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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
06/01/2014 |
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Data da última atualização: |
08/01/2014 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
RAFAEL, H. A.; SOUZA, I. R. P. de; BARROS, B. A.; PINTO, M. O.; MOREIRA, R. O.; GONÇALVES, I. A. M.; CARVALHO, S. G. M. |
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Afiliação: |
ISABEL REGINA PRAZERES DE SOUZA, CNPMS; BEATRIZ DE ALMEIDA BARROS, CNPMS; MARCOS DE OLIVEIRA PINTO, CNPMS. |
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Título: |
Molecular detection of the Sugarcane Mosaic Virus causing mosaic disease in maize and sorghum in Brazil. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE GENÉTICA, 59., 2013, Águas de Lindóia. Resumos... Ribeirão Preto: SBG, 2013. p. 94. |
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Idioma: |
Inglês |
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Thesagro: |
Doença de planta; Milho; Mosaico; Sorgo; Vírus. |
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Thesaurus Nal: |
Plant breeding; Plant diseases and disorders. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/94737/1/Molecular-detection.pdf
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Marc: |
LEADER 00832nam a2200253 a 4500
001 1975005
005 2014-01-08
008 2013 bl uuuu u00u1 u #d
100 1 $aRAFAEL, H. A.
245 $aMolecular detection of the Sugarcane Mosaic Virus causing mosaic disease in maize and sorghum in Brazil.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE GENÉTICA, 59., 2013, Águas de Lindóia. Resumos... Ribeirão Preto: SBG, 2013. p. 94.$c2013
650 $aPlant breeding
650 $aPlant diseases and disorders
650 $aDoença de planta
650 $aMilho
650 $aMosaico
650 $aSorgo
650 $aVírus
700 1 $aSOUZA, I. R. P. de
700 1 $aBARROS, B. A.
700 1 $aPINTO, M. O.
700 1 $aMOREIRA, R. O.
700 1 $aGONÇALVES, I. A. M.
700 1 $aCARVALHO, S. G. M.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
05/02/2024 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 4 |
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Autoria: |
DUARTE, M. F.; ANDRADE, I. A. de; SILVA, J. M. F.; MELO, F. L. de; MACHADO, A. M.; INOUE-NAGATA, A. K.; NAGATA, T. |
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Afiliação: |
MACÁRIA FERREIRA DUARTE, UNIVERSIDADE DE BRASÍLIA; IKARO ALVES DE ANDRADE, UNIVERSIDADE DE BRASÍLIA; JOÃO MARCOS FAGUNDES SILVA, UNIVERSIDADE DE BRASÍLIA; FERNANDO LUCAS DE MELO, UNIVERSIDADE DE BRASILIA; ANA MARIA MACHADO, COMPANHIA DE SANEAMENTO AMBIENTAL DO DF; ALICE KAZUKO INOUE NAGATA, CNPH; TATSUYA NAGATA, UNIVERSIDADE DE BRASÍLIA. |
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Título: |
Metagenomic analyses of plant virus sequences in sewage water for plant viruses monitoring. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Tropical Plant Pathology, v. 48, p. 408-416, 2023. |
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DOI: |
10.1007/s40858-023-00575-8 |
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Idioma: |
Inglês |
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Conteúdo: |
Frequent monitoring of emerging viruses of agricultural crops is one of the most important missions for plant virologists. A fast and precise identifcation of potential harmful viruses may prevent the occurrence of serious epidemics. Nowadays, highthroughput sequencing (HTS) technologies became an accessible and powerful tool for this purpose. The major discussion of this strategy resides in the process of sample collection, which is usually laborious, costly and nonrepresentative. In this study, we assessed the use of sewage water samples for monitoring the widespread, numerous, and stable plant viruses using HTS analysis and RT-qPCR. Plant viruses belonged to 12 virus families were found, from which Virgaviridae, Solemoviridae, Tymoviridae, Alphafexiviridae, Betafexiviridae, losteroviridae and Secoviridae were the most abundant ones with more than 20 species. Additionally, we detected one quarantine virus in Brazil and a new tobamovirus species. To assess the importance of the processed foods as virus release origins to sewage, we selected two viruses, the tobamovirus pepper mild mottle virus (PMMoV) and the carlavirus garlic common latent virus (GarCLV), to detect in processed food materials by RT-qPCR. PMMoV was detected in large amount in pepper-based processed foods and in sewage samples, while GarCLV was less frequent in dried and fresh garlic samples, and in the sewage samples. This suggested a high correlation of virus abundance in sewage and processed food sources. The potential use of sewage for a virus survey is discussed in this study. MenosFrequent monitoring of emerging viruses of agricultural crops is one of the most important missions for plant virologists. A fast and precise identifcation of potential harmful viruses may prevent the occurrence of serious epidemics. Nowadays, highthroughput sequencing (HTS) technologies became an accessible and powerful tool for this purpose. The major discussion of this strategy resides in the process of sample collection, which is usually laborious, costly and nonrepresentative. In this study, we assessed the use of sewage water samples for monitoring the widespread, numerous, and stable plant viruses using HTS analysis and RT-qPCR. Plant viruses belonged to 12 virus families were found, from which Virgaviridae, Solemoviridae, Tymoviridae, Alphafexiviridae, Betafexiviridae, losteroviridae and Secoviridae were the most abundant ones with more than 20 species. Additionally, we detected one quarantine virus in Brazil and a new tobamovirus species. To assess the importance of the processed foods as virus release origins to sewage, we selected two viruses, the tobamovirus pepper mild mottle virus (PMMoV) and the carlavirus garlic common latent virus (GarCLV), to detect in processed food materials by RT-qPCR. PMMoV was detected in large amount in pepper-based processed foods and in sewage samples, while GarCLV was less frequent in dried and fresh garlic samples, and in the sewage samples. This suggested a high correlation of virus abundance in sewage and processed food sources.... Mostrar Tudo |
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Palavras-Chave: |
High-throughput sequencing. |
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Thesagro: |
Vírus. |
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Thesaurus NAL: |
Wastewater. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02294naa a2200241 a 4500
001 2161643
005 2024-02-05
008 2023 bl uuuu u00u1 u #d
024 7 $a10.1007/s40858-023-00575-8$2DOI
100 1 $aDUARTE, M. F.
245 $aMetagenomic analyses of plant virus sequences in sewage water for plant viruses monitoring.$h[electronic resource]
260 $c2023
520 $aFrequent monitoring of emerging viruses of agricultural crops is one of the most important missions for plant virologists. A fast and precise identifcation of potential harmful viruses may prevent the occurrence of serious epidemics. Nowadays, highthroughput sequencing (HTS) technologies became an accessible and powerful tool for this purpose. The major discussion of this strategy resides in the process of sample collection, which is usually laborious, costly and nonrepresentative. In this study, we assessed the use of sewage water samples for monitoring the widespread, numerous, and stable plant viruses using HTS analysis and RT-qPCR. Plant viruses belonged to 12 virus families were found, from which Virgaviridae, Solemoviridae, Tymoviridae, Alphafexiviridae, Betafexiviridae, losteroviridae and Secoviridae were the most abundant ones with more than 20 species. Additionally, we detected one quarantine virus in Brazil and a new tobamovirus species. To assess the importance of the processed foods as virus release origins to sewage, we selected two viruses, the tobamovirus pepper mild mottle virus (PMMoV) and the carlavirus garlic common latent virus (GarCLV), to detect in processed food materials by RT-qPCR. PMMoV was detected in large amount in pepper-based processed foods and in sewage samples, while GarCLV was less frequent in dried and fresh garlic samples, and in the sewage samples. This suggested a high correlation of virus abundance in sewage and processed food sources. The potential use of sewage for a virus survey is discussed in this study.
650 $aWastewater
650 $aVírus
653 $aHigh-throughput sequencing
700 1 $aANDRADE, I. A. de
700 1 $aSILVA, J. M. F.
700 1 $aMELO, F. L. de
700 1 $aMACHADO, A. M.
700 1 $aINOUE-NAGATA, A. K.
700 1 $aNAGATA, T.
773 $tTropical Plant Pathology$gv. 48, p. 408-416, 2023.
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