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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
01/09/2008 |
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Data da última atualização: |
29/06/2018 |
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Autoria: |
RIBEIRO, L. M.; PEIXOTO, J. R.; ANDRADE, S. R. M. de; FONSECA, R. S.; VIEIRA, L. M.; PEREIRA, W. V. S. |
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Afiliação: |
Leonardo Monteiro Ribeiro, Universidade Estadual de Montes Claros/Departamento de Biologia Geral; José Ricardo Peixoto, Universidade de Brasília/Faculdade de Agronomia e Medicina Veterinária; SOLANGE ROCHA MONTEIRO DE ANDRADE, CPAC; Rúbia Santos Fonseca, Universidade Federal de Viçosa/Departamento de Biologia Vegetal; Lorena Melo Vieira, Universidade Estadual de Montes Claros/Departamento de Biologia Geral; Wilson Vicente Souza Pereira, Universidade Estadual de Montes Claros/Departamento de Biologia Geral. |
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Título: |
Microenxertia ex vitro para eliminação do vírus CABMV em maracujá-azedo. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 5, p. 589-594, maio 2008 |
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Idioma: |
Português |
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Notas: |
Título em inglês: Ex vitro micrografting aiming the CABMV virus elimination in passion fruit plant. |
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Conteúdo: |
O objetivo deste trabalho foi avaliar diferentes meios de cultura, utilizados sobre o ponto da enxertia, na microenxertia ex vitro para a eliminação do Cowpea aphid-borne mosaic virus (CABMV), em plantas de maracujá-azedo (Passiflora edulis Sims f. flavicarpa Deg.). Ápices caulinares, provenientes de plantas infectadas, foram microenxertados em plântulas obtidas pela germinação de sementes em substrato comercial esterilizado. Foram conduzidos experimentos com a microenxertia realizada no hipocótilo e no epicótilo, e foram utilizados cinco meios de cultura, que diferiam na concentração de fitorreguladores, aplicados no local da enxertia. O índice médio de microenxertos com folha expandida foi de 27,22 e 32,22%, quando a microenxertia foi realizada no hipocótilo e no epicótilo, respectivamente. Na microenxertia realizada no hipocótilo, não houve efeito da aplicação de meios de cultura. Na microenxertia realizada no epicótilo, o meio MS acrescido de 0,1 mg L-1 de AIB e 1 mg L-1 de BAP proporcionou 53,3% de microenxertos com folha expandida, número superior aos demais tratamentos e maior desenvolvimento das brotações. A indexação realizada pelo teste ELISA indireto, 80 a 100 dias após a microenxertia, mostrou que 93% das plantas testadas não apresentavam vírus detectável. |
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Palavras-Chave: |
endurecimento dos frutos; grafting spot; local de enxertia; woodiness fruit. |
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Thesagro: |
Passiflora Edulis. |
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Thesaurus Nal: |
Cowpea aphid-borne mosaic virus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/44193/1/43n05a06.pdf
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Marc: |
LEADER 02202naa a2200265 a 4500
001 1125939
005 2018-06-29
008 2008 bl uuuu u00u1 u #d
100 1 $aRIBEIRO, L. M.
245 $aMicroenxertia ex vitro para eliminação do vírus CABMV em maracujá-azedo.
260 $c2008
500 $aTítulo em inglês: Ex vitro micrografting aiming the CABMV virus elimination in passion fruit plant.
520 $aO objetivo deste trabalho foi avaliar diferentes meios de cultura, utilizados sobre o ponto da enxertia, na microenxertia ex vitro para a eliminação do Cowpea aphid-borne mosaic virus (CABMV), em plantas de maracujá-azedo (Passiflora edulis Sims f. flavicarpa Deg.). Ápices caulinares, provenientes de plantas infectadas, foram microenxertados em plântulas obtidas pela germinação de sementes em substrato comercial esterilizado. Foram conduzidos experimentos com a microenxertia realizada no hipocótilo e no epicótilo, e foram utilizados cinco meios de cultura, que diferiam na concentração de fitorreguladores, aplicados no local da enxertia. O índice médio de microenxertos com folha expandida foi de 27,22 e 32,22%, quando a microenxertia foi realizada no hipocótilo e no epicótilo, respectivamente. Na microenxertia realizada no hipocótilo, não houve efeito da aplicação de meios de cultura. Na microenxertia realizada no epicótilo, o meio MS acrescido de 0,1 mg L-1 de AIB e 1 mg L-1 de BAP proporcionou 53,3% de microenxertos com folha expandida, número superior aos demais tratamentos e maior desenvolvimento das brotações. A indexação realizada pelo teste ELISA indireto, 80 a 100 dias após a microenxertia, mostrou que 93% das plantas testadas não apresentavam vírus detectável.
650 $aCowpea aphid-borne mosaic virus
650 $aPassiflora Edulis
653 $aendurecimento dos frutos
653 $agrafting spot
653 $alocal de enxertia
653 $awoodiness fruit
700 1 $aPEIXOTO, J. R.
700 1 $aANDRADE, S. R. M. de
700 1 $aFONSECA, R. S.
700 1 $aVIEIRA, L. M.
700 1 $aPEREIRA, W. V. S.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 43, n. 5, p. 589-594, maio 2008
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Embrapa Unidades Centrais (AI-SEDE) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
13/02/2017 |
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Data da última atualização: |
30/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
WELLER, M. M.; ALBINO, R. L.; MARCONDES, M. I.; SILVA, W.; DANIELS, K. M.; CAMPOS, M. M.; DUARTE, M. S.; MESCOUTO, M. L.; SILVA, F. F.; GUIMARÃES, S. E. F. |
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Afiliação: |
M. M. WELLER, UFV; R. L. ALBINO, UFV; M. I. MARCONDES, UFV; W. SILVA, UFV; K. M. DANIELS, Virginia Polytechnic Institute and State University, Blacksburg; MARIANA MAGALHAES CAMPOS, CNPGL; M. S. DUARTE, UFV; M. L. MESCOUTO, UFV; F. F. SILVA, UFV; S. E. F. GUIMARÃES, UFV. |
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Título: |
Effects of nutrient intake level on mammary parenchyma growth and gene expression in crossbred (Holstein × Gyr) prepubertal heifers. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Journal of Dairy Science, v. 99, n. 12, p. 9962-9973, 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract This study investigated the effects of increased nutrient intake levels on prepubertal mammary parenchyma development in crossbreed (Holstein × Gyr) dairy heifers. Eighteen heifers age 3 to 4 mo were fed 1 of 3 nutrient intake levels (n=6 per treatment) designed to sustain an average daily gain of 0.0kg/d (maintenance, MA), 0.5kg/d (low gain, LG), or 1.0kg/d (high gain, HG). Serum blood samples collected on d 42 and 84 after a 12-h fast were analyzed for triglycerides, leptin, insulin, and insulin-like growth factor 1 (IGF-1). Liver and mammary parenchyma were biopsied on d 42 and harvested on d 84 for gene expression analysis. Parenchyma samples were also used for biochemical and histological analysis. Mammary parenchyma weight was lower in HG than in MA or LG heifers, but mammary extraparenchymal fat was greater in HG heifers than in other groups. Heifers fed the HG diet had a greater fraction of ether extract in their parenchyma than the others and a smaller fraction of crude protein in their parenchyma than MA heifers. Moreover, the HG and LG heifers had greater body fat mass than MA heifers. Nutrient intake level had no effect on the number of intraparenchymal adipocytes. Heifers fed the HG diet had greater serum IGF-1 than the others, and serum insulin was lower in the MA than the HG or LG heifers. Liver GHR, IGF1, and IGFBP3 mRNA expression was higher, but IGFBP2 mRNA was lower in HG heifers than in others. The parenchyma mRNA expression of lipogenic markers, such as CD36, ACCA, FASN, and ADIPOR1, was upregulated by nutrient intake level. Significant nutrient intake × time interactions for lipogenic genes during the experimental period indicated variable gene expression depending on the time point of prepubertal mammary gland development. Overall, our data suggest that enhancing nutrient intake increased body fat accumulation and lipogenesis in the mammary gland to the detriment of parenchyma growth. Moreover, increased lipogenesis in the parenchyma of HG heifers may indicate that fat accumulation occurred because of adipocyte hypertrophy and not differences in adipogenesis. The implications of these results for milk yield needs to be elucidated. MenosAbstract This study investigated the effects of increased nutrient intake levels on prepubertal mammary parenchyma development in crossbreed (Holstein × Gyr) dairy heifers. Eighteen heifers age 3 to 4 mo were fed 1 of 3 nutrient intake levels (n=6 per treatment) designed to sustain an average daily gain of 0.0kg/d (maintenance, MA), 0.5kg/d (low gain, LG), or 1.0kg/d (high gain, HG). Serum blood samples collected on d 42 and 84 after a 12-h fast were analyzed for triglycerides, leptin, insulin, and insulin-like growth factor 1 (IGF-1). Liver and mammary parenchyma were biopsied on d 42 and harvested on d 84 for gene expression analysis. Parenchyma samples were also used for biochemical and histological analysis. Mammary parenchyma weight was lower in HG than in MA or LG heifers, but mammary extraparenchymal fat was greater in HG heifers than in other groups. Heifers fed the HG diet had a greater fraction of ether extract in their parenchyma than the others and a smaller fraction of crude protein in their parenchyma than MA heifers. Moreover, the HG and LG heifers had greater body fat mass than MA heifers. Nutrient intake level had no effect on the number of intraparenchymal adipocytes. Heifers fed the HG diet had greater serum IGF-1 than the others, and serum insulin was lower in the MA than the HG or LG heifers. Liver GHR, IGF1, and IGFBP3 mRNA expression was higher, but IGFBP2 mRNA was lower in HG heifers than in others. The parenchyma mRNA expression of lipogenic markers,... Mostrar Tudo |
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Palavras-Chave: |
Dairy heifer; Mammary lipogenesis. |
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Thesaurus NAL: |
nutrigenomics. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03014naa a2200265 a 4500
001 2063812
005 2023-01-30
008 2016 bl uuuu u00u1 u #d
100 1 $aWELLER, M. M.
245 $aEffects of nutrient intake level on mammary parenchyma growth and gene expression in crossbred (Holstein × Gyr) prepubertal heifers.$h[electronic resource]
260 $c2016
520 $aAbstract This study investigated the effects of increased nutrient intake levels on prepubertal mammary parenchyma development in crossbreed (Holstein × Gyr) dairy heifers. Eighteen heifers age 3 to 4 mo were fed 1 of 3 nutrient intake levels (n=6 per treatment) designed to sustain an average daily gain of 0.0kg/d (maintenance, MA), 0.5kg/d (low gain, LG), or 1.0kg/d (high gain, HG). Serum blood samples collected on d 42 and 84 after a 12-h fast were analyzed for triglycerides, leptin, insulin, and insulin-like growth factor 1 (IGF-1). Liver and mammary parenchyma were biopsied on d 42 and harvested on d 84 for gene expression analysis. Parenchyma samples were also used for biochemical and histological analysis. Mammary parenchyma weight was lower in HG than in MA or LG heifers, but mammary extraparenchymal fat was greater in HG heifers than in other groups. Heifers fed the HG diet had a greater fraction of ether extract in their parenchyma than the others and a smaller fraction of crude protein in their parenchyma than MA heifers. Moreover, the HG and LG heifers had greater body fat mass than MA heifers. Nutrient intake level had no effect on the number of intraparenchymal adipocytes. Heifers fed the HG diet had greater serum IGF-1 than the others, and serum insulin was lower in the MA than the HG or LG heifers. Liver GHR, IGF1, and IGFBP3 mRNA expression was higher, but IGFBP2 mRNA was lower in HG heifers than in others. The parenchyma mRNA expression of lipogenic markers, such as CD36, ACCA, FASN, and ADIPOR1, was upregulated by nutrient intake level. Significant nutrient intake × time interactions for lipogenic genes during the experimental period indicated variable gene expression depending on the time point of prepubertal mammary gland development. Overall, our data suggest that enhancing nutrient intake increased body fat accumulation and lipogenesis in the mammary gland to the detriment of parenchyma growth. Moreover, increased lipogenesis in the parenchyma of HG heifers may indicate that fat accumulation occurred because of adipocyte hypertrophy and not differences in adipogenesis. The implications of these results for milk yield needs to be elucidated.
650 $anutrigenomics
653 $aDairy heifer
653 $aMammary lipogenesis
700 1 $aALBINO, R. L.
700 1 $aMARCONDES, M. I.
700 1 $aSILVA, W.
700 1 $aDANIELS, K. M.
700 1 $aCAMPOS, M. M.
700 1 $aDUARTE, M. S.
700 1 $aMESCOUTO, M. L.
700 1 $aSILVA, F. F.
700 1 $aGUIMARÃES, S. E. F.
773 $tJournal of Dairy Science$gv. 99, n. 12, p. 9962-9973, 2016.
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