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Registros recuperados : 225 | |
61. | | ALMEIDA, A. M. R.; PIUGA, F. F.; MARIN, S. R. R.; KITAJIMA, E. W.; GASPAR, J. O.; OLIVEIRA, T. G. de; MORAES, T. G. de. Detection and partial characterization of a carlavirus causing stem necrosis of soybean in Brazil. Fitopatologia Brasileira, Brasília, v. 30, n. 2, p. 191-194, mar./abr. 2005. Biblioteca(s): Embrapa Soja. |
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62. | | ALMEIDA, A. M. R.; BINNECK, E.; PIUGA, F. F.; MARIN, S. R. R.; VALLE, P. R. Z. R. do; SILVEIRA, C. A. Characterization of powdery mildews strains from soybean, bean, sunflower, and weeds in Brazil using rDNA-ITS sequences. Tropical Plant Pathology, Brasília, DF, v. 33, n. 1, p. 20-26. jan./fev. 2008. Biblioteca(s): Embrapa Soja. |
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63. | | BARBOSA, D. de A.; MOLINARI, M. D. C.; FUGANTI-PAGLIARINI, R.; KAFER, J. M.; MARIN, D. C.; MARIN, S. R. R.; MERTZ-HENNING, L. M.; NEPOMUCENO, A. L. Caracterização in silico de genes drip em Glycine max e Glycine soja. In: CONGRESSO BRASILEIRO DE SOJA, 9., 2022, Foz do iguaçu, PR. Desafios para a produtividade sustentável no Mercosul: resumos. Brasília, DF: Embrapa, 2022. Regina Maria Villas Bôas de Campos Leite, Adeney de Freitas Bueno, editores técnicos. resumo 144. p. 164. Biblioteca(s): Embrapa Soja. |
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64. | | LEITE, J. P.; BARBOSA, E. G. G; MARIN, S. R. R.; MARINHO, J. P.; FUGANTI-PAGLIARINI, R.; YAMAGUCHI-SHINOZAKI, K.; NEPOMUCENO, A. L.; DESIDÉRIO, J. A. Caracterização de plantas transgênicas de soja obtidas com a forma constitutiva do fator de transcrição AREB1. In: CONGRESSO BRASILEIRO DE SOJA, 6., 2012, Cuiabá. Soja: integração nacional e desenvolvimento sustentável: resumos. Brasília, DF: Embrapa, 2012. p. 14, res. 6. Biblioteca(s): Embrapa Soja. |
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65. | | LEITE, J. P.; BARBOSA, E. G. G; MARIN, S. R. R.; MARINHO, J. P.; FUGANTI-PAGLIARINI, R.; YAMAGUCHI-SHINOZAKI, K.; NEPOMUCENO, A. L.; DESIDÉRIO, J. A. Caracterização de plantas transgênicas de soja obtidas com a forma constitutiva do fator de transcrição AREB1. In: CONGRESSO BRASILEIRO DE SOJA, 6., 2012, Cuiabá. Soja: integração nacional e desenvolvimento sustentável: anais. Brasília, DF: Embrapa, 2012. 5 p. 1 CD-ROM. Biblioteca(s): Embrapa Soja. |
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66. | | ARIAS, C. A. A.; BONATO, A. L. V.; MARIN, S. R. R.; MOREIRA, M. A.; ALMEIDA, L. A. de; CATTELI, L. L. Caracterização do germoplasma ativo de soja com marcadores moleculares tipo AFLP e Microssatélites (04.2000.322-03). In: HOFFMANN-CAMPO, C. B.; SARAIVA, O. F. (Org.). Resultados de pesquisa da Embrapa Soja - 2002: ecofisiologia e biologia molecular. Londrina: Embrapa Soja, 2003. p. 27-31. (Embrapa Soja. Documentos, 217). Biblioteca(s): Embrapa Soja. |
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67. | | ARIAS, C. A. A.; BONATO, A. L. V.; MARIN, S. R. R.; MOREIRA, M. A.; ALMEIDA, L. A. de; CATTELI, L. L. Caracterização do germoplasma ativo de soja com marcadores moleculares tipo AFLP e microssatélites. In: HOFFMANN-CAMPO, C. B.; SARAIVA, O. F. (Org.). Resultados de pesquisa da Embrapa Soja - 2001: ecofisiologia e biologia molecular. Londrina: Embrapa Soja, 2002. p. 36-37. (Embrapa Soja. Documentos, 198). Biblioteca(s): Embrapa Soja. |
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68. | | SANTIAGO, D. C.; HOMECHIN, M.; SILVA, J. F. V.; ALMEIDA, A. M. R.; CARVALHO, V. de P.; MARIN, S. R. R.; SILVEIRA, C. A. da. Diversidade genética de isolados brasileiros de Paecilomyces lilacinus revelada por RAPD. Fitopatologia Brasileira, Brasília, v. 29, p. S204, ago. 2004. Suplemento. Resumo apresentado no XXXVII Congresso Brasileiro de Fitopatologia, Gramado, RS, agosto, 2004. Biblioteca(s): Embrapa Soja. |
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69. | | NUNES, C. L.; BINNECK, E.; VIEIRA, N. D.; MEYER, M. C.; MARIN, S. R. R.; MITUTI, T.; ALMEIDA, A. M. R. Diversidade genética e patogenicidade de isolados de Myrothecium spp. In: CONGRESSO BRASILEIRO DE SOJA, 4., 2006, Londrina. Resumos... Londrina: Embrapa Soja, 2006. p. 96. Organizado por Odilon Ferreira Saraiva, Simone Ery Grosskopf. Biblioteca(s): Embrapa Soja. |
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70. | | KOLTUN, A.; SILVA, N. V. e; ANGELOTTI-MENDONÇA, J.; MARIN, S. R. R.; GONÇALVES, L. S. A.; NEPOMUCENO, A. L.; MERTZ-HENNING, L. M. CRISPR-transient expression in soybean for simplified gRNA screening in planta. Pesquisa Agropecuária Brasileira, v. 58, e03000, 2023. Título em português: Expressão transiente de CRISPR em soja para triagem simplificada de gRNA na planta. Biblioteca(s): Embrapa Soja; Embrapa Unidades Centrais. |
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71. | | SOLDERA, M. C. A.; LAURINDO, D. G.; BALESTRI, M. R. D.; MORAES, A. M.; MARIN, S. R. R.; BINNEK, E.; COSTAMILAN, L. M. Diversidade genética do complexo Diaporthe/Phomopsis em soja, no Brasil. Fitopatologia Brasileira, Brasília, DF, v. 32, p. S197, ago. 2007. Suplemento, ref. 0436. Edição dos Resumos do XL Congresso Brasileiro de Fitopatologia, Maringá, ago. 2007. Biblioteca(s): Embrapa Trigo. |
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72. | | SOLDERA, M. C. A. VIEIRA, N. D.; LAURINDO, D. G.; MORALES, A. M.; MARIN, S. R. R.; BINNEK, E.; ALMEIDA, A. M. R. Diversidade genética do complexo diaporthe/phomopsis em soja, no Brasil. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 2008, Londrina. Resumos expandidos. ..Londrina: Embrapa Soja, 2008. p. 190-194 (Embrapa Soja. Documentos, 297). Biblioteca(s): Embrapa Soja. |
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73. | | PEDROSO, J. C.; NEPOMUCENO, A. L.; SILVA, J. F. V.; CARNEIRO, N. P.; MARTINS, P. K.; BRETON, M. C.; MARIN, S. R. R.; BINNECK, E. Differential gene expression in nematode resistant soybean genotypes. Nematology, Boston, v. 4, n. 2, p. 235, 2002. Presented in Fourth International Congress of Nematology. Programme and abstracts. 8-13 June 2002, Tenbel, La Galletas, Arona, Tenerife, Canary Islands, Spain. Biblioteca(s): Embrapa Soja. |
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74. | | ROLLA, A. A. P.; BENEVENTI, M. A.; FUGANTI, R.; MARIN, S. R. R.; FARIAS, J. R. B.; ABDELNOOR, R. V.; NEPOMUCENO, A. L.; MARCELINO, F. C. Method validation for quantification of transgene copy number in soybean genome using qPCR relative quantification by 2 -^^ Ct or 2 ^ Ct formule. In: WORLD SOYBEAN RESEARCH CONFERENCE, 8., 2009, Beijing. Developing a global soy blueprint for a safe secure and sustainable supply: abstracts. Beijing: Chinese Academy of Agricultural Sciences: Institute of Crop Science, 2009. p. 233, ref. P581. WSRC 2009. Editado por Lijuan Qiu, Rongxia Guan, Jian Jin, Qijan Song, Shuntang Guo, Wenbin Li, Yuanchao Wang, Tianfu Han, Xiaobing Liu, Deyue Yu, Lianzhou Jiang, Deliang Peng. Biblioteca(s): Embrapa Soja. |
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75. | | MARINHO, J. P.; COUTINHO, I. D.; LAMEIRO, R. F.; MARIN, S. R. R.; COLNAGO, L. A.; NAKASHIMA, K.; YAMAGUCH-SHINOZAKI, K.; NEPOMUCENO, A. L.; MERTZ-HENNING, L. M. Metabolic alterations in conventional and genetically modified soybean plants with GmDREB2A;2 FL and GmDREB2A;2 CA transcription factors during water deficit. Plant Physiology and Biochemistry v. 140, 2019. 122-135 Biblioteca(s): Embrapa Instrumentação; Embrapa Soja. |
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76. | | FUGANTI, R.; BENEVENTI, M. A.; SILVA, J. F. V.; ARIAS, C. A. A.; MARIN, S. R. R.; BINNECK, E.; NEPOMUCENO, A. L. Microsatellite insertion in a heat-shock protein-promoter region (Gmhsp 17.6-L) in soybean plants resistant and susceptible to Meloidogyne javanica. In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. p. 321. (Embrapa Soja. Documentos, 228). Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. Biblioteca(s): Embrapa Soja. |
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77. | | BENEVENTI, M. A.; FUGANTI, R.; SILVA, J. F. V.; ARIAS, C. A. A.; MARIN, S. R. R.; BINNECK, E.; LIMA, C. G.; NEPOMUCENO, A. L. Microsatellite molecular markers associated with selection process of soybean resistant genotypes to Meloidogyne javanica and Heterodera glycines. In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. p. 321-322. (Embrapa Soja. Documentos, 228). Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. Biblioteca(s): Embrapa Soja. |
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78. | | ZIOBER, I. L.; PAIÃO, F. G.; MARIN, S. R. R.; MARCHI, D. F.; BINNECK, E.; NEPOMUCENO, A. L.; COUTINHO, L. L.; SHIMOKOMAKI, M. Molecular cloning of ?RYR hotspot region 1 from broiler chicken. Brazilian Archives of Biology and Technology, Curitiba, v. 52, Special number, p. 225-231, nov. 2009. Biblioteca(s): Embrapa Soja. |
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79. | | BROGIN, R. L.; ARIAS, C. A. A.; VELLO, N. A.; TOLEDO, J. F. F. de; PÍPOLO, A. E.; CATELLI, L. L.; MARIN, S. R. R. Molecular mapping of a gene conferring resistance to soybean rust. In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. p. 318. (Embrapa Soja. Documentos, 228). Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. Biblioteca(s): Embrapa Soja. |
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80. | | REIS, R. R.; NAKAYAMA, T.; MARIN, S. R. R.; MARINHO, J. P.; MARCOLINO-GOMES, J.; MERTZ-HENNING, L. M.; MOLINARI, H. B. C.; NEPOMUCENO, A. L. Engenharia genética de precisão para tolerância a seca em soja. In: CONGRESSO BRASILEIRO DE SOJA, 7.; MERCOSOJA, 2015, Florianópolis. Tecnologia e mercado global: perspectivas para soja: anais. Londrina: Embrapa Soja, 2015. 3 p. 1 CD-ROM. Biblioteca(s): Embrapa Soja. |
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Registros recuperados : 225 | |
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Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
07/04/2004 |
Data da última atualização: |
27/07/2007 |
Autoria: |
FUGANTI, R.; BENEVENTI, M. A.; SILVA, J. F. V.; ARIAS, C. A. A.; MARIN, S. R. R.; BINNECK, E.; NEPOMUCENO, A. L. |
Título: |
Microsatellite insertion in a heat-shock protein-promoter region (Gmhsp 17.6-L) in soybean plants resistant and susceptible to Meloidogyne javanica. |
Ano de publicação: |
2004 |
Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. |
Páginas: |
p. 321. |
Série: |
(Embrapa Soja. Documentos, 228).
|
Idioma: |
Inglês |
Notas: |
Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.
|
Conteúdo: |
It is estimated that 11% of annual global losses in production of soybean [Glycine max (L.) Merr.] is caused by nematode parasitism. In Brazil, nematode species in the genus Meloidogyne represent a serious yield constraint in many regions. Control measures, such as the development of resistant cultivars, are essential for the maintenance of acceptable productivity levels. Therefore, with the objective of identifying genetic polymorphisms between resistant and susceptible genotypes to the root knot nematode, Meloidogyne javanica, a population of twenty-five highly resistant lines and twenty-six highly susceptible lines, derived from a cross between resistant (PI 595099) and susceptible (BRS 133) genotypes were tested for microsatellite markers (SSR). Significant polymorphic markers were cloned and sequenced in an attempt to identify possible genomic regions responsible for the reaction to the nematode. Among 97 loci initially analyzed, 21 were polymorphic between the parents and seven showed high differential frequency (P<0,10) into the resistant population. In the F linkage group, where other quantitative trait loci (QTLs) conferring resistance to nematodes have already been reported; the SSR loci 176 Soy HSP, Satt 114 and Satt 423 showed significant correlation with the number of galls observed on soybean roots. The QTL analysis in this group showed the presence of at least one gene located next to the 176 Soy HSP marker, with a Lod of 27.5. The resulting fragment of this marker amplification was cloned, sequenced, and checked for homology by using the NCBI Blast. The analyzed sequence showed 100% homology with the promoter region of a heat-shock protein (HSP) found in soybean, Gmhsp17.6-L (M11317). Primers were designed using the entire gene sequence available in the GenBank, with the objective of generating bands in both resistant and susceptible genotypes. All resulting fragments were sequenced and showed homology with the promoter region of the Gmhsp17.6-L gene. We observed a microsatellite region (ATn repetitions) inside the promoter region of this gene, but the number of repetitions was different in each genotype tested, suggesting a possible correlation between the insertion length in the promoter and resistance or susceptibility to the nematode. After that, aimingto check the expression of this gene, a RPA (Ribonuclease Protection Assay) was performed. The results showed that both resistant and susceptible genotypes seemed to express the Gmhsp 17.6-L gene. However, the length of the microsatellite insertion in the promoter region might alter the levels of expression. MenosIt is estimated that 11% of annual global losses in production of soybean [Glycine max (L.) Merr.] is caused by nematode parasitism. In Brazil, nematode species in the genus Meloidogyne represent a serious yield constraint in many regions. Control measures, such as the development of resistant cultivars, are essential for the maintenance of acceptable productivity levels. Therefore, with the objective of identifying genetic polymorphisms between resistant and susceptible genotypes to the root knot nematode, Meloidogyne javanica, a population of twenty-five highly resistant lines and twenty-six highly susceptible lines, derived from a cross between resistant (PI 595099) and susceptible (BRS 133) genotypes were tested for microsatellite markers (SSR). Significant polymorphic markers were cloned and sequenced in an attempt to identify possible genomic regions responsible for the reaction to the nematode. Among 97 loci initially analyzed, 21 were polymorphic between the parents and seven showed high differential frequency (P<0,10) into the resistant population. In the F linkage group, where other quantitative trait loci (QTLs) conferring resistance to nematodes have already been reported; the SSR loci 176 Soy HSP, Satt 114 and Satt 423 showed significant correlation with the number of galls observed on soybean roots. The QTL analysis in this group showed the presence of at least one gene located next to the 176 Soy HSP marker, with a Lod of 27.5. The resulting fragment of this m... Mostrar Tudo |
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LEADER 03691naa a2200229 a 4500 001 1466852 005 2007-07-27 008 2004 bl uuuu u00u1 u #d 100 1 $aFUGANTI, R. 245 $aMicrosatellite insertion in a heat-shock protein-promoter region (Gmhsp 17.6-L) in soybean plants resistant and susceptible to Meloidogyne javanica. 260 $c2004 300 $ap. 321. 490 $a(Embrapa Soja. Documentos, 228). 500 $aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. 520 $aIt is estimated that 11% of annual global losses in production of soybean [Glycine max (L.) Merr.] is caused by nematode parasitism. In Brazil, nematode species in the genus Meloidogyne represent a serious yield constraint in many regions. Control measures, such as the development of resistant cultivars, are essential for the maintenance of acceptable productivity levels. Therefore, with the objective of identifying genetic polymorphisms between resistant and susceptible genotypes to the root knot nematode, Meloidogyne javanica, a population of twenty-five highly resistant lines and twenty-six highly susceptible lines, derived from a cross between resistant (PI 595099) and susceptible (BRS 133) genotypes were tested for microsatellite markers (SSR). Significant polymorphic markers were cloned and sequenced in an attempt to identify possible genomic regions responsible for the reaction to the nematode. Among 97 loci initially analyzed, 21 were polymorphic between the parents and seven showed high differential frequency (P<0,10) into the resistant population. In the F linkage group, where other quantitative trait loci (QTLs) conferring resistance to nematodes have already been reported; the SSR loci 176 Soy HSP, Satt 114 and Satt 423 showed significant correlation with the number of galls observed on soybean roots. The QTL analysis in this group showed the presence of at least one gene located next to the 176 Soy HSP marker, with a Lod of 27.5. The resulting fragment of this marker amplification was cloned, sequenced, and checked for homology by using the NCBI Blast. The analyzed sequence showed 100% homology with the promoter region of a heat-shock protein (HSP) found in soybean, Gmhsp17.6-L (M11317). Primers were designed using the entire gene sequence available in the GenBank, with the objective of generating bands in both resistant and susceptible genotypes. All resulting fragments were sequenced and showed homology with the promoter region of the Gmhsp17.6-L gene. We observed a microsatellite region (ATn repetitions) inside the promoter region of this gene, but the number of repetitions was different in each genotype tested, suggesting a possible correlation between the insertion length in the promoter and resistance or susceptibility to the nematode. After that, aimingto check the expression of this gene, a RPA (Ribonuclease Protection Assay) was performed. The results showed that both resistant and susceptible genotypes seemed to express the Gmhsp 17.6-L gene. However, the length of the microsatellite insertion in the promoter region might alter the levels of expression. 700 1 $aBENEVENTI, M. A. 700 1 $aSILVA, J. F. V. 700 1 $aARIAS, C. A. A. 700 1 $aMARIN, S. R. R. 700 1 $aBINNECK, E. 700 1 $aNEPOMUCENO, A. L. 773 $tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.
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