Data/hora: 25/06/2024 17:57:45

Biblioteca(s): | Embrapa Meio Ambiente.
Data corrente: | 21/11/2019
Data da última atualização: | 21/11/2019
Autoria: | BAUERMEISTER, A.; CALIL, F. A.; PINTO, F. das C. L.; MEDEIROS, T. C. de T.; ALMEIDA, L. C. de; SILVA, L. J. da; MELO, I. S. de; ZUCCHI, T. D.; COSTA-LOTUFO, L. V.; MORAES, L. A. B.
Afiliação: | ANELIZE BAUERMEISTER, FFCLRP-USP; FELIPE ANTUNES CALIL, FFCLRP-USP; FRANCISCO DAS CHAGAS LIMA PINTO, UFC; TALITA CARLA DE TRALIA MEDEIROS, FFCLRP-USP; LARISSA COSTA DE ALMEIDA, ICB-USP; LEONARDO JOSE DA SILVA, ESALQ-USP; ITAMAR SOARES DE MELO, CNPMA; TIAGO DOMINGUES ZUCCHI, Agrivalle; LETICIA VERAS COSTA-LOTUFO, ICB-USP; LUIZ ALBERTO BERALDO DE MORAES, FFCLRP-USP.
Título: | Pradimicin-IRD from Amycolatopsis sp. IRD-009 and its antimicrobial and cytotoxic activities.
Ano de publicação: | 2019
Fonte/Imprenta: | Natural Product Research, v. 33, n. 12, p. 1713-1720, 2019.
ISSN: | 1478-6419
DOI: | https://doi.org/10.1080/14786419.2018.1434639
Idioma: | Inglês
Conteúdo: | A new polycyclic antibiotic, pradimicin-IRD, was isolated from actinobacteria Amycolatopsis sp. IRD-009 recovered from soil of Brazilian rainforest undergoing restoration area. This molecule is the major compound produced in solid culture media. The new compound was detected by a focused method of precursor ion (high-performance liquid chromatography coupled to tandem mass spectrometer) developed previously to identify unusual aminoglycosyl sugar moieties. The compound was isolated and its structure was, therefore, elucidated by high-resolution mass spectrometry, and 1D and 2D nuclear magnetic resonance experiments. Pradimicin- IRD displayed potential antimicrobial activity against Streptococcus agalactiae (MIC 3.1 &#956;g/mL), Pseudomonas aeruginosa (MIC 3.1 &#956;g/ mL) and Staphylococcus aureus (MIC 3.1 &#956;g/mL), and also cytotoxicity against tumour and non-tumour cell lines with IC 50 values ranging from 0.8 &#956;M in HCT-116 colon carcinoma cells to 2.7 &#956;M in MM 200 melanoma cells. Particularly, these biological properties are described for the first time for this chemical class.
Palavras-Chave: | Antimicrobial properties.
Thesagro: | Antibiótico; Bactéria.
Thesaurus Nal: | Actinobacteria; Amycolatopsis; Antibiotics.
Categoria do assunto: | S Ciências Biológicas
Registro original: | Embrapa Meio Ambiente (CNPMA)
Exemplares: | CNPMA - (16506-1) - UPC - AP - DD -  -  -  - DIS