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Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
24/11/2016 |
Data da última atualização: |
01/03/2017 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SILVEIRA, A. L.; MADARI, B. E.; OLIVEIRA, J. M. |
Afiliação: |
ANDRE LUIZ SILVEIRA; BEATA EMOKE MADARI, CNPAF; JANAÍNA M. OLIVEIRA. |
Título: |
The impact of the implementation of an integrated crop-livestock-forest system in a Ferralsol of the brazilian savannah (Cerrado). |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
In: INTERNATIONAL SYMPOSIUM ON GREENHOUSE GASES IN AGRICULTURE, 2., 2016, Campo Grande, MS. Proceedings... Brasília, DF: Embrapa, 2016. |
Páginas: |
p. 486-487. |
Série: |
(Embrapa Gado de Corte. Documentos, 216). |
Idioma: |
Inglês |
Notas: |
Coordenador Roberto Giolo de Almeida. II SIGEE. |
Conteúdo: |
This study aimed to contribute to the analysis of an iCLF system in the Brazilian savannah in Goiás state. |
Palavras-Chave: |
Integração Lavoura-Pecuária-Floresta. |
Thesagro: |
Cerrado; Ferralsolo; Solo. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/150572/1/CNPAF-2016-p486.pdf
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Marc: |
LEADER 00940nam a2200217 a 4500 001 2057084 005 2017-03-01 008 2016 bl uuuu u00u1 u #d 100 1 $aSILVEIRA, A. L. 245 $aThe impact of the implementation of an integrated crop-livestock-forest system in a Ferralsol of the brazilian savannah (Cerrado).$h[electronic resource] 260 $aIn: INTERNATIONAL SYMPOSIUM ON GREENHOUSE GASES IN AGRICULTURE, 2., 2016, Campo Grande, MS. Proceedings... Brasília, DF: Embrapa$c2016 300 $ap. 486-487. 490 $a(Embrapa Gado de Corte. Documentos, 216). 500 $aCoordenador Roberto Giolo de Almeida. II SIGEE. 520 $aThis study aimed to contribute to the analysis of an iCLF system in the Brazilian savannah in Goiás state. 650 $aCerrado 650 $aFerralsolo 650 $aSolo 653 $aIntegração Lavoura-Pecuária-Floresta 700 1 $aMADARI, B. E. 700 1 $aOLIVEIRA, J. M.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
09/05/2022 |
Data da última atualização: |
09/05/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
AMARAL, T. F.; GRAZIA, J. G. V. de; MARTINHAO, L A. G.; DE COL, F.; SIQUEIRA, L. G. B.; VIANA, J. H. M.; HANSEN, P. J. |
Afiliação: |
THIAGO F. AMARAL, University of Florida; JOAO GABRIEL VIANA DE GRAZIA, FIVX Apoyar Biotech Ltda; LUANY ALVES GALVAO MARTINHAO, FIVX Apoyar Biotech Ltda; FELIPE DE COL, FIVX Apoyar Biotech Ltda; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; JOAO HENRIQUE MOREIRA VIANA, Cenargen; PETER J. HANSEN, University of Florida. |
Título: |
Actions of CSF2 and DKK1 on bovine embryo development and pregnancy outcomes are affected by composition of embryo culture medium. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Scientific Reports, v. 12, 7503, 2022. |
DOI: |
https://doi.org/10.1038/s41598-022-11447-7 |
Idioma: |
Inglês |
Conteúdo: |
Procedures for in vitro embryo production in cattle have not been optimized. In the current experiment, we utilized a 3 × 3 factorial design to test whether the proportion of embryos becoming blastocysts in culture and the pregnancy rate after embryo transfer are afected by type of serum in the medium [no serum; 3% (v/v) KnockOut Serum Replacement (SR); 3% (v/v) fetal bovine serum (FBS)] and addition of specifc embryokines [vehicle; 10 ng/mL colony stimulating factor 2 (CSF2); 100 ng/mL dickkopf related protein 1 (DKK1)] at day 5 of culture. Embryos were produced using abattoir-derived ovaries and Y-sorted semen from two Angus sires. The percent of putative zygotes and cleaved embryos becoming blastocysts was improved by SR and FBS. Pregnancy rate at day 30 was determined for 1426 Nelore recipients and calving rate for 266 recipients. In the absence of CSF2 or DKK1, pregnancy rates were lower for embryos cultured with SR or FBS. CSF2 and DKK1 reduced pregnancy rate for embryos cultured without serum but had no detrimental efect in the SR or FBS groups. Indeed, CSF2 blocked the negative efect of FBS on pregnancy rate. Data on birth weights were available for 67 bull calves. There were no efects of treatment. The sire used to produce embryos had signifcant and large efects on development to the blastocyst stage, pregnancy rate at day 30, calving rate and pregnancy loss between day 30 and calving. Results indicate that (1) SR and FBS can improve embryonic development in vitro while also compromising competence of embryos to survive after transfer, (2) actions of CSF2 and DKK1 depend upon other characteristics of the embryo production system, and (3) sire can have a large efect on embryonic development before and after transfer. MenosProcedures for in vitro embryo production in cattle have not been optimized. In the current experiment, we utilized a 3 × 3 factorial design to test whether the proportion of embryos becoming blastocysts in culture and the pregnancy rate after embryo transfer are afected by type of serum in the medium [no serum; 3% (v/v) KnockOut Serum Replacement (SR); 3% (v/v) fetal bovine serum (FBS)] and addition of specifc embryokines [vehicle; 10 ng/mL colony stimulating factor 2 (CSF2); 100 ng/mL dickkopf related protein 1 (DKK1)] at day 5 of culture. Embryos were produced using abattoir-derived ovaries and Y-sorted semen from two Angus sires. The percent of putative zygotes and cleaved embryos becoming blastocysts was improved by SR and FBS. Pregnancy rate at day 30 was determined for 1426 Nelore recipients and calving rate for 266 recipients. In the absence of CSF2 or DKK1, pregnancy rates were lower for embryos cultured with SR or FBS. CSF2 and DKK1 reduced pregnancy rate for embryos cultured without serum but had no detrimental efect in the SR or FBS groups. Indeed, CSF2 blocked the negative efect of FBS on pregnancy rate. Data on birth weights were available for 67 bull calves. There were no efects of treatment. The sire used to produce embryos had signifcant and large efects on development to the blastocyst stage, pregnancy rate at day 30, calving rate and pregnancy loss between day 30 and calving. Results indicate that (1) SR and FBS can improve embryonic development in vitro w... Mostrar Tudo |
Palavras-Chave: |
Fertilização; Produção in vitro. |
Thesagro: |
Bovino; Embrião; Reprodução Animal. |
Thesaurus NAL: |
Animal reproduction; Embryo (animal); In vitro fertilization. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1142749/1/Actions-of-CSF2.pdf
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Marc: |
LEADER 02687naa a2200301 a 4500 001 2142749 005 2022-05-09 008 2022 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1038/s41598-022-11447-7$2DOI 100 1 $aAMARAL, T. F. 245 $aActions of CSF2 and DKK1 on bovine embryo development and pregnancy outcomes are affected by composition of embryo culture medium.$h[electronic resource] 260 $c2022 520 $aProcedures for in vitro embryo production in cattle have not been optimized. In the current experiment, we utilized a 3 × 3 factorial design to test whether the proportion of embryos becoming blastocysts in culture and the pregnancy rate after embryo transfer are afected by type of serum in the medium [no serum; 3% (v/v) KnockOut Serum Replacement (SR); 3% (v/v) fetal bovine serum (FBS)] and addition of specifc embryokines [vehicle; 10 ng/mL colony stimulating factor 2 (CSF2); 100 ng/mL dickkopf related protein 1 (DKK1)] at day 5 of culture. Embryos were produced using abattoir-derived ovaries and Y-sorted semen from two Angus sires. The percent of putative zygotes and cleaved embryos becoming blastocysts was improved by SR and FBS. Pregnancy rate at day 30 was determined for 1426 Nelore recipients and calving rate for 266 recipients. In the absence of CSF2 or DKK1, pregnancy rates were lower for embryos cultured with SR or FBS. CSF2 and DKK1 reduced pregnancy rate for embryos cultured without serum but had no detrimental efect in the SR or FBS groups. Indeed, CSF2 blocked the negative efect of FBS on pregnancy rate. Data on birth weights were available for 67 bull calves. There were no efects of treatment. The sire used to produce embryos had signifcant and large efects on development to the blastocyst stage, pregnancy rate at day 30, calving rate and pregnancy loss between day 30 and calving. Results indicate that (1) SR and FBS can improve embryonic development in vitro while also compromising competence of embryos to survive after transfer, (2) actions of CSF2 and DKK1 depend upon other characteristics of the embryo production system, and (3) sire can have a large efect on embryonic development before and after transfer. 650 $aAnimal reproduction 650 $aEmbryo (animal) 650 $aIn vitro fertilization 650 $aBovino 650 $aEmbrião 650 $aReprodução Animal 653 $aFertilização 653 $aProdução in vitro 700 1 $aGRAZIA, J. G. V. de 700 1 $aMARTINHAO, L A. G. 700 1 $aDE COL, F. 700 1 $aSIQUEIRA, L. G. B. 700 1 $aVIANA, J. H. M. 700 1 $aHANSEN, P. J. 773 $tScientific Reports$gv. 12, 7503, 2022.
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