Data/hora: 05/06/2024 04:49:50

Biblioteca(s): | Embrapa Agroenergia; Embrapa Soja.
Tipo da produção científica: | Capítulo em Livro Técnico-Científico
Data corrente: | 18/12/2020
Data da última atualização: | 26/02/2021
Autoria: | FUGANTI-PAGLIARINI, R.; MARIN, S. R. R.; MOLINARI, M. D. C.; BARBOSA, D. de A.; MOLINARI, H. B. C.; MERTZ-HENNING, L. M.; NEUMAIER, N.; FARIAS, J. R. B.; NAKASHIMA, K.; YAMAGUCHI-SHINOZAKI, K.; NEPOMUCENO, A. L.
Afiliação: | Renata Fuganti-Pagliarini, National Council for Scientific and Technological Development (CNPq).; SILVANA REGINA ROCKENBACH MARIN, CNPSO; Mayla Daiane Côrrea Molinari, Universidade Estadual de Londrina, UEL, Londrina, PR.; Daniel de Amorim Barbosa, Universidade Estadual de Londrina, UEL, Londrina, PR.; HUGO BRUNO CORREA MOLINARI, CNPAE; LILIANE MARCIA MERTZ HENNING, CNPSO; NORMAN NEUMAIER, CNPSO; JOSE RENATO BOUCAS FARIAS, CNPSO; Kazuo Nakashima, Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki 305-8686, Japan; Kazuko Yamaguchi-Shinozaki, Research Institute for Agricultural and Life Sciences, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan; ALEXANDRE LIMA NEPOMUCENO, CNPSO.
Título: | Drought-tolerant soybean development: evaluation of GM lines under greenhouse and field conditions.
Ano de publicação: | 2020
Fonte/Imprenta: | In: NAKASHIMA, K.; URAO, Takeshi. (Ed.). Development of biotechnologies and biotech crops for stable food production under adverse environments and changing climate conditions. Tsukuba: JIRCAS, 2020. p. 57-88. (JIRCAS Working Report, 91).
Idioma: | Inglês
Conteúdo: | Drought is one of the greatest sources of environmental stress that has resulted in both economic and yield losses in many soybean-producing regions. The use of biotechnological tools aimed to produce plants with increased drought tolerance and productivity is the result of major investments in scientific and technological research. During the last decades, transcription factors (TFs) and key genes of important drought-responsive pathways have been used to develop genetically modified (GM) plants with increased tolerance to abiotic stress. To develop soybean lines with improved drought tolerance, genes encoding dehydration-responsive element binding protein (DREB) TFs and ABA-responsive element-binding proteins (AREB) as well as the GolS and NCED genes that encode the enzyme galactinol synthase (GolS, EC 2.4.1.123) of the raffinose family of oligosaccharides (RFOs) and the key enzyme in abscisic acid (ABA) biosynthesis 9-cisepoxycarotenoid dioxygenase (NCED, EC 1.13.11.51), respectively, were successfully introduced in soybean plants. After transformation, it was imperative to characterize the resultant drought tolerance of the GM lines. Thus, the soybean GM lines containing genetic constructions to overexpress AtDREB1A, GmDREB1A, AtDREB2A, AtAREB1, AtGolS2, and AtNCED3 were phenotyped based on molecular and agronomical traits, growth parameters, and survival rates under water deficit conditions in experiments carried out under greenhouse and field conditions for more than one crop season. All obtained results were compiled and are presented in this report. Overall, the GM lines are promising and show improved drought tolerance as diverse defense mechanisms aimed at surviving periods of water scarcity were targeted while retaining the productivity of the crop. These outcomes highlight soybean drought-tolerance pathways and indicate that the use of biotechnological tools in agricultural research can help producers minimize both yield and financial losses during drought-stricken crop seasons.
Palavras-Chave: | 9-cisepoxycarotenoid dioxygenase; ABA; AREB; DREB; Galactinol synthase.
Thesagro: | Deficiência Hídrica; Glycine Max; Produção; Soja.
Thesaurus Nal: | Soil water deficit; Transcription factors; Yields.
Categoria do assunto: | F Plantas e Produtos de Origem Vegetal
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/219437/1/Drought-tolerant-soybean-development-evaluation-of-GM-lines-under.pdf
Registro original: | Embrapa Soja (CNPSO)

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