Data/hora: 13/06/2024 02:53:46

Biblioteca(s): | Embrapa Milho e Sorgo.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | Internacional - A
Data corrente: | 17/06/2008
Data da última atualização: | 24/05/2018
Autoria: | BOMFETI, C. A.; SOUZA-PACCOLA, E. A.; MASSOLA JUNIOR, N. S.; MARRIEL, I. E.; MEIRELLES, W. F.; CASELA, C. R.; PACCOLA-MEIRELLES, L. D.
Afiliação: | Cleide A. Bomfeti, UEL; Edneia A. Sopuza-Paccola, UEL; Nelson S. Massola Junior, Esalq; IVANILDO EVODIO MARRIEL, CNPMS; WALTER FERNANDES MEIRELLES, CNPMS; Carlos Roberto Casela, CNPMS; Luzia D. Paccola-Meirelles, UEL.
Título: | Localization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques.
Ano de publicação: | 2008
Fonte/Imprenta: | Tropical Plant Pathology, Brasília, v. 33, n. 1, p. 63-66, Jan./Feb. 2008.
DOI: | 10.1590/S1982-56762008000100010
Idioma: | Inglês
Conteúdo: | The etiological agent of maize white spot (MWS) disease has been a subject of controversy and discussion. Initially the disease was described as Phaeosphaeria leaf spot caused by Phaeosphaeria maydis. Other authors have suggested the existence of different fungal species causing similar symptoms. Recently, a bacterium, Pantoea ananatis, was described as the causal agent of this disease. The purpose of this study was to offer additional information on the correct etiology of this disease by providing visual evidence of the presence of the bacterium in the interior of the MWS lesions by using transmission electron microscopy (TEM) and molecular techniques. The TEM allowed visualization of a large amount of bacteria in the intercellular spaces of lesions collected from both artificially and naturally infected plants. Fungal structures were not visualized in young lesions. Bacterial primers for the 16S rRNA and rpoB genes were used in PCR reactions 10 amplify DNA extracted from water-soaked (young) and necrotic lesions. The universal fungal oligonucleotide ITS4 was also included to identify the possible presence of funga! structures inside lesions. Positive PCR products from water-soaked lesions, both from naturally and artificially inoculated plants, were produced with bacterial primers, whereas no amplification was observed when ITS4 oligonucleotide was used. On the other hand, DNA amplification with ITS4 primer was observed when DNA was isolated from necrotic (old) lesions. These results reinforced previous report of P. ananatis as the primary pathogen and the hypothesis that fungal species may colonize lesions pre-established by P. ananatis.
Palavras-Chave: | Doença bacteriana do milho; doenças foliares do milho; Maize bacterial disease; Maize leaf disease.
Thesagro: | Milho; Zea Mays.
Categoria do assunto: | H Saúde e Patologia
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/32277/1/Localization-pantoea.pdf
Registro original: | Embrapa Milho e Sorgo (CNPMS)
Exemplares: | CNPMS - (20726-1) - UPC - AP - DD -  -  -  - DIS