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| Registros recuperados : 13 | |
| 3. |  | MANTELL, S. H.; HUGO, S. A. Effects of photoperiod, mineral medium strength, inorganic ammonium, sucrose and cytokinin on root, shoot and microtuber development in shoot cultures of Dioscorea alata L. and dulbifera L. yams. Plant Cell, Tissue and Organ Culture, v.16, p.23-37, 1989.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 4. | | MANTELL, S. H.; HUGO, S. A. Effects of photoperiod, mineral medium strength, inorganic ammonium, sucrose and cytokinin on root, shoot and microtuber developmet in shoot cultures of dioscorea alata L. and D. bulbifera L. yams. Plant Cell, Tissue and Organ Culture, v.16, p.23-37, 1989.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 13. | | MONTEIRO, A. L. C.; VIEIRA, I. M. S.; BARBOSA, A. do S. A.; CONCEIÇÃO, H. E. O.; BOTELHO, M. do N.; SANTOS, D. S. B. dos; MANTELL, S. H. Resposta in vitro de ápices caulinares e segmentos nodais de Carapa guianensis Aublet. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA FCAP, 11.; SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA ORIENTAL (AVALIAÇÃO-2001), 5., 2001, Belém, PA. Resumos. Belém, PA: FCAP: Embrapa Amazônia Oriental, 2002. p. 84-86.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| Registros recuperados : 13 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
22/08/2001 |
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Data da última atualização: |
27/09/2004 |
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Autoria: |
MOURA-COSA, P.H.; MANTELL, S. H. |
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Título: |
A Preliminary Evaluation of Cell and Tissue Culture Methods Suitable for Screening Anthrocnose Disease Reactions in Tropical Yams. |
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Ano de publicação: |
1993 |
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Fonte/Imprenta: |
Pesquisa Agropecuaria Brasileira, Brasilia, v.28, n.11, p.1297-1306,nov.1993 |
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Idioma: |
Inglês |
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Conteúdo: |
Effect of different temperatures (15, 19, 25 and 29 °C), media type (PDA and Czapek Dox agar) and light on sporulation of a Caribbean pathovar of Colletotrichum gloe-osporioides f.sp. dioscoreae were studied. Czapek Dox agar and temperature of 25 °C were found to be best conditions for supporting growth and sporulation of this organism. Long-term fungal cultures (2 years axenic culture) did not sporulate effectively under the above conditions unless they were first brought into contact with living tissues of Dioscorea. Methods for inoculation of yams with conidiospores of C. gloeosporioides were developed at the plant, mi-croplant and cell levels. In vitro shoot culture clones showed a similar range of disease reactions to the pathovar as field-grown plants. D. alata showed marked sensitivity to the disease while D. esculenta was the most tolerant species followed by D. composita and D. cayenensis. Dioscorea protoplasts responded differently when cultured in the presence of mycelial culture filtrates and Czapek Dox liquid medium. Glasshouse-raised plants developed anthracnose symptoms only after wounding. |
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Palavras-Chave: |
Colletotrichum gloesporioides f; Czapek Dox aga; dioscoreae; microplant; sp. |
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Thesaurus NAL: |
clones. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/20576/1/pab11_nov_93.pdf
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Marc: |
LEADER 01763naa a2200205 a 4500
001 1105490
005 2004-09-27
008 1993 bl uuuu u00u1 u #d
100 1 $aMOURA-COSA, P.H.
245 $aA Preliminary Evaluation of Cell and Tissue Culture Methods Suitable for Screening Anthrocnose Disease Reactions in Tropical Yams.
260 $c1993
520 $aEffect of different temperatures (15, 19, 25 and 29 °C), media type (PDA and Czapek Dox agar) and light on sporulation of a Caribbean pathovar of Colletotrichum gloe-osporioides f.sp. dioscoreae were studied. Czapek Dox agar and temperature of 25 °C were found to be best conditions for supporting growth and sporulation of this organism. Long-term fungal cultures (2 years axenic culture) did not sporulate effectively under the above conditions unless they were first brought into contact with living tissues of Dioscorea. Methods for inoculation of yams with conidiospores of C. gloeosporioides were developed at the plant, mi-croplant and cell levels. In vitro shoot culture clones showed a similar range of disease reactions to the pathovar as field-grown plants. D. alata showed marked sensitivity to the disease while D. esculenta was the most tolerant species followed by D. composita and D. cayenensis. Dioscorea protoplasts responded differently when cultured in the presence of mycelial culture filtrates and Czapek Dox liquid medium. Glasshouse-raised plants developed anthracnose symptoms only after wounding.
650 $aclones
653 $aColletotrichum gloesporioides f
653 $aCzapek Dox aga
653 $adioscoreae
653 $amicroplant
653 $asp
700 1 $aMANTELL, S. H.
773 $tPesquisa Agropecuaria Brasileira, Brasilia$gv.28, n.11, p.1297-1306,nov.1993
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