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| 163. |  | BONFIM, K.; LACORTE, C.; ARAGÃO, F. J. L.; OLIVEIRA, S. C.; RECH, E. L. Introdução e expressão do gene Lack do antígeno da Leishmaniose em alface (Lactuca Sativa). In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 7., 2002, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2002. p. 51.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 167. | | PROSDOCIMI, F.; BITTENCOURT, D.; SILVA, F. R. D.; MOTTA, P. C.; RECH, E. Molecular, behavioral and anatomical sophistication in spider webs: insights from spinning gland RNA-seq experiments in primitive and modern spiders. In: INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 6., 2010, Ouro Preto. Abstracts... [S.l.: s.n.], 2010. p. 194. p. 67. X-Meeting 2010.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 173. | | CUNHA, N. B. da; MURAD, A. M.; VIANNA, G. R.; RECH FILHO, E. L. Recombinant biosynthesis of functional human growth hormone and coagulation factor IX in transgenic soybean seeds. BMC Proceedings, v.8, suppl, P.112, 2014. (Open access) Edição do Congress of the Brazilian Biotechnology Society, Florianópolis, 2013.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 176. | | CASA, J.; BOFF, M. I. C.; RECH, T. D.; BOFF, P. Resistência do vimeiro, Salix spp. (Salicaceae), a pragas e doenças. Ciência Florestal, Santa Maria, v. 17, n. 1/2, p. 1-8, mar./jun. 2007.| Biblioteca(s): Embrapa Florestas. |
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| 177. | | RECH, T. D.; ZANETTE, F.; BRANDES, D.; AMARANTE, C.; CABEL, S. R. Requerimento em frio, dinâmica e heterogeneidade de dormência de gemas em ramos de Salix x rubens cultivado em Lages, SC. Ciência Florestal, Santa Maria, v. 16, n. 3/4, p. 427-435, set. 2006.| Biblioteca(s): Embrapa Florestas. |
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| 180. | | OKINO, C. H.; RECH, R. R.; TREVISOL, I. M.; JAENISCH, F. R. F.; BRENTANO, L. Por que o diagnóstico de Bronquite infecciosa é importante? Avicultura Industrial, Itu, ed. 1219, ano 104, n. 2, p. 16-25, 2013.| Biblioteca(s): Embrapa Suínos e Aves. |
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| Registros recuperados : 472 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
04/01/1994 |
|
Data da última atualização: |
30/07/2021 |
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Autoria: |
MELO, I. S. de; HEALE, J. B. |
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Afiliação: |
ITAMAR SOARES DE MELO, CNPMA. |
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Título: |
Isolation and regeneration of protoplasts from Trichoderma pseudokoningii: a biocontrol agent of plant pathogens. |
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Ano de publicação: |
1989 |
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Fonte/Imprenta: |
Summa Phytopathologica, v.15, n.1, p.34, jan./mar. 1989. |
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Idioma: |
Inglês |
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Conteúdo: |
T. pseudokoningii has controlled naturally "dry eye rot" lesions caused by Botrytis cinerea on fallen apple fruit, Rhizoctonia solani and Sclerotinia minor in filamentous fungi normally requires the production on protoplasts. Mycelium of Trichoderma was incubated with a number of different lytic enzymes and stabilizers to determine their effectiveness in releasing protoplasts. The effect of culture age was also investigated. The enzyme novozym 234, alone and in combination with celulase from T. viride (BHD product n. 39074) was successful in releasing protoplasts. Inorganic salts containing 0.6M (NH4)2SO4 were most effective as osmotic stabilizer. Culture age was important in obtaining high protoplasts yield: pre - log phase cultures gave the highest yields.Protoplasts were capable of regeneration on solid media incorporating an osmotic stabilizer. Regeneration frequencies of about 11% were obtained. Observations on regenerating protoplasts showed that protoplasts either produced a germ tube directly, or did so after a period growth by budding. Nuclear staining with Giemsa showed that many protoplasts were multinucleate. |
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Palavras-Chave: |
Antagonism; Biocontrol; Disease; Isolation; Plant; Protoplast; Regeneration. |
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Thesagro: |
Botrytis Cinerea; Rhizoctonia Solani; Sclerotinia Minor; Trichoderma Viride. |
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Thesaurus NAL: |
biological control; fungi; Trichoderma pseudokoningii. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01980naa a2200301 a 4500
001 1011107
005 2021-07-30
008 1989 bl uuuu u00u1 u #d
100 1 $aMELO, I. S. de
245 $aIsolation and regeneration of protoplasts from Trichoderma pseudokoningii$ba biocontrol agent of plant pathogens.
260 $c1989
520 $aT. pseudokoningii has controlled naturally "dry eye rot" lesions caused by Botrytis cinerea on fallen apple fruit, Rhizoctonia solani and Sclerotinia minor in filamentous fungi normally requires the production on protoplasts. Mycelium of Trichoderma was incubated with a number of different lytic enzymes and stabilizers to determine their effectiveness in releasing protoplasts. The effect of culture age was also investigated. The enzyme novozym 234, alone and in combination with celulase from T. viride (BHD product n. 39074) was successful in releasing protoplasts. Inorganic salts containing 0.6M (NH4)2SO4 were most effective as osmotic stabilizer. Culture age was important in obtaining high protoplasts yield: pre - log phase cultures gave the highest yields.Protoplasts were capable of regeneration on solid media incorporating an osmotic stabilizer. Regeneration frequencies of about 11% were obtained. Observations on regenerating protoplasts showed that protoplasts either produced a germ tube directly, or did so after a period growth by budding. Nuclear staining with Giemsa showed that many protoplasts were multinucleate.
650 $abiological control
650 $afungi
650 $aTrichoderma pseudokoningii
650 $aBotrytis Cinerea
650 $aRhizoctonia Solani
650 $aSclerotinia Minor
650 $aTrichoderma Viride
653 $aAntagonism
653 $aBiocontrol
653 $aDisease
653 $aIsolation
653 $aPlant
653 $aProtoplast
653 $aRegeneration
700 1 $aHEALE, J. B.
773 $tSumma Phytopathologica$gv.15, n.1, p.34, jan./mar. 1989.
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