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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
27/07/1998 |
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Data da última atualização: |
24/08/2012 |
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Autoria: |
KATO, A. K.; UCHIDA, M.; MENEZES, A. J. E. A. de; OGATA, T.; ALBUQUERQUE, F. C. de; HAMADA, M.; DUARTE, M. de L. R. |
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Afiliação: |
ARMANDO KOUZO KATO, CPATU; MAKOTO UCHIDA, CONVÊNIO CPATU/JICA; ANTONIO JOSE ELIAS A DE MENEZES, CPATU; TOSHIO OGATA, CONVÊNIO CPATU/JICA; FERNANDO CARNEIRO DE ALBUQUERQUE, CPATU; MASAHIRO HAMADA, CONVÊNIO CPATU/JICA; MARIA DE LURDES REIS DUARTE, CPATU. |
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Título: |
Utilização de tutores vivos na cultura da pimenta-do-reino. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
In: SEMINÁRIO INTERNACIONAL SOBRE PIMENTA-DO-REINO E CUPUAÇU, 1996, Belém, PA. Anais... Belém, PA: EMBRAPA-CPATU: JICA, 1997. |
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Páginas: |
p. 435-440. |
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Série: |
(EMBRAPA-CPATU. Documentos, 89). |
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Idioma: |
Português |
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Palavras-Chave: |
Cultural methods; Pimenta-do-reino; Staking. |
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Thesagro: |
Azadirachta Indica; Nim; Piper Nigrum; Pratica Cultural; Tutoramento. |
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Thesaurus Nal: |
Gliricidia sepium. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/64931/1/Doc89-p435.pdf
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Marc: |
LEADER 00913nam a2200301 a 4500
001 1394597
005 2012-08-24
008 1997 bl uuuu u00u1 u #d
100 1 $aKATO, A. K.
245 $aUtilização de tutores vivos na cultura da pimenta-do-reino.
260 $aIn: SEMINÁRIO INTERNACIONAL SOBRE PIMENTA-DO-REINO E CUPUAÇU, 1996, Belém, PA. Anais... Belém, PA: EMBRAPA-CPATU: JICA$c1997
300 $ap. 435-440.
490 $a(EMBRAPA-CPATU. Documentos, 89).
650 $aGliricidia sepium
650 $aAzadirachta Indica
650 $aNim
650 $aPiper Nigrum
650 $aPratica Cultural
650 $aTutoramento
653 $aCultural methods
653 $aPimenta-do-reino
653 $aStaking
700 1 $aUCHIDA, M.
700 1 $aMENEZES, A. J. E. A. de
700 1 $aOGATA, T.
700 1 $aALBUQUERQUE, F. C. de
700 1 $aHAMADA, M.
700 1 $aDUARTE, M. de L. R.
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
17/12/2015 |
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Data da última atualização: |
05/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
KUMA, K. M.; LOPES-CAITAR, V. S.; ROMERO, C. C. T.; SILVA, S. M. H.; CARVALHO, M. C. C. G.; ABDELNOOR, R. V.; DIAS, W. P.; MARCELINO-GUIMARÃES, F. C. |
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Afiliação: |
UEL; UEL; CNPSo; UEL; UENP; RICARDO VILELA ABDELNOOR, CNPSO; WALDIR PEREIRA DIAS, CNPSO; FRANCISMAR CORREA MARCELINO GUIMARÃES, CNPSO. |
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Título: |
A high efficient protocol for soybean root transformation by Agrobacterium rhizogenes and most stable reference genes for RT-qPCR analysis. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plant Cell Reports, v. 34, n. 11, p. 1987-2000, 2015. |
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ISSN: |
1432-203X |
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DOI: |
10.1007/s00299-015-1845-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Gene functional analyses are essential to the validation of results obtained from in silico and/or geneprospecting studies. Genetic transformation methods that yield tissues of transient expression quickly have been of considerable interest to researchers. Agrobacterium rhizogenes-mediated transformation methods, which are employed to generate plants with transformed roots, have proven useful for the study of stress caused by root phytopathogens via gene overexpression and/or silencing. While some protocols have been adapted to soybean plants, transformation efficiencies remain limited; thus, few viable plants are available for performing bioassays. Furthermore, mRNA analyses that employ reverse transcription quantitative polymerase chain reactions (RT-qPCR) require the use of reference genes with stable expression levels across different organs, development steps and treatments. In the present study, an A. rhizogenes-mediated soybean root transformation approach was optimized. The method delivers significantly higher transformation efficiency levels and rates of transformed plant recovery, thus enhancing studies of soybean abiotic conditions or interactions between phytopathogens, such as nematodes. A 55 % transformation efficiency was obtained following the addition of an acclimation step that involves hydroponics and different selection processes. The present study also validated the reference genes GmELF1-b and GmELF1-a as the most stable to be used in RT-qPCR analysis in composite plants, mainly under nematode infection. MenosGene functional analyses are essential to the validation of results obtained from in silico and/or geneprospecting studies. Genetic transformation methods that yield tissues of transient expression quickly have been of considerable interest to researchers. Agrobacterium rhizogenes-mediated transformation methods, which are employed to generate plants with transformed roots, have proven useful for the study of stress caused by root phytopathogens via gene overexpression and/or silencing. While some protocols have been adapted to soybean plants, transformation efficiencies remain limited; thus, few viable plants are available for performing bioassays. Furthermore, mRNA analyses that employ reverse transcription quantitative polymerase chain reactions (RT-qPCR) require the use of reference genes with stable expression levels across different organs, development steps and treatments. In the present study, an A. rhizogenes-mediated soybean root transformation approach was optimized. The method delivers significantly higher transformation efficiency levels and rates of transformed plant recovery, thus enhancing studies of soybean abiotic conditions or interactions between phytopathogens, such as nematodes. A 55 % transformation efficiency was obtained following the addition of an acclimation step that involves hydroponics and different selection processes. The present study also validated the reference genes GmELF1-b and GmELF1-a as the most stable to be used in RT-qPCR analysis i... Mostrar Tudo |
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Thesagro: |
Soja. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02326naa a2200241 a 4500
001 2031887
005 2017-08-05
008 2015 bl uuuu u00u1 u #d
022 $a1432-203X
024 7 $a10.1007/s00299-015-1845-2$2DOI
100 1 $aKUMA, K. M.
245 $aA high efficient protocol for soybean root transformation by Agrobacterium rhizogenes and most stable reference genes for RT-qPCR analysis.$h[electronic resource]
260 $c2015
520 $aGene functional analyses are essential to the validation of results obtained from in silico and/or geneprospecting studies. Genetic transformation methods that yield tissues of transient expression quickly have been of considerable interest to researchers. Agrobacterium rhizogenes-mediated transformation methods, which are employed to generate plants with transformed roots, have proven useful for the study of stress caused by root phytopathogens via gene overexpression and/or silencing. While some protocols have been adapted to soybean plants, transformation efficiencies remain limited; thus, few viable plants are available for performing bioassays. Furthermore, mRNA analyses that employ reverse transcription quantitative polymerase chain reactions (RT-qPCR) require the use of reference genes with stable expression levels across different organs, development steps and treatments. In the present study, an A. rhizogenes-mediated soybean root transformation approach was optimized. The method delivers significantly higher transformation efficiency levels and rates of transformed plant recovery, thus enhancing studies of soybean abiotic conditions or interactions between phytopathogens, such as nematodes. A 55 % transformation efficiency was obtained following the addition of an acclimation step that involves hydroponics and different selection processes. The present study also validated the reference genes GmELF1-b and GmELF1-a as the most stable to be used in RT-qPCR analysis in composite plants, mainly under nematode infection.
650 $aSoja
700 1 $aLOPES-CAITAR, V. S.
700 1 $aROMERO, C. C. T.
700 1 $aSILVA, S. M. H.
700 1 $aCARVALHO, M. C. C. G.
700 1 $aABDELNOOR, R. V.
700 1 $aDIAS, W. P.
700 1 $aMARCELINO-GUIMARÃES, F. C.
773 $tPlant Cell Reports$gv. 34, n. 11, p. 1987-2000, 2015.
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Embrapa Soja (CNPSO) |
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