|
|
Registros recuperados : 13 | |
1. | | HISSA, D. C.; BEZERRA, G. A.; BIRNER-GRUENBERGER, R.; SILVA, L. P.; USÓN, I.; GRUBER, K.; MELO, V. M. M. Unique crystal structure of a novel surfactant protein from the foam nest of the frog Leptodactylus vastus. ChemBioChem, v. 15, p. 393-398, 2014. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
2. | | ARAUJO, D. M. F.; VASCONCELOS, S. J. T.; SILVA, M. R.; FONSECA, T. S.; MATTOS, M. C. F; LEMOS, T. L. G.; MELO, V. M. M. Emprego da levedura Pichia membranifaciens CE015 imobilizada em suporte de alginato de cálcio para redução da acetofenona. In: CONGRESSO BRASILEIRO DE QUÍMICA, 53., 2013, Natal. Química e sociedade: motores da sustentabilidade: anais. Natal: ABQ; UFRN, 2013. Biblioteca(s): Embrapa Amapá. |
| |
3. | | FARIAS, D. F.; ROSA, M. de F.; MELO, V. M. M.; ARAÚJO, A. J.; ROCHA-BEZERRA, L. C. B.; CARVALHO, A. F. U. Physicochemical and biological characterization of agrowaste from green coconut shell and its potential use in laboratory animal breeding. The Journal of Solid Waste Technology and Management, v. 38, n. 3, p. 194-201, 2012. Biblioteca(s): Embrapa Agroindústria Tropical. |
| |
4. | | FERNANDES, C. F.; MORAES, V. C. P.; MARTINS-MIRANDA A. S.; BARRETO, A. L. H.; CAPISTRANO, G. G. G.; VASCONCELOS, I. M.; MELO, V. M. M.; FREIRE FILHO, F. R.; KOGEL, K. -H. Cowpea x Collelotrichum lindemuthianum interaction: role of salieylie acid and lydrogen peroxide. In: REUNIÃO ANUAL: SOCIEDADE BRASILEIRA DE BIOQUÍMICA E BIOLOGIA MOLECULAR, 34., 2005, Águas de Lindóia. Programas e resumos... São Paulo: Sociedade Brasileira de Bioquímica e Biologia Molecular, 2005. Biblioteca(s): Embrapa Meio-Norte. |
| |
5. | | BRIGIDA, A. I. S.; BUARQUE, F. S.; NOGUEIRA, V. L. R.; MELO, V. M. M.; GUISÁN SEIJAS, J. M.; RIBEIRO, B. D.; GONÇALVES, L. R. B.; COELHO, M. A. Z. Partial purification of crude lipase extract from Yarrowia lipolytica: precipitation, aqueous two-phase systems (ATPS), and immobilization methods. Cleaner Chemical Engineering, v. 6, 11 p., art. 100105, June 2023. Biblioteca(s): Embrapa Agroindústria Tropical. |
| |
6. | | ROCHA, S. M. B.; MENDES, L. W.; OLIVEIRA, L. M. de S.; MELO, V. M. M.; ANTUNES, J. E. L.; ARAÚJO, F. F.; HUNGRIA, M.; ARAÚJO, A. S. F. Nodule microbiome from cowpea and lima bean grown in composted tannery sludge-treated soil Applied Soil Ecology, v. 151, 103542, 2020. 7 p. Biblioteca(s): Embrapa Soja. |
| |
7. | | BARRETO, A. L. H.; CAPISTRANO, G. G. G.; ELOY, Y. R. G.; MATOS, T. E.; MARTINS-MIRANDA, A. S.; FERNANDES, C. F.; FREIRE FILHO, F. R.; MELO, V. M. M.; VASCONCELOS, I. V.; OLIVEIRA, J. T. Peroxidase, Superoxide Dismutase, Catalase and Hydrogen Peroxide concentration in Cowpea [Vigna unguiculata (L.) Walp.], genotypes TE97 411-1E and BR3 Tracuatena, infected with Colletotrichum lindemuthianum. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUÍMICA E BIOLOGIA MOLECULAR, 33., 2004, Caxambú, MG. Programa e Resumos....Caxambú, MG: SBBq, 2004. Biblioteca(s): Embrapa Meio-Norte. |
| |
8. | | BARRETO, A. L. H.; VASCONCELOS, I. M.; GRANGEIRO; T. B.; MELO, V. M. M.; MATOS, T. E.; ELOY, Y. R. G.; FERNANDES, C. de F.; FREIRE-FILHO, F. R.; FREIRE, F. C. O.; OLIVEIRA, J. T. A. Infection process and host defense responses in compatible and incompatible interactions between cowpea (Vigna unguiculata) and Colletotrichum gloeosporioides. International Journal of Plant Science, v. 168, n.2, p. 193-203, 2007. Parceria: Embrapa Meio-Norte; Universidade Federal do Ceará e Embrapa Agroindústria Tropical. Biblioteca(s): Embrapa Rondônia. |
| |
9. | | BARRETO, A. L. H.; VASCONCELOS, I. M.; GRANGEIRO, T. B.; MELO, V. M. M.; MATOS, T. E.; ELOY, Y. R. G.; FERNANDES, C. F.; TORRES, D. C.; FREIRE FILHO, F. R.; FREIRE, F. C. O.; OLIVEIRA, J. T. A. Infection process and host defense responses in compatible and incompatible interactions between Cowpea (Vigna unguiculata) and Colletotrichum gloeosporioides. International Journal of Plant Sciences, Chicago, v. 168, n. 2, p. 193-203, 2007. Biblioteca(s): Embrapa Meio-Norte. |
| |
10. | | NOGUEIRA, V. L. R.; ROCHA, L. L.; COLARES, G. B.; ANGELIM, A. L.; NORMANDO, L. R. O.; CANTAO, M. E.; AGNEZ-LIMA, L. F.; ANDREOTE, F. D.; MELO, V. M. M. Microbiomes and potential metabolic pathways of pristine and anthropized Brazilian mangroves. Regional Studies in Marine Science, v. 2, p. 56-64, 2015. Biblioteca(s): Embrapa Suínos e Aves. |
| |
11. | | ALBUQUERQUE, T. M. de; MENDES, L. W.; ROCHA, S. M. B.; ANTUNES, J. E. L.; OLIVEIRA, L. M. de S.; MELO, V. M. M.; OLIVEIRA, F. A. S.; PEREIRA, A. P. de A.; SILVA, V. B. da; GOMES, R. L. F.; ALCANTARA NETO, F. de; LOPES, A. C. de A.; ROCHA, M. de M.; ARAUJO, A. S. F. Genetically related genotypes of cowpea present similar bacterial community in the rhizosphere. Scientific Reports, v. 12, 3472, 2022. Biblioteca(s): Embrapa Meio-Norte. |
| |
12. | | OLIVEIRA, J. T. A.; ANDRADE, N. C.; MIRANDA, A. S. M.; BARRETO, A. L. H.; MELO, V. M. M.; FERNANDES, C. F.; VASCONCELOS, I. M.; SILVEIRA, J. A. G.; CAVALCANTI, F. R.; FREIRE FILHO, F. R.; FREIRE, F. C. O.; GONÇALVES, F. J. T. Atividades peroxidásica e ß-1, 3 - Glucanásica elicitadas por agentes bióticos causadores de doenças e pelo estresse hídrico em feijão-de-corda (Vigna unguiculata (L.) Walp.). In: REUNIÃO NACIONAL DE PESQUISA DE CAUPI, 5., 2001, Teresina. Avanços tecnológicos no feijão caupi: anais. Teresina: Embrapa Meio-Norte, 2001. p. 19-23. (Embrapa Meio-Norte. Documentos, 56). Biblioteca(s): Embrapa Meio-Norte. |
| |
13. | | COSTA, R. M.; COSTA, M. K. L.; ROCHA, S. M. B.; LEITE, M. R. L.; ALCANTARA NETO, F. de; SOUZA, H. A. de; PEREIRA, A. P. de A.; MELO, V. M. M.; MEDEIROS, E. V. de; MENDES, L. W.; ARAUJO, A. S. F. Soil management shapes bacterial and archaeal communities in soybean rhizosphere: Comparison of no-tillage and integrated crop-livestock systems. Rhizosphere, v. 30, 100886, 2024. Biblioteca(s): Embrapa Meio-Norte. |
| |
Registros recuperados : 13 | |
|
|
Registro Completo
Biblioteca(s): |
Embrapa Agroindústria Tropical. |
Data corrente: |
12/01/2024 |
Data da última atualização: |
12/01/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BRIGIDA, A. I. S.; BUARQUE, F. S.; NOGUEIRA, V. L. R.; MELO, V. M. M.; GUISÁN SEIJAS, J. M.; RIBEIRO, B. D.; GONÇALVES, L. R. B.; COELHO, M. A. Z. |
Afiliação: |
ANA IRAIDY SANTA BRIGIDA, CNPAT; FILIPE SMITH BUARQUE, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; VANESSA LÚCIA RODRIGUES NOGUEIRA, UNIVERSIDADE FEDERAL DO CEARÁ; VÂNIA MARIA MACIEL MELO, UNIVERSIDADE FEDERAL DO CEARÁ; JOSÉ MANUEL GUISÁN SEIJAS, INSTITUTE OF CATALYSIS AND PETROCHEMISTRY; BERNARDO DIAS RIBEIRO, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; LUCIANA ROCHA BARROS GONÇALVES, UNIVERSIDADE FEDERAL DO CEARÁ; MARIA ALICE ZARUR COELHO, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO. |
Título: |
Partial purification of crude lipase extract from Yarrowia lipolytica: precipitation, aqueous two-phase systems (ATPS), and immobilization methods. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Cleaner Chemical Engineering, v. 6, 11 p., art. 100105, June 2023. |
DOI: |
10.1016/j.clce.2023.100105 |
Idioma: |
Inglês |
Conteúdo: |
Efforts have been concentrated on developing alternative methods of enzyme purification that are less costly and highly efficient. In this work, we evaluated three different methods for lipase purification from Yarrowia lipolytica, such as precipitation using ammonium sulfate, ethanol, or acetone; aqueous two-phase systems (ATPS) based on polyethylene glycol (PEG) and potassium phosphate; and direct immobilization. It was impossible to obtain stable precipitates of the enzyme due to the low concentration of total protein and the presence of biosurfactant produced by the microorganism. Different mixture compositions were selected for the partitioning study. Three ATPS showed selective partitioning of the target enzymes, i.e., lipase and protease migrated to opposite phases. In the ATPS composed of 13 wt% PEG-4000 and 10 wt% salts, it was possible to achieve a purification factor for lipase of 4.2. Purification by immobilization performed by lipase-lipase interactions showed three lipases of distinct sizes in the crude extract. In the immobilization method by hydrophobic supports, phenyl-agarose and butyl‑agarose were more selective in immobilizing than octyl-agarose. In the ion exchange immobilization method, only the lipases identified at 66 kDa and 41 kDa have an attraction for DEAE-agarose (anionic) and sulfopropyl-agarose (cationic) matrices. |
Palavras-Chave: |
Aqueous biphasic system; Aqueous two-phase system; Sistema aquoso bifásico; Sistema aquoso em duas fases. |
Thesaurus NAL: |
Chemical precipitation; Purification methods; Yarrowia lipolytica. |
Categoria do assunto: |
W Química e Física |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1160760/1/ART-23038.pdf
|
Marc: |
LEADER 02400naa a2200301 a 4500 001 2160760 005 2024-01-12 008 2023 bl uuuu u00u1 u #d 024 7 $a10.1016/j.clce.2023.100105$2DOI 100 1 $aBRIGIDA, A. I. S. 245 $aPartial purification of crude lipase extract from Yarrowia lipolytica$bprecipitation, aqueous two-phase systems (ATPS), and immobilization methods.$h[electronic resource] 260 $c2023 520 $aEfforts have been concentrated on developing alternative methods of enzyme purification that are less costly and highly efficient. In this work, we evaluated three different methods for lipase purification from Yarrowia lipolytica, such as precipitation using ammonium sulfate, ethanol, or acetone; aqueous two-phase systems (ATPS) based on polyethylene glycol (PEG) and potassium phosphate; and direct immobilization. It was impossible to obtain stable precipitates of the enzyme due to the low concentration of total protein and the presence of biosurfactant produced by the microorganism. Different mixture compositions were selected for the partitioning study. Three ATPS showed selective partitioning of the target enzymes, i.e., lipase and protease migrated to opposite phases. In the ATPS composed of 13 wt% PEG-4000 and 10 wt% salts, it was possible to achieve a purification factor for lipase of 4.2. Purification by immobilization performed by lipase-lipase interactions showed three lipases of distinct sizes in the crude extract. In the immobilization method by hydrophobic supports, phenyl-agarose and butyl‑agarose were more selective in immobilizing than octyl-agarose. In the ion exchange immobilization method, only the lipases identified at 66 kDa and 41 kDa have an attraction for DEAE-agarose (anionic) and sulfopropyl-agarose (cationic) matrices. 650 $aChemical precipitation 650 $aPurification methods 650 $aYarrowia lipolytica 653 $aAqueous biphasic system 653 $aAqueous two-phase system 653 $aSistema aquoso bifásico 653 $aSistema aquoso em duas fases 700 1 $aBUARQUE, F. S. 700 1 $aNOGUEIRA, V. L. R. 700 1 $aMELO, V. M. M. 700 1 $aGUISÁN SEIJAS, J. M. 700 1 $aRIBEIRO, B. D. 700 1 $aGONÇALVES, L. R. B. 700 1 $aCOELHO, M. A. Z. 773 $tCleaner Chemical Engineering$gv. 6, 11 p., art. 100105, June 2023.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Agroindústria Tropical (CNPAT) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|