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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
02/10/2013 |
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Data da última atualização: |
02/10/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
PETTA, T.; RAICHARDT, L.; MELO, I. S. de; MORAES, L. A. B. |
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Afiliação: |
TÂNIA PETTA, FFCLRP-USP; LEANDRO RAICHARDT, FFCLRP-USP; ITAMAR SOARES DE MELO, CNPMA; LUIZ A. B. MORAES, FFCLRP-USP. |
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Título: |
Bioassay-guided isolation of a low molecular weight PHB from Burkholderia sp. with phytotoxic activity. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Applied Biochemistry and Biotechnology, Totowa, v. 170, n. 7, p. 1689-1701, 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
This work reports on the bioassay-guided isolation and identification of themacrocyclic pentolide 1, a cyclic polyhydroxybutyrate (PHB) with low molecularweight. This metabolite is produced by Burkholderia sp. and it exhibited phytotoxicactivity in a Lemna minor bioassay. Its structure was determined by 1H and 13CNMR, heteronuclear multiple quantum correlation, heteronuclear multiple bond correlation, IR, and electrospray ionization tandem mass spectrometry analyses. The period for maximum production of the pentolide was optimized and determined on the basis of multiple reaction monitoring experiments at 15 days. The potential of Burkholderia sp. as a producer of higher biopolymers of PHB was also investigated. The methodology employed here accelerated the isolation and characterization of a phytotoxicmetabolite whose structure can serve as a model for the synthesis of new classes of herbicides. |
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Palavras-Chave: |
Bioassay; Burkholderia sp; LC-MS; Liquid chromatography-mass spectrometry; Polihidroxibutirato; Polímero. |
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Thesagro: |
Bactéria; Herbicida; Toxidez. |
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Thesaurus NAL: |
Lemna minor; phytotoxicity; polyhydroxybutyrate. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 01796naa a2200301 a 4500
001 1967673
005 2013-10-02
008 2013 bl uuuu u00u1 u #d
100 1 $aPETTA, T.
245 $aBioassay-guided isolation of a low molecular weight PHB from Burkholderia sp. with phytotoxic activity.$h[electronic resource]
260 $c2013
520 $aThis work reports on the bioassay-guided isolation and identification of themacrocyclic pentolide 1, a cyclic polyhydroxybutyrate (PHB) with low molecularweight. This metabolite is produced by Burkholderia sp. and it exhibited phytotoxicactivity in a Lemna minor bioassay. Its structure was determined by 1H and 13CNMR, heteronuclear multiple quantum correlation, heteronuclear multiple bond correlation, IR, and electrospray ionization tandem mass spectrometry analyses. The period for maximum production of the pentolide was optimized and determined on the basis of multiple reaction monitoring experiments at 15 days. The potential of Burkholderia sp. as a producer of higher biopolymers of PHB was also investigated. The methodology employed here accelerated the isolation and characterization of a phytotoxicmetabolite whose structure can serve as a model for the synthesis of new classes of herbicides.
650 $aLemna minor
650 $aphytotoxicity
650 $apolyhydroxybutyrate
650 $aBactéria
650 $aHerbicida
650 $aToxidez
653 $aBioassay
653 $aBurkholderia sp
653 $aLC-MS
653 $aLiquid chromatography-mass spectrometry
653 $aPolihidroxibutirato
653 $aPolímero
700 1 $aRAICHARDT, L.
700 1 $aMELO, I. S. de
700 1 $aMORAES, L. A. B.
773 $tApplied Biochemistry and Biotechnology, Totowa$gv. 170, n. 7, p. 1689-1701, 2013.
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