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Registro Completo |
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
27/12/2017 |
Data da última atualização: |
03/01/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
POLESE, V.; SOARES, C. de P.; SILVA, P. R. A. da; ARAUJO, J. L. S. de; BALDANI, J. I.; VIDAL, M. S. |
Afiliação: |
VALÉRIA POLESE, UFRRJ; LEITON DE PAULA SOARES, UFRRJ; PAULA RENATA ALVES DA SILVA, UFRRJ; JEAN LUIZ SIMOES DE ARAUJO, CNPAB; JOSE IVO BALDANI, CNPAB; MARCIA SOARES VIDAL, CNPAB. |
Título: |
Selection and validation of reference genes for RT-qPCR indicates that juice of sugarcane varieties modulate the expression of C metabolism genes in the endophytic diazotrophic Herbaspirillum rubrisubalbicans strain HCC103. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Antonie van Leeuwenhoekv, 110, n. 12, p. 1555-1568, 2017. |
ISSN: |
1572-9699 |
DOI: |
https://doi.org/10.1007/s10482-017-0906-7 |
Idioma: |
Inglês |
Conteúdo: |
Quantitative reverse transcription PCR (RT-qPCR) is an important tool for evaluating gene expression. However, this technique requires that specific internal normalizing genes be identified for different experimental conditions. To date, no internal normalizing genes are available for validation of data analyses for Herbaspirillum rubrisubalbicans strain HCC103, an endophyte that is part of the sugarcane consortium inoculant. This work seeks to identify and evaluate suitable reference genes for gene expression studies in HCC103 grown until middle log phase in sugarcane juice obtained from four sugarcane varieties or media with three different carbon sources. The mRNA levels of five candidate genes (rpoA, gyrA, dnaG, recA and gmK) and seven target genes involved in carbon metabolism (acnA, fbp, galE, suhB, wcaA, ORF_0127.0101 and _0127.0123) were quantified by RT-qPCR. Analysis of expression stability of these genes was carried out using geNorm and Normfinder software. The results indicated that the HCC103 dnaG and gyrA genes are the most stable and showed adequate relative expression level changes among the different sugarcane juices. The highest expression level was seen for ORF_0127.0101, which encodes a sugar transporter, in juice from sugarcane variety RB867515 and glucose as the carbon source. The suhB gene, encoding SuhB inositol monophosphatase, had a higher relative expression level on 0.5% glucose, 100% sugarcane juice from variety RB867515 and 0.5% aconitate. Together the results suggest that dnaG and gyrA genes are suitable as reference genes for RT-qPCR analysis of strain HCC103 and that juice from different sugarcane varieties modulates the expression of key genes involved in carbon metabolism. MenosQuantitative reverse transcription PCR (RT-qPCR) is an important tool for evaluating gene expression. However, this technique requires that specific internal normalizing genes be identified for different experimental conditions. To date, no internal normalizing genes are available for validation of data analyses for Herbaspirillum rubrisubalbicans strain HCC103, an endophyte that is part of the sugarcane consortium inoculant. This work seeks to identify and evaluate suitable reference genes for gene expression studies in HCC103 grown until middle log phase in sugarcane juice obtained from four sugarcane varieties or media with three different carbon sources. The mRNA levels of five candidate genes (rpoA, gyrA, dnaG, recA and gmK) and seven target genes involved in carbon metabolism (acnA, fbp, galE, suhB, wcaA, ORF_0127.0101 and _0127.0123) were quantified by RT-qPCR. Analysis of expression stability of these genes was carried out using geNorm and Normfinder software. The results indicated that the HCC103 dnaG and gyrA genes are the most stable and showed adequate relative expression level changes among the different sugarcane juices. The highest expression level was seen for ORF_0127.0101, which encodes a sugar transporter, in juice from sugarcane variety RB867515 and glucose as the carbon source. The suhB gene, encoding SuhB inositol monophosphatase, had a higher relative expression level on 0.5% glucose, 100% sugarcane juice from variety RB867515 and 0.5% aconitate. Toget... Mostrar Tudo |
Palavras-Chave: |
Internal normalizing; Organic acids; Relative expression; Sugar transport. |
Thesaurus Nal: |
nitrogen fixation. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02682naa a2200265 a 4500 001 2083708 005 2018-01-03 008 2017 bl uuuu u00u1 u #d 022 $a1572-9699 024 7 $ahttps://doi.org/10.1007/s10482-017-0906-7$2DOI 100 1 $aPOLESE, V. 245 $aSelection and validation of reference genes for RT-qPCR indicates that juice of sugarcane varieties modulate the expression of C metabolism genes in the endophytic diazotrophic Herbaspirillum rubrisubalbicans strain HCC103.$h[electronic resource] 260 $c2017 520 $aQuantitative reverse transcription PCR (RT-qPCR) is an important tool for evaluating gene expression. However, this technique requires that specific internal normalizing genes be identified for different experimental conditions. To date, no internal normalizing genes are available for validation of data analyses for Herbaspirillum rubrisubalbicans strain HCC103, an endophyte that is part of the sugarcane consortium inoculant. This work seeks to identify and evaluate suitable reference genes for gene expression studies in HCC103 grown until middle log phase in sugarcane juice obtained from four sugarcane varieties or media with three different carbon sources. The mRNA levels of five candidate genes (rpoA, gyrA, dnaG, recA and gmK) and seven target genes involved in carbon metabolism (acnA, fbp, galE, suhB, wcaA, ORF_0127.0101 and _0127.0123) were quantified by RT-qPCR. Analysis of expression stability of these genes was carried out using geNorm and Normfinder software. The results indicated that the HCC103 dnaG and gyrA genes are the most stable and showed adequate relative expression level changes among the different sugarcane juices. The highest expression level was seen for ORF_0127.0101, which encodes a sugar transporter, in juice from sugarcane variety RB867515 and glucose as the carbon source. The suhB gene, encoding SuhB inositol monophosphatase, had a higher relative expression level on 0.5% glucose, 100% sugarcane juice from variety RB867515 and 0.5% aconitate. Together the results suggest that dnaG and gyrA genes are suitable as reference genes for RT-qPCR analysis of strain HCC103 and that juice from different sugarcane varieties modulates the expression of key genes involved in carbon metabolism. 650 $anitrogen fixation 653 $aInternal normalizing 653 $aOrganic acids 653 $aRelative expression 653 $aSugar transport 700 1 $aSOARES, C. de P. 700 1 $aSILVA, P. R. A. da 700 1 $aARAUJO, J. L. S. de 700 1 $aBALDANI, J. I. 700 1 $aVIDAL, M. S. 773 $tAntonie van Leeuwenhoekv, 110$gn. 12, p. 1555-1568, 2017.
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Embrapa Agrobiologia (CNPAB) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Hortaliças. |
Data corrente: |
11/08/1997 |
Data da última atualização: |
11/08/1997 |
Autoria: |
MENEZES, J. E. de; HENZ, G. P.; PESSOA, H. B. S. V. |
Afiliação: |
EMBRAPA-CNPH, Brasilia, DF. |
Título: |
Qualidade fisiologica e sanitaria de sementes de cenoura cv. Brasilia produzidas no Distrito Federal. |
Ano de publicação: |
1997 |
Fonte/Imprenta: |
Horticultura Brasileira, Brasilia, v.15, 1997. |
Idioma: |
Português |
Notas: |
Resumo 178. Suplemento. |
Palavras-Chave: |
Brasil; Brasilia; Distrito Federal; Qualidade fisiologica; Qualidade sanitaria; Quality; Variedade Brasilia. |
Thesagro: |
Cenoura; Cerrado; Daucus Carota; Semente. |
Thesaurus NAL: |
Brazil; carrots; seeds. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00847naa a2200313 a 4500 001 1758263 005 1997-08-11 008 1997 bl uuuu u00u1 u #d 100 1 $aMENEZES, J. E. de 245 $aQualidade fisiologica e sanitaria de sementes de cenoura cv. Brasilia produzidas no Distrito Federal. 260 $c1997 500 $aResumo 178. Suplemento. 650 $aBrazil 650 $acarrots 650 $aseeds 650 $aCenoura 650 $aCerrado 650 $aDaucus Carota 650 $aSemente 653 $aBrasil 653 $aBrasilia 653 $aDistrito Federal 653 $aQualidade fisiologica 653 $aQualidade sanitaria 653 $aQuality 653 $aVariedade Brasilia 700 1 $aHENZ, G. P. 700 1 $aPESSOA, H. B. S. V. 773 $tHorticultura Brasileira, Brasilia$gv.15, 1997.
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