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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
13/04/2011 |
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Data da última atualização: |
13/04/2011 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
FRANCO, O. L.; ANDRADE, A. E.; BARROS, E. V. S. A.; SA, M. F. G. de; CARNEIRO, R. M. D. G.; CARNEIRO, R.; EIRA, M. T. S. da; ROCHA, T. L.; REIS, A. M. dos. |
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Afiliação: |
UNIVERSIDADE CATÓLICA DE BRASÍLIA; MARIA FATIMA GROSSI DE SA, CENARGEN; REGINA MARIA DECHECHI G CARNEIRO, CENARGEN; IAPAR; MIRIAN THEREZINHA SOUZA DA EIRA, SAPC; THALES LIMA ROCHA, CENARGEN; ANGELA MEHTA DOS REIS, CENARGEN. |
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Título: |
Comparison of different staining methods for coffee proteomic analysis. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
In:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2007. 1 CD-ROM. |
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Idioma: |
Inglês |
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Conteúdo: |
Proteomic methods, such as bidimensional electrophoresis (2-DE) and mass spectrometry, have been extensively used for the study of protein differential expression in several plants including Arabidopsis thaliana, rice and wheat. Specifically in the 2-DE method, deep attention must be given to the protein staining technique, which often involves silver nitrate or Coomassie Brilliant Blue (CBB). Silver staining is usually preferred over CBB due to the higher sensitivity obtained. Nevertheless, silver-staining resolution could significantly vary according to the studied organism and more specifically to the researched tissue. In Coffea spp., 2-DE analysis has been rarely employed. Some studies of protein expression have been reported in this culture mainly involving the biosynthesis of caffeine and metabolism during seed germination. The study of the global protein expression in coffee plants in response to biotic stress conditions had not been reported until now. Phytonematode infection can be considered one of the most important biotic stresses that affect coffee production and Meloidogyne paranaensis is one of the major nematode species that infects coffee plants. In this report, the protein expression of infected- and non-infected roots of coffee (Coffea canephora) were analyzed and the protein pattern determined by 2-DE. Gels were stained with silver nitrate or CBB, in order to obtain an optimized method for proteomic analysis of plant-nematode interaction. The 2-DE analysis revealed an enhanced number of protein spots, as well as differentially expressed proteins, when CBB was used. A total of approximately 70 and 100 spots were observed in silver and CBB stained gels, respectively. Moreover, 18 differentially expressed proteins were observed in CBB gels, and only 8 in the silver stained gels. This report showed that the staining method was crucial for an optimized protein analysis of coffee. Similar results were obtained for cotton roots and therefore these results may be extended to other plant species in order to better understand the host-pathogen interaction. MenosProteomic methods, such as bidimensional electrophoresis (2-DE) and mass spectrometry, have been extensively used for the study of protein differential expression in several plants including Arabidopsis thaliana, rice and wheat. Specifically in the 2-DE method, deep attention must be given to the protein staining technique, which often involves silver nitrate or Coomassie Brilliant Blue (CBB). Silver staining is usually preferred over CBB due to the higher sensitivity obtained. Nevertheless, silver-staining resolution could significantly vary according to the studied organism and more specifically to the researched tissue. In Coffea spp., 2-DE analysis has been rarely employed. Some studies of protein expression have been reported in this culture mainly involving the biosynthesis of caffeine and metabolism during seed germination. The study of the global protein expression in coffee plants in response to biotic stress conditions had not been reported until now. Phytonematode infection can be considered one of the most important biotic stresses that affect coffee production and Meloidogyne paranaensis is one of the major nematode species that infects coffee plants. In this report, the protein expression of infected- and non-infected roots of coffee (Coffea canephora) were analyzed and the protein pattern determined by 2-DE. Gels were stained with silver nitrate or CBB, in order to obtain an optimized method for proteomic analysis of plant-nematode interaction. The 2-DE analys... Mostrar Tudo |
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Palavras-Chave: |
Bidimensional electrophoresis; Proteomic method. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32592/1/Comparison-of-Different.pdf
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Marc: |
LEADER 02933nam a2200229 a 4500
001 1885752
005 2011-04-13
008 2007 bl uuuu u00u1 u #d
100 1 $aFRANCO, O. L.
245 $aComparison of different staining methods for coffee proteomic analysis.$h[electronic resource]
260 $aIn:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2007. 1 CD-ROM.$c2007
520 $aProteomic methods, such as bidimensional electrophoresis (2-DE) and mass spectrometry, have been extensively used for the study of protein differential expression in several plants including Arabidopsis thaliana, rice and wheat. Specifically in the 2-DE method, deep attention must be given to the protein staining technique, which often involves silver nitrate or Coomassie Brilliant Blue (CBB). Silver staining is usually preferred over CBB due to the higher sensitivity obtained. Nevertheless, silver-staining resolution could significantly vary according to the studied organism and more specifically to the researched tissue. In Coffea spp., 2-DE analysis has been rarely employed. Some studies of protein expression have been reported in this culture mainly involving the biosynthesis of caffeine and metabolism during seed germination. The study of the global protein expression in coffee plants in response to biotic stress conditions had not been reported until now. Phytonematode infection can be considered one of the most important biotic stresses that affect coffee production and Meloidogyne paranaensis is one of the major nematode species that infects coffee plants. In this report, the protein expression of infected- and non-infected roots of coffee (Coffea canephora) were analyzed and the protein pattern determined by 2-DE. Gels were stained with silver nitrate or CBB, in order to obtain an optimized method for proteomic analysis of plant-nematode interaction. The 2-DE analysis revealed an enhanced number of protein spots, as well as differentially expressed proteins, when CBB was used. A total of approximately 70 and 100 spots were observed in silver and CBB stained gels, respectively. Moreover, 18 differentially expressed proteins were observed in CBB gels, and only 8 in the silver stained gels. This report showed that the staining method was crucial for an optimized protein analysis of coffee. Similar results were obtained for cotton roots and therefore these results may be extended to other plant species in order to better understand the host-pathogen interaction.
653 $aBidimensional electrophoresis
653 $aProteomic method
700 1 $aANDRADE, A. E.
700 1 $aBARROS, E. V. S. A.
700 1 $aSA, M. F. G. de
700 1 $aCARNEIRO, R. M. D. G.
700 1 $aCARNEIRO, R.
700 1 $aEIRA, M. T. S. da
700 1 $aROCHA, T. L.
700 1 $aREIS, A. M. dos
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Registro original: |
Embrapa Café (CNPCa) |
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Registro Completo
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
20/07/2016 |
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Data da última atualização: |
17/07/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
FALAVIGNA, V. S.; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. |
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Afiliação: |
Vítor S. Falavigna, Doutorando PPGBCM/UFRGS, CP 15005, 91501-970, Porto Alegre, RS, Brasil. Bolsista CAPES. vitorfalavigna@gmail.com; Márcia Margis-Pinheiro, Professor PPGBCM/UFRGS, CP 15005, CEP 91501-970, Porto Alegre, RS, Brasil. marcia.margis@ufrgs.br; Giancarlo Pasquali, Professor PPGBCM/UFRGS, CP 15005, CEP 91501-970, Porto Alegre, RS, Brasil. pasquali@cbiot.ufrgs.br; LUIS FERNANDO REVERS, CNPUV. |
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Título: |
Análise funcional e potencial biotecnológico do gene DHN11 de macieira (Malus x domestica Borkh.) |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 14. ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 10., 2016, Bento Gonçalves. Resumos...Bento Gonçalves, RS: Embrapa uva e Vinho, 2016. p. 82. |
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Idioma: |
Português |
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Notas: |
(Embrapa Uva e Vinho. Documentos, 99) |
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Conteúdo: |
A deficiência hídrica é o principal fator limitante à agricultura, causando significativas perdas de produtividade nas mais diversas culturas vegetais. Novas tecnologias agrícolas, tais como a geração de plantas geneticamente modificadas melhor adaptadas a condições adversas de cultivo, são necessárias. Em um estudo anterior do grupo caracterizando a família gênica das desidrinas (DHNs) em macieira (Malus x domestica Borkh.), foi verificado que o gene MdDHN11 possui níveis de expressão cerca de 6 mil vezes maior em sementes em relação a outros tecidos. Sugere-se que o acúmulo nos últimos estágios de desenvolvimento de sementes possua papel protetor na tolerância à dessecação. Além disso, as DHNs são proteínas caracterizadas por seu envolvimento na resposta a mudanças na disponibilidade de água, principalmente os causados por estresses abióticos. |
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Palavras-Chave: |
Anais; CNPUV; Domestica Borkh; Gene DHN11 de macieira; Gênica das desidrinas; IC; Iniciação cientifica; Macieira. |
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Thesagro: |
Deficiência Hídrica; Maca. |
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Thesaurus NAL: |
Malus. |
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Categoria do assunto: |
A Sistemas de Cultivo |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/145664/1/Doc99-falavigna-p82.pdf
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Marc: |
LEADER 01879nam a2200289 a 4500
001 2049323
005 2019-07-17
008 2016 bl uuuu u00u1 u #d
100 1 $aFALAVIGNA, V. S.
245 $aAnálise funcional e potencial biotecnológico do gene DHN11 de macieira (Malus x domestica Borkh.)$h[electronic resource]
260 $aIn: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 14. ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 10., 2016, Bento Gonçalves. Resumos...Bento Gonçalves, RS: Embrapa uva e Vinho, 2016. p. 82.$c2016
500 $a(Embrapa Uva e Vinho. Documentos, 99)
520 $aA deficiência hídrica é o principal fator limitante à agricultura, causando significativas perdas de produtividade nas mais diversas culturas vegetais. Novas tecnologias agrícolas, tais como a geração de plantas geneticamente modificadas melhor adaptadas a condições adversas de cultivo, são necessárias. Em um estudo anterior do grupo caracterizando a família gênica das desidrinas (DHNs) em macieira (Malus x domestica Borkh.), foi verificado que o gene MdDHN11 possui níveis de expressão cerca de 6 mil vezes maior em sementes em relação a outros tecidos. Sugere-se que o acúmulo nos últimos estágios de desenvolvimento de sementes possua papel protetor na tolerância à dessecação. Além disso, as DHNs são proteínas caracterizadas por seu envolvimento na resposta a mudanças na disponibilidade de água, principalmente os causados por estresses abióticos.
650 $aMalus
650 $aDeficiência Hídrica
650 $aMaca
653 $aAnais
653 $aCNPUV
653 $aDomestica Borkh
653 $aGene DHN11 de macieira
653 $aGênica das desidrinas
653 $aIC
653 $aIniciação cientifica
653 $aMacieira
700 1 $aMARGIS-PINHEIRO, M.
700 1 $aPASQUALI, G.
700 1 $aREVERS, L. F.
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Embrapa Uva e Vinho (CNPUV) |
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