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Registro Completo |
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
23/05/2001 |
Data da última atualização: |
30/08/2018 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
MOURA, M. C. C. L.; QUEIROZ, M. A. de. |
Afiliação: |
UEMA; MANOEL ABÍLIO DE QUEIROZ, CPATSA. |
Título: |
Variabilidade dos acessos de cucurbitaceae coletados em 10 regioes do estado do Maranhao. |
Ano de publicação: |
1997 |
Fonte/Imprenta: |
Horticultura Brasileira, Brasília, DF, v. 15, 1997. |
Idioma: |
Português |
Notas: |
Resumo 188. Suplemento. Edição dos Resumos do 37 Congresso Brasileiro de Olericultura, Manaus, 1997. |
Conteúdo: |
O presente trabalho leve como objetivo ampliar a variabilidade genética das cucurbatáceas a partir da coleta de populacoes locais através de amostras de frutos e/ou sementes. |
Palavras-Chave: |
Coleta; Cucurbitacea; Variabilidade genetica. |
Thesagro: |
Cucurbitaceae; Hortaliça Cucurbitácea. |
Thesaurus Nal: |
gene banks; genetic variation. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182151/1/Horticultura-Brasileira-v.15-resumo-188-1997.pdf
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Marc: |
LEADER 00908nam a2200217 a 4500 001 1134176 005 2018-08-30 008 1997 bl uuuu u00u1 u #d 100 1 $aMOURA, M. C. C. L. 245 $aVariabilidade dos acessos de cucurbitaceae coletados em 10 regioes do estado do Maranhao. 260 $aHorticultura Brasileira, Brasília, DF, v. 15, 1997.$c1997 500 $aResumo 188. Suplemento. Edição dos Resumos do 37 Congresso Brasileiro de Olericultura, Manaus, 1997. 520 $aO presente trabalho leve como objetivo ampliar a variabilidade genética das cucurbatáceas a partir da coleta de populacoes locais através de amostras de frutos e/ou sementes. 650 $agene banks 650 $agenetic variation 650 $aCucurbitaceae 650 $aHortaliça Cucurbitácea 653 $aColeta 653 $aCucurbitacea 653 $aVariabilidade genetica 700 1 $aQUEIROZ, M. A. de
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
24/03/2009 |
Data da última atualização: |
16/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
Nacional - A |
Autoria: |
MARCELINO, F. C.; GUIMARÃES, M. F. M.; BARROS, E. G. |
Afiliação: |
Francismar Correa Marcelino, Embrapa Soja; Marta Fonseca Martins Guimaraes, Embrapa Gado de Leite; Everaldo Gonçalves de Barros, Bioagro / UFV. |
Título: |
Detection and quantification of Roundup Ready soybean residues in sausage samples by conventional and real-time PCR. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Ciência e Tecnologia de Alimentos, Campinas, v. 28, p. 38-45, 2008. |
DOI: |
https://doi.org/10.1590/S0101-20612008000500007 |
Idioma: |
Inglês |
Notas: |
Supl. |
Conteúdo: |
The increasing presence of products derived from genetically modified (GM) plants in human and animal diets has led to the development of detection methods to distinguish biotechnology-derived foods from conventional ones. The conventional and real-time PCR have been used, respectively, to detect and quantify GM residues in highly processed foods. DNA extraction is a critical step during the analysis process. Some factors such as DNA degradation, matrix effects, and the presence of PCR inhibitors imply that a detection or quantification limit, established for a given method, is restricted to a matrix used during validation and cannot be projected to any other matrix outside the scope of the method. In Brazil, sausage samples were the main class of processed products in which Roundup Ready® (RR) soybean residues were detected. Thus, the validation of methodologies for the detection and quantification of those residues is absolutely necessary. Sausage samples were submitted to two different methods of DNA extraction: modified Wizard and the CTAB method. The yield and quality were compared for both methods. DNA samples were analyzed by conventional and real-time PCR for the detection and quantification of Roundup Ready® soybean in the samples. At least 200 ng of total sausage DNA was necessary for a reliable quantification. Reactions containing DNA amounts below this value led to large variations on the expected GM percentage value. In conventional PCR, the detection limit varied from 1.0 to 500 ng, depending on the GM soybean content in the sample. The precision, performance, and linearity were relatively high indicating that the method used for analysis was satisfactory. MenosThe increasing presence of products derived from genetically modified (GM) plants in human and animal diets has led to the development of detection methods to distinguish biotechnology-derived foods from conventional ones. The conventional and real-time PCR have been used, respectively, to detect and quantify GM residues in highly processed foods. DNA extraction is a critical step during the analysis process. Some factors such as DNA degradation, matrix effects, and the presence of PCR inhibitors imply that a detection or quantification limit, established for a given method, is restricted to a matrix used during validation and cannot be projected to any other matrix outside the scope of the method. In Brazil, sausage samples were the main class of processed products in which Roundup Ready® (RR) soybean residues were detected. Thus, the validation of methodologies for the detection and quantification of those residues is absolutely necessary. Sausage samples were submitted to two different methods of DNA extraction: modified Wizard and the CTAB method. The yield and quality were compared for both methods. DNA samples were analyzed by conventional and real-time PCR for the detection and quantification of Roundup Ready® soybean in the samples. At least 200 ng of total sausage DNA was necessary for a reliable quantification. Reactions containing DNA amounts below this value led to large variations on the expected GM percentage value. In conventional PCR, the detection limit vari... Mostrar Tudo |
Palavras-Chave: |
GMO; PCR quantitative; Sausage; Transgenic residues. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/596463/1/Detection-and-quantification-of-Roundup-Ready-soybean.pdf
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Marc: |
LEADER 02405naa a2200217 a 4500 001 1596463 005 2024-02-16 008 2008 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1590/S0101-20612008000500007$2DOI 100 1 $aMARCELINO, F. C. 245 $aDetection and quantification of Roundup Ready soybean residues in sausage samples by conventional and real-time PCR.$h[electronic resource] 260 $c2008 500 $aSupl. 520 $aThe increasing presence of products derived from genetically modified (GM) plants in human and animal diets has led to the development of detection methods to distinguish biotechnology-derived foods from conventional ones. The conventional and real-time PCR have been used, respectively, to detect and quantify GM residues in highly processed foods. DNA extraction is a critical step during the analysis process. Some factors such as DNA degradation, matrix effects, and the presence of PCR inhibitors imply that a detection or quantification limit, established for a given method, is restricted to a matrix used during validation and cannot be projected to any other matrix outside the scope of the method. In Brazil, sausage samples were the main class of processed products in which Roundup Ready® (RR) soybean residues were detected. Thus, the validation of methodologies for the detection and quantification of those residues is absolutely necessary. Sausage samples were submitted to two different methods of DNA extraction: modified Wizard and the CTAB method. The yield and quality were compared for both methods. DNA samples were analyzed by conventional and real-time PCR for the detection and quantification of Roundup Ready® soybean in the samples. At least 200 ng of total sausage DNA was necessary for a reliable quantification. Reactions containing DNA amounts below this value led to large variations on the expected GM percentage value. In conventional PCR, the detection limit varied from 1.0 to 500 ng, depending on the GM soybean content in the sample. The precision, performance, and linearity were relatively high indicating that the method used for analysis was satisfactory. 653 $aGMO 653 $aPCR quantitative 653 $aSausage 653 $aTransgenic residues 700 1 $aGUIMARÃES, M. F. M. 700 1 $aBARROS, E. G. 773 $tCiência e Tecnologia de Alimentos, Campinas$gv. 28, p. 38-45, 2008.
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Embrapa Gado de Leite (CNPGL) |
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