Data/hora: 17/06/2024 14:53:35

Biblioteca(s): | Embrapa Gado de Leite.
Data corrente: | 30/12/2019
Data da última atualização: | 06/02/2024
Autoria: | SBARDELLA, A. P.; WELLER, M. M. D. C. A.; I. FONSECA; N. B. STAFUZZA; P. A. BERNARDES; F. F. e SILVA; SILVA, M. V. G. B.; MARTINS, M. F.; D. P. MUNARI.
Afiliação: | A. P. SBARDELLA; M. M. D. C. A. WELLER; MARCOS VINICIUS GUALBERTO B SILVA, CNPGL; MARTA FONSECA MARTINS, CNPGL.
Título: | Ribonucleic acid sequencing differential gene expression analysis of isolated perfused bovine udders experimentally inoculated with Streptococcus agalactiae.
Ano de publicação: | 2019
Fonte/Imprenta: | Journal of Dairy Science, v. 102, n. 2, p. 1761-1767, 2019.
DOI: | https://doi.org/10.3168/jds.2018-15516
Idioma: | Inglês
Conteúdo: | The aim of this study was to elucidate the differential gene expression in the RNA sequencing transcriptome of isolated perfused udders collected from 4 slaughtered Holstein × Zebu crossbred dairy cows experimentally inoculated with Streptococcus agalactiae. We studied 3 different statistical tools (edgeR, baySeq, and Cuffdiff 2). In summary, 2 quarters of each udder were experimentally inoculated with Strep. agalactiae and the other 2 were used as a control. Mammary tissue biopsies were collected at times 0 and 3 h after infection. The total RNA was extracted and sequenced on an Illumina HiSeq 2000 (Illumina Inc., San Diego, CA). Transcripts were assembled from the reads aligned to the bovine UMD 3.1 reference genome, and the statistical analyses were performed using the previously mentioned tools (edgeR, baySeq, and Cuffdiff 2). Finally, the identified genes were submitted to pathway enrichment analysis. A total of 1,756, 1,161, and 3,389 genes with differential gene expression were identified when using edgeR, baySeq, and Cuffdiff 2, respectively. A total of 122 genes were identified by the overlapping of the 3 methods; however, only the platelet activation presented a significantly enriched pathway. From the results, we suggest the FCER1G, GNAI2, ORAI1, and VASP genes shared among the 3 methods in this pathway for posterior biological validation.
Palavras-Chave: | BaySeq; Crossbred dairy cow; Cuffdiff 2; EdgeR; RNA sequencing.
Categoria do assunto: | G Melhoramento Genético
Registro original: | Embrapa Gado de Leite (CNPGL)
Exemplares: | CNPGL - (24917-1) - UPC - AP - DD -  -  -  - DIS