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Registros recuperados : 9 | |
1. | | ESTEVAM, D. de O.; MARCOLINO, J.; LANZARINI, J. J. S.; MADEIRA, K. Cooperativismo virtual: o caso da cooperativa de produção agroindustrial familiar de Nova Veneza (COOFANOVE), em Santa Catarina. Cadernos de Ciência & Tecnologia, Brasília, DF, v. 28, n. 2, p. 485-507, maio./ago. 2011. Biblioteca(s): Embrapa Unidades Centrais. |
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2. | | NAKAYAMA, T. J.; RODRIGUES, F. A.; MARCOLINO, J.; MARCELINO, F. C.; SILVA, F. R. da; OLIVEIRA, A. C. B. de; EMYGDIO, B. M.; NEPOMUCENO, A. L.; NEUMAIER, N. Transcriptome analysis of soybean (Glycine max L. Merrill) root submited to hypoxic condition. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. p. 154-155. Biblioteca(s): Embrapa Soja. |
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3. | | RODRIGUES, F. A.; MARCOLINO, J.; ENGELS, C.; NAKAYAMA, T. J.; FUGANTI, R.; MARIN, S. R.; FARIAS, J. R. B.; NEUMAIER, N.; MARCELINO, F. C.; BINNECK, E.; ABDELNOOR, R. V.; NEPOMUCENO, A. L. Prospecting soybean (Glycine max L. Merrill) genes expressed under drought conditions. In: CONGRESSO BRASILEIRO DE GENÉTICA, 55., 2009, Águas de Lindóia. Charles Darwin: a origem das espécies: o livro que transformou a humanidade. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2009. p. 92. Biblioteca(s): Embrapa Soja. |
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4. | | RODRIGUES, F. A.; MARCOLINO, J.; NAKAYAMA, T. J.; FUGANTI, R.; ENGELS, C.; MARIN, S. R. R.; FARIAS, J. R. B.; NEUMAIER, N.; MARCELINO, F. C.; BINNECK, E.; ABDELNOOR, R. V.; NEPOMUCENO, A. L. Prospecting soybean (Glycine Max L. Merrill) genes expressed under drought conditions. In: INTERNATIONAL CONFERENCE ON INTEGRATED APPROACHES TO IMPROVE CROP PRODUCTION UNDER DROUGHT-PRONE ENVIRONMENTS, 3., 2009, Shangai. Abstracts... Shangai: Shangai Academy of Agricultural Sciences: Shangai Agrobiological Gene Center, 2009. p. 178, ref. P 5.35. INTERDROUGHT 2009. Biblioteca(s): Embrapa Soja. |
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5. | | AMARAL, L. C.; RODRIGUES, F. A.; MARCOLINO, J.; NASCIMENTO, L. C.; PEREIRA, R. M.; CARAZZOLLE, M. F.; OLIVEIRA, M. C. N. de; MARCELINO, F. C.; ABDELNOOR, R. V.; NEUMAIER, N.; FARIAS, J. R. B.; NEPOMUCENO, A. L. Genes related to cell apparatus protection and osmotic adjustment expressed in drought-stressed soybean plants. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. p. 257-258. Biblioteca(s): Embrapa Soja. |
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6. | | MARCOLINO, J.; RODRIGUES, F. A.; AMARAL, L. C.; NAKAYAMA, T. J.; PEREIRA, R. M.; NASCIMENTO, L. C. do; BINNECK, E.; OLIVEIRA, M. C. N. de; MARCELINO, F. C.; ABDELNOOR, R. V.; NEUMAIER, N.; FARIAS, J. R. B.; NEPOMUCENO, A. L. Transcription factors expressed in soybean roots in response to water déficit. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. p. 193-194. Biblioteca(s): Embrapa Soja. |
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7. | | RODRIGUES, F. A.; MARCOLINO, J.; AMARAL, L. C.; ENGELS, C.; PEREIRA, R. M.; CARAZZOLLE, M. F.; OLIVEIRA, M. C. N. de; MARCELINO, F. C.; FARIAS, J. R. B.; NEUMAIER, N.; BINNECK, E.; ABDELNOOR, R. V.; NEPOMUCENO, A. L. Comparison of gene expression profiles of contrasting soybean cultivars in response to drought. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. p. 189. Biblioteca(s): Embrapa Soja. |
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8. | | KULCHESKI, F. R.; OLIVEIRA, L. F. V.; MOLINA, L. G.; ALMERAO, M. P.; RODRIGUES, F. A.; MARCOLINO, J.; BARBOSA, J. F.; STOLF-MOREIRA, R.; NEPOMUCENO, A. L.; MARCELINO-GUIMARÃES, F. C.; ABDELNOOR, R. V.; NASCIMENTO, L. C.; CARAZZOLLE, M. F.; PEREIRA, G. A. G.; MARGIS, R. Identification of novel soybean microRNAs involved in abiotic and biotic stresses. BMC Genomics, v. 12, n. 307, jun. 2011. 17 p. Biblioteca(s): Embrapa Soja. |
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9. | | KULCHESKI, F. R.; OLIVEIRA, L. F. V.; MOLINA, L. G.; ALMERÃO, M.; RODRIGUES, F. A.; MARCOLINO, J.; BARBOSA, J. F.; STOLF-MOREIRA, R.; NEPOMUCENO, A. L.; MARCELINO, F. C.; ABDELNOOR, R. V.; NASCIMENTO, L. C.; CARAZZOLLE, M. F.; PEREIRA, G. A. G.; MARGIS, R. Identification of novel soybean microRNAs involved in abiotic and biotic stresses. In: BRAZILIAN SYMPOSIUM ON PLANT MOLECULAR BIOLOGY, 3., 2011, Ilhéus. [Abstracts...]. [Ribeirão Preto]: SBG, 2011. 1 p. 1 CD-ROM. Biblioteca(s): Embrapa Soja. |
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Registros recuperados : 9 | |
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Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
05/01/2012 |
Data da última atualização: |
03/04/2013 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
KULCHESKI, F. R.; OLIVEIRA, L. F. V.; MOLINA, L. G.; ALMERAO, M. P.; RODRIGUES, F. A.; MARCOLINO, J.; BARBOSA, J. F.; STOLF-MOREIRA, R.; NEPOMUCENO, A. L.; MARCELINO-GUIMARÃES, F. C.; ABDELNOOR, R. V.; NASCIMENTO, L. C.; CARAZZOLLE, M. F.; PEREIRA, G. A. G.; MARGIS, R. |
Afiliação: |
FRANCELI R. KULCHESKI, UFRGS; LUIZ F. V. DE OLIVEIRA, UFRGS; LORRAYNE, G. MOLINA, UFRGS; MAURÍCIO P. ALMERÃO, UFRGS; FABIANA A. RODRIGUES, CNPSo; JULIANA MARCOLINO, CNPSo; JOICE F. BARBOSA, CNPSo; RENATA STOLF MOREIRA, CNPSo; ALEXANDRE LIMA NEPOMUCENO, SRI; FRANCISMAR CORREA MARCELINO GUIMARÃES, CNPSO; RICARDO VILELA ABDELNOOR, CNPSO; LEANDRO C. NASCIMENTO, UNICAMP; MARCELO F. CARAZZOLLE, UNICAMP; GONÇALO A. G. PEREIRA, UNICAMP; ROGÉRIO MARGIS, UFRGS. |
Título: |
Identification of novel soybean microRNAs involved in abiotic and biotic stresses. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
BMC Genomics, v. 12, n. 307, jun. 2011. |
Páginas: |
17 p. |
DOI: |
10.1186/1471-2164-12-307 |
Idioma: |
Português |
Conteúdo: |
Background: Small RNAs (19-24 nt) are key regulators of gene expression that guide both transcriptional and posttranscriptional silencing mechanisms in eukaryotes. Current studies have demonstrated that microRNAs (miRNAs) act in several plant pathways associated with tissue proliferation, differentiation, and development and in response to abiotic and biotic stresses. In order to identify new miRNAs in soybean and to verify those that are possibly water deficit and rust-stress regulated, eight libraries of small RNAs were constructed and submitted to Solexa sequencing. Results: The libraries were developed from drought-sensitive and tolerant seedlings and rust-susceptible and resistant soybeans with or without stressors. Sequencing the library and subsequent analyses detected 256 miRNAs. From this total, we identified 24 families of novel miRNAs that had not been reported before, six families of conserved miRNAs that exist in other plants species, and 22 families previously reported in soybean. We also observed the presence of several isomiRNAs during our analyses. To validate novel miRNAs, we performed RT-qPCR across the eight different libraries. Among the 11 miRNAs analyzed, all showed different expression profiles during biotic and abiotic stresses to soybean. The majority of miRNAs were up-regulated during water deficit stress in the sensitive plants. However, for the tolerant genotype, most of the miRNAs were down regulated. The pattern of miRNAs expression was also different for the distinct genotypes submitted to the pathogen stress. Most miRNAs were down regulated during the fungus infection in the susceptible genotype; however, in the resistant genotype, most miRNAs did not vary during rust attack. A prediction of the putative targets was carried out for conserved and novel miRNAs families. Conclusions: Validation of our results with quantitative RT-qPCR revealed that Solexa sequencing is a powerful tool for miRNA discovery. The identification of differentially expressed plant miRNAs provides molecular evidence for the possible involvement of miRNAs in the process of water deficit- and rust-stress responses. MenosBackground: Small RNAs (19-24 nt) are key regulators of gene expression that guide both transcriptional and posttranscriptional silencing mechanisms in eukaryotes. Current studies have demonstrated that microRNAs (miRNAs) act in several plant pathways associated with tissue proliferation, differentiation, and development and in response to abiotic and biotic stresses. In order to identify new miRNAs in soybean and to verify those that are possibly water deficit and rust-stress regulated, eight libraries of small RNAs were constructed and submitted to Solexa sequencing. Results: The libraries were developed from drought-sensitive and tolerant seedlings and rust-susceptible and resistant soybeans with or without stressors. Sequencing the library and subsequent analyses detected 256 miRNAs. From this total, we identified 24 families of novel miRNAs that had not been reported before, six families of conserved miRNAs that exist in other plants species, and 22 families previously reported in soybean. We also observed the presence of several isomiRNAs during our analyses. To validate novel miRNAs, we performed RT-qPCR across the eight different libraries. Among the 11 miRNAs analyzed, all showed different expression profiles during biotic and abiotic stresses to soybean. The majority of miRNAs were up-regulated during water deficit stress in the sensitive plants. However, for the tolerant genotype, most of the miRNAs were down regulated. The pattern of miRNAs expression was also di... Mostrar Tudo |
Thesagro: |
Deficiência hídrica; Gene marcador; Soja. |
Thesaurus NAL: |
Gene expression; Soil water deficit; Soybeans. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/51756/1/BMC-2011.pdf
|
Marc: |
LEADER 03216naa a2200385 a 4500 001 1911802 005 2013-04-03 008 2011 bl uuuu u00u1 u #d 024 7 $a10.1186/1471-2164-12-307$2DOI 100 1 $aKULCHESKI, F. R. 245 $aIdentification of novel soybean microRNAs involved in abiotic and biotic stresses. 260 $c2011 300 $a17 p. 520 $aBackground: Small RNAs (19-24 nt) are key regulators of gene expression that guide both transcriptional and posttranscriptional silencing mechanisms in eukaryotes. Current studies have demonstrated that microRNAs (miRNAs) act in several plant pathways associated with tissue proliferation, differentiation, and development and in response to abiotic and biotic stresses. In order to identify new miRNAs in soybean and to verify those that are possibly water deficit and rust-stress regulated, eight libraries of small RNAs were constructed and submitted to Solexa sequencing. Results: The libraries were developed from drought-sensitive and tolerant seedlings and rust-susceptible and resistant soybeans with or without stressors. Sequencing the library and subsequent analyses detected 256 miRNAs. From this total, we identified 24 families of novel miRNAs that had not been reported before, six families of conserved miRNAs that exist in other plants species, and 22 families previously reported in soybean. We also observed the presence of several isomiRNAs during our analyses. To validate novel miRNAs, we performed RT-qPCR across the eight different libraries. Among the 11 miRNAs analyzed, all showed different expression profiles during biotic and abiotic stresses to soybean. The majority of miRNAs were up-regulated during water deficit stress in the sensitive plants. However, for the tolerant genotype, most of the miRNAs were down regulated. The pattern of miRNAs expression was also different for the distinct genotypes submitted to the pathogen stress. Most miRNAs were down regulated during the fungus infection in the susceptible genotype; however, in the resistant genotype, most miRNAs did not vary during rust attack. A prediction of the putative targets was carried out for conserved and novel miRNAs families. Conclusions: Validation of our results with quantitative RT-qPCR revealed that Solexa sequencing is a powerful tool for miRNA discovery. The identification of differentially expressed plant miRNAs provides molecular evidence for the possible involvement of miRNAs in the process of water deficit- and rust-stress responses. 650 $aGene expression 650 $aSoil water deficit 650 $aSoybeans 650 $aDeficiência hídrica 650 $aGene marcador 650 $aSoja 700 1 $aOLIVEIRA, L. F. V. 700 1 $aMOLINA, L. G. 700 1 $aALMERAO, M. P. 700 1 $aRODRIGUES, F. A. 700 1 $aMARCOLINO, J. 700 1 $aBARBOSA, J. F. 700 1 $aSTOLF-MOREIRA, R. 700 1 $aNEPOMUCENO, A. L. 700 1 $aMARCELINO-GUIMARÃES, F. C. 700 1 $aABDELNOOR, R. V. 700 1 $aNASCIMENTO, L. C. 700 1 $aCARAZZOLLE, M. F. 700 1 $aPEREIRA, G. A. G. 700 1 $aMARGIS, R. 773 $tBMC Genomics$gv. 12, n. 307, jun. 2011.
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