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| Registros recuperados : 24 | |
| 6. |  | INGLIS, P. W.; RIGDEN, D. J.; MELLO, L. V.; LOUIS, E. J.; VALADARES-INGLIS, M. C. Monomorphic subtelomeric DNA in the filamentous fungus, Metarhizium anisopliae, contains a RecQ helicase-like gene. Molecular Genetics and Genomics, v. 274, p. 79-90, 2005. Published online: 2 June 2005.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | RIBEIRO, S. G.; MELLO, L. V.; BOITEUX, L. S.; KITAJIMA, E. W.; FARIA, J. C. Tomato infection by a geminivirus in the Federal District, Brazil. Fitopatologia Brasileira, Brasilia, v.19, p.330, ago. 1994. Resumo. Suplemento.| Biblioteca(s): Embrapa Hortaliças. |
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| 9. | | DA SILVA, M. C. M.; NESHICH, G.; TOGAWA, R. C.; MELLO, L. V.; CHRISPEELS, M.; GROSSI-de-SÁ, M. F. Interações moleculares entre alpha-amilases e inibidores de plantas. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 5., 2000, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2000. p. 34.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | CASTRO, F. C.; VALLS, F. S.; BATISTA, J. A. N.; GROSSI-de-SÁ, M. F.; MELLO, L. V. Amplificação por RT-PCR e clonagem de um gene e alfa-amilase de Rhyzopertha dominica (L.). In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 7., 2002, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2002. p. 29.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | FRANCO, O. L.; SA, M. F. G. de; SALES, M. P.; MELLO, L. V.; OLIVEIRA, A. S.; RIGDEN, D. J. Overlapping binding sites for trypsin and papain on a kunitz-type proteinase inhibitor from Prosopis juliflora. Protein: Structure, Function, and Genetics, v. 49, p. 335-341, 2002.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 13. | | FERREIRA, P.; PAES, N. S.; SOUZA, C. R. B.; GERHARDT, I. R.; COUTINHO, M. V.; MELLO, L. V.; SOUSA, M. V.; GROSSI DE SA, M. F. Arcelin 5 protein: purification, analysis, developing seeds study and imunocytolocalization. In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUIMICA E BIOLOGIA MOLECULAR, 23., 1994, Caxambu, MG. Programa e resumos. Caxambu: Sociedade Brasileira de Bioquimica e Biologia Molecular, 1994. p.49. E-39. p.49 Resumo.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 14. | | CARVALHO, P. G. B. de; BLOCH JUNIOR, C.; MORHY, L.; SILVA, M. C. M. da; MELLO, L. V. de; NESHICH, G. The amino acid sequence of the Phaseolus vulgaris var. "Fogo na Serra" inhibitor and interactive surface modeling for the enzyme inhibitor complex. Journal of Protein Chemistry, New York, v.15, n.6, p.591-598, 1996.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 15. | | PAES, N. S.; FERREIRA, P.; SOUZA, C. R. B.; GERHARDT, I. R.; VENTURA, M.; MELLO, L. V.; SOUSA, M. V.; SA, M. F. G. de. Purification and analysis of arcelin 5 protein from wild bean. In: ENCONTRO BRASILEIRO DE BIOTECNOLOGIA VEGETAL, l.,1993, Brasilia. Programas e resumos. Brasilia: EMBRAPA-CENARGEN, 1993. Poster 032. Resumo| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 16. | | SILVA, M. C. M. da; MELLO, L. V.; COUTINHO, M. V.; RIGDEN, D. J.; NESHICH, G.; CHRISPEELS, M. J.; GROSSI-DE-SÁ, M. F. Mutants of common bean alpha-amylase inhibitor-2 as an approach to investigate binding specificity to alpha-amylases. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 39, n. 3, p. 201-208, mar. 2004.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Recursos Genéticos e Biotecnologia. |
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| 17. | | SILVA, M. C. M. da; MELLO, L. V.; COUTINHO, M. V.; RIGDEN, D. J.; NESHICH, G.; CHRISPEELS, M. J.; SA, M. F. G. de. Mutants of common bean alpha-amylase inhibitor-2 as an approach to investigate binding specificity to alpha-amylases. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 39, n. 3, p. 201-208, mar. 2004 Titulo em português: Mutantes do inibidor-2 de alfa-amilase do feijão-comum para investigação da especificidade de ligação a alfa-amilases.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 18. | | FRASER, J.; MELLO, L. V. de; WARD, D.; REES, H. H.; WILLIAMS, D. R.; FANG, Y.; BRASS, A.; GRACEY, A. Y.; COSSINS, A. R. Hypoxia-inducible myoglobin expression in nonmuscle tissues. PNAS, v. 103, n. 8, p. 2977-2981, 2006.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 19. | | PELEGRINI, P. B.; MURAD, A. M.; GROSSI-DE-SÁ, M. F.; MELLO, L. V.; ROMEIRO, L. A. S.; NORONHA, E. F.; CALDAS, R. A; FRANCO, O. L. Structure and enzyme properties of Zabrotes subfasciatus alfa-amylase. Archives of Insect Biochemistry and Physiology, v. 61, p. 77-86, 2006.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 20. | | DUSI, D. M. de A.; ALVES, E. R.; CABRAL, G. B.; MELLO, L. V.; RIGDEN, D. J.; SILVEIRA, E. D.; ALVES‑FERREIRA, M.; GUIMARÃES, L. A.; GOMES, A. C. M. M.; RODRIGUES, J. C. M.; CARNEIRO, V. T. de C. An exonuclease V homologue is expressed predominantly during early megasporogenesis in apomictic Brachiaria brizantha. Planta, v. 258, 2023. Article number: 5. Na publicação: Diva M. A. Dusi, Vera T. C. Carneiro.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 24 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
27/07/2005 |
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Data da última atualização: |
11/05/2022 |
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Autoria: |
INGLIS, P. W.; RIGDEN, D. J.; MELLO, L. V.; LOUIS, E. J.; VALADARES-INGLIS, M. C. |
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Título: |
Monomorphic subtelomeric DNA in the filamentous fungus, Metarhizium anisopliae, contains a RecQ helicase-like gene. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Molecular Genetics and Genomics, v. 274, p. 79-90, 2005. |
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Idioma: |
Inglês |
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Notas: |
Published online: 2 June 2005. |
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Conteúdo: |
In most filamentous fungi, telomere-associated sequences (TASs) are polymorphic, and the presence of restriction fragment length polymorphisms (RFLPs) may permit the number of chromosome ends to be estimated from the number of telomeric bands obtained by restriction digestion. Here, we describe strains of Metarhizium, Gliocladium and Paecilomyces species in which only one or a few telomeric bands of unequal intensity are detectable by Southern hybridization, indicating that interchromosomal TAS exchange occurs. We also studied an anomalous strain of Metarhizium anisopliae, which produces polymorphic telomeric bands larger than 8 kb upon digestion of genomic DNA with XhoI. In this case, the first XhoI site in from the chromosome end must lie beyond the presumed monomorphic region. Cloned telomeres from this strain comprise 18?26 TTAGGG repeats, followed at the internal end of the telomere tract by five repeats of the telomere-like sequence TAAACGCTGG. An 8.1-kb TAS clone also contains a gene for a RecQ-like helicase, designated TAH1, suggesting that this TAS is analogous to the Y elements in yeast and the subtelomeric helicase ORFs of Ustilago maydis (UTASRecQ) and Magnaporthe grisea (TLH1). The TAS in the anomalous strain of M. anisopliae, however, appears distinct from these in that it is found at most telomeres and its predicted protein product possesses a significantly longer N-terminal region in comparison to the M. grisea and U. maydis helicases. Hybridization analyses showed that TAH1 homologues are present in all other anomalous M. anisopliae strains studied, as well as in some other polymorphic strains, where the recQ-like gene also appears to be telomere-associated. MenosIn most filamentous fungi, telomere-associated sequences (TASs) are polymorphic, and the presence of restriction fragment length polymorphisms (RFLPs) may permit the number of chromosome ends to be estimated from the number of telomeric bands obtained by restriction digestion. Here, we describe strains of Metarhizium, Gliocladium and Paecilomyces species in which only one or a few telomeric bands of unequal intensity are detectable by Southern hybridization, indicating that interchromosomal TAS exchange occurs. We also studied an anomalous strain of Metarhizium anisopliae, which produces polymorphic telomeric bands larger than 8 kb upon digestion of genomic DNA with XhoI. In this case, the first XhoI site in from the chromosome end must lie beyond the presumed monomorphic region. Cloned telomeres from this strain comprise 18?26 TTAGGG repeats, followed at the internal end of the telomere tract by five repeats of the telomere-like sequence TAAACGCTGG. An 8.1-kb TAS clone also contains a gene for a RecQ-like helicase, designated TAH1, suggesting that this TAS is analogous to the Y elements in yeast and the subtelomeric helicase ORFs of Ustilago maydis (UTASRecQ) and Magnaporthe grisea (TLH1). The TAS in the anomalous strain of M. anisopliae, however, appears distinct from these in that it is found at most telomeres and its predicted protein product possesses a significantly longer N-terminal region in comparison to the M. grisea and U. maydis helicases. Hybridization analyses ... Mostrar Tudo |
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Palavras-Chave: |
Recombination; RecQ helicase; Telomere associated sequence. |
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Thesagro: |
Fungo; Metarhizium Anisopliae. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178387/1/ID-253071.pdf
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Marc: |
LEADER 02462naa a2200241 a 4500
001 1185898
005 2022-05-11
008 2005 bl uuuu u00u1 u #d
100 1 $aINGLIS, P. W.
245 $aMonomorphic subtelomeric DNA in the filamentous fungus, Metarhizium anisopliae, contains a RecQ helicase-like gene.$h[electronic resource]
260 $c2005
500 $aPublished online: 2 June 2005.
520 $aIn most filamentous fungi, telomere-associated sequences (TASs) are polymorphic, and the presence of restriction fragment length polymorphisms (RFLPs) may permit the number of chromosome ends to be estimated from the number of telomeric bands obtained by restriction digestion. Here, we describe strains of Metarhizium, Gliocladium and Paecilomyces species in which only one or a few telomeric bands of unequal intensity are detectable by Southern hybridization, indicating that interchromosomal TAS exchange occurs. We also studied an anomalous strain of Metarhizium anisopliae, which produces polymorphic telomeric bands larger than 8 kb upon digestion of genomic DNA with XhoI. In this case, the first XhoI site in from the chromosome end must lie beyond the presumed monomorphic region. Cloned telomeres from this strain comprise 18?26 TTAGGG repeats, followed at the internal end of the telomere tract by five repeats of the telomere-like sequence TAAACGCTGG. An 8.1-kb TAS clone also contains a gene for a RecQ-like helicase, designated TAH1, suggesting that this TAS is analogous to the Y elements in yeast and the subtelomeric helicase ORFs of Ustilago maydis (UTASRecQ) and Magnaporthe grisea (TLH1). The TAS in the anomalous strain of M. anisopliae, however, appears distinct from these in that it is found at most telomeres and its predicted protein product possesses a significantly longer N-terminal region in comparison to the M. grisea and U. maydis helicases. Hybridization analyses showed that TAH1 homologues are present in all other anomalous M. anisopliae strains studied, as well as in some other polymorphic strains, where the recQ-like gene also appears to be telomere-associated.
650 $aFungo
650 $aMetarhizium Anisopliae
653 $aRecombination
653 $aRecQ helicase
653 $aTelomere associated sequence
700 1 $aRIGDEN, D. J.
700 1 $aMELLO, L. V.
700 1 $aLOUIS, E. J.
700 1 $aVALADARES-INGLIS, M. C.
773 $tMolecular Genetics and Genomics$gv. 274, p. 79-90, 2005.
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