| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Trigo. Para informações adicionais entre em contato com cnpt.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Trigo. |
|
Data corrente: |
10/01/2010 |
|
Data da última atualização: |
11/09/2012 |
|
Autoria: |
RÖDER, M. S.; PLASCHKE, J.; KÖNIG, S. U.; BÖRNER, A.; SORRELLS, M. E.; TANKSLEY, S. D.; GANAL, M. W. |
|
Afiliação: |
2-4,7 Institut fur Pflanzengenetik und Kulturpflanzenforschung, Germany, 1,5-6 Cornell University. |
|
Título: |
Abundance, variability and chromosomal location of microsatellites in wheat. |
|
Ano de publicação: |
1995 |
|
Fonte/Imprenta: |
Molecular and General Genetics, v. 246, n. 3, p. 327-333, 1995. |
|
Idioma: |
Inglês |
|
Conteúdo: |
The potential of microsatellite sequences as genetic markers in hexaploid wheat (Triticum aestivum) was investigated with respect to their abundance, variability, chromosomal location and usefulness in related species. By screening a lambda phage library, the total number of (GA)n blocks was estimated to be 3.6 x 104 and the number of (GT)n blocks to be 2.3 x 104 per haploid wheat genome. This results in an average distance of approximately 270 kb between these two microsatellite types combined. Based on sequence analysis data from 70 isolated microsatellites, it was found that wheat microsatellites are relatively long containing up to 40 dinucleotide repeats. Of the tested primer pairs, 36% resulted in fragments with a size corresponding to the expected length of the sequenced microsatellite clone. The variability of 15 microsatellite markers was investigated on 18 wheat accessions. Significantly, more variation was detected with the microsatellite markers than with RFLP markers with, on average, 4.6 different alleles per microsatellite. The 15 PCR-amplified microsatellites were further localized on chromosome arms using cytogenetic stocks of Chinese Spring. Finally, the primers for the 15 wheat microsatellites were used for PCR amplification with rye (Secale cereale) and barley accessions (Hordeum vulgare, H. spontaneum). Amplified fragments were observed for ten primer pairs with barley DNA and for nine primer pairs with rye DNA as template. A microsatellite was found by dot blot analysis in the PCR products of barley and rye DNA for only one primer pair. MenosThe potential of microsatellite sequences as genetic markers in hexaploid wheat (Triticum aestivum) was investigated with respect to their abundance, variability, chromosomal location and usefulness in related species. By screening a lambda phage library, the total number of (GA)n blocks was estimated to be 3.6 x 104 and the number of (GT)n blocks to be 2.3 x 104 per haploid wheat genome. This results in an average distance of approximately 270 kb between these two microsatellite types combined. Based on sequence analysis data from 70 isolated microsatellites, it was found that wheat microsatellites are relatively long containing up to 40 dinucleotide repeats. Of the tested primer pairs, 36% resulted in fragments with a size corresponding to the expected length of the sequenced microsatellite clone. The variability of 15 microsatellite markers was investigated on 18 wheat accessions. Significantly, more variation was detected with the microsatellite markers than with RFLP markers with, on average, 4.6 different alleles per microsatellite. The 15 PCR-amplified microsatellites were further localized on chromosome arms using cytogenetic stocks of Chinese Spring. Finally, the primers for the 15 wheat microsatellites were used for PCR amplification with rye (Secale cereale) and barley accessions (Hordeum vulgare, H. spontaneum). Amplified fragments were observed for ten primer pairs with barley DNA and for nine primer pairs with rye DNA as template. A microsatellite was found by ... Mostrar Tudo |
|
Palavras-Chave: |
Genetic marker; Marcadores genéticos; Marcadores moleculares; Microssatélite; SSR; STS (sequence tagged sites). |
|
Thesagro: |
Biotecnologia; Trigo; Triticum Aestivum. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02417naa a2200301 a 4500
001 1840246
005 2012-09-11
008 1995 bl --- 0-- u #d
100 1 $aRÖDER, M. S.
245 $aAbundance, variability and chromosomal location of microsatellites in wheat.
260 $c1995
520 $aThe potential of microsatellite sequences as genetic markers in hexaploid wheat (Triticum aestivum) was investigated with respect to their abundance, variability, chromosomal location and usefulness in related species. By screening a lambda phage library, the total number of (GA)n blocks was estimated to be 3.6 x 104 and the number of (GT)n blocks to be 2.3 x 104 per haploid wheat genome. This results in an average distance of approximately 270 kb between these two microsatellite types combined. Based on sequence analysis data from 70 isolated microsatellites, it was found that wheat microsatellites are relatively long containing up to 40 dinucleotide repeats. Of the tested primer pairs, 36% resulted in fragments with a size corresponding to the expected length of the sequenced microsatellite clone. The variability of 15 microsatellite markers was investigated on 18 wheat accessions. Significantly, more variation was detected with the microsatellite markers than with RFLP markers with, on average, 4.6 different alleles per microsatellite. The 15 PCR-amplified microsatellites were further localized on chromosome arms using cytogenetic stocks of Chinese Spring. Finally, the primers for the 15 wheat microsatellites were used for PCR amplification with rye (Secale cereale) and barley accessions (Hordeum vulgare, H. spontaneum). Amplified fragments were observed for ten primer pairs with barley DNA and for nine primer pairs with rye DNA as template. A microsatellite was found by dot blot analysis in the PCR products of barley and rye DNA for only one primer pair.
650 $aBiotecnologia
650 $aTrigo
650 $aTriticum Aestivum
653 $aGenetic marker
653 $aMarcadores genéticos
653 $aMarcadores moleculares
653 $aMicrossatélite
653 $aSSR
653 $aSTS (sequence tagged sites)
700 1 $aPLASCHKE, J.
700 1 $aKÖNIG, S. U.
700 1 $aBÖRNER, A.
700 1 $aSORRELLS, M. E.
700 1 $aTANKSLEY, S. D.
700 1 $aGANAL, M. W.
773 $tMolecular and General Genetics$gv. 246, n. 3, p. 327-333, 1995.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Trigo (CNPT) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Pantanal; Embrapa Recursos Genéticos e Biotecnologia. |
|
Data corrente: |
28/11/2018 |
|
Data da última atualização: |
30/11/2018 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
B - 3 |
|
Autoria: |
MAGNUSSON, W. E.; GRELLE, C. E. V.; MARQUES, M. C. M.; ROCHA, C. F. D.; DIAS, B.; FONTANA, C. S.; BERGALLO, H.; OVERBECK, G. E.; VALE, M. M.; TOMAS, W. M.; CERQUEIRA, R.; COLLEVATTI, R.; PILLAR, V. D.; MALABARBA, L. R.; LINS-E-SILVA, A. C.; NECKEL-OLIVEIRA, S.; MARTINELLI, B.; AKAMA, A.; RODRIGUES, D.; SILVEIRA, L. F.; SCARIOT, A. O.; FERNANDES, G. W. |
|
Afiliação: |
WILLIAM E. MAGNUSSON, INPA; CARLOS E. V. GRELLE, UFRJ; MÁRCIA C. M. MARQUES, UFPR; CARLOS F. D. ROCHA, UERJ; BRAULIO DIAS, UnB; CARLA S. FONTANA, PUCRS; HELENA BERGALLO, UERJ; GERHARD E. OVERBECK, UFRGS; MARIANA M. VALE, UFRJ; WALFRIDO MORAES TOMAS, CPAP; RUI CERQUEIRA, UFRJ; ROSANE COLLEVATTI, UFG; VALÉRIO D. PILLAR, UFRGS; LUIZ R. MALABARBA, UFRGS; ANA CAROLINA LINS-E-SILVA, UFRPE; SELVINO NECKEL-OLIVEIRA, UFSC; BRUNO MARTINELLI, UnB; ALBERTO AKAMA, MUSEU PARAENSE EMILIO GOELDI; DOMINGOS RODRIGUES, UFMT; LUIS F. SILVEIRA, USP; ALDICIR OSNI SCARIOT, Cenargen; GERALDO W. FERNANDES, UFMG. |
|
Título: |
Effects of Brazil's political crisis on the science needed for biodiversity conservation. |
|
Ano de publicação: |
2018 |
|
Fonte/Imprenta: |
Frontiers in Ecology and Evolution, v. 6, n. 163, p. 1-6, oct. 2018. |
|
DOI: |
https://doi.org/10.3389/fevo.2018.00163 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The effects of Brazil's political crisis on science funding necessary for biodiversity conservation are likely to be global. Brazil is not only the world?s most biodiverse nation, it is responsible for the greater part of the Amazon forest, which regulates the climate and provides rain to much of southern South America. Brazil was a world leader in satellite monitoring of land-use change, in-situ biodiversity monitoring, reduction in tropical-forest deforestation, protection of indigenous lands, and a model for other developing nations. Coordinated public responses will be necessary to prevent special-interest groups from using the political crisis to weaken science funding, environmental legislation and law enforcement. |
|
Thesagro: |
Biodiversidade; Financiamento; Pesquisa. |
|
Thesaurus NAL: |
Biodiversity; Brazil; Climate change; Funding; Governance; Research. |
|
Categoria do assunto: |
--
P Recursos Naturais, Ciências Ambientais e da Terra |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/187428/1/Magnusson-Effects-of-Brazil.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/187902/1/fevo-06-00163.pdf
|
|
Marc: |
LEADER 02095naa a2200493 a 4500
001 2100242
005 2018-11-30
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3389/fevo.2018.00163$2DOI
100 1 $aMAGNUSSON, W. E.
245 $aEffects of Brazil's political crisis on the science needed for biodiversity conservation.$h[electronic resource]
260 $c2018
520 $aThe effects of Brazil's political crisis on science funding necessary for biodiversity conservation are likely to be global. Brazil is not only the world?s most biodiverse nation, it is responsible for the greater part of the Amazon forest, which regulates the climate and provides rain to much of southern South America. Brazil was a world leader in satellite monitoring of land-use change, in-situ biodiversity monitoring, reduction in tropical-forest deforestation, protection of indigenous lands, and a model for other developing nations. Coordinated public responses will be necessary to prevent special-interest groups from using the political crisis to weaken science funding, environmental legislation and law enforcement.
650 $aBiodiversity
650 $aBrazil
650 $aClimate change
650 $aFunding
650 $aGovernance
650 $aResearch
650 $aBiodiversidade
650 $aFinanciamento
650 $aPesquisa
700 1 $aGRELLE, C. E. V.
700 1 $aMARQUES, M. C. M.
700 1 $aROCHA, C. F. D.
700 1 $aDIAS, B.
700 1 $aFONTANA, C. S.
700 1 $aBERGALLO, H.
700 1 $aOVERBECK, G. E.
700 1 $aVALE, M. M.
700 1 $aTOMAS, W. M.
700 1 $aCERQUEIRA, R.
700 1 $aCOLLEVATTI, R.
700 1 $aPILLAR, V. D.
700 1 $aMALABARBA, L. R.
700 1 $aLINS-E-SILVA, A. C.
700 1 $aNECKEL-OLIVEIRA, S.
700 1 $aMARTINELLI, B.
700 1 $aAKAMA, A.
700 1 $aRODRIGUES, D.
700 1 $aSILVEIRA, L. F.
700 1 $aSCARIOT, A. O.
700 1 $aFERNANDES, G. W.
773 $tFrontiers in Ecology and Evolution$gv. 6, n. 163, p. 1-6, oct. 2018.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Pantanal (CPAP) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
