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| Registros recuperados : 189 | |
| 141. |  | PARMEZAN, T. R.; RINCÃO, M. P.; SABINO, F. C.; BRITO JUNIOR, S. L. B.; BIRKETT, M.; PICKETT, J.; HOFFMANN-CAMPO, C. B.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. Expression profile of genes involved in terpenoid biosynthesis under Phakopsora pachryrhizi infection. In: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 11., 2015, Iguassu Falls. Abstract... Abstract: 620.pdf.| Biblioteca(s): Embrapa Soja. |
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| 142. | | SANTOS, J. V. M. dos; VALLIYODAN, B.; JOSHI, T.; KHAN, S. M.; LIU, YANG.; WANG, J.; VUONG, T. D.; OLIVEIRA, M. F. de; MARCELINO-GUIMARÃES, F. C.; XU, D.; NGUYEN, H. T.; ABDELNOOR, R. V. Evaluation of genetic variation among Brazilian soybean cultivars through genome resequencing. BMC Genomics, v. 17, n. 110, 18 p., Feb. 2016.| Biblioteca(s): Embrapa Soja. |
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| 143. | | FREITAS-VANZO, A. T. de; SILVA, C. de C. da; NOVAES, T. G. de; WALZ, D. M.; MARCELINO-GUIMARÃES, F. C.; KUWAHARA, M. K.; MOLINA, R. de O.; LEITE JUNIOR, R. P. Evaluation of disease severity caused by Bean golden mosaic virus in different bean cultivars. Canadian Journal of Plant Pathology, v. 43, N. 1, p. 172-178, 2021.| Biblioteca(s): Embrapa Soja. |
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| 144. | | DARBEN, L. M.; YOKOYAMA, A.; PARMEZAN, T. R.; KUWAHARA, M. K.; CARVALHO, M. C. C. G. de; GONELA, A.; SOARES, R. M.; ALMEIDA, A. M. R.; GODOY, C. V.; MARCELINO-GUIMARÃES, F. C. Evaluation of virulence of Phakopsora pachyrhizi monourediniais isolates collected in Brazil. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 47.; SIMPÓSIO BRASILEIRO DE MOFO BRANCO, 2014, Londrina. Desafios futuros: anais. Londrina: SBF, 2014. 1 CD-ROM.| Biblioteca(s): Embrapa Soja. |
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| 145. | | DARBEN, L. M.; YOKOYAMA, A.; PARMEZAN, T. R.; LOPES-CAITAR, V. S.; AOYAGI, L. N.; CARVALHO, M. C. C. G. de; GODOY, C. V.; GONELA, A.; SOARES, R. M.; ALMEIDA, A. M. R.; MARCELINO-GUIMARÃES, F. C. Molecular diversity of monouredinial Phakopsora pachyrhizi isolates collected in Brazil. In: WORKSHOP ON BIOTIC AND ABIOTIC STRESS TOLERANCE IN PLANTS, 2013, Ilhéus. The challenge for the 21st century: book of abstracts. [Brasília]: Embrapa: International Advanced Biology Consortium, 2013. p. 56.| Biblioteca(s): Embrapa Soja. |
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| 146. | | BARROS, L. G.; AVELINO, B. B.; SILVA, D. L. L. F.; FERREIRA, E. G. C.; BISSI, R. B.; CASTANHO, F. M.; SILVA, D. C. G. da; MARCELINO-GUIMARÃES, F. C. Mapping of the resistance gene to Asian soybean rust in PI 594756 (Mapeamento do gene de resistência à ferrugem-asiática da soja na PI 594756) In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 51., 2019, Recife. Anais... Brasília, DF: SBF, 2019. p. 141.| Biblioteca(s): Embrapa Soja. |
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| 147. | | BRITTO-KIDO, S. de A.; FERREIRA NETO, J. R. C.; PANDOLFI, V.; MARCELINO-GUIMARÃES, F. C.; NEPOMUCENO, A. L.; ABDELNOOR, R. V.; BENKO-ISEPPON, A. M.; KIDO, E. A. Natural antisense transcripts in plants: a review and identification in soybean infected with Phakopsora pachyrhizi SuperSAGE Library. The Scientific World Journal, v. 2013, 14 p., 2013.| Biblioteca(s): Embrapa Soja. |
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| 148. | | RINCÃO, M. P.; CARVALHO, M. C. da C G. de; NASCIMENTO, L. C.; LOPES-CAITAR, V. S.; CARVALHO, K. de; DARBEN, L. M.; YOKOYAMA, A.; CARAZZOLLE, M. F.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. New insights into Phakopsora pachyrhizi infection based on transcriptome analysis in planta. Genetics and Molecular Biology, v. 41, n. 3, p. 671-691, 2018.| Biblioteca(s): Embrapa Soja. |
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| 149. | | CARDOSO, R.; SILVA, D. C. G. da; SANTANA, L.; LOPES, I. de O. N.; OLIVEIRA, M. F. de; ARIAS, C. A. A.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. A new locus associated with resistance to bacterial pustule caused by Xanthomonas citri pv. glycines in soybean. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 12., 2023, Caxambu, MG. Anais. Piracicaba: Sociedade Brasileira de Melhoramento de Plantas, 2023. resumo.| Biblioteca(s): Embrapa Soja. |
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| 150. | | BRUMER, B. B.; LOPES-CAITAR, V. S.; CHICOWKSI, A. S.; BELOTI, J. D.; MARIN, S. R. R.; SEIXAS, C. D. S.; SOARES, R. M.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. Morphological and molecular characterization of Diaporthe aspalathi isolates and reaction of soybean genotypes to stem canker. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 6., 2017, Ouro Preto. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2017. não paginado.| Biblioteca(s): Embrapa Soja. |
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| 151. | | BRUMER, B. B.; LOPES-CAITAR, V. S.; CHICOWSKI, A. S.; BELOTI, J. D.; MARIN, S. R. R.; SEIXAS, C. D. S.; SOARES, R. M.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. Morphological and molecular characterization of Diaporthe aspalathi isolates and reaction of soybean genotypes to stem canker. In: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 11., 2015, Iguassu Falls. Abstract... Abstract: 438.pdf.| Biblioteca(s): Embrapa Soja. |
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| 152. | | SILVA, M. L. da; PINTO, D. L. P.; PASSOS, A. B.; MARCELINO-GUIMARÃES, F. C.; ROSSI, A. A. B.; KRAUSE, W.; CARVALHO, I. F. de; BATISTA, D. S.; ROCHA, D. I.; OTONI, W. C. Novel and efficient transformation of wild passion fruit (Passiflora cincinnata Mast.) using sonication-assisted Agrobacterium-mediated transformation. In vitro cellular & developmental biology - plant, v. 1, p. 1-10, 2020.| Biblioteca(s): Embrapa Soja. |
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| 153. | | CARVALHO, J. de F. C.; CRUSIOL, L. G. T.; PERINI, L. J.; SIBALDELLI, R. N. R.; FERREIRA. L. C.; MARCELINO-GUIMARÃES, F. C.; NEPOMUCENO, A. L.; NEUMAIER, N.; FARIAS, J. R. B. Phenotyping soybeans for drought responses using remote sensing techniques and non-destructive physiological analysis. Global Science and Technology, v. 8, n. 2, p. 1-16, maio/ago. 2015.| Biblioteca(s): Embrapa Soja. |
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| 154. | | BARROS, V. de A.; FONTESA, P. P.; SOUZA, G. B. de; GONÇALVES, A. B.; CARVALHO, K. de; RINCÃO, M. P.; LOPES, I. de O. N.; COSTA, M. D. L.; ALVES, M. S.; MARCELINO-GUIMARÃES, F. C.; FIETTO, L. G. Phakopsora pachyrhizi triggers the jasmonate signaling pathway during compatible interaction in soybean and GmbZIP89 plays a role of major component in the pathway. Plant physiology and biochemistry, v. 151, p. 526-534, 2020.| Biblioteca(s): Embrapa Soja. |
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| 155. | | CARVALHO, M. C. D.; NASCIMENTO, L. C.; ROCHA, C. S.; CARAZZOLE, M. F.; DARBEN, L. M.; POLIZEL-PODANOSQUI, A. M.; PEREIRA, G. A.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. Prediction of the secretome and effectors candidates of Phakopsora pachyrhizi during the course of infection in soybean. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 46.; REUNIÃO BRASILEIRA DE CONTROLE BIOLÓGICO, 11., 2013, Ouro Preto. [Anais...]. [Brasília]: Sociedade Brasileira de Fitopatologia, 2013. 1 CD-ROM.| Biblioteca(s): Embrapa Soja. |
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| 156. | | NAKAYAMA, T. J.; RODRIGUES, F. A.; NEUMAIER, N.; MARCELINO-GUIMARÃES, F. C.; FARIAS, J. R. B.; OLIVEIRA, M. C. N. de; BORÉM, A.; OLIVEIRA, A. C. B. de; EMYGDIO, B. M.; NEPOMUCENO, A. L. Reference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions. Genetics and Molecular Research, Ribeirão Preto, v. 13, n. 1, p. 860-871, 2014.| Biblioteca(s): Embrapa Clima Temperado; Embrapa Soja. |
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| 157. | | MELLO, F. E. de; LOPES-CAITAR, V. S.; XAVIER-VALENCIO, S. A.; SILVA, H. P. da; FRANZENBURG, S.; MEHL, A.; ALEXANDER-VERREET, J.; BALBI-PEÑA, M. I.; MARCELINO-GUIMARÃES, F. C.; GODOY, C. V. Resistance of Corynespora cassiicola from soybean to QoI and MBC fungicides in Brazil. Plant Pathology, v. 71, p. 373-385, 2022.| Biblioteca(s): Embrapa Soja. |
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| 158. | | NEUMAIER, N.; CARVALHO, J. F. C.; ROLLA-SANTOS, A. A. P.; ENGELS, C.; FARIAS, J. R. B.; FUGANTI-PAGLIARINI, R.; MARCELINO-GUIMARÃES, F. C.; MARIN, S. R. R.; MIZOI, J.; YAMAGUCHI-SHINOZAKI, K.; NEPOMUCENO, A. L. Testing dreb genetically modified soybean plants for drought tolerance in both greenhouse and field conditions. In: WORLD SOYBEAN RESEARCH CONFERENCE, 9., 2013, Durban. [Proceedings...]. Durban: OPDT: OPOT, 2013. Abst. 216. 1 CD-ROM. WSRC.| Biblioteca(s): Embrapa Soja. |
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| 159. | | LOPES-CAITAR, V. S.; NOMURA, R. B. G.; HISHINUMA-SILVA, S. M.; CARVALHO, M. C. da C. G. de; ABDELNOOR, R. V.; DIAS, W. P.; MARCELINO-GUIMARÃES, F. C. Time Course RNA-seq Reveals Soybean Responses against Root-Lesion Nematode and Resistance Players. Plants, v. 11, 2983, 2022. 24 p.| Biblioteca(s): Embrapa Soja. |
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| 160. | | PARMEZAN, T. R.; BRITO JUNIOR, S. L.; CARVALHO, K. de; AQUINO, M. de; BIRKETT, M.; PICKETT, J.; NUNES, E. de O.; ABDELNOOR, R. V.; HOFFMANN-CAMPO, C. B.; MARCELINO-GUIMARÃES, F. C. Transcriptional profile of genes involved in the production of terpenes and glyceollins in response to biotic stresses in soybean. Genetics and molecular biology, v. 43, p. 1-12, 2020.| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 189 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
09/03/2015 |
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Data da última atualização: |
28/01/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
CARVALHO, M. C. C. G. de; NASCIMENTO, L. C.; POLIZEL-PODANOSQUI, A. M.; ROCHA, C. S.; DARBEN, L. M.; LOPES-CAITAR, V. S.; CARAZZOLLE, M. F.; OLIVEIRA, M. L. C. S.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. |
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Afiliação: |
UENP; UNICAMP; UNICAMP; UNESP; RICARDO VILELA ABDELNOOR, CNPSO; FRANCISMAR CORREA MARCELINO GUIMARA, CNPSO. |
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Título: |
The transcriptome interactions between Phakopsora pachykopsora pachyrhizi-soybean. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 47.; SIMPÓSIO BRASILEIRO DE MOFO BRANCO, 2014, Londrina. Desafios futuros: anais. Londrina: SBF, 2014. 1 CD-ROM. |
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Idioma: |
Inglês |
|
Conteúdo: |
The soybean rust caused by the fungus Phakopsora pachyrhizi, has caused serious damage to soybean culture since 2001 in Brazil. Several sources of rust resistance have already been identified; however no cultivar has shown stable resistance to rust. Plants are commonly infected by one or more strains of the fungus causing the breakdown of resistance acquired with the introduction of only one or a few resistance genes. In the last years, different study strategies have allowed the simultaneous monitoring of gene expression in plant-pathogen interaction, broadening our understanding of the molecular mechanisms underlying compatibility and incompatibility responses of soybean to P. pachyrhizi creating new perspectives for the development of a more durable resistance. In this work, we used laser capture microdissection (LCM) to isolate the foliar mesophyll cells of rust infection sites and access site specific processes and regulators in tolerant (compatible interaction) (BRS231) and resistant (incompatible interaction) (PI561356) soybean genotypes. RNA was extracted from the isolated cells, amplified, and sequenced with Solexa plataform. The generated paired-end sequences (54 bp) were mapped to the soybean genome and gene models (http://www.phytozome.net) for the identification of expressed genes and splicing variants. A total of 28,572 and 30,743 genes (RPKM>3) were identified for BRS231 and PI561356, respectively. The remaining reads were used to perform an ab initio assembly of P. pachyrhizi transcripts expressed at 10 dpi in planta. To improve the quality of assembly, P. pachyrhizi sequences from Sanger sequencing reads available at NCBI were trimmed and assembled into contigs and singlets. The two assemblies were merged to form PPGC1.0 comprising 36,350 unique P. pachyrhizi sequences (unisequences) expressed at 10 dpi in planta. By combining LCM with a high performance sequencing (RNA-seq) we were able to access the expression profile of a large number of genes in rust infection sites, including those related to the fungal invasion, and access new transcripts involved in soybean-rust interaction in both, compatible and incompatible interaction. Among the genes identified, the 100 soybean most expressed transcripts (RPKM) in PI561356 and BRS231 rust infection sites were stress-induced genes like cystein proteases, metallothioneins, transcription factors and genes related to protein degradation. Functional annotation of P. pachyrhizi expressed genes was done using the Blast2GO tool resulting in 3,976 transcripts associated with Gene Ontology (GO) terms, and 2,902 to biological process ontology. Biological process ontology was mainly represented by three classes of parent terms: cellular component organization or biogenesis, localization and cellular process. In the last one, two terms: response to stimulus and metabolic process were the most enriched biological process categories. Phakopsora pachyrhizi metabolic processes were strongly represented by sequences with probable function in glycogen and lipid catabolism (debranching enzymes), carbohydrate metabolism (invertases, mannitol dehydrogenases) and nitrogen metabolism (trehalose 6-phosphate synthase). Among sequences associated to response to stimulus were a great number of GO terms also linked to the symbiosis, encompassing mutualism through parasitism term (GO:0044403) including proteins involved in nucleotide binding and DNA metabolic process (nucleases and response to DNA damage stimulus), thiamin metabolic process, proteolysis, response to reactive oxygen species, GTPase mediated signal transduction, inositol-lipid mediated signaling and calmodulin signaling. Using a combination of approaches based on Blast similarities against local databases and an initio prediction, we generated a list of 905 putative secreted proteins from the total of 36,350 unique P. pachyrhizi unisequences. To identify a set of candidate P. pachyrhizi effectors, we grouped the predicted secretome into tribes or families to find those unisequences associated to expanded families. Of the 100 tribes formed, 50 of them contained more than three unisequences. A list of the 169 unisequences were obtained, that combine defining features to be common between effectors, such as small open reading frame ORF size (<250aa), no intracellular function described, enrichement of cystein residues (>3%), and presence of the previously described Y/F/WxC motif. These characteristics are mostly observed among the members of tribes 1, 2 and 17. A temporal expression of 58 candidate effector genes was characterized from spores, germinated spores and infected soybean leaves at 0, 6, 12, 24, 36, 48, 72, 96, 192 and 240 hours after inoculation (hai) using RT-qPCR. Expression analysis showed differential temporal gene expression of these candidate effectors. Heat map analysis revealed three main clusters with coordinated expression of candidates across the infection cycle. The first has candidates expressed in specific steps of infection and almost sequences have motifs common of the effectors as Y/F/WxC. Second cluster has candidates expressed during initial steps of infection that matches germ tube and appressoria formation (0 ? 24 hai) and almost sequences have ?3 cysteine residues, other common features of the effectors. Third cluster has candidates expressed only in contact with the host, suggesting that these candidates are host-induced. This last cluster has a sequence with CFEM domain associated with pathogenicity. Functional analyses are in progress with more promising candidates effectors from second and third cluster to better understand this potential effectors in soybean rust. MenosThe soybean rust caused by the fungus Phakopsora pachyrhizi, has caused serious damage to soybean culture since 2001 in Brazil. Several sources of rust resistance have already been identified; however no cultivar has shown stable resistance to rust. Plants are commonly infected by one or more strains of the fungus causing the breakdown of resistance acquired with the introduction of only one or a few resistance genes. In the last years, different study strategies have allowed the simultaneous monitoring of gene expression in plant-pathogen interaction, broadening our understanding of the molecular mechanisms underlying compatibility and incompatibility responses of soybean to P. pachyrhizi creating new perspectives for the development of a more durable resistance. In this work, we used laser capture microdissection (LCM) to isolate the foliar mesophyll cells of rust infection sites and access site specific processes and regulators in tolerant (compatible interaction) (BRS231) and resistant (incompatible interaction) (PI561356) soybean genotypes. RNA was extracted from the isolated cells, amplified, and sequenced with Solexa plataform. The generated paired-end sequences (54 bp) were mapped to the soybean genome and gene models (http://www.phytozome.net) for the identification of expressed genes and splicing variants. A total of 28,572 and 30,743 genes (RPKM>3) were identified for BRS231 and PI561356, respectively. The remaining reads were used to perform an ab initio assembly... Mostrar Tudo |
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Thesagro: |
Doença de Planta; Ferrugem; Soja. |
|
Thesaurus NAL: |
Plant diseases and disorders; Soybean rust; Soybeans. |
|
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/119824/1/PALESTRA18.pdf
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Marc: |
LEADER 06688nam a2200289 a 4500
001 2010927
005 2019-01-28
008 2014 bl uuuu u00u1 u #d
100 1 $aCARVALHO, M. C. C. G. de
245 $aThe transcriptome interactions between Phakopsora pachykopsora pachyrhizi-soybean.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 47.; SIMPÓSIO BRASILEIRO DE MOFO BRANCO, 2014, Londrina. Desafios futuros: anais. Londrina: SBF, 2014. 1 CD-ROM.$c2014
520 $aThe soybean rust caused by the fungus Phakopsora pachyrhizi, has caused serious damage to soybean culture since 2001 in Brazil. Several sources of rust resistance have already been identified; however no cultivar has shown stable resistance to rust. Plants are commonly infected by one or more strains of the fungus causing the breakdown of resistance acquired with the introduction of only one or a few resistance genes. In the last years, different study strategies have allowed the simultaneous monitoring of gene expression in plant-pathogen interaction, broadening our understanding of the molecular mechanisms underlying compatibility and incompatibility responses of soybean to P. pachyrhizi creating new perspectives for the development of a more durable resistance. In this work, we used laser capture microdissection (LCM) to isolate the foliar mesophyll cells of rust infection sites and access site specific processes and regulators in tolerant (compatible interaction) (BRS231) and resistant (incompatible interaction) (PI561356) soybean genotypes. RNA was extracted from the isolated cells, amplified, and sequenced with Solexa plataform. The generated paired-end sequences (54 bp) were mapped to the soybean genome and gene models (http://www.phytozome.net) for the identification of expressed genes and splicing variants. A total of 28,572 and 30,743 genes (RPKM>3) were identified for BRS231 and PI561356, respectively. The remaining reads were used to perform an ab initio assembly of P. pachyrhizi transcripts expressed at 10 dpi in planta. To improve the quality of assembly, P. pachyrhizi sequences from Sanger sequencing reads available at NCBI were trimmed and assembled into contigs and singlets. The two assemblies were merged to form PPGC1.0 comprising 36,350 unique P. pachyrhizi sequences (unisequences) expressed at 10 dpi in planta. By combining LCM with a high performance sequencing (RNA-seq) we were able to access the expression profile of a large number of genes in rust infection sites, including those related to the fungal invasion, and access new transcripts involved in soybean-rust interaction in both, compatible and incompatible interaction. Among the genes identified, the 100 soybean most expressed transcripts (RPKM) in PI561356 and BRS231 rust infection sites were stress-induced genes like cystein proteases, metallothioneins, transcription factors and genes related to protein degradation. Functional annotation of P. pachyrhizi expressed genes was done using the Blast2GO tool resulting in 3,976 transcripts associated with Gene Ontology (GO) terms, and 2,902 to biological process ontology. Biological process ontology was mainly represented by three classes of parent terms: cellular component organization or biogenesis, localization and cellular process. In the last one, two terms: response to stimulus and metabolic process were the most enriched biological process categories. Phakopsora pachyrhizi metabolic processes were strongly represented by sequences with probable function in glycogen and lipid catabolism (debranching enzymes), carbohydrate metabolism (invertases, mannitol dehydrogenases) and nitrogen metabolism (trehalose 6-phosphate synthase). Among sequences associated to response to stimulus were a great number of GO terms also linked to the symbiosis, encompassing mutualism through parasitism term (GO:0044403) including proteins involved in nucleotide binding and DNA metabolic process (nucleases and response to DNA damage stimulus), thiamin metabolic process, proteolysis, response to reactive oxygen species, GTPase mediated signal transduction, inositol-lipid mediated signaling and calmodulin signaling. Using a combination of approaches based on Blast similarities against local databases and an initio prediction, we generated a list of 905 putative secreted proteins from the total of 36,350 unique P. pachyrhizi unisequences. To identify a set of candidate P. pachyrhizi effectors, we grouped the predicted secretome into tribes or families to find those unisequences associated to expanded families. Of the 100 tribes formed, 50 of them contained more than three unisequences. A list of the 169 unisequences were obtained, that combine defining features to be common between effectors, such as small open reading frame ORF size (<250aa), no intracellular function described, enrichement of cystein residues (>3%), and presence of the previously described Y/F/WxC motif. These characteristics are mostly observed among the members of tribes 1, 2 and 17. A temporal expression of 58 candidate effector genes was characterized from spores, germinated spores and infected soybean leaves at 0, 6, 12, 24, 36, 48, 72, 96, 192 and 240 hours after inoculation (hai) using RT-qPCR. Expression analysis showed differential temporal gene expression of these candidate effectors. Heat map analysis revealed three main clusters with coordinated expression of candidates across the infection cycle. The first has candidates expressed in specific steps of infection and almost sequences have motifs common of the effectors as Y/F/WxC. Second cluster has candidates expressed during initial steps of infection that matches germ tube and appressoria formation (0 ? 24 hai) and almost sequences have ?3 cysteine residues, other common features of the effectors. Third cluster has candidates expressed only in contact with the host, suggesting that these candidates are host-induced. This last cluster has a sequence with CFEM domain associated with pathogenicity. Functional analyses are in progress with more promising candidates effectors from second and third cluster to better understand this potential effectors in soybean rust.
650 $aPlant diseases and disorders
650 $aSoybean rust
650 $aSoybeans
650 $aDoença de Planta
650 $aFerrugem
650 $aSoja
700 1 $aNASCIMENTO, L. C.
700 1 $aPOLIZEL-PODANOSQUI, A. M.
700 1 $aROCHA, C. S.
700 1 $aDARBEN, L. M.
700 1 $aLOPES-CAITAR, V. S.
700 1 $aCARAZZOLLE, M. F.
700 1 $aOLIVEIRA, M. L. C. S.
700 1 $aABDELNOOR, R. V.
700 1 $aMARCELINO-GUIMARÃES, F. C.
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