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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
17/11/2014 |
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Data da última atualização: |
17/11/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VALENTE, A. M. M. P.; BOFFO, E. F.; MELO, I. S. de; FERREIRA, A. G. |
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Afiliação: |
ANGELA MARIA MONTES PERAL VALENTE, UFSCar; ELISANGELA FABIANA BOFFO, UFBA; ITAMAR SOARES DE MELO, CNPMA; ANTONIO G FERREIRA, IFSCar. |
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Título: |
Quantification of mycophenolic acid and citrinin produced by Penicillium sp. using 1H NMR. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Applied Magnetic Resonance, Wien, v. 45, n. 3, p. 207-215, 2014. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This study proposes a simple methodology to construct the production curves of mycophenolic acid by Penicillium sp. (CASP5) and citrinin by Penicillium sp. (CATL1.1) in the crude extract, without any purification. The quantification of the compounds was done by 1H nuclear magnetic resonance (NMR) using the signal integration and an internal standard, N,N-dimethylformamide. Fungi were cultivated for a period of 20 days and quantification of the metabolites in the extracts was done starting from time zero, 2 days and after this period in an interval of 4 days. The maximum production of mycophenolic acid and citrinin was obtained at 12 and 8 days of fermentation, respectively. These results show that the 1H NMR technique was efficient to define the production curves of mycophenolic acid and citrinin directly in the crude extracts. In addition, the technique made it possible to evaluate the purity degree of the substances obtained in the extraction process. Furthermore, this is the first study that uses the 1H NMR technique to determine the production curves of secondary metabolites. |
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Palavras-Chave: |
Mycophenolic acid; NMR; Penicillium sp. |
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Thesagro: |
Análise de laboratório; Fungo. |
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Thesaurus Nal: |
Citrinin; Nuclear magnetic resonance spectroscopy. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/111791/1/2014AP014.pdf
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Marc: |
LEADER 01835naa a2200241 a 4500
001 2000338
005 2014-11-17
008 2014 bl uuuu u00u1 u #d
100 1 $aVALENTE, A. M. M. P.
245 $aQuantification of mycophenolic acid and citrinin produced by Penicillium sp. using 1H NMR.$h[electronic resource]
260 $c2014
520 $aAbstract: This study proposes a simple methodology to construct the production curves of mycophenolic acid by Penicillium sp. (CASP5) and citrinin by Penicillium sp. (CATL1.1) in the crude extract, without any purification. The quantification of the compounds was done by 1H nuclear magnetic resonance (NMR) using the signal integration and an internal standard, N,N-dimethylformamide. Fungi were cultivated for a period of 20 days and quantification of the metabolites in the extracts was done starting from time zero, 2 days and after this period in an interval of 4 days. The maximum production of mycophenolic acid and citrinin was obtained at 12 and 8 days of fermentation, respectively. These results show that the 1H NMR technique was efficient to define the production curves of mycophenolic acid and citrinin directly in the crude extracts. In addition, the technique made it possible to evaluate the purity degree of the substances obtained in the extraction process. Furthermore, this is the first study that uses the 1H NMR technique to determine the production curves of secondary metabolites.
650 $aCitrinin
650 $aNuclear magnetic resonance spectroscopy
650 $aAnálise de laboratório
650 $aFungo
653 $aMycophenolic acid
653 $aNMR
653 $aPenicillium sp
700 1 $aBOFFO, E. F.
700 1 $aMELO, I. S. de
700 1 $aFERREIRA, A. G.
773 $tApplied Magnetic Resonance, Wien$gv. 45, n. 3, p. 207-215, 2014.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
26/12/2013 |
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Data da última atualização: |
03/01/2014 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
VILARINHO, A. A.; PEDROZO, C. A.; MEDEIROS, R. D. de; ROCHA, L. M. P. da. |
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Afiliação: |
ALOISIO ALCANTARA VILARINHO, CPAF-RR; CASSIA ANGELA PEDROZO, CPAF-RR; ROBERTO DANTAS DE MEDEIROS, CPAF-RR; LEONARDO MELO PEREIRA DA ROCHA, CNPMS. |
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Título: |
Desempenho agronômico de genótipos de milho no estado de Roraima. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 7., 2013, Uberlândia. Variedade melhorada: a força da nossa agricultura: anais. Viçosa, MG: SBMP, 2013. |
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Páginas: |
p. 2630-2633. |
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Idioma: |
Português |
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Thesagro: |
Genótipo; Melhoramento genético vegetal. |
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Thesaurus NAL: |
Genotype; Plant breeding. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/94579/1/Desempenho-agronomico-2.pdf
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Marc: |
LEADER 00697nam a2200193 a 4500
001 1974608
005 2014-01-03
008 2013 bl uuuu u00u1 u #d
100 1 $aVILARINHO, A. A.
245 $aDesempenho agronômico de genótipos de milho no estado de Roraima.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 7., 2013, Uberlândia. Variedade melhorada: a força da nossa agricultura: anais. Viçosa, MG: SBMP$c2013
300 $ap. 2630-2633.
650 $aGenotype
650 $aPlant breeding
650 $aGenótipo
650 $aMelhoramento genético vegetal
700 1 $aPEDROZO, C. A.
700 1 $aMEDEIROS, R. D. de
700 1 $aROCHA, L. M. P. da
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Embrapa Milho e Sorgo (CNPMS) |
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