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| Registros recuperados : 2 | |
| 1. |  | TOMICH, T. R.; RODRIGUES, J. A. S.; GONCALVES, L. C.; PINTO, R. G.; PENNA, A. G.; BORGES, A. L. C. C.; BORGES, I.; GLORIA, I. R. da. Produções de matéria natural e de matéria seca e relação folha/colmo de vinte e cinco híbridos de sorgo (Sorghum bicolor) com capim sudão (Sorghum sudanense) utilizados para produção de forragem em regime de corte. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 39., 2002, Recife. A produção animal e a sociedade brasileira: anais. Recife: UFRPE: SBZ, 2002. 1 CD-ROM.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 2. | | RIBAS, M. N.; GONÇALVES, L. C.; IBRAHIM, G. H. F.; RODRIGUEZ, N. M.; BORGES, I.; BORGTES, A. L. C. C.; RODRIGUES, J. A. S.; PÔSSAS, F. P.; GLORIA, I. R. da. Consumo e digestibilidade aparente das frações fibrosas das silagens de quatro genótipos de milho. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 43., 2006, João Pessoa. Anais... João Pessoa: SBZ: UFPB, 2006. 1 CD-ROM.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 2 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
10/01/2020 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
CAMARGO, L. S. de A.; AGUIRRE-LAVIN, T.; ADENOT, P.; ARAUJO, T. D.; MENDES, V. R. A.; LOURO, I. D.; BEAUJEAN, N.; SOUZA, E. D. |
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Afiliação: |
LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; Tiphaine Aguirre-Lavin; Pierre Adenot; Thamiris Dornelas Araujo; Vivian Rachel Araujo Mendes; Iuri Drumond Louro; Nathalie Beaujean; Eliza Diniz Souza. |
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Título: |
Heat shock during in vitro maturation induces chromatin modifications in the bovine embryo. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Reproduction, v. 158, n. 4, p. 313-322, 2019. |
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DOI: |
https://doi.org/10.1530/REP-19-0245 |
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Idioma: |
Inglês |
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Conteúdo: |
Heat stress compromises bovine oocyte developmental competence but the effects of high temperature during oocyte maturation on embryo chromatin organization is unknown. In this study bovine oocytes were exposed to heat shock (41°C) for 12h during in vitro maturation and then submitted to in vitro fertilization. The heat shock did not affect (P>0.05) the cleavage but reduced (P<0.01) the blastocyst rate on Day 7 and Day 8. No effect (P>0.05) on total cell number was found but the heat shock increased (P<0.05) the proportion of apoptotic cells in blastocysts at Day 8. Immunofluorescence analysis of H3K9me3 and HP1 was performed in embryos at 52 h post in vitro fertilization. An accumulation of H3K9me3 in the nuclei of embryos derived from heat-shocked oocytes at 4-cell and 8-cell stages was found. Also, a non-expected higher proportion (P<0.05) of 4-cell stage embryos displaying nuclei with increased HP1 fluorescence was observed, suggesting an abnormal chromatin compaction in embryos from heat-shocked oocytes. Embryos at 8-cell stage derived from heat-shocked oocytes displayed lower (P<0.05) relative amount of HSP40 transcripts than control ones. In conclusion, heat shock before fertilization has an effect on embryo chromatin, influencing the accumulation of H3K9me3 and HP1 in early embryos as well as further development. |
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Palavras-Chave: |
Embryo chromatin; Heat shock. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02073naa a2200241 a 4500
001 2118579
005 2024-02-06
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1530/REP-19-0245$2DOI
100 1 $aCAMARGO, L. S. de A.
245 $aHeat shock during in vitro maturation induces chromatin modifications in the bovine embryo.$h[electronic resource]
260 $c2019
520 $aHeat stress compromises bovine oocyte developmental competence but the effects of high temperature during oocyte maturation on embryo chromatin organization is unknown. In this study bovine oocytes were exposed to heat shock (41°C) for 12h during in vitro maturation and then submitted to in vitro fertilization. The heat shock did not affect (P>0.05) the cleavage but reduced (P<0.01) the blastocyst rate on Day 7 and Day 8. No effect (P>0.05) on total cell number was found but the heat shock increased (P<0.05) the proportion of apoptotic cells in blastocysts at Day 8. Immunofluorescence analysis of H3K9me3 and HP1 was performed in embryos at 52 h post in vitro fertilization. An accumulation of H3K9me3 in the nuclei of embryos derived from heat-shocked oocytes at 4-cell and 8-cell stages was found. Also, a non-expected higher proportion (P<0.05) of 4-cell stage embryos displaying nuclei with increased HP1 fluorescence was observed, suggesting an abnormal chromatin compaction in embryos from heat-shocked oocytes. Embryos at 8-cell stage derived from heat-shocked oocytes displayed lower (P<0.05) relative amount of HSP40 transcripts than control ones. In conclusion, heat shock before fertilization has an effect on embryo chromatin, influencing the accumulation of H3K9me3 and HP1 in early embryos as well as further development.
653 $aEmbryo chromatin
653 $aHeat shock
700 1 $aAGUIRRE-LAVIN, T.
700 1 $aADENOT, P.
700 1 $aARAUJO, T. D.
700 1 $aMENDES, V. R. A.
700 1 $aLOURO, I. D.
700 1 $aBEAUJEAN, N.
700 1 $aSOUZA, E. D.
773 $tReproduction$gv. 158, n. 4, p. 313-322, 2019.
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