost that is currently on the list of endangered species, therefore studies on its reproductive biology and embryology are fundamental to help species conservation and recovery. The objective of this research was to characterize the events that occur during extrusion, fertilization and embryonic development of the species. The samples were collected at predetermined times, fixed and processed for light microscopy and scanning electron microscopy. The greenish oocytes were spherical, had translucent chorion and a mean diameter of 1.3±0.11 mm. The eggs had well defined animal and vegetative poles approximately 18 min post-fertilization. Stages from 2 to 128 blastomeres occurred between 20 min and 3 h post-fertilization (hPF), when the morula was characterized. The blastula stage was observed between 2 and 3 hPF, and the gastrula between 3 and 7 hPF, when the embryonic shield emerged and the cellular migration with the consequent formation of epiblast and hypoblast. At 8 hPF, the formation of the neural tube, above the notochord and the encephalic region, was observed, delimiting the forebrain, mesencephalon and rhombencephalon regions. From 11 hPF onward, the optic vesicle was formed close to the forebrain and the embryo tail was well developed. The optic vesicle was observed from 12 hPF onward, and the tail showed an intense movement that culminated with the rupture of the chorion and consequent hatching of the larva at 13 hPF and 27°C. 650 $aEmbryology 650 $aFish 650 $aHistology 650 $aReproduction 650 $aUltrastructure 650 $aBrycon Orbignyanus 650 $aHistologia Animal 650 $aPeixe 650 $aReprodução Animal 700 1 $aFRANCESCHINI-VICENTINI, I. B. 700 1 $aPAES, M. do C. F. 700 1 $aNAKAGHI, L. S. O. 773 $tZygote$gv. 26, n. 4, p. 294-300, Aug. 2018.
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10. |  | MENDONÇA, J. F. M. de; VALVERDE, I. D.; FONSECA, I.; SILVA M. V.; MARTINS, M. F. Análise das frequências alélicas e genotípicas dos genes para BLAD, DUMPS E CVM em animais da raça Girolando. In: CONGRESSO INTERNACIONAL DA RAÇA GIROLANDO, 1.; CONGRESSO BRASILEIRO DA RAÇA GIROLANDO, 2., 2015, Belo Horizonte. Anais... Belo Horizonte: Associação Brasileira dos Criadores de Girolando, 2015. 5 p.Tipo: Artigo em Anais de Congresso |
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14. |  | MENDONÇA, J. F. M. de; VIEIRA, F. de O.; FONSECA, I.; ARCURI, E. F.; RIBEIRO, J. B.; MARTINS, M. F. Detecção de células viáveis de Salmonella spp. pela técnica de PCR em tempo real. In: CONGRESSO NACIONAL DE LATICÍNIOS, 30., 2015, Juiz de Fora. Anais... Belo Horizonte: EPAMIG, 2015.Tipo: Artigo em Anais de Congresso |
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15. |  | VIEIRA, F. de O.; MENDONÇA, J. F. M. de; FONSECA, I.; ARCURI, E. F.; RIBEIRO, J. B.; MARTINS, M. F. Detecção de células viáveis de Salmonella spp. em queijo Coalho pela técnica de PCR em Tempo Real. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 16., 2015, Juiz de Fora, MG Anais... Juiz de Fora: Embrapa Gado de Leite, 2015. 1 CD-ROM. (Embrapa Gado de Leite. Documentos, 185.). 2 p.Tipo: Resumo em Anais de Congresso |
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16. |  | MENDONÇA, J. F. M. de; SÁ, J. F. O. de; BORGES, M. de F.; MARTINS, M. F. Detecção de patógenos por PCR em tempo real. In: COSTA, R. G. B.; MARTINS, M. F.; MENDONÇA, J. F. M. DE; BORGES, M. DE F. (ed.). Controle de qualidade em queijo minas padrão: métodos físico-químicos, microbiológicos e moleculares. Brasília, DF: Embrapa, 2019. p. 71-82.Tipo: Capítulo em Livro Técnico-Científico |
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18. |  | VIEIRA, F. De O.; MENDONÇA, J. F. M. De; FONSECA, I.; ARCURI, E. F.; RIBEIRO, J. B.; MARTINS, M. F. EMA-PCR: detection only viable pathogen on cell culture by Real-Time PCR. In: CONGRESSO BRASILEIRO DE GENÉTICA, 62., 2016, Caxambu. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2016.Tipo: Resumo em Anais de Congresso |
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