| |
|
|
| Registros recuperados : 1 | |
| Registros recuperados : 1 | |
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Acre. |
|
Data corrente: |
11/06/2014 |
|
Data da última atualização: |
06/07/2021 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 1 |
|
Autoria: |
SILVA, C. C.; MANTELLO, C. C.; CAMPOS, T. de; SOUZA, L. M.; GONÇALVES, P. S.; SOUZA, A. P. |
|
Afiliação: |
Carla C. Silva, Universidade Estadual de Campinas (Unicamp); Camila C. Mantello, Universidade Estadual de Campinas (Unicamp); TATIANA DE CAMPOS, CPAF-AC; Livia M. Souza, Universidade Estadual de Campinas (Unicamp); Paulo S. Gonçalves, Instituto Agronômico de Campinas (IAC); Anete P. Souza, Universidade Estadual de Campinas (Unicamp). |
|
Título: |
Leaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions: the development of gene-targeted Functional markers for stress response. |
|
Ano de publicação: |
2014 |
|
Fonte/Imprenta: |
Molecular Breeding, Berlim, v. 34, n. 3, p. 1035-1053, Oct. 2014. |
|
ISSN: |
1380-3743 (impresso) / 1572-9788 (on-line) |
|
DOI: |
10.1007/s11032-014-0095-2 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Hevea brasiliensis is a native species of the Amazon Basin of South America and the primary source of natural rubber worldwide. Due to the occurrence of South American Leaf Blight disease in this area, rubber plantations have been extended to suboptimal regions. Rubber tree breeding is time-consuming and expensive, but molecular markers can serve as a tool for early valuation, thus reducing time and costs. In this work, we constructed six different cDNA libraries with the aim of developing gene-targeted molecular markers for the rubber tree. A total of 8,263 reads were assembled, generating 5,025 unigenes that were analyzed; 912 expressed sequence tags (ESTs) represented new transcripts, and two sequences were highly up-regulated by cold stress. These unigenes were scanned for microsatellite (SSR) regions and single nucleotide polymorphisms (SNPs). In total, 169 novel EST-SSR markers were developed; 138 loci were polymorphic in the rubber tree, and 98 % presented transferability to six other Hevea species. Locus duplication was observed in H. brasil-iensis and other species. Additionally, 43 SNP markers in 13 sequences that showed similarity to proteins involved in stress response, latex biosynthesis and developmental processes were characterized. cDNA libraries are a rich source of SSR and SNP markers and enable the identification of new transcripts. The new markers developed here will be a valuable resource for linkage mapping, QTL identification and other studies in the rubber tree and can also be used to evaluate the genetic variability of other Hevea species, which are valuable assets in rubber tree breeding. MenosHevea brasiliensis is a native species of the Amazon Basin of South America and the primary source of natural rubber worldwide. Due to the occurrence of South American Leaf Blight disease in this area, rubber plantations have been extended to suboptimal regions. Rubber tree breeding is time-consuming and expensive, but molecular markers can serve as a tool for early valuation, thus reducing time and costs. In this work, we constructed six different cDNA libraries with the aim of developing gene-targeted molecular markers for the rubber tree. A total of 8,263 reads were assembled, generating 5,025 unigenes that were analyzed; 912 expressed sequence tags (ESTs) represented new transcripts, and two sequences were highly up-regulated by cold stress. These unigenes were scanned for microsatellite (SSR) regions and single nucleotide polymorphisms (SNPs). In total, 169 novel EST-SSR markers were developed; 138 loci were polymorphic in the rubber tree, and 98 % presented transferability to six other Hevea species. Locus duplication was observed in H. brasil-iensis and other species. Additionally, 43 SNP markers in 13 sequences that showed similarity to proteins involved in stress response, latex biosynthesis and developmental processes were characterized. cDNA libraries are a rich source of SSR and SNP markers and enable the identification of new transcripts. The new markers developed here will be a valuable resource for linkage mapping, QTL identification and other studies in the r... Mostrar Tudo |
|
Palavras-Chave: |
Biblioteca cDNA; CDNA library; Fitomejoramiento; Marcador microssatélite; Marcador SNP; Marcadores genéticos; Repeticiones de microsatélite; Rubber tree. |
|
Thesagro: |
Caucho; DNA; Hevea brasiliensis; Marcador genético; Melhoramento genético vegetal; Seringueira. |
|
Thesaurus NAL: |
Genetic markers; Microsatellite repeats; Plant breeding. |
|
Categoria do assunto: |
G Melhoramento Genético |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/160345/1/25073.pdf
|
|
Marc: |
LEADER 02997naa a2200409 a 4500
001 1988256
005 2021-07-06
008 2014 bl uuuu u00u1 u #d
022 $a1380-3743 (impresso) / 1572-9788 (on-line)
024 7 $a10.1007/s11032-014-0095-2$2DOI
100 1 $aSILVA, C. C.
245 $aLeaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions$bthe development of gene-targeted Functional markers for stress response.$h[electronic resource]
260 $c2014
520 $aHevea brasiliensis is a native species of the Amazon Basin of South America and the primary source of natural rubber worldwide. Due to the occurrence of South American Leaf Blight disease in this area, rubber plantations have been extended to suboptimal regions. Rubber tree breeding is time-consuming and expensive, but molecular markers can serve as a tool for early valuation, thus reducing time and costs. In this work, we constructed six different cDNA libraries with the aim of developing gene-targeted molecular markers for the rubber tree. A total of 8,263 reads were assembled, generating 5,025 unigenes that were analyzed; 912 expressed sequence tags (ESTs) represented new transcripts, and two sequences were highly up-regulated by cold stress. These unigenes were scanned for microsatellite (SSR) regions and single nucleotide polymorphisms (SNPs). In total, 169 novel EST-SSR markers were developed; 138 loci were polymorphic in the rubber tree, and 98 % presented transferability to six other Hevea species. Locus duplication was observed in H. brasil-iensis and other species. Additionally, 43 SNP markers in 13 sequences that showed similarity to proteins involved in stress response, latex biosynthesis and developmental processes were characterized. cDNA libraries are a rich source of SSR and SNP markers and enable the identification of new transcripts. The new markers developed here will be a valuable resource for linkage mapping, QTL identification and other studies in the rubber tree and can also be used to evaluate the genetic variability of other Hevea species, which are valuable assets in rubber tree breeding.
650 $aGenetic markers
650 $aMicrosatellite repeats
650 $aPlant breeding
650 $aCaucho
650 $aDNA
650 $aHevea brasiliensis
650 $aMarcador genético
650 $aMelhoramento genético vegetal
650 $aSeringueira
653 $aBiblioteca cDNA
653 $aCDNA library
653 $aFitomejoramiento
653 $aMarcador microssatélite
653 $aMarcador SNP
653 $aMarcadores genéticos
653 $aRepeticiones de microsatélite
653 $aRubber tree
700 1 $aMANTELLO, C. C.
700 1 $aCAMPOS, T. de
700 1 $aSOUZA, L. M.
700 1 $aGONÇALVES, P. S.
700 1 $aSOUZA, A. P.
773 $tMolecular Breeding, Berlim$gv. 34, n. 3, p. 1035-1053, Oct. 2014.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Acre (CPAF-AC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
