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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria de Alimentos. Para informações adicionais entre em contato com ctaa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
27/11/2020 |
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Data da última atualização: |
09/05/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ROLIM, F. R. L.; OLIVEIRA, C. J. B.; FREITAS NETO, O. C. de; SANTOS, K. M. O. dos; GUERRA, G. C. B.; RODRIGUES, R. V.; ASSIS, P. O. A. de; ARAUJO, D. F. de S.; CARVALHO, V. A. G. de; LEMOS, M. L. P.; SILVA, N. M. V. da; SOARES, J. K.; GARCIA, H. E. M.; SOUZA, E. L. de; SOUZA, F. de A. L.; BARROS, M. E. G.; OLIVEIRA, M. E. G.; QUEIROGA, R. C. R. E. |
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Afiliação: |
Rolim, F. R. L., UFP; C. J. B. Oliveira, UFP; Freitas Neto, O.C. de, UFMG; KARINA MARIA OLBRICH DOS SANTOS, CTAA; G. C. B. Guerra, UFRN; R. V. Rodrigues, UFCG; P.O.A. de Assis, UFPB; D. F. de S. Araújo, UFRJ; V. A. G. de Carvalho, UFPB; M. L. P. Lemos, UFPB; N. M. V. da Silva, UFPB; J. K. Soares, UFCG; H. E. M. GARCIA, UFPB; E. L. de SOUZA, UFPB; F. de A. L. SOUZA, UFRPE; M. E. G. de Barros, UFRPE; M. E. G. de Oliveira, UFPB; R. C. R. E. Queiroga, UFPB. |
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Título: |
Microbiological, immunological, and histological changes in the gut of Salmonella Enteritidis-challenged rats fed goat cheese containing Lactobacillus rhamnosus EM1107. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Journal of Dairy Science, v. 104, n. 1, p. 179-197, 2021. |
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DOI: |
https://doi.org/10.3168/jds.2020-18820. |
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Idioma: |
Inglês |
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Conteúdo: |
Cheeses are able to serve as suitable matrices for supplying probiotics to consumers, enabling appropriate conditions for bacteria to survive gastric transit and reach the gut, where they are assumed to promote beneficial processes. The present study aimed to evaluate the microbiological, immunological, and histological changes in the gut of Salmonella Enteritidis-challenged rats fed goat cheese supplemented with the probiotic strain Lactobacillus rhamnosus EM1107. Thirty male albino Wistar rats were randomly distributed into 5 experimental groups with 6 animals each: negative (NC) and positive (PtC) control groups, control goat cheese (CCh), goat cheese added with L. rhamnosus EM1107 (LrCh), and L. rhamnosus EM1107 only (EM1107). All animals, except NC group were challenged with Salmonella Enteritidis (109 colony-forming units in 1 mL of saline through oral gavage). Microbial composition was assessed with high-throughput 16S rRNA sequencing by means of Illumina MiSeq (Illumina, San Diego, CA). Nuclear factor kappa B (NF-?B) from the animal cecum tissue was determined by real-time PCR and interleukins (TNF-?, IL-1?, IL-10, and IFN-?) by means of ELISA. Myeloperoxidase and malondialdehyde levels were determined biochemically. The administration of the L. rhamnosus EM1107 probiotic strain, either as a pure culture or added to a cheese matrix, was able to reduce Salmonella colonization in the intestinal lumen and lessen tissue damage compared with rats from PtC group. In addition, the use of cheese for the probiotic strain delivery (LrCh) was associated with a marked shift in the gut microbiota composition toward the increase of beneficial organisms such as Blautia and Lactobacillus and a reduction in NF-?B expression. These findings support our hypothesis that cheeses might be explored as functional matrices for the efficacious delivery of probiotic strains to consumers. MenosCheeses are able to serve as suitable matrices for supplying probiotics to consumers, enabling appropriate conditions for bacteria to survive gastric transit and reach the gut, where they are assumed to promote beneficial processes. The present study aimed to evaluate the microbiological, immunological, and histological changes in the gut of Salmonella Enteritidis-challenged rats fed goat cheese supplemented with the probiotic strain Lactobacillus rhamnosus EM1107. Thirty male albino Wistar rats were randomly distributed into 5 experimental groups with 6 animals each: negative (NC) and positive (PtC) control groups, control goat cheese (CCh), goat cheese added with L. rhamnosus EM1107 (LrCh), and L. rhamnosus EM1107 only (EM1107). All animals, except NC group were challenged with Salmonella Enteritidis (109 colony-forming units in 1 mL of saline through oral gavage). Microbial composition was assessed with high-throughput 16S rRNA sequencing by means of Illumina MiSeq (Illumina, San Diego, CA). Nuclear factor kappa B (NF-?B) from the animal cecum tissue was determined by real-time PCR and interleukins (TNF-?, IL-1?, IL-10, and IFN-?) by means of ELISA. Myeloperoxidase and malondialdehyde levels were determined biochemically. The administration of the L. rhamnosus EM1107 probiotic strain, either as a pure culture or added to a cheese matrix, was able to reduce Salmonella colonization in the intestinal lumen and lessen tissue damage compared with rats from PtC group. In additi... Mostrar Tudo |
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Palavras-Chave: |
Gut microbiome. |
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Thesagro: |
Tecnologia de Alimento. |
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Thesaurus Nal: |
Goat cheese; Lactobacillus rhamnosus; Probiotics; Salmonella Enteritidis. |
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Categoria do assunto: |
Q Alimentos e Nutrição Humana |
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Marc: |
LEADER 03229naa a2200409 a 4500
001 2127153
005 2024-05-09
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3168/jds.2020-18820.$2DOI
100 1 $aROLIM, F. R. L.
245 $aMicrobiological, immunological, and histological changes in the gut of Salmonella Enteritidis-challenged rats fed goat cheese containing Lactobacillus rhamnosus EM1107.$h[electronic resource]
260 $c2021
520 $aCheeses are able to serve as suitable matrices for supplying probiotics to consumers, enabling appropriate conditions for bacteria to survive gastric transit and reach the gut, where they are assumed to promote beneficial processes. The present study aimed to evaluate the microbiological, immunological, and histological changes in the gut of Salmonella Enteritidis-challenged rats fed goat cheese supplemented with the probiotic strain Lactobacillus rhamnosus EM1107. Thirty male albino Wistar rats were randomly distributed into 5 experimental groups with 6 animals each: negative (NC) and positive (PtC) control groups, control goat cheese (CCh), goat cheese added with L. rhamnosus EM1107 (LrCh), and L. rhamnosus EM1107 only (EM1107). All animals, except NC group were challenged with Salmonella Enteritidis (109 colony-forming units in 1 mL of saline through oral gavage). Microbial composition was assessed with high-throughput 16S rRNA sequencing by means of Illumina MiSeq (Illumina, San Diego, CA). Nuclear factor kappa B (NF-?B) from the animal cecum tissue was determined by real-time PCR and interleukins (TNF-?, IL-1?, IL-10, and IFN-?) by means of ELISA. Myeloperoxidase and malondialdehyde levels were determined biochemically. The administration of the L. rhamnosus EM1107 probiotic strain, either as a pure culture or added to a cheese matrix, was able to reduce Salmonella colonization in the intestinal lumen and lessen tissue damage compared with rats from PtC group. In addition, the use of cheese for the probiotic strain delivery (LrCh) was associated with a marked shift in the gut microbiota composition toward the increase of beneficial organisms such as Blautia and Lactobacillus and a reduction in NF-?B expression. These findings support our hypothesis that cheeses might be explored as functional matrices for the efficacious delivery of probiotic strains to consumers.
650 $aGoat cheese
650 $aLactobacillus rhamnosus
650 $aProbiotics
650 $aSalmonella Enteritidis
650 $aTecnologia de Alimento
653 $aGut microbiome
700 1 $aOLIVEIRA, C. J. B.
700 1 $aFREITAS NETO, O. C. de
700 1 $aSANTOS, K. M. O. dos
700 1 $aGUERRA, G. C. B.
700 1 $aRODRIGUES, R. V.
700 1 $aASSIS, P. O. A. de
700 1 $aARAUJO, D. F. de S.
700 1 $aCARVALHO, V. A. G. de
700 1 $aLEMOS, M. L. P.
700 1 $aSILVA, N. M. V. da
700 1 $aSOARES, J. K.
700 1 $aGARCIA, H. E. M.
700 1 $aSOUZA, E. L. de
700 1 $aSOUZA, F. de A. L.
700 1 $aBARROS, M. E. G.
700 1 $aOLIVEIRA, M. E. G.
700 1 $aQUEIROGA, R. C. R. E.
773 $tJournal of Dairy Science$gv. 104, n. 1, p. 179-197, 2021.
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Registro original: |
Embrapa Agroindústria de Alimentos (CTAA) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
08/01/2021 |
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Data da última atualização: |
08/01/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
PAIXAO, P. E. G.; COUTO, M. V. S. do; SOUSA, N. da C.; ABEB, H. A.; REIS, R. G. A.; DIAS, J. A. R.; MENESES, J. O.; CUNHA, F. S.; SANTOS, T. B. R.; SILVA, I. C. A. da; MEDEIROS, E. dos S.; FUJIMOTO, R. Y. |
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Afiliação: |
PETERSON EMMANUEL GUIMARAES PAIXAO; MARCIA VALERIA SILVA DO COUTO; NATALINO DA COSTA SOUSA; HIGO ANDRADE ABEB; RYULLER GAMA ABREU REIS; JOEL ARTUR RODRIGUES DIAS; Juliana Oliveira Menesesa; FERNANDA SANTOS CUNHA; THAYS BRITO REIS SANTOS; IZADORA CIBELY ALVES DA SILVA; ESTELA DOS SANTOS MEDEIROS; RODRIGO YUDI FUJIMOTO, CPATC. |
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Título: |
Autochthonous bacterium Lactobacillus plantarum as probiotic supplementation for productive performance and sanitary improvements on clownfish Amphiprion ocellaris. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Aquaculture, v. 526, 735395, 2020. |
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DOI: |
doi.org/10.1016/j.aquaculture.2020.735395 |
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Idioma: |
Inglês |
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Conteúdo: |
This current study evaluated the probiotic dietary supplementation with autochthonous bacterium Lactobacillus plantarum for Amphiprion ocellaris. Therefore, it was performed an experiment in complete randomized design with four treatments (T1: Control without probiotic; T2: 104 ; T3: 106 and T4: 108 CFU/g of ration) and three replicates. Juvenile fish (n = 120; 0.46 ± 0.01 g and 0.26 ± 0.06 cm) were distributed in twelve aquarium(80 L) and it received trial meal three times a day at fed rate of 4% live weight during 90 days. The fish were monthly measured and determined the total length, standard length, height, weight gain, biomass gain, specific growth rate, apparently feed conversion, relative condition factor, uniformity and survival. At the end of experiment (90 days) fish were submitted to hematological (n = 9/ treatment; Erythrogram, Leukogram and Thrombogram) and histological analysis (n = 6/ treatment; Intestinal tract). Also, after the supplementation period, the fish were challenged with pathogenic bacteria and the survival and blood analyzed. Results showed the fish fed T3 and T4 had improvement on weight gain, feed conversion, specific growth rate, height and total height from the intestinal villi. Increases of red blood cell (Erythrocyte, hematocrit and hemoglobin) and white blood cell (Lymphocyte and monocyte) also observed to both treatments (T3 and T4), However the T4 promoted the greater fish resistance to pathogenic bacteria. As conclusion, probiotic supplementation with the autochthonous bacterium Lactobacillus plantarum 106 CFU/g provides zootechnical improvement for clownfish rearing, but to improve the pathogenic infection resistance, the 108 CFU/g presented more adequate. MenosThis current study evaluated the probiotic dietary supplementation with autochthonous bacterium Lactobacillus plantarum for Amphiprion ocellaris. Therefore, it was performed an experiment in complete randomized design with four treatments (T1: Control without probiotic; T2: 104 ; T3: 106 and T4: 108 CFU/g of ration) and three replicates. Juvenile fish (n = 120; 0.46 ± 0.01 g and 0.26 ± 0.06 cm) were distributed in twelve aquarium(80 L) and it received trial meal three times a day at fed rate of 4% live weight during 90 days. The fish were monthly measured and determined the total length, standard length, height, weight gain, biomass gain, specific growth rate, apparently feed conversion, relative condition factor, uniformity and survival. At the end of experiment (90 days) fish were submitted to hematological (n = 9/ treatment; Erythrogram, Leukogram and Thrombogram) and histological analysis (n = 6/ treatment; Intestinal tract). Also, after the supplementation period, the fish were challenged with pathogenic bacteria and the survival and blood analyzed. Results showed the fish fed T3 and T4 had improvement on weight gain, feed conversion, specific growth rate, height and total height from the intestinal villi. Increases of red blood cell (Erythrocyte, hematocrit and hemoglobin) and white blood cell (Lymphocyte and monocyte) also observed to both treatments (T3 and T4), However the T4 promoted the greater fish resistance to pathogenic bacteria. As conclusion, probiotic suppl... Mostrar Tudo |
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Thesagro: |
Peixe; Peixe Ornamental. |
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Thesaurus NAL: |
Ornamental fish. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/220082/1/palhaco-2020.pdf
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Marc: |
LEADER 02677naa a2200301 a 4500
001 2129149
005 2021-01-08
008 2020 bl uuuu u00u1 u #d
024 7 $adoi.org/10.1016/j.aquaculture.2020.735395$2DOI
100 1 $aPAIXAO, P. E. G.
245 $aAutochthonous bacterium Lactobacillus plantarum as probiotic supplementation for productive performance and sanitary improvements on clownfish Amphiprion ocellaris.$h[electronic resource]
260 $c2020
520 $aThis current study evaluated the probiotic dietary supplementation with autochthonous bacterium Lactobacillus plantarum for Amphiprion ocellaris. Therefore, it was performed an experiment in complete randomized design with four treatments (T1: Control without probiotic; T2: 104 ; T3: 106 and T4: 108 CFU/g of ration) and three replicates. Juvenile fish (n = 120; 0.46 ± 0.01 g and 0.26 ± 0.06 cm) were distributed in twelve aquarium(80 L) and it received trial meal three times a day at fed rate of 4% live weight during 90 days. The fish were monthly measured and determined the total length, standard length, height, weight gain, biomass gain, specific growth rate, apparently feed conversion, relative condition factor, uniformity and survival. At the end of experiment (90 days) fish were submitted to hematological (n = 9/ treatment; Erythrogram, Leukogram and Thrombogram) and histological analysis (n = 6/ treatment; Intestinal tract). Also, after the supplementation period, the fish were challenged with pathogenic bacteria and the survival and blood analyzed. Results showed the fish fed T3 and T4 had improvement on weight gain, feed conversion, specific growth rate, height and total height from the intestinal villi. Increases of red blood cell (Erythrocyte, hematocrit and hemoglobin) and white blood cell (Lymphocyte and monocyte) also observed to both treatments (T3 and T4), However the T4 promoted the greater fish resistance to pathogenic bacteria. As conclusion, probiotic supplementation with the autochthonous bacterium Lactobacillus plantarum 106 CFU/g provides zootechnical improvement for clownfish rearing, but to improve the pathogenic infection resistance, the 108 CFU/g presented more adequate.
650 $aOrnamental fish
650 $aPeixe
650 $aPeixe Ornamental
700 1 $aCOUTO, M. V. S. do
700 1 $aSOUSA, N. da C.
700 1 $aABEB, H. A.
700 1 $aREIS, R. G. A.
700 1 $aDIAS, J. A. R.
700 1 $aMENESES, J. O.
700 1 $aCUNHA, F. S.
700 1 $aSANTOS, T. B. R.
700 1 $aSILVA, I. C. A. da
700 1 $aMEDEIROS, E. dos S.
700 1 $aFUJIMOTO, R. Y.
773 $tAquaculture$gv. 526, 735395, 2020.
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Embrapa Tabuleiros Costeiros (CPATC) |
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