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Registro Completo |
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Biblioteca(s): |
Embrapa Hortaliças; Embrapa Unidades Centrais. |
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Data corrente: |
24/05/1995 |
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Data da última atualização: |
30/08/2019 |
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Autoria: |
SILVA, A. T. da; PASQUAL, M.; ISHIDA, J. S.; ANTUNES, L. E. C. |
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Afiliação: |
Antonio Tadeu da Silva, Escola Superior de Agricultura de Lavras - ESAL/Departamento de Agricultura; Moacir Pasqual, Escola Superior de Agricultura de Lavras - ESAL. Bolsista do CNPq; Jorge Susumu Ishida, Escola Superior de Agricultura de Lavras - ESAL. Bolsista do CNPq; Luís Eduardo Corrêa Antunes, Escola Superior de Agricultura de Lavras - ESAL. Bolsista do CNPq. |
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Título: |
Aclimatação de plantas provenientes da cultura in vitro. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 30, n. 1, p. 49-53, jan. 1995. |
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Idioma: |
Português |
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Notas: |
Título em inglês: Acclimatization of plants produced by in vitro culture. |
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Conteúdo: |
Objetivou-se estudar o efeito das idades das raízes emergidas in vitro, substratos e espécie na capacidade de aclimatação após sair da cultura de tecido para casa de vegetação. |
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Palavras-Chave: |
Acclimatization; Culture; In Vitro; Krelmeyera coreacea; Sweet potato; Tissue. |
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Thesagro: |
Amora Preta; Batata Doce; Cultura de Tecido; Enraizamento; Ipomoea Batatas; Mandioca; Manihot Esculenta; Pau Santo; Rubus Idaeus. |
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Thesaurus Nal: |
cassava; rooting. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/201437/1/Aclimatacao-de-plantas-provenientes.pdf
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Marc: |
LEADER 01216naa a2200373 a 4500
001 2111747
005 2019-08-30
008 1995 bl uuuu u00u1 u #d
100 1 $aSILVA, A. T. da
245 $aAclimatação de plantas provenientes da cultura in vitro.
260 $c1995
500 $aTítulo em inglês: Acclimatization of plants produced by in vitro culture.
520 $aObjetivou-se estudar o efeito das idades das raízes emergidas in vitro, substratos e espécie na capacidade de aclimatação após sair da cultura de tecido para casa de vegetação.
650 $acassava
650 $arooting
650 $aAmora Preta
650 $aBatata Doce
650 $aCultura de Tecido
650 $aEnraizamento
650 $aIpomoea Batatas
650 $aMandioca
650 $aManihot Esculenta
650 $aPau Santo
650 $aRubus Idaeus
653 $aAcclimatization
653 $aCulture
653 $aIn Vitro
653 $aKrelmeyera coreacea
653 $aSweet potato
653 $aTissue
700 1 $aPASQUAL, M.
700 1 $aISHIDA, J. S.
700 1 $aANTUNES, L. E. C.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 30, n. 1, p. 49-53, jan. 1995.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
11/07/2013 |
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Data da última atualização: |
02/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
OLIVEIRA, L. R.; RODRIGUES, E. P.; MARCELINO-GUIMARÃES, F. C.; OLIVEIRA, A. L. M.; HUNGRIA, M. |
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Afiliação: |
LUCIANA RUANO OLIVEIRA, UEL; ELISETE PAINS RODRIGUES, UEL; FRANCISMAR CORREA MARCELINO-GUIMARÃES, CNPSO; ANDRÉ LUIZ MARTINEZ OLIVEIRA, UEL; MARIANGELA HUNGRIA, CNPSO. |
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Título: |
Fast induction of biosynthetic polysaccharide genes lpxA, lpxE, and rkpI of Rhizobium sp. strain PRF 81 by common bean seed exudates is indicative of a key role in symbiosis. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Functional & Integrative Genomics, v. 13, n. 2, p. 275-283, Jun. 2013. |
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ISSN: |
1438-7948 |
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DOI: |
10.1007/s10142-013-0322-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Rhizobial surface polysaccharides (SPS) are, together with nodulation (Nod) factors, recognized as key molecules for establishment of rhizobia?legume symbiosis. In Rhizobium tropici, an important nitrogen-fixing symbiont of common bean (Phaseolus vulgaris L.), molecular structures and symbiotic roles of the SPS are poorly understood. In this study, Rhizobium sp. strain PRF 81 genes, belonging to the R. tropici group, were investigated: lpxA and lpxE, involved in biosynthesis and modification of the lipid-A anchor of lipopolysaccharide (LPS), and rkpI, involved in synthesis of a lipid carrier required for production of capsular polysaccharides (KPS). Reverse transcription quantitative PCR (RT-qPCR) analysis revealed, for the first time, that inducers released from common bean seeds strongly stimulated expression of all three SPS genes. When PRF 81 cells were grown for 48 h in the presence of seed exudates, twofold increases (p < 0.05) in the transcription levels of lpxE, lpxA, and rkpI genes were observed. However, higher increases (p < 0.05) in transcription rates, about 50-fold for lpxE and about 30-fold for lpxA and rkpI, were observed after only 5 min of incubation with common bean seed exudates. Evolutionary analyses revealed that lpxA and lpxE of PRF81 and of the type strain of R. tropici CIAT899Tclustered with orthologous Rhizobium radiobacter and were more related to R. etli and Rhizobium leguminosarum, while rkpI was closer to the Sinorhizobium sp. group. Upregulation of lpxE, lpxA, and rkpI genes suggests that seed exudates can modulate production of SPS of Rhizobium sp. PRF81, leading to cell wall changes necessary for symbiosis establishment. MenosAbstract: Rhizobial surface polysaccharides (SPS) are, together with nodulation (Nod) factors, recognized as key molecules for establishment of rhizobia?legume symbiosis. In Rhizobium tropici, an important nitrogen-fixing symbiont of common bean (Phaseolus vulgaris L.), molecular structures and symbiotic roles of the SPS are poorly understood. In this study, Rhizobium sp. strain PRF 81 genes, belonging to the R. tropici group, were investigated: lpxA and lpxE, involved in biosynthesis and modification of the lipid-A anchor of lipopolysaccharide (LPS), and rkpI, involved in synthesis of a lipid carrier required for production of capsular polysaccharides (KPS). Reverse transcription quantitative PCR (RT-qPCR) analysis revealed, for the first time, that inducers released from common bean seeds strongly stimulated expression of all three SPS genes. When PRF 81 cells were grown for 48 h in the presence of seed exudates, twofold increases (p < 0.05) in the transcription levels of lpxE, lpxA, and rkpI genes were observed. However, higher increases (p < 0.05) in transcription rates, about 50-fold for lpxE and about 30-fold for lpxA and rkpI, were observed after only 5 min of incubation with common bean seed exudates. Evolutionary analyses revealed that lpxA and lpxE of PRF81 and of the type strain of R. tropici CIAT899Tclustered with orthologous Rhizobium radiobacter and were more related to R. etli and Rhizobium leguminosarum, while rkpI was closer to the Si... Mostrar Tudo |
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Thesagro: |
Feijão. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02449naa a2200205 a 4500
001 1961873
005 2017-08-02
008 2013 bl uuuu u00u1 u #d
022 $a1438-7948
024 7 $a10.1007/s10142-013-0322-7$2DOI
100 1 $aOLIVEIRA, L. R.
245 $aFast induction of biosynthetic polysaccharide genes lpxA, lpxE, and rkpI of Rhizobium sp. strain PRF 81 by common bean seed exudates is indicative of a key role in symbiosis.$h[electronic resource]
260 $c2013
520 $aAbstract: Rhizobial surface polysaccharides (SPS) are, together with nodulation (Nod) factors, recognized as key molecules for establishment of rhizobia?legume symbiosis. In Rhizobium tropici, an important nitrogen-fixing symbiont of common bean (Phaseolus vulgaris L.), molecular structures and symbiotic roles of the SPS are poorly understood. In this study, Rhizobium sp. strain PRF 81 genes, belonging to the R. tropici group, were investigated: lpxA and lpxE, involved in biosynthesis and modification of the lipid-A anchor of lipopolysaccharide (LPS), and rkpI, involved in synthesis of a lipid carrier required for production of capsular polysaccharides (KPS). Reverse transcription quantitative PCR (RT-qPCR) analysis revealed, for the first time, that inducers released from common bean seeds strongly stimulated expression of all three SPS genes. When PRF 81 cells were grown for 48 h in the presence of seed exudates, twofold increases (p < 0.05) in the transcription levels of lpxE, lpxA, and rkpI genes were observed. However, higher increases (p < 0.05) in transcription rates, about 50-fold for lpxE and about 30-fold for lpxA and rkpI, were observed after only 5 min of incubation with common bean seed exudates. Evolutionary analyses revealed that lpxA and lpxE of PRF81 and of the type strain of R. tropici CIAT899Tclustered with orthologous Rhizobium radiobacter and were more related to R. etli and Rhizobium leguminosarum, while rkpI was closer to the Sinorhizobium sp. group. Upregulation of lpxE, lpxA, and rkpI genes suggests that seed exudates can modulate production of SPS of Rhizobium sp. PRF81, leading to cell wall changes necessary for symbiosis establishment.
650 $aFeijão
700 1 $aRODRIGUES, E. P.
700 1 $aMARCELINO-GUIMARÃES, F. C.
700 1 $aOLIVEIRA, A. L. M.
700 1 $aHUNGRIA, M.
773 $tFunctional & Integrative Genomics$gv. 13, n. 2, p. 275-283, Jun. 2013.
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