Data/hora: 19/06/2024 05:18:02

Biblioteca(s): | Embrapa Arroz e Feijão.
Data corrente: | 18/12/2023
Data da última atualização: | 18/12/2023
Autoria: | LEONARDO-SILVA, L.; SILVA, L. B. da; FERREIRA, E. P. de B.; REIS, K. F. D. N. S. dos; NAVES, P. L. F.; MARTIN-DIDONET, C. C. G.
Afiliação: | LUCAS LEONARDO-SILVA, UEG; LARISSA B. DA SILVA, UEG; ENDERSON PETRONIO DE BRITO FERREIRA, CNPAF; KARINA F. D. N. S. DOS REIS, UEG; PLINIO L. F. NAVES, UEG; CLAUDIA C. G. MARTIN-DIDONET, UEG.
Título: | Assessment of morphophysiological and genotypic diversity of endophytic bacteria isolated from rice (Oryza sativa L.) plants.
Ano de publicação: | 2023
Fonte/Imprenta: | Revista Caatinga, v. 36, n. 4, p. 775-784, out./dez. 2023.
ISSN: | 1983-2125
Idioma: | Português
Conteúdo: | Rice production in Brazil incurs high costs due to the significant use of agrochemicals. Some plant growth-promoting rhizobacteria (PGPR) can be used as alternative to fertilizers and phytosanitary products. Thus, the objective of this study was to characterize endophytic bacteria isolated from roots of rice plants. The isolates were characterized based on colony morphology, antibiotic resistance, carbon sources utilization, enzyme activity (catalase, amylase, protease, cellulase, and lipase), inorganic phosphate solubilization, and the 16S-23S rDNA intergenic region. Morphologically, 68% of the isolates presented a rapid growth rate, 46% presented abundant mucus production, and 77% formed viscous colonies. All isolates were resistant to nalidixic acid and 16% presented resistance to streptomycin. The majority (90%) used monosaccharides and disaccharides in carbon source assays. Most of the isolates (95%) were positive for catalase and 63.6% were positive for amylase, protease, lipase, and cellulase activities. Additionally, 59% of them were able to solubilize phosphate. The mean enzymatic index for amylase, cellulase, and protease was 2.8, 3.5, and 1.7 respectively. The similarity analysis revealed high diversity among the isolates, with similarity indices of 70% (based on morphological characteristics) and 60% (based on the intergenic region 16S-23S rDNA). Considering morphophysiological and genotypic characteristics, three promising isolates should be evaluated in studies under field conditions for the potential development of bioproducts to replace industrially manufactured inputs in rice crops.
Palavras-Chave: | Extracellular enzymes; OCR 16S-23S; RDNA.
Thesagro: | Arroz; Bactéria; Enzima; Fator de Crescimento; Oryza Sativa.
Thesaurus Nal: | Genotype; Plant growth-promoting rhizobacteria; Rice.
Categoria do assunto: | F Plantas e Produtos de Origem Vegetal
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1159886/1/rev-caatinga-2023.pdf
Registro original: | Embrapa Arroz e Feijão (CNPAF)
Exemplares: | CNPAF - (36850-1) - UPC - AP - DD - 2023 -  - 2023 - DIS