Data/hora: 17/06/2024 11:10:22

Biblioteca(s): | Embrapa Meio Ambiente.
Data corrente: | 04/01/1994
Data da última atualização: | 30/07/2021
Autoria: | MELO, I. S. de; HEALE, J. B.
Afiliação: | ITAMAR SOARES DE MELO, CNPMA.
Título: | Isolation and regeneration of protoplasts from Trichoderma pseudokoningii: a biocontrol agent of plant pathogens.
Ano de publicação: | 1989
Fonte/Imprenta: | Summa Phytopathologica, v.15, n.1, p.34, jan./mar. 1989.
Idioma: | Inglês
Conteúdo: | T. pseudokoningii has controlled naturally "dry eye rot" lesions caused by Botrytis cinerea on fallen apple fruit, Rhizoctonia solani and Sclerotinia minor in filamentous fungi normally requires the production on protoplasts. Mycelium of Trichoderma was incubated with a number of different lytic enzymes and stabilizers to determine their effectiveness in releasing protoplasts. The effect of culture age was also investigated. The enzyme novozym 234, alone and in combination with celulase from T. viride (BHD product n. 39074) was successful in releasing protoplasts. Inorganic salts containing 0.6M (NH4)2SO4 were most effective as osmotic stabilizer. Culture age was important in obtaining high protoplasts yield: pre - log phase cultures gave the highest yields.Protoplasts were capable of regeneration on solid media incorporating an osmotic stabilizer. Regeneration frequencies of about 11% were obtained. Observations on regenerating protoplasts showed that protoplasts either produced a germ tube directly, or did so after a period growth by budding. Nuclear staining with Giemsa showed that many protoplasts were multinucleate.
Palavras-Chave: | Antagonism; Biocontrol; Disease; Isolation; Plant; Protoplast; Regeneration.
Thesagro: | Botrytis Cinerea; Rhizoctonia Solani; Sclerotinia Minor; Trichoderma Viride.
Thesaurus Nal: | biological control; fungi; Trichoderma pseudokoningii.
Categoria do assunto: | --
Registro original: | Embrapa Meio Ambiente (CNPMA)
Exemplares: | CNPMA - (1740-1) - UPC - AP - -- -  -  -  - DIS