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Registro Completo
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
02/02/2018 |
Data da última atualização: |
09/03/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
C - 0 |
Autoria: |
SANTOS, S. N.; MELO, I. S. de. |
Afiliação: |
SUIKINAI NOBRE SANTOS, FAPESP; ITAMAR SOARES DE MELO, CNPMA. |
Título: |
A rapid primary screening method for antitumor using the oomycete Pythium aphanidermatum. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Natural Products Chemistry & Research, v. 4, n. 6, 2016. |
DOI: |
10.4172/2329-6836.1000241 |
Idioma: |
Inglês |
Conteúdo: |
Investigating bioactive components from microbial metabolites can provide potential source for drug discovery. There are a number of traditional antitumor compounds screening assays already described. In this study, we have developed an efficient method to search for antitumor compounds using the Oomycete Pythium aphanidermatum as model on agar plates for high throughtput screening. Antioomycete compounds showed cytotoxic effects in human cancer cells lines due to the possible similarity between the lipid cell membrane of the Pythium species and cancerous cells, suggesting the compounds act through similar mechanisms of inhibition. In this assay, i t is possible to evaluate hundreds of samples, with results in few hours. The Pythium assay was shown to be sensitive for several chemical substances and allowed the detection and isolation of antitumor compounds from microorganisms. This bioassay was shown to be sensitive, rapid, simple and reliable for antitumor activity. It takes just few hours to evaluate the properties of the extracts. |
Palavras-Chave: |
Antifungal assay; Antitumoral screening. |
Thesagro: |
Pythium Aphanidermatum. |
Thesaurus NAL: |
Antineoplastic agents; Screening; secondary metabolites. |
Categoria do assunto: |
S Ciências Biológicas |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/172110/1/2017AP61.pdf
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Marc: |
LEADER 01732naa a2200217 a 4500 001 2087004 005 2018-03-09 008 2016 bl uuuu u00u1 u #d 024 7 $a10.4172/2329-6836.1000241$2DOI 100 1 $aSANTOS, S. N. 245 $aA rapid primary screening method for antitumor using the oomycete Pythium aphanidermatum.$h[electronic resource] 260 $c2016 520 $aInvestigating bioactive components from microbial metabolites can provide potential source for drug discovery. There are a number of traditional antitumor compounds screening assays already described. In this study, we have developed an efficient method to search for antitumor compounds using the Oomycete Pythium aphanidermatum as model on agar plates for high throughtput screening. Antioomycete compounds showed cytotoxic effects in human cancer cells lines due to the possible similarity between the lipid cell membrane of the Pythium species and cancerous cells, suggesting the compounds act through similar mechanisms of inhibition. In this assay, i t is possible to evaluate hundreds of samples, with results in few hours. The Pythium assay was shown to be sensitive for several chemical substances and allowed the detection and isolation of antitumor compounds from microorganisms. This bioassay was shown to be sensitive, rapid, simple and reliable for antitumor activity. It takes just few hours to evaluate the properties of the extracts. 650 $aAntineoplastic agents 650 $aScreening 650 $asecondary metabolites 650 $aPythium Aphanidermatum 653 $aAntifungal assay 653 $aAntitumoral screening 700 1 $aMELO, I. S. de 773 $tNatural Products Chemistry & Research$gv. 4, n. 6, 2016.
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Embrapa Meio Ambiente (CNPMA) |
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