Data/hora: 22/06/2024 20:54:53

Biblioteca(s): 	Embrapa Gado de Leite.	
Data corrente: 	08/02/2012	
Data da última atualização: 	05/02/2024	
Autoria: 	SANTOS, M. D. R.; VITOR, A. DE P.; CARNEIRO, J. da C.; PACIULLO, D. S. C.; MATOS, R. C.; MATOS, M. A. C.	
Afiliação: 	MELLINA DAMASCENO RACHID SANTOS, UFJF; ALINE DE PAULA VITOR, UFJF; JAILTON DA COSTA CARNEIRO, CNPGL; DOMINGOS SAVIO CAMPOS PACIULLO, CNPGL; RENATO CAMARGO MATOS, UFJF; MARIA AUXILIADORA COSTA MATOS, UFJF.	
Título: 	Use of ultrasound bath in the extraction and quantification of Ester-Linked Phenolic Acids in tropical forages.	
Ano de publicação: 	2011	
Fonte/Imprenta: 	American Journal of Analytical Chemistry, v. 2, p. 344-351, 2011.	
DOI: 	http://dx.doi.org/10.4236/ajac.2011.23042	
Idioma: 	Inglês	
Conteúdo: 	A method was developed for the analysis of ester-linked phenolic acids in forage samples using extraction by an ultrasound-assisted treatment and quantification by HPLC with a UV-VIS detector. A reversed-phase C18 column was used for developing the method and the optimal condition was established with isocratic elution using acetonitrile/methanol/H3PO4 pH 2.08 (13:12.5:74.5) as the mobile phase. To reduce the time of sample processing, the extraction of ester-linked phenolic acids was studied using ultrasound bath and the results were then compared with those from an extraction usual using alkaline hydrolysis (20°C for 24 h). The method was valued through external and internal calibration. Internal calibration using o-coumaric acid as internal standard and m-coumaric acid as surrogate internal standard showed better results. The detection limits were of 0.09 and 0.04 mg●L-1 for p-coumaric and ferulic acids, respectively. The proposed method showed a good linear dynamic range (3.00 - 30.00 mg●L-1) for the analytes. The usefulness of the methodology was demonstrated by addition-recovery experiments using forage samples and values were in the 83 to 99% range. The extraction of ester-linked phenolic acids by 120 minutes of ultrasound bath was faster and more reproducible than alkaline hydrolysis (20°C for 24 h).	
Palavras-Chave: 	Internal Calibration; Ultrasound Bath.	
Thesaurus Nal: 	forage; phenolic acids.	
Categoria do assunto: 	F Plantas e Produtos de Origem Vegetal	
Registro original: 	Embrapa Gado de Leite (CNPGL)	
Exemplares: 	CNPGL - (18666-1) - UPC - AP - DD -  -  -  - DIS