Data/hora: 23/09/2024 17:06:04

Biblioteca(s): 	Embrapa Gado de Leite.	
Data corrente: 	10/11/2022	
Data da última atualização: 	12/12/2022	
Autoria: 	MARQUES-BASTOS, S. L. S.; COELHO, M. L. V.; SANTOS, I. N. de S.; MORENO, D. S. A.; BARRIAS, E. S.; MENDONÇA, J. F. M. de; MENDONCA, L. C.; LANGE, C. C.; BRITO, M. A. V. de P.; BASTOS, M. do C. de F.	
Afiliação: 	SELDA LOASE SALUSTIANO MARQUES-BASTOS, Universidade Federal do Rio de Janeiro; MARCUS LÍVIO VARELLA COELHO, Universidade Federal do Rio de Janeiro; ILANA NASCIMENTO DE SOUSA SANTOS, Universidade Federal do Rio de Janeiro; DANIELA SALES ALVIANO MORENO, Universidade Federal do Rio de Janeiro; EMILE SANTOS BARRIAS, Instituto Nacional de Metrologia, Qualidade E Tecnologia; JULIANA FRANÇA MONTEIRO DE MENDONÇA; LETICIA CALDAS MENDONCA, CNPGL; CARLA CHRISTINE LANGE, CNPGL; MARIA APARECIDA VASCONCELOS DE PAIVA BRITO; MARIA DO CARMO DE FREIRE BASTOS, Universidade Federal do Rio de Janeiro.	
Título: 	Effects of the natural antimicrobial peptide aureocin A53 on cells of Staphylococcus aureus and Streptococcus agalactiae involved in bovine mastitis in the excised teat model.	
Ano de publicação: 	2023	
Fonte/Imprenta: 	World Journal of Microbiology & Biotechnology, v. 39, n. 1, article 5, 2023.	
DOI: 	https://doi.org/10.1007/s11274-022-03443-w	
Idioma: 	Inglês	
Conteúdo: 	Aureocin A53 is an N-formylated antimicrobial peptide (AMP) produced by Staphylococcus aureus. Aureocin A53 has a broad spectrum of antimicrobial activity against human and animal pathogens. In the present study, its antagonistic activity was investigated towards 30 strains of S. aureus and 30 strains of Streptococcus spp. isolated from bovine mastitis cases in Brazil. Bovine mastitis is a disease that causes a major economic impact worldwide. Aureocin A53 inhibited the growth of all 60 strains tested, including multidrug-resistant streptococcal isolates and strains of S. aureus belonging to different pulsotypes. This AMP proved to be bactericidal against the six target strains randomly selected among staphylococci and streptococci, also exhibiting a lytic mode of action against the staphylococcal cells. Furthermore, it was determined that 2,048 AU/mL of the AMP were required to inhibit 99.99% of the cell growth of the strain less sensitive to aureocin A53. Aureocin A53 was not toxic to bovine mammary gland epithelial cells after a 24-h exposure and maintained its antimicrobial activity when tested in the excised-teat model against strains of S. aureus and Streptococcus agalactiae, the species responsible for most intramammary infections, not only in Brazil but in other countries as well. Therefore, the use of aureocin A53 in the development of new pharmacological products for the prophylaxis and/or treatment of bovine mastitis was considered promising.	
Palavras-Chave: 	Aureocina A53; Mastite; Peptídeo antimicrobiano.	
Thesagro: 	Bovino; Estreptococo.	
Thesaurus Nal: 	Antimicrobial peptides; Bovine mastitis; Staphylococcus aureus subsp. aureus; Streptococcus.	
Categoria do assunto: 	L Ciência Animal e Produtos de Origem Animal	
Registro original: 	Embrapa Gado de Leite (CNPGL)	
Exemplares: 	CNPGL - (25800-1) - UPC - AP - DD -  -  -  - DIS