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Registro Completo |
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
18/11/2022 |
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Data da última atualização: |
17/12/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
THEODORO, J. M. V.; GRANCIERI, M.; GOMES, M. J. C.; TOLEDO, R. C. L.; SÃO JOSÉ, V. P. B. DE; MANTOVANI, H. C.; CARVALHO, C. W. P. de; SILVA, B. P. DA; MARTINO, H. S. D. |
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Afiliação: |
JAQUELINE MACIEL VIEIRA THEODORO, UFV; MARIANA GRANCIERI, UFV, UFES; MARIANA JUSTE CONTIN GOMES, UFV; RENATA CELI LOPES TOLEDO, UFV; VINÍCIUS PARZANINI BRILHANTE DE SÃO JOSÉ, UFV; HILÁRIO CUQUETTO MANTOVANI, UFV; CARLOS WANDERLEI PILER DE CARVALHO, CTAA; BÁRBARA PEREIRA DA SILVA, UFV; HÉRCIA STAMPINI DUARTE MARTINO, UFV. |
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Título: |
Germinated millet (Pennisetum glaucum (L.) R. Br.) flour improved the gut function and its microbiota composition in rats fed with high-fat high-fructose diet. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
International Journal Of Environmental Research And Public Health, v. 19, n. 22, 15217, p. 1-14, 2022. |
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DOI: |
https://doi.org/10.3390/ijerph192215217 |
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Idioma: |
Inglês |
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Conteúdo: |
Germinated millet (Pennisetum glaucum (L.) R. Br.) is a source of phenolic compounds that has potential prebiotic action. This study aims at evaluating the action of germinated pearl millet on gut function and its microbiota composition in Wistar rats fed with a high-fat high-fructose (HFHF) diet. In the first stage, lasting eight weeks, the experiment consisted of two groups: AIN93M (n = 10) and HFHF group (n = 20). In the second stage, which lasted ten weeks, the animals of the AIN-93M group (n = 10) were kept, while the HFHF group was dismembered into HFHF (HFHF diet, n = 10) and HFHF + millet (HFHF added 28.6% of germinated millet flour, n = 10) groups. After the 18th week, the urine of the animals was collected for the analysis of lactulose and mannitol intestinal permeability by urinary excretion. The histomorphometry was analyzed on the proximal colon and the fecal pH, concentration of short-chain fatty acids (SCFA), and sequencing of microbiota were performed in cecum content. The Mothur v.1.44.3 software was used for data analysis of sequencing. Alpha diversity was estimated by Chao1, Shannon, and Simpson indexes. Beta diversity was assessed by PCoA (Principal Coordinate Analysis). The functional predictive analysis was performed with PICRUSt2 software (version 2.1.2?b). Functional traits attributed to normalized OTU abundance were determined by the Kyoto Encyclopedia of Genes and Genomes (KEGG). In the results, germinated millet flour reduced Oscillibacter genus and Desulfobacterota phylum, while increasing the Eggerthellaceae family. Furthermore, germinated millet flour: increased beta diversity, cecum weight, and cecum/body weight ratio; improved gut histological parameters by increasing the depth and thickness of the crypt and the goblet cell count (p < 0.05); reduced (p < 0.05) the fecal pH and mannitol urinary excretion; increased (p < 0.05) the propionate short-chain fatty acid concentration. Thus, germinated millet has the potential to improve the composition of gut microbiota and the intestinal function of rats fed with an HFHF diet. MenosGerminated millet (Pennisetum glaucum (L.) R. Br.) is a source of phenolic compounds that has potential prebiotic action. This study aims at evaluating the action of germinated pearl millet on gut function and its microbiota composition in Wistar rats fed with a high-fat high-fructose (HFHF) diet. In the first stage, lasting eight weeks, the experiment consisted of two groups: AIN93M (n = 10) and HFHF group (n = 20). In the second stage, which lasted ten weeks, the animals of the AIN-93M group (n = 10) were kept, while the HFHF group was dismembered into HFHF (HFHF diet, n = 10) and HFHF + millet (HFHF added 28.6% of germinated millet flour, n = 10) groups. After the 18th week, the urine of the animals was collected for the analysis of lactulose and mannitol intestinal permeability by urinary excretion. The histomorphometry was analyzed on the proximal colon and the fecal pH, concentration of short-chain fatty acids (SCFA), and sequencing of microbiota were performed in cecum content. The Mothur v.1.44.3 software was used for data analysis of sequencing. Alpha diversity was estimated by Chao1, Shannon, and Simpson indexes. Beta diversity was assessed by PCoA (Principal Coordinate Analysis). The functional predictive analysis was performed with PICRUSt2 software (version 2.1.2?b). Functional traits attributed to normalized OTU abundance were determined by the Kyoto Encyclopedia of Genes and Genomes (KEGG). In the results, germinated millet flour reduced Oscillibacter genus an... Mostrar Tudo |
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Palavras-Chave: |
Alimentos integrais; Beta diversity; Intestinal permeability; Prebióticos; Saúde intestinal. |
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Thesagro: |
Tecnologia de Alimento. |
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Thesaurus Nal: |
Food technology; Goblet cells; Prebiotics; Whole grain foods. |
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Categoria do assunto: |
Q Alimentos e Nutrição Humana |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148424/1/ijerph-19-15217.pdf
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Marc: |
LEADER 03270naa a2200349 a 4500
001 2148424
005 2022-12-17
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/ijerph192215217$2DOI
100 1 $aTHEODORO, J. M. V.
245 $aGerminated millet (Pennisetum glaucum (L.) R. Br.) flour improved the gut function and its microbiota composition in rats fed with high-fat high-fructose diet.$h[electronic resource]
260 $c2022
520 $aGerminated millet (Pennisetum glaucum (L.) R. Br.) is a source of phenolic compounds that has potential prebiotic action. This study aims at evaluating the action of germinated pearl millet on gut function and its microbiota composition in Wistar rats fed with a high-fat high-fructose (HFHF) diet. In the first stage, lasting eight weeks, the experiment consisted of two groups: AIN93M (n = 10) and HFHF group (n = 20). In the second stage, which lasted ten weeks, the animals of the AIN-93M group (n = 10) were kept, while the HFHF group was dismembered into HFHF (HFHF diet, n = 10) and HFHF + millet (HFHF added 28.6% of germinated millet flour, n = 10) groups. After the 18th week, the urine of the animals was collected for the analysis of lactulose and mannitol intestinal permeability by urinary excretion. The histomorphometry was analyzed on the proximal colon and the fecal pH, concentration of short-chain fatty acids (SCFA), and sequencing of microbiota were performed in cecum content. The Mothur v.1.44.3 software was used for data analysis of sequencing. Alpha diversity was estimated by Chao1, Shannon, and Simpson indexes. Beta diversity was assessed by PCoA (Principal Coordinate Analysis). The functional predictive analysis was performed with PICRUSt2 software (version 2.1.2?b). Functional traits attributed to normalized OTU abundance were determined by the Kyoto Encyclopedia of Genes and Genomes (KEGG). In the results, germinated millet flour reduced Oscillibacter genus and Desulfobacterota phylum, while increasing the Eggerthellaceae family. Furthermore, germinated millet flour: increased beta diversity, cecum weight, and cecum/body weight ratio; improved gut histological parameters by increasing the depth and thickness of the crypt and the goblet cell count (p < 0.05); reduced (p < 0.05) the fecal pH and mannitol urinary excretion; increased (p < 0.05) the propionate short-chain fatty acid concentration. Thus, germinated millet has the potential to improve the composition of gut microbiota and the intestinal function of rats fed with an HFHF diet.
650 $aFood technology
650 $aGoblet cells
650 $aPrebiotics
650 $aWhole grain foods
650 $aTecnologia de Alimento
653 $aAlimentos integrais
653 $aBeta diversity
653 $aIntestinal permeability
653 $aPrebióticos
653 $aSaúde intestinal
700 1 $aGRANCIERI, M.
700 1 $aGOMES, M. J. C.
700 1 $aTOLEDO, R. C. L.
700 1 $aSÃO JOSÉ, V. P. B. DE
700 1 $aMANTOVANI, H. C.
700 1 $aCARVALHO, C. W. P. de
700 1 $aSILVA, B. P. DA
700 1 $aMARTINO, H. S. D.
773 $tInternational Journal Of Environmental Research And Public Health$gv. 19, n. 22, 15217, p. 1-14, 2022.
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Embrapa Agroindústria de Alimentos (CTAA) |
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