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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
22/10/2015 |
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Data da última atualização: |
26/02/2016 |
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Autoria: |
CORDEIRO, M. A.; PEREIRA, N. N. de J.; BINOTI, D. H. B.; BINOTI, M. L. M. da S.; LEITE, H. G. |
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Título: |
Estimativa do volume de Acacia mangium utilizando técnicas de redes neurais artificiais e máquinas vetor de suporte. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Pesquisa Florestal Brasileira, Colombo, v. 35, n. 83, p. 255-261, jul./set. 2015. |
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DOI: |
10.4336/2015.pfb.35.83.596 |
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Idioma: |
Português |
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Conteúdo: |
Com o presente trabalho objetivou-se mostrar os resultados das estimativas volumétricas de Acacia mangium, obtidas pelo modelo de Schumacher e Hall, comparando-os com as metodologias de aplicação de redes neurais artificiais e máquinas vetor suporte. Para que fosse possível essa análise comparativa, foram utilizados dados de cubagens de 31 árvores de povoamentos de Acacia mangium, localizados no norte do estado do Amapá. Os dados apresentavam idade variando de 14 a 17 anos. As árvores-amostra foram cubadas em seções relativas, realizando medições de diâmetros e espessuras das cascas ao longo do fuste em 14 seções, baseando-se nos seguintes percentuais das alturas totais: 0,05%, 1%, 5%, 10%, 15%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% e 95%, sendo o volume total com casca obtido pela aplicação da fórmula de Smalian. De modo geral, as metodologias que diferem da tradicional apresentaram resultados estatisticamente superiores. |
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Palavras-Chave: |
Estimativa volumétrica; Modelagem; Modeling; Smalian; Volumetric estimates. |
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Thesagro: |
Madeira; Volume. |
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Thesaurus Nal: |
Acacia mangium. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/132791/1/EstimativadoVolume.pdf
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Marc: |
LEADER 01820naa a2200277 a 4500
001 2027059
005 2016-02-26
008 2015 bl uuuu u00u1 u #d
024 7 $a10.4336/2015.pfb.35.83.596$2DOI
100 1 $aCORDEIRO, M. A.
245 $aEstimativa do volume de Acacia mangium utilizando técnicas de redes neurais artificiais e máquinas vetor de suporte.$h[electronic resource]
260 $c2015
520 $aCom o presente trabalho objetivou-se mostrar os resultados das estimativas volumétricas de Acacia mangium, obtidas pelo modelo de Schumacher e Hall, comparando-os com as metodologias de aplicação de redes neurais artificiais e máquinas vetor suporte. Para que fosse possível essa análise comparativa, foram utilizados dados de cubagens de 31 árvores de povoamentos de Acacia mangium, localizados no norte do estado do Amapá. Os dados apresentavam idade variando de 14 a 17 anos. As árvores-amostra foram cubadas em seções relativas, realizando medições de diâmetros e espessuras das cascas ao longo do fuste em 14 seções, baseando-se nos seguintes percentuais das alturas totais: 0,05%, 1%, 5%, 10%, 15%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% e 95%, sendo o volume total com casca obtido pela aplicação da fórmula de Smalian. De modo geral, as metodologias que diferem da tradicional apresentaram resultados estatisticamente superiores.
650 $aAcacia mangium
650 $aMadeira
650 $aVolume
653 $aEstimativa volumétrica
653 $aModelagem
653 $aModeling
653 $aSmalian
653 $aVolumetric estimates
700 1 $aPEREIRA, N. N. de J.
700 1 $aBINOTI, D. H. B.
700 1 $aBINOTI, M. L. M. da S.
700 1 $aLEITE, H. G.
773 $tPesquisa Florestal Brasileira, Colombo$gv. 35, n. 83, p. 255-261, jul./set. 2015.
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Registro original: |
Embrapa Florestas (CNPF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
14/01/2020 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
FREITAS, V. J. F.; CAMPELO, I. S.; SILVA, M. M. A. S.; CAVALCANTI, C. M.; TEIXEIRA, D. I. A.; CAMARGO, L. S. de A.; MELO L. M.; RÁDIS-BAPTISTA, G. |
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Afiliação: |
Vicente J. F. Freitas; Iana S. Campelo; Mirelly M .A. S. Silva; Camila M. Cavalcanti; Dárcio I. A. Teixeira; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; Luciana M. Melo; Gandhi Rádis-Baptista. |
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Título: |
Disulphide-less crotamine is effective for formation of DNA-peptide complex but is unable to improve bovine embryo transfection. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Zygote, v. 28, n. 1, p. 72-79, 2020. |
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DOI: |
https://doi.org/10.1017/s0967199419000716 |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to investigate the ability of disulphide-less crotamine (dLCr) to complex DNA and to evaluate whether the DNA-dLCr complex is capable of improving transfection in bovine embryos. Three experiments were performed to: (i) evaluate the formation and stability of the DNA-dLCr complex; (ii) assess the dLCr embryotoxicity by exposure of bovine embryos to dLCr; and (iii) assess the efficiency of bovine embryo transfection after microinjection of the DNA-dLCr complex or green fluorescent protein (GFP) plasmid alone (control). DNA complexation by dLCr after 30 min of incubation at 1:100 and 1:50 proportions presented higher efficiency (P < 0.05) than the two controls: native crotamine (NCr) 1:10 and lipofectamine. There was no difference between DNA-dLCr 1:25 and the controls. The DNA-dLCr complexation was evaluated at different proportions and times. In all, at least half of maximum complexation was achieved within the initial 30 min. No embryotoxicity of dLCr was verified after exposure of in vitro fertilized embryos to different concentrations of the peptide. The effectiveness of dLCr to improve exogenous gene expression was evaluated by microinjection of the DNA-dLCr complex into in vitro fertilized zygotes, followed by verification of both embryo development and GFP expression. From embryos microinjected with DNA only, 4.6% and 2.8% expressed the GFP transgene at day 5 and day 7, respectively. The DNA-dLCr complex did not increase the number of GFP-positive embryos. In conclusion, dLCr forms a complex with DNA and its application in in vitro culture is possible. However, the dLCr peptide sequence should be redesigned to improve GFP expression. MenosThis study aimed to investigate the ability of disulphide-less crotamine (dLCr) to complex DNA and to evaluate whether the DNA-dLCr complex is capable of improving transfection in bovine embryos. Three experiments were performed to: (i) evaluate the formation and stability of the DNA-dLCr complex; (ii) assess the dLCr embryotoxicity by exposure of bovine embryos to dLCr; and (iii) assess the efficiency of bovine embryo transfection after microinjection of the DNA-dLCr complex or green fluorescent protein (GFP) plasmid alone (control). DNA complexation by dLCr after 30 min of incubation at 1:100 and 1:50 proportions presented higher efficiency (P < 0.05) than the two controls: native crotamine (NCr) 1:10 and lipofectamine. There was no difference between DNA-dLCr 1:25 and the controls. The DNA-dLCr complexation was evaluated at different proportions and times. In all, at least half of maximum complexation was achieved within the initial 30 min. No embryotoxicity of dLCr was verified after exposure of in vitro fertilized embryos to different concentrations of the peptide. The effectiveness of dLCr to improve exogenous gene expression was evaluated by microinjection of the DNA-dLCr complex into in vitro fertilized zygotes, followed by verification of both embryo development and GFP expression. From embryos microinjected with DNA only, 4.6% and 2.8% expressed the GFP transgene at day 5 and day 7, respectively. The DNA-dLCr complex did not increase the number of GFP-positive embr... Mostrar Tudo |
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Palavras-Chave: |
Gene delivery; Microinjection; Nucleic acid-peptide complex. |
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Thesaurus NAL: |
Embryotoxicity; Transgenesis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02565naa a2200277 a 4500
001 2118715
005 2024-02-06
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1017/s0967199419000716$2DOI
100 1 $aFREITAS, V. J. F.
245 $aDisulphide-less crotamine is effective for formation of DNA-peptide complex but is unable to improve bovine embryo transfection.$h[electronic resource]
260 $c2020
520 $aThis study aimed to investigate the ability of disulphide-less crotamine (dLCr) to complex DNA and to evaluate whether the DNA-dLCr complex is capable of improving transfection in bovine embryos. Three experiments were performed to: (i) evaluate the formation and stability of the DNA-dLCr complex; (ii) assess the dLCr embryotoxicity by exposure of bovine embryos to dLCr; and (iii) assess the efficiency of bovine embryo transfection after microinjection of the DNA-dLCr complex or green fluorescent protein (GFP) plasmid alone (control). DNA complexation by dLCr after 30 min of incubation at 1:100 and 1:50 proportions presented higher efficiency (P < 0.05) than the two controls: native crotamine (NCr) 1:10 and lipofectamine. There was no difference between DNA-dLCr 1:25 and the controls. The DNA-dLCr complexation was evaluated at different proportions and times. In all, at least half of maximum complexation was achieved within the initial 30 min. No embryotoxicity of dLCr was verified after exposure of in vitro fertilized embryos to different concentrations of the peptide. The effectiveness of dLCr to improve exogenous gene expression was evaluated by microinjection of the DNA-dLCr complex into in vitro fertilized zygotes, followed by verification of both embryo development and GFP expression. From embryos microinjected with DNA only, 4.6% and 2.8% expressed the GFP transgene at day 5 and day 7, respectively. The DNA-dLCr complex did not increase the number of GFP-positive embryos. In conclusion, dLCr forms a complex with DNA and its application in in vitro culture is possible. However, the dLCr peptide sequence should be redesigned to improve GFP expression.
650 $aEmbryotoxicity
650 $aTransgenesis
653 $aGene delivery
653 $aMicroinjection
653 $aNucleic acid-peptide complex
700 1 $aCAMPELO, I. S.
700 1 $aSILVA, M. M. A. S.
700 1 $aCAVALCANTI, C. M.
700 1 $aTEIXEIRA, D. I. A.
700 1 $aCAMARGO, L. S. de A.
700 1 $aMELO L. M.
700 1 $aRÁDIS-BAPTISTA, G.
773 $tZygote$gv. 28, n. 1, p. 72-79, 2020.
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