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| Registros recuperados : 109 | |
| 3. |  | MARQUES, M. G.; ZANELLA, R. O que fará a PIV de suínos chegar ao uso comercial? In: CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 24.; INTERNATIONAL SYMPOSIUM ON ANIMAL BIOLOGY OF REPRODUCTION, 8., 2021, Belo Horizonte. Anais... Belo Horizonte: CBRA, 2021. XXIV CBRA-VIII ISABR-Joint Meeting. Revista Brasileira de Reprodução Anima, v. 45, n. 4, p. 559-565, out./dez. 2021.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 8. | | MARQUES, M. G.; MAULE, L. E.; DAHMER, A.; KRAMER, B.; BRUM, J. S.; RECH, R. R. Different hormonal stimulation protocols can affect the number of ovulations, volume of corporea lutea and progesterone production in sows? In: ANNUAL MEETING OF THE BRAZILIAN EMBRYO TECHNOLOGY SOCIETY (SBTE), 28, 2014, Natal. Abstracts... Natal, 2014. Publicado em: Animal Reproduction, v. 11, n. 3, p. 304, Jul./Sept. 2014.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 11. | | ZANELLA, R.; LEDUR, M. C.; PEIXOTO, J. de O.; LÚCIO, C. F.; MARQUES, M. G. Effect of broiler chicken lines on sperm quality. In: ANNUAL MEETING OF THE BRAZILIAN EMBRYO TECHNOLOGY SOCIETY (SBTE), 28, 2014, Natal. Abstracts... Natal, 2014. Publicado em: Animal Reproduction, v. 11, n. 3, p. 304, Jul./Sept. 2014.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 19. | | SOUZA, A. P. de; SILVA, Z.; SUPERTI, B. F. V.; MARQUES, M. G. Caminhos da Biotecnologia da Reprodução em Suínos. Suinocultura Industrial, Itu, ed. 271, ano 38, n. 04, p. 18-23, 2016.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 20. | | ANSOLIN, A. L.; FACCIN, J. C.; ZANELLA, R.; BIANCHI, I.; KICH, J. D.; MARQUES, M. G. A lavagem prepucial pode reduzir a contaminação bacteriana nos ejaculados de machos suínos? Suinocultura Industrial, Itu, ed. 311, ano 45, n. 2, p. 14-17, 2023.| Biblioteca(s): Embrapa Suínos e Aves. |
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| Registros recuperados : 109 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
21/12/2022 |
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Data da última atualização: |
21/12/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 3 |
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Autoria: |
RECCHIA, K.; MACHADO, L. S.; BOTIGELLI, R. C.; PIERI, N. C. G.; BARBOSA, G.; CASTRO, R. V. G. de; MARQUES, M. G.; PESSÔA, L. V. de F.; FANTINATO NETO, P.; MEIRELLES, F. V.; SOUZA, A. F. de; MARTINS, S. M. M. K.; BRESSAN, F. F. |
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Afiliação: |
KAIANA RECCHIA, Universidade de São Paulo; LUCAS SIMÕES MACHADO, Universidade de São Paulo; RAMON CESAR BOTIGELLI, Universidade Estadual Paulista; NAIRA CAROLINE GODOY PIERI, Universidade de São Paulo; GABRIELA BARBOSA, Universidade de São Paulo; RAQUEL VASCONCELOS GUIMARÃES DE CASTRO, Universidade de São Paulo; MARIANA GROKE MARQUES, CNPSA; LAÍS VICARI DE FIGUEIREDO PESSÔA, Universidade de São Paulo; PAULO FANTINATO NETO, Universidade de São Paulo; FLÁVIO VIEIRA MEIRELLES, Universidade de São Paulo; ALINE FERNANDA DE SOUZA, Universidade de São Paulo; SIMONE MARIA MASSAMI KITAMURA MARTINS, Universidade de São Paulo; FABIANA FERNANDES BRESSAN, Universidade de São Paulo. |
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Título: |
In vitro induced pluripotency from urine-derived cells in porcine. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
World Journal of Stem Cells, v. 14, n. 3, p. 231-244, 2022. |
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DOI: |
https://doi.org/10.4252/wjsc.v14.i3.231 |
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Idioma: |
Inglês |
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Conteúdo: |
Methods: The UDCs were reprogrammed in vitro using human or murine octamer-binding transcription factor 4 (OCT4), SRY-box2 (SOX2), Kruppel-like factor 4 (KLF4), and C-MYC, and cultured with basic fibroblast growth factor (bFGF) supplementation. To characterize the putative porcine iPSCs three clonal lineages were submitted to immunocytochemistry for alkaline phosphatase (AP), OCT4, SOX2, NANOG, TRA1 81 and SSEA 1 detection. Endogenous transcripts related to the pluripotency (OCT4, SOX2 and NANOG) were analyzed via reverse transcription quantitative real-time polymerase chain reaction in different time points during the culture, and all three lineages formed embryoid bodies (EBs) when cultured in suspension without bFGF supplementation. Results: The UDCs were isolated from swine urine samples and when at passage 2 submitted to in vitro reprogramming. Colonies of putative iPSCs were obtained only from UDCs transduced with the murine factors (mOSKM), but not from human factors (hOSKM). Three clonal lineages were isolated and further cultured for at least 28 passages, all the lineages were positive for AP detection, the OCT4, SOX2, NANOG markers, albeit the immunocytochemical analysis also revealed heterogeneous phenotypic profiles among lineages and passages for NANOG and SSEA1, similar results were observed in the abundance of the endogenous transcripts related to pluripotent state. All the clonal lineages when cultured in suspension without bFGF were able to form EBs expressing ectoderm and mesoderm layers transcripts. Conclusion: For the first time UDCs were isolated in the swine model and reprogrammed into a pluripotent-like state, enabling new numerous applications in both human or veterinary regenerative medicine. MenosMethods: The UDCs were reprogrammed in vitro using human or murine octamer-binding transcription factor 4 (OCT4), SRY-box2 (SOX2), Kruppel-like factor 4 (KLF4), and C-MYC, and cultured with basic fibroblast growth factor (bFGF) supplementation. To characterize the putative porcine iPSCs three clonal lineages were submitted to immunocytochemistry for alkaline phosphatase (AP), OCT4, SOX2, NANOG, TRA1 81 and SSEA 1 detection. Endogenous transcripts related to the pluripotency (OCT4, SOX2 and NANOG) were analyzed via reverse transcription quantitative real-time polymerase chain reaction in different time points during the culture, and all three lineages formed embryoid bodies (EBs) when cultured in suspension without bFGF supplementation. Results: The UDCs were isolated from swine urine samples and when at passage 2 submitted to in vitro reprogramming. Colonies of putative iPSCs were obtained only from UDCs transduced with the murine factors (mOSKM), but not from human factors (hOSKM). Three clonal lineages were isolated and further cultured for at least 28 passages, all the lineages were positive for AP detection, the OCT4, SOX2, NANOG markers, albeit the immunocytochemical analysis also revealed heterogeneous phenotypic profiles among lineages and passages for NANOG and SSEA1, similar results were observed in the abundance of the endogenous transcripts related to pluripotent state. All the clonal lineages when cultured in suspension without bFGF were able to form EBs expressi... Mostrar Tudo |
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Palavras-Chave: |
Células-tronco; IPSC; Noninvasive; Pluripotência; Pluripotency; Reprogramming. |
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Thesagro: |
Suíno; Urina. |
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Thesaurus NAL: |
Induced pluripotent stem cells; Swine; Urine. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1150134/1/10084.pdf
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Marc: |
LEADER 02880naa a2200409 a 4500
001 2150134
005 2022-12-21
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.4252/wjsc.v14.i3.231$2DOI
100 1 $aRECCHIA, K.
245 $aIn vitro induced pluripotency from urine-derived cells in porcine.$h[electronic resource]
260 $c2022
520 $aMethods: The UDCs were reprogrammed in vitro using human or murine octamer-binding transcription factor 4 (OCT4), SRY-box2 (SOX2), Kruppel-like factor 4 (KLF4), and C-MYC, and cultured with basic fibroblast growth factor (bFGF) supplementation. To characterize the putative porcine iPSCs three clonal lineages were submitted to immunocytochemistry for alkaline phosphatase (AP), OCT4, SOX2, NANOG, TRA1 81 and SSEA 1 detection. Endogenous transcripts related to the pluripotency (OCT4, SOX2 and NANOG) were analyzed via reverse transcription quantitative real-time polymerase chain reaction in different time points during the culture, and all three lineages formed embryoid bodies (EBs) when cultured in suspension without bFGF supplementation. Results: The UDCs were isolated from swine urine samples and when at passage 2 submitted to in vitro reprogramming. Colonies of putative iPSCs were obtained only from UDCs transduced with the murine factors (mOSKM), but not from human factors (hOSKM). Three clonal lineages were isolated and further cultured for at least 28 passages, all the lineages were positive for AP detection, the OCT4, SOX2, NANOG markers, albeit the immunocytochemical analysis also revealed heterogeneous phenotypic profiles among lineages and passages for NANOG and SSEA1, similar results were observed in the abundance of the endogenous transcripts related to pluripotent state. All the clonal lineages when cultured in suspension without bFGF were able to form EBs expressing ectoderm and mesoderm layers transcripts. Conclusion: For the first time UDCs were isolated in the swine model and reprogrammed into a pluripotent-like state, enabling new numerous applications in both human or veterinary regenerative medicine.
650 $aInduced pluripotent stem cells
650 $aSwine
650 $aUrine
650 $aSuíno
650 $aUrina
653 $aCélulas-tronco
653 $aIPSC
653 $aNoninvasive
653 $aPluripotência
653 $aPluripotency
653 $aReprogramming
700 1 $aMACHADO, L. S.
700 1 $aBOTIGELLI, R. C.
700 1 $aPIERI, N. C. G.
700 1 $aBARBOSA, G.
700 1 $aCASTRO, R. V. G. de
700 1 $aMARQUES, M. G.
700 1 $aPESSÔA, L. V. de F.
700 1 $aFANTINATO NETO, P.
700 1 $aMEIRELLES, F. V.
700 1 $aSOUZA, A. F. de
700 1 $aMARTINS, S. M. M. K.
700 1 $aBRESSAN, F. F.
773 $tWorld Journal of Stem Cells$gv. 14, n. 3, p. 231-244, 2022.
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