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Registros recuperados : 22 | |
3. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | MASSOLA JUNIOR, N. S.; PULCINELLI, C. E.; JESUS JUNIOR, W. C.; GODOY, C. V. Doenças do fumo (Nicotiana tabacum). In: KIMATI, H.; AMORIM, L.; REZENDE, J. A. M.; BERGAMIM FILHO, A.; CAMARGO, L. E. A. (Ed.). Manual de fitopatologia. 4. ed. São Paulo: Agonômica Ceres, 2005. p. 361-371. Biblioteca(s): Embrapa Soja. |
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6. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | TOZZE JÚNIOR, H. J.; MELLO, M. B. A.; MASSOLA JÚNIOR, N. S. Caracterização morfológica e fisiológica de isolados de Colletotrichum sp. causadores de antracnose em solanáceas. Summa Phytopatologica, Botucatu, v. 32, n. 1, p. 71-79, Jan./Mar. 2006. Ver errata do artigo de: Summa Phytopathologica, Botucatu, v. 32, n.2, p. 199, 2006. Biblioteca(s): Embrapa Agricultura Digital. |
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10. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | GARCIA, E. O.; CASAGRANDE, M. V.; RAGO, A. M.; MASSOLA JUNIOR, N. S. Preservação de urediniósporos de Puccinia melanocephala, agente causal de ferrugem em cana-de-açúcar. Summa Phytopathologica, Botucatu, v. 33, n. 2, p.152-156, Apr./June 2007. Biblioteca(s): Embrapa Agricultura Digital. |
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12. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | RIBEIRO, L. F. C.; MELLO, A. P. A.; BEDENDO, I. P.; KITAJIMA, E. W.; MASSOLA JÚNIOR, N. S. Ocorrência de um fitoplasma do grupo 16SrIII associado ao enfezamento em melão de São Caetano (Momordica charantia L.) no Estado de São Paulo. Summa Phytopathologica, Piracicaba, v. 30, n. 3, p. 391-393, jul./set. 2004. Biblioteca(s): Embrapa Hortaliças. |
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13. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | FURTADO, G. Q.; ALVES, S. A. M.; CARNEIRO, L. C.; GODOY, C. V.; MASSOLA JÚNIOR, N. S. Influência do estádio fenológico e da idade dos trifólios de soja na infecção de Phakopsora pachyrhizi. Tropical Plant Pathology, Brasília, DF, v. 34, n. 2, p. 118-122, 2009. Nota técnica. Biblioteca(s): Embrapa Soja; Embrapa Uva e Vinho. |
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14. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | FURTADO, G. Q.; ALVES, S. A. M.; GODOY, C. V.; SALATINO, M. L. F.; MASSOLA JÚNIOR, N. S. Influência da luminosidade e da camada de cera epicuticular de superfícies de folhas de soja na infecção de Phakopsora pachyrhizi. Tropical Plant Pathology, Brasília, DF, v. 34, n. 5, p. 306-312, Sept./Oct. 2009. Biblioteca(s): Embrapa Soja. |
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15. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | FURTADO, G. Q.; MORAES, S. R. G.; ALVES, S. A. M.; AMORIM, L.; MASSOLA JUNIOR, N. S. The infection of soybean leaves by Phakopsora pachyrhizi during conditions of discontinuous wetness. Journal of Phytopathology, Berlin, v. 159, n. 3, p. 165-170, mar. 2011. 159 165-170 Biblioteca(s): Embrapa Uva e Vinho. |
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16. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | TOZZE JÚNIOR, H. J.; GIORIA, R.; SUZUKI, O.; BRUNELLI, K. R.; BRAGA, R. S.; MASSOLA JÚNIOR, N. S. Natural occurrence of Colletotrichum coccodes (Walr.) Hughes causing anthracnose on pepper (capsicum annuum L.) in Brazil. Summa Phytopathologica, Botucatu, v. 33, n. 4, p. 419, out./dez. 2007. Biblioteca(s): Embrapa Hortaliças. |
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17. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | BOMFETI, C. A.; SOUZA-PACCOLA, E. A.; MASSOLA JUNIOR, N. S.; MARRIEL, I. E.; MEIRELLES, W. F.; CASELA, C. R.; PACCOLA-MEIRELLES, L. D. Localization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques. Tropical Plant Pathology, Brasília, v. 33, n. 1, p. 63-66, Jan./Feb. 2008. Biblioteca(s): Embrapa Milho e Sorgo. |
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18. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SOUZA-PACCOLA, E. A de; BOMFETI, C. A.; TANAKA, F. A. O.; MASSOLA JUNIOR, N. S.; COLAUTO, N. B.; FIGUEIREDO, J. E. F.; PACCOLA-MEIRELLES, L. D. Novel insights into the early stages of infection by oval conidia of Colletotrichum sublineolum. Scientia Agricola, Piracicaba, v. 72, n. 4, p. 351-355, July/Aug. 2015. Biblioteca(s): Embrapa Milho e Sorgo. |
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19. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | ROGERIO, F.; CIAMPI-GUILLARDI, M.; BARBIERI, M. C. G.; BRAGANÇA, C. A. D.; SEIXAS, C. D. S.; ALMEIDA, A. M. R.; MASSOLA JUNIOR, N. S. Phylogeny and variability of Colletotrichum truncatum associated with soybean anthracnose in Brazil. Journal of Applied Microbiology, v. 122, n. 2, p. 402-415, 2017. Biblioteca(s): Embrapa Soja. |
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20. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | FURTADO, G. Q.; ALVES, S. A. M.; CARNEIRO, L. C.; GODOY, C. V.; MASSOLA JUNIOR, N. S. . Q., ALVES, S. A. M., CARNEIRO, L.C., -. Influência da idade dos trifólios da soja na suscetibilidade à ferrugem asiática. Fitopatologia Brasileira, Brasília, DF, v. 30, p. S 127, ago. 2005. Suplemento. ref. 428. Edição dos Resumos do XXXVIII Congresso Brasileiro de Fitopatologia, Brasília, DF, ago. 2005. Biblioteca(s): Embrapa Soja. |
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Registros recuperados : 22 | |
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Registro Completo
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
17/06/2008 |
Data da última atualização: |
24/05/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
Internacional - A |
Autoria: |
BOMFETI, C. A.; SOUZA-PACCOLA, E. A.; MASSOLA JUNIOR, N. S.; MARRIEL, I. E.; MEIRELLES, W. F.; CASELA, C. R.; PACCOLA-MEIRELLES, L. D. |
Afiliação: |
Cleide A. Bomfeti, UEL; Edneia A. Sopuza-Paccola, UEL; Nelson S. Massola Junior, Esalq; IVANILDO EVODIO MARRIEL, CNPMS; WALTER FERNANDES MEIRELLES, CNPMS; Carlos Roberto Casela, CNPMS; Luzia D. Paccola-Meirelles, UEL. |
Título: |
Localization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Tropical Plant Pathology, Brasília, v. 33, n. 1, p. 63-66, Jan./Feb. 2008. |
DOI: |
10.1590/S1982-56762008000100010 |
Idioma: |
Inglês |
Conteúdo: |
The etiological agent of maize white spot (MWS) disease has been a subject of controversy and discussion. Initially the disease was described as Phaeosphaeria leaf spot caused by Phaeosphaeria maydis. Other authors have suggested the existence of different fungal species causing similar symptoms. Recently, a bacterium, Pantoea ananatis, was described as the causal agent of this disease. The purpose of this study was to offer additional information on the correct etiology of this disease by providing visual evidence of the presence of the bacterium in the interior of the MWS lesions by using transmission electron microscopy (TEM) and molecular techniques. The TEM allowed visualization of a large amount of bacteria in the intercellular spaces of lesions collected from both artificially and naturally infected plants. Fungal structures were not visualized in young lesions. Bacterial primers for the 16S rRNA and rpoB genes were used in PCR reactions 10 amplify DNA extracted from water-soaked (young) and necrotic lesions. The universal fungal oligonucleotide ITS4 was also included to identify the possible presence of funga! structures inside lesions. Positive PCR products from water-soaked lesions, both from naturally and artificially inoculated plants, were produced with bacterial primers, whereas no amplification was observed when ITS4 oligonucleotide was used. On the other hand, DNA amplification with ITS4 primer was observed when DNA was isolated from necrotic (old) lesions. These results reinforced previous report of P. ananatis as the primary pathogen and the hypothesis that fungal species may colonize lesions pre-established by P. ananatis. MenosThe etiological agent of maize white spot (MWS) disease has been a subject of controversy and discussion. Initially the disease was described as Phaeosphaeria leaf spot caused by Phaeosphaeria maydis. Other authors have suggested the existence of different fungal species causing similar symptoms. Recently, a bacterium, Pantoea ananatis, was described as the causal agent of this disease. The purpose of this study was to offer additional information on the correct etiology of this disease by providing visual evidence of the presence of the bacterium in the interior of the MWS lesions by using transmission electron microscopy (TEM) and molecular techniques. The TEM allowed visualization of a large amount of bacteria in the intercellular spaces of lesions collected from both artificially and naturally infected plants. Fungal structures were not visualized in young lesions. Bacterial primers for the 16S rRNA and rpoB genes were used in PCR reactions 10 amplify DNA extracted from water-soaked (young) and necrotic lesions. The universal fungal oligonucleotide ITS4 was also included to identify the possible presence of funga! structures inside lesions. Positive PCR products from water-soaked lesions, both from naturally and artificially inoculated plants, were produced with bacterial primers, whereas no amplification was observed when ITS4 oligonucleotide was used. On the other hand, DNA amplification with ITS4 primer was observed when DNA was isolated from necrotic (old) lesions. T... Mostrar Tudo |
Palavras-Chave: |
Doença bacteriana do milho; doenças foliares do milho; Maize bacterial disease; Maize leaf disease. |
Thesagro: |
Milho; Zea Mays. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32277/1/Localization-pantoea.pdf
|
Marc: |
LEADER 02604naa a2200277 a 4500 001 1491172 005 2018-05-24 008 2008 bl uuuu u00u1 u #d 024 7 $a10.1590/S1982-56762008000100010$2DOI 100 1 $aBOMFETI, C. A. 245 $aLocalization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques.$h[electronic resource] 260 $c2008 520 $aThe etiological agent of maize white spot (MWS) disease has been a subject of controversy and discussion. Initially the disease was described as Phaeosphaeria leaf spot caused by Phaeosphaeria maydis. Other authors have suggested the existence of different fungal species causing similar symptoms. Recently, a bacterium, Pantoea ananatis, was described as the causal agent of this disease. The purpose of this study was to offer additional information on the correct etiology of this disease by providing visual evidence of the presence of the bacterium in the interior of the MWS lesions by using transmission electron microscopy (TEM) and molecular techniques. The TEM allowed visualization of a large amount of bacteria in the intercellular spaces of lesions collected from both artificially and naturally infected plants. Fungal structures were not visualized in young lesions. Bacterial primers for the 16S rRNA and rpoB genes were used in PCR reactions 10 amplify DNA extracted from water-soaked (young) and necrotic lesions. The universal fungal oligonucleotide ITS4 was also included to identify the possible presence of funga! structures inside lesions. Positive PCR products from water-soaked lesions, both from naturally and artificially inoculated plants, were produced with bacterial primers, whereas no amplification was observed when ITS4 oligonucleotide was used. On the other hand, DNA amplification with ITS4 primer was observed when DNA was isolated from necrotic (old) lesions. These results reinforced previous report of P. ananatis as the primary pathogen and the hypothesis that fungal species may colonize lesions pre-established by P. ananatis. 650 $aMilho 650 $aZea Mays 653 $aDoença bacteriana do milho 653 $adoenças foliares do milho 653 $aMaize bacterial disease 653 $aMaize leaf disease 700 1 $aSOUZA-PACCOLA, E. A. 700 1 $aMASSOLA JUNIOR, N. S. 700 1 $aMARRIEL, I. E. 700 1 $aMEIRELLES, W. F. 700 1 $aCASELA, C. R. 700 1 $aPACCOLA-MEIRELLES, L. D. 773 $tTropical Plant Pathology, Brasília$gv. 33, n. 1, p. 63-66, Jan./Feb. 2008.
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Embrapa Milho e Sorgo (CNPMS) |
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