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 | Acesso ao texto completo restrito à biblioteca da Embrapa Solos. Para informações adicionais entre em contato com cnps.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
13/07/2011 |
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Data da última atualização: |
06/05/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SONG, G.; HAYES, M. H. B.; NOVOTNY, E. H.; SIMPSON, A. J. |
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Afiliação: |
GUIXUE SONG, UNIVERSIDADE DE LIMERICK; MICHAEL H. B. HAYES, UNIVERSIDADE DE LIMERICK; ETELVINO HENRIQUE NOVOTNY, CNPS; ANDRE J. SIMPSON, UNIVERSIDADE DE TORONTO. |
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Título: |
Isolation and fractionation of soil humin using alkaline urea and dimethylsulphoxide plus sulphuric acid. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Naturwissenschaften, v. 98, p. 7-13, 2011. |
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DOI: |
https://doi.org/10.1007/s00114-010-0733-4 |
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Idioma: |
Inglês |
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Conteúdo: |
Humin, the most recalcitrant and abundant organic fraction of soils and of sediments, is a significant contributor to the stable carbon pool in soils and is important for the global carbon budget. It has significant resistance to transformations by microorganisms. Based on the classical operational definition, humin can include any humic-type substance that is not soluble in water at any pH. We demonstrate in this study how sequential exhaustive extractions with 0.1 M sodium hydroxide (NaOH) + 6 M urea, followed by dimethylsulphoxide (DMSO) + 6% (v/v) sulphuric acid (H2SO4) solvent systems, can extract 70–80% of the residual materials remaining after prior exhaustive extractions in neutral and aqueous basic media. Solid-state 13C NMR spectra have shown that the components isolated in the base + urea system were compositionally similar to the humic and fulvic acid fractions isolated at pH 12.6 in the aqueous media. The NMR spectra indicated that the major components isolated in the DMSO + H2SO4 medium had aliphatic hydrocarbon associated with carboxyl functionalities and with lesser amounts of carbohydrate and peptide and minor amounts of lignin-derived components. The major components will have significant contributions from long-chain fatty acids, waxes, to cuticular materials. The isolates in the DMSO + H2SO4 medium were compositionally similar to the organic components that resisted solvation and remained associated with the soil clays. It is concluded that the base + urea system released humic and fulvic acids held by hydrogen bonding or by entrapment within the humin matrix. The recalcitrant humin materials extracted in DMSO + H2SO4 are largely biological molecules (from plants and the soil microbial population) that are likely to be protected from degradation by their hydrophobic moieties and by sorption on the soil clays. Thus, the major components of humin do not satisfy the classical definitions for humic substances which emphasise that these arise from microbial or chemical transformations in soils of the components of organic debris. MenosHumin, the most recalcitrant and abundant organic fraction of soils and of sediments, is a significant contributor to the stable carbon pool in soils and is important for the global carbon budget. It has significant resistance to transformations by microorganisms. Based on the classical operational definition, humin can include any humic-type substance that is not soluble in water at any pH. We demonstrate in this study how sequential exhaustive extractions with 0.1 M sodium hydroxide (NaOH) + 6 M urea, followed by dimethylsulphoxide (DMSO) + 6% (v/v) sulphuric acid (H2SO4) solvent systems, can extract 70–80% of the residual materials remaining after prior exhaustive extractions in neutral and aqueous basic media. Solid-state 13C NMR spectra have shown that the components isolated in the base + urea system were compositionally similar to the humic and fulvic acid fractions isolated at pH 12.6 in the aqueous media. The NMR spectra indicated that the major components isolated in the DMSO + H2SO4 medium had aliphatic hydrocarbon associated with carboxyl functionalities and with lesser amounts of carbohydrate and peptide and minor amounts of lignin-derived components. The major components will have significant contributions from long-chain fatty acids, waxes, to cuticular materials. The isolates in the DMSO + H2SO4 medium were compositionally similar to the organic components that resisted solvation and remained associated with the soil clays. It is concluded that the base + ure... Mostrar Tudo |
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Palavras-Chave: |
Soil humin. |
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Thesaurus Nal: |
soil organic matter. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 02719naa a2200193 a 4500
001 1895801
005 2024-05-06
008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s00114-010-0733-4$2DOI
100 1 $aSONG, G.
245 $aIsolation and fractionation of soil humin using alkaline urea and dimethylsulphoxide plus sulphuric acid.$h[electronic resource]
260 $c2011
520 $aHumin, the most recalcitrant and abundant organic fraction of soils and of sediments, is a significant contributor to the stable carbon pool in soils and is important for the global carbon budget. It has significant resistance to transformations by microorganisms. Based on the classical operational definition, humin can include any humic-type substance that is not soluble in water at any pH. We demonstrate in this study how sequential exhaustive extractions with 0.1 M sodium hydroxide (NaOH) + 6 M urea, followed by dimethylsulphoxide (DMSO) + 6% (v/v) sulphuric acid (H2SO4) solvent systems, can extract 70–80% of the residual materials remaining after prior exhaustive extractions in neutral and aqueous basic media. Solid-state 13C NMR spectra have shown that the components isolated in the base + urea system were compositionally similar to the humic and fulvic acid fractions isolated at pH 12.6 in the aqueous media. The NMR spectra indicated that the major components isolated in the DMSO + H2SO4 medium had aliphatic hydrocarbon associated with carboxyl functionalities and with lesser amounts of carbohydrate and peptide and minor amounts of lignin-derived components. The major components will have significant contributions from long-chain fatty acids, waxes, to cuticular materials. The isolates in the DMSO + H2SO4 medium were compositionally similar to the organic components that resisted solvation and remained associated with the soil clays. It is concluded that the base + urea system released humic and fulvic acids held by hydrogen bonding or by entrapment within the humin matrix. The recalcitrant humin materials extracted in DMSO + H2SO4 are largely biological molecules (from plants and the soil microbial population) that are likely to be protected from degradation by their hydrophobic moieties and by sorption on the soil clays. Thus, the major components of humin do not satisfy the classical definitions for humic substances which emphasise that these arise from microbial or chemical transformations in soils of the components of organic debris.
650 $asoil organic matter
653 $aSoil humin
700 1 $aHAYES, M. H. B.
700 1 $aNOVOTNY, E. H.
700 1 $aSIMPSON, A. J.
773 $tNaturwissenschaften$gv. 98, p. 7-13, 2011.
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Embrapa Solos (CNPS) |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
07/12/2004 |
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Data da última atualização: |
01/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MELO, L. C.; SANTOS, J. B. dos; FERREIRA, D. F. |
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Afiliação: |
LEONARDO CUNHA MELO, CNPAF; JOAO BATISTA DOS SANTOS, UFLA; DANIEL FURTADO FERREIRA, UFLA. |
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Título: |
QTL mapping for common bean grain yield in different environments. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, v. 4, n. 2, p. 135-144, June 2004. |
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Idioma: |
Inglês |
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Conteúdo: |
Os objetivos desse estudo foram identificar, em diferentes épocas e locais QTLs de produtividade de grãos em feijoeiro comum, avaliar as interações de QTLs por ambientes e comparar os métodos de mapeamento e regressão múltipla. Foram avaliadas 196 linhagens recombinantes do cruzamento 'Carioca' x 'Flor de Mayo' nos anos de 1996, 1997 e 1998, em duas épocas e dois locais. Para a avaliação fenotípica foram conduzidos sete experimentos em látice quadrado simples 14 x 14. A interação QTLs por locais foi expressiva, mas foram identificados alguns QTLs com estabilidade. Não houve concordância entre os resultados apresentados pelos dois métodos de mapeamento. Os marcadores que se mostraram mais estáveis e promissores para serem utilizados na seleção assistida para produtividade de grãos foram OPO-19 (1412 pb), OPO-20 (1585 pb) e OPN-07 (1445 pb). |
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Palavras-Chave: |
Ambiente; Interação QTLs; Mapeamento; Marcadores RAPD. |
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Thesagro: |
Feijão; Phaseolus Vulgaris; Produção. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/212718/1/cbab-2004.pdf
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Marc: |
LEADER 01527naa a2200229 a 4500
001 1212718
005 2022-06-01
008 2004 bl uuuu u00u1 u #d
100 1 $aMELO, L. C.
245 $aQTL mapping for common bean grain yield in different environments.$h[electronic resource]
260 $c2004
520 $aOs objetivos desse estudo foram identificar, em diferentes épocas e locais QTLs de produtividade de grãos em feijoeiro comum, avaliar as interações de QTLs por ambientes e comparar os métodos de mapeamento e regressão múltipla. Foram avaliadas 196 linhagens recombinantes do cruzamento 'Carioca' x 'Flor de Mayo' nos anos de 1996, 1997 e 1998, em duas épocas e dois locais. Para a avaliação fenotípica foram conduzidos sete experimentos em látice quadrado simples 14 x 14. A interação QTLs por locais foi expressiva, mas foram identificados alguns QTLs com estabilidade. Não houve concordância entre os resultados apresentados pelos dois métodos de mapeamento. Os marcadores que se mostraram mais estáveis e promissores para serem utilizados na seleção assistida para produtividade de grãos foram OPO-19 (1412 pb), OPO-20 (1585 pb) e OPN-07 (1445 pb).
650 $aFeijão
650 $aPhaseolus Vulgaris
650 $aProdução
653 $aAmbiente
653 $aInteração QTLs
653 $aMapeamento
653 $aMarcadores RAPD
700 1 $aSANTOS, J. B. dos
700 1 $aFERREIRA, D. F.
773 $tCrop Breeding and Applied Biotechnology$gv. 4, n. 2, p. 135-144, June 2004.
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Embrapa Arroz e Feijão (CNPAF) |
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