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| Registros recuperados : 63 | |
| 4. |  | FERREIRA, B. C.; MELO, F. L.; RIBEIRO, B. M.; SOUZA, M. L. de. Sequenciamento do genoma de dois clones do baculovírus Anticarsia gemmatalis MNPV com variação de virulência. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 13., 2013, Bonito, MS. Faça bonito: use controle biológico: anais. Brasília, DF: Embrapa, 2013.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 5. | | ALVES-FREITAS, D. M. T.; MELO, F. L.; FARIA, J. C.; RIBEIRO, S. G. DSRNA deep sequencing reveals five viral species in common beans. Virus Reviews & Research, Brasília, DF, v. 20, n. 2, p. 43-44, Aug./Dec. 2016. Edição dos Resumos do XXVII Brazilian Congress of Virology, XI Mercosur Meeting of Virology, Pirenópolis, GO, Sept. 2016. Ref. PIV99.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | ALVES-FREITAS, D. M. T.; MELO, F. L.; FARIA, J. C.; RIBEIRO, S. G. DSRNA deep sequencing reveals five viral species in common beans. Virus Reviews & Research, Brasília, DF, v. 20, n. 2, p. 43-44, Aug./Dec. 2016. Ref. PIV99. Edição dos Resumos do XXVII Brazilian Congress of Virology, XI Mercosur Meeting of Virology, Pirenópolis, GO, Sept. 2016.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 10. | | OLIVEIRA, A. S. de; MELO, F. L.; INOUE NAGATA, A. K.; NAGATA, T.; KITAJIMA, E. W.; RESENDE, R. O. Characterization of bean necrotic mosaic virus: a member of a novel evolutionary lineage within the genus Tospovirus. Plos One, San Francisco, v. 7, n. 6, p. 1-9, June 2012.| Biblioteca(s): Embrapa Hortaliças. |
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| 11. | | LAMAS, N. S.; FONTENELE, R. S.; MELO, F. L.; COSTA, A. F.; VARSANI, A.; RIBEIRO, S. G. Complete genome sequence of a genomovirus associated with common bean plant leaves in Brazil. Genome Announcements, v. 4, n. 6, e01247-16, 2016.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | SOSA-GÓMEZ, D. R.; RIBEIRO, B. M.; ARDISSON-ARAÚJO, D. M. P.; MELO, F. L.; CARVALHO, R. A.; MARTINELLI, S. Atividade biológica de toxinas e de um isolado de Baculovírus (SNPV) caracterizado molecularmente, em Helicoverpa armigera e Helicoverpa zea. In: REUNIÃO DE PESQUISA DE SOJA, 34., 2014, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2014. p. 71-72.| Biblioteca(s): Embrapa Soja. |
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| 19. | | LIMA, B. P. de; ALVES-FREITAS, D. M. T.; MELO, F. L. de; CARVALHO, R. de C. P.; FARIA, J. C. de; RIBEIRO, S. G. Characterization of a new whitefly-transmitted (Bemisia tabaci Meam 1) cytorhabdovirus infecting common bean. In: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 12., 2017, Piracicaba. Produtividade e sustentabilidade da cultura do feijão: do campo para a mesa: resumos. Piracicaba: CENA: IAC, 2017. p. 52. CONAFE| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 20. | | LIMA, B. P. de; ALVES-FREITAS, D. M. T.; MELO, F. L. de; CARVALHO, R. de C. P.; FARIA, J. C. de; RIBEIRO, S. G. Characterization of a new whitefly-transmitted (Bemisia tabaci Meam 1) cytorhabdovirus infecting common bean. In: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 12., 2017, Piracicaba. Produtividade e sustentabilidade da cultura do feijão: do campo para a mesa: resumos. Piracicaba: CENA: IAC, 2017. p. 52. CONAFE| Biblioteca(s): Embrapa Arroz e Feijão. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
05/02/2024 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 4 |
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Autoria: |
DUARTE, M. F.; ANDRADE, I. A. de; SILVA, J. M. F.; MELO, F. L. de; MACHADO, A. M.; INOUE-NAGATA, A. K.; NAGATA, T. |
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Afiliação: |
MACÁRIA FERREIRA DUARTE, UNIVERSIDADE DE BRASÍLIA; IKARO ALVES DE ANDRADE, UNIVERSIDADE DE BRASÍLIA; JOÃO MARCOS FAGUNDES SILVA, UNIVERSIDADE DE BRASÍLIA; FERNANDO LUCAS DE MELO, UNIVERSIDADE DE BRASILIA; ANA MARIA MACHADO, COMPANHIA DE SANEAMENTO AMBIENTAL DO DF; ALICE KAZUKO INOUE NAGATA, CNPH; TATSUYA NAGATA, UNIVERSIDADE DE BRASÍLIA. |
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Título: |
Metagenomic analyses of plant virus sequences in sewage water for plant viruses monitoring. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Tropical Plant Pathology, v. 48, p. 408-416, 2023. |
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DOI: |
10.1007/s40858-023-00575-8 |
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Idioma: |
Inglês |
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Conteúdo: |
Frequent monitoring of emerging viruses of agricultural crops is one of the most important missions for plant virologists. A fast and precise identifcation of potential harmful viruses may prevent the occurrence of serious epidemics. Nowadays, highthroughput sequencing (HTS) technologies became an accessible and powerful tool for this purpose. The major discussion of this strategy resides in the process of sample collection, which is usually laborious, costly and nonrepresentative. In this study, we assessed the use of sewage water samples for monitoring the widespread, numerous, and stable plant viruses using HTS analysis and RT-qPCR. Plant viruses belonged to 12 virus families were found, from which Virgaviridae, Solemoviridae, Tymoviridae, Alphafexiviridae, Betafexiviridae, losteroviridae and Secoviridae were the most abundant ones with more than 20 species. Additionally, we detected one quarantine virus in Brazil and a new tobamovirus species. To assess the importance of the processed foods as virus release origins to sewage, we selected two viruses, the tobamovirus pepper mild mottle virus (PMMoV) and the carlavirus garlic common latent virus (GarCLV), to detect in processed food materials by RT-qPCR. PMMoV was detected in large amount in pepper-based processed foods and in sewage samples, while GarCLV was less frequent in dried and fresh garlic samples, and in the sewage samples. This suggested a high correlation of virus abundance in sewage and processed food sources. The potential use of sewage for a virus survey is discussed in this study. MenosFrequent monitoring of emerging viruses of agricultural crops is one of the most important missions for plant virologists. A fast and precise identifcation of potential harmful viruses may prevent the occurrence of serious epidemics. Nowadays, highthroughput sequencing (HTS) technologies became an accessible and powerful tool for this purpose. The major discussion of this strategy resides in the process of sample collection, which is usually laborious, costly and nonrepresentative. In this study, we assessed the use of sewage water samples for monitoring the widespread, numerous, and stable plant viruses using HTS analysis and RT-qPCR. Plant viruses belonged to 12 virus families were found, from which Virgaviridae, Solemoviridae, Tymoviridae, Alphafexiviridae, Betafexiviridae, losteroviridae and Secoviridae were the most abundant ones with more than 20 species. Additionally, we detected one quarantine virus in Brazil and a new tobamovirus species. To assess the importance of the processed foods as virus release origins to sewage, we selected two viruses, the tobamovirus pepper mild mottle virus (PMMoV) and the carlavirus garlic common latent virus (GarCLV), to detect in processed food materials by RT-qPCR. PMMoV was detected in large amount in pepper-based processed foods and in sewage samples, while GarCLV was less frequent in dried and fresh garlic samples, and in the sewage samples. This suggested a high correlation of virus abundance in sewage and processed food sources.... Mostrar Tudo |
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Palavras-Chave: |
High-throughput sequencing. |
|
Thesagro: |
Vírus. |
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Thesaurus NAL: |
Wastewater. |
|
Categoria do assunto: |
S Ciências Biológicas |
|
Marc: |
LEADER 02294naa a2200241 a 4500
001 2161643
005 2024-02-05
008 2023 bl uuuu u00u1 u #d
024 7 $a10.1007/s40858-023-00575-8$2DOI
100 1 $aDUARTE, M. F.
245 $aMetagenomic analyses of plant virus sequences in sewage water for plant viruses monitoring.$h[electronic resource]
260 $c2023
520 $aFrequent monitoring of emerging viruses of agricultural crops is one of the most important missions for plant virologists. A fast and precise identifcation of potential harmful viruses may prevent the occurrence of serious epidemics. Nowadays, highthroughput sequencing (HTS) technologies became an accessible and powerful tool for this purpose. The major discussion of this strategy resides in the process of sample collection, which is usually laborious, costly and nonrepresentative. In this study, we assessed the use of sewage water samples for monitoring the widespread, numerous, and stable plant viruses using HTS analysis and RT-qPCR. Plant viruses belonged to 12 virus families were found, from which Virgaviridae, Solemoviridae, Tymoviridae, Alphafexiviridae, Betafexiviridae, losteroviridae and Secoviridae were the most abundant ones with more than 20 species. Additionally, we detected one quarantine virus in Brazil and a new tobamovirus species. To assess the importance of the processed foods as virus release origins to sewage, we selected two viruses, the tobamovirus pepper mild mottle virus (PMMoV) and the carlavirus garlic common latent virus (GarCLV), to detect in processed food materials by RT-qPCR. PMMoV was detected in large amount in pepper-based processed foods and in sewage samples, while GarCLV was less frequent in dried and fresh garlic samples, and in the sewage samples. This suggested a high correlation of virus abundance in sewage and processed food sources. The potential use of sewage for a virus survey is discussed in this study.
650 $aWastewater
650 $aVírus
653 $aHigh-throughput sequencing
700 1 $aANDRADE, I. A. de
700 1 $aSILVA, J. M. F.
700 1 $aMELO, F. L. de
700 1 $aMACHADO, A. M.
700 1 $aINOUE-NAGATA, A. K.
700 1 $aNAGATA, T.
773 $tTropical Plant Pathology$gv. 48, p. 408-416, 2023.
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