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Registros recuperados : 4 | |
1. | | MARGARIDO, A.; HERRMANN JUNIOR, P. S. de P.; MANZINE, L. R.; ARAUJO-MOREIRA, F. M. A selective VOC biosensor based on microcantilever functionalized with enzyme. Potential application on plant investigations. In: II Latin-American Conference on Plant Phenotyping and Phenomics for Plant Breeding, 2, 2017, São Carlos, SP. Proceedings... São Carlos: Embrapa Instrumentação, p. 53, 2017. Biblioteca(s): Embrapa Instrumentação. |
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4. | | COLUSSI, F.; SERPA, V.; DELABONA, P. da S.; MANZINE, L. R.; VOLTATÓDIO, M. L.; ALVES, R.; MELLO, B. L.; PEREIRA JUNIOR, N.; FARINAS, C. S.; GOLUBEV, A. M.; SANTOS, M. A. M.; POLIKARPOV, I. Purification, and biochemical and biophysical characterization of cellobiohydrolase I from trichoderma harzianum IOC 3844. Journal of microbiology and biotechnology, Kangnam-Gu, v. 21, n. 8, p. 808-817, 2011. Biblioteca(s): Embrapa Instrumentação. |
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Registros recuperados : 4 | |
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Registro Completo
Biblioteca(s): |
Embrapa Instrumentação. |
Data corrente: |
17/09/2021 |
Data da última atualização: |
10/06/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
C - 0 |
Autoria: |
MARGARIDO, A.; MANZINE, L. R.; ARAUJO-MOREIRA, F. M.; GONÇALVES, R. V.; HERRMANN JUNIOR, P. S. de P. |
Afiliação: |
PAULO SERGIO DE P HERRMANN JUNIOR, CNPDIA. |
Título: |
Development of an enzyme-coated microcantilever-based biosensor for specific detection of short-chain alcohols. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Engineering Proceedings, v. 6, n. 75 2021. |
Páginas: |
1 - 5 |
ISSN: |
2359-1757 |
DOI: |
https://doi.org/10.3390/I3S2021Dresden-10175 |
Idioma: |
Inglês |
Notas: |
Presented at the 8th International Symposium on Sensor Science. |
Conteúdo: |
This paper describes the development of a biosensor designed for the enzymatic detection of short-chain alcohols. The biorecognition element, alcohol dehydrogenase, was immobilized on selfassembled monolayers deposited on top of silicon nitride microcantilevers. The self-assembly process was performed by surface activation using 3-aminopropyltriethoxysilane, followed by glutaraldehyde and biomolecule binding. X-ray photoelectron spectroscopy and atomic force microscopy were used. The biosensor showed a lower response time and sensibility from 0.03 to 1.2 mL/L. Its selectivity was analyzed through exposure to pure and mixed volatile solvents. Sensor sensibility was higher in the presence of short-chain alcohols and practically null involving other polar or nonpolar solvents. |
Palavras-Chave: |
Biorecognition element; Biosensor; Microcantilever. |
Categoria do assunto: |
W Química e Física |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/226141/1/P-Development-of-an-Enzyme-Coated-Microcantilever-Based-....pdf
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Marc: |
LEADER 01623naa a2200253 a 4500 001 2134534 005 2022-06-10 008 2021 bl uuuu u00u1 u #d 022 $a2359-1757 024 7 $ahttps://doi.org/10.3390/I3S2021Dresden-10175$2DOI 100 1 $aMARGARIDO, A. 245 $aDevelopment of an enzyme-coated microcantilever-based biosensor for specific detection of short-chain alcohols.$h[electronic resource] 260 $c2021 300 $a1 - 5 500 $aPresented at the 8th International Symposium on Sensor Science. 520 $aThis paper describes the development of a biosensor designed for the enzymatic detection of short-chain alcohols. The biorecognition element, alcohol dehydrogenase, was immobilized on selfassembled monolayers deposited on top of silicon nitride microcantilevers. The self-assembly process was performed by surface activation using 3-aminopropyltriethoxysilane, followed by glutaraldehyde and biomolecule binding. X-ray photoelectron spectroscopy and atomic force microscopy were used. The biosensor showed a lower response time and sensibility from 0.03 to 1.2 mL/L. Its selectivity was analyzed through exposure to pure and mixed volatile solvents. Sensor sensibility was higher in the presence of short-chain alcohols and practically null involving other polar or nonpolar solvents. 653 $aBiorecognition element 653 $aBiosensor 653 $aMicrocantilever 700 1 $aMANZINE, L. R. 700 1 $aARAUJO-MOREIRA, F. M. 700 1 $aGONÇALVES, R. V. 700 1 $aHERRMANN JUNIOR, P. S. de P. 773 $tEngineering Proceedings$gv. 6, n. 75 2021.
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Embrapa Instrumentação (CNPDIA) |
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