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| 20. |  | MOURA, M. T.; PAULINI, F.; OLIVEIRA, G. S.; MELO, E. de O.; RUMPF, R. Expressão da proteína fluorescente verde (EGFP) em fibroblastos de bovinos adultos da raça Junqueira. In: CONGRESSO BRASILEIRO DE GENÉTICA, 55., 2009, Águas de Lindóia, SP. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2009. p. 213.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Tabuleiros Costeiros. |
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Data corrente: |
17/09/2019 |
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Data da última atualização: |
05/09/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
CHAVES, M. S.; LUZ, V. B.; FERREIRA-SILVA, J. C.; MELO, E. de O.; PAIVA, S. R.; BARROS, I. de; BARTOLOMEU, C. C.; AZEVEDO, H. C.; OLIVEIRA, M. A. L. |
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Afiliação: |
Maiana Silva Chaves, Universidade Federal de Pernambuco; Valesca Barreto Luz, Centro Universitário CESMAC, Maceió; José Carlos Ferreira-Silva, Universidade Federal de Pernambuco; EDUARDO DE OLIVEIRA MELO, Cenargen; Samuel Rezende Paiva, UFT; INACIO DE BARROS, CNPGL; Cláudio Coutinho Bartolomeu, Universidade Federal de Pernambuco; HYMERSON COSTA AZEVEDO, CPATC; Marcos Antonio Lemos Oliveira, Universidade Federal de Pernambuco. |
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Título: |
Ovarian and follicular variables used to determine ewes with different FecGE genotypes. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Animal Reproduction Science, v. 208, 2019, 106117 |
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Páginas: |
7 p. |
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DOI: |
https://doi.org/10.1016/j.anireprosci.2019.106117 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract
Based on ovarian and follicular variables, there was determination of ewes with different FecGE genotypes. Based on the FecGE genotype, 65 Santa Inês ewes were assigned to three experimental groups: homozygous wild-type (n = 25; FecG+/+), mutant heterozygous (n = 27; FecG+/E) and mutant homozygous (n = 13; FecGE/E). The ewe?s ovaries were weighed and measured, then the follicles (oocyte, nucleus and nucleolus) were histologically evaluated for morphometry and morphology. Morphologically normal follicles, in the primordial and transitional stages, explained 70.18% of the variability morphological characteristics between mutant and wild-type ewes. Conducting the morphometric evaluation resulted in a more precise determination of the genotype groups when there was assessment of the primordial and secondary follicular developmental stages. The diameter of the oocyte and the oocyte nucleus of the primordial follicles explained 36.76% of the variability in follicular morphology between ewes with the mutation and those with the wildtype group. Similarly, the core diameter of oocytes in secondary follicles explained 10.63% of the variability in follicular morphology among FecGE/E, FecG+/E and FecG+/+ ewes. Thus, morphologically normal follicles in the primordial and transitional stages of development are the variables that allow for a more precise differentiation of Santa Inês ewes with the FecGE mutation. These variables may be evaluated to make more efficient the adoption of biotechniques that when conducted there is utilisation of follicles in the initial developmental stages as a physiological basis for classifying whether specific follicles are useful when conducting the techniques. MenosAbstract
Based on ovarian and follicular variables, there was determination of ewes with different FecGE genotypes. Based on the FecGE genotype, 65 Santa Inês ewes were assigned to three experimental groups: homozygous wild-type (n = 25; FecG+/+), mutant heterozygous (n = 27; FecG+/E) and mutant homozygous (n = 13; FecGE/E). The ewe?s ovaries were weighed and measured, then the follicles (oocyte, nucleus and nucleolus) were histologically evaluated for morphometry and morphology. Morphologically normal follicles, in the primordial and transitional stages, explained 70.18% of the variability morphological characteristics between mutant and wild-type ewes. Conducting the morphometric evaluation resulted in a more precise determination of the genotype groups when there was assessment of the primordial and secondary follicular developmental stages. The diameter of the oocyte and the oocyte nucleus of the primordial follicles explained 36.76% of the variability in follicular morphology between ewes with the mutation and those with the wildtype group. Similarly, the core diameter of oocytes in secondary follicles explained 10.63% of the variability in follicular morphology among FecGE/E, FecG+/E and FecG+/+ ewes. Thus, morphologically normal follicles in the primordial and transitional stages of development are the variables that allow for a more precise differentiation of Santa Inês ewes with the FecGE mutation. These variables may be evaluated... Mostrar Tudo |
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Palavras-Chave: |
GDF-9; Ovine; Prolificacy. |
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Thesaurus NAL: |
Ovulation; Polymorphism. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02612naa a2200301 a 4500
001 2112265
005 2022-09-05
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.anireprosci.2019.106117$2DOI
100 1 $aCHAVES, M. S.
245 $aOvarian and follicular variables used to determine ewes with different FecGE genotypes.$h[electronic resource]
260 $c2019
300 $a7 p.
520 $aAbstract Based on ovarian and follicular variables, there was determination of ewes with different FecGE genotypes. Based on the FecGE genotype, 65 Santa Inês ewes were assigned to three experimental groups: homozygous wild-type (n = 25; FecG+/+), mutant heterozygous (n = 27; FecG+/E) and mutant homozygous (n = 13; FecGE/E). The ewe?s ovaries were weighed and measured, then the follicles (oocyte, nucleus and nucleolus) were histologically evaluated for morphometry and morphology. Morphologically normal follicles, in the primordial and transitional stages, explained 70.18% of the variability morphological characteristics between mutant and wild-type ewes. Conducting the morphometric evaluation resulted in a more precise determination of the genotype groups when there was assessment of the primordial and secondary follicular developmental stages. The diameter of the oocyte and the oocyte nucleus of the primordial follicles explained 36.76% of the variability in follicular morphology between ewes with the mutation and those with the wildtype group. Similarly, the core diameter of oocytes in secondary follicles explained 10.63% of the variability in follicular morphology among FecGE/E, FecG+/E and FecG+/+ ewes. Thus, morphologically normal follicles in the primordial and transitional stages of development are the variables that allow for a more precise differentiation of Santa Inês ewes with the FecGE mutation. These variables may be evaluated to make more efficient the adoption of biotechniques that when conducted there is utilisation of follicles in the initial developmental stages as a physiological basis for classifying whether specific follicles are useful when conducting the techniques.
650 $aOvulation
650 $aPolymorphism
653 $aGDF-9
653 $aOvine
653 $aProlificacy
700 1 $aLUZ, V. B.
700 1 $aFERREIRA-SILVA, J. C.
700 1 $aMELO, E. de O.
700 1 $aPAIVA, S. R.
700 1 $aBARROS, I. de
700 1 $aBARTOLOMEU, C. C.
700 1 $aAZEVEDO, H. C.
700 1 $aOLIVEIRA, M. A. L.
773 $tAnimal Reproduction Science$gv. 208, 2019, 106117
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