Data/hora: 25/06/2024 00:56:41

Biblioteca(s): | Embrapa Café; Embrapa Unidades Centrais.
Data corrente: | 04/01/2008
Data da última atualização: | 16/03/2011
Autoria: | PINTO, F. de O.; MALUF, M. P.; GUERREIRO-FILHO, O.
Afiliação: | FERNANDA DE OLIVEIRA PINTO, IAC; MIRIAN PEREZ MALUF, SAPC; OLIVEIRO GUERREIRO-FILHO, IAC.
Título: | Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance.
Ano de publicação: | 2007
Fonte/Imprenta: | Pesquisa Agropecuária Brasileira, Brasília, v.42, n. 3, p.377-384, mar. 2007.
Idioma: | Inglês
Conteúdo: | The objective of this work was to identify expressed simple sequence repeats (SSR) markers associated to leaf miner resistance in coffee progenies. Identification of SSR markers was accomplished by directed searches on the Brazilian Coffee Expressed Sequence Tags (EST) database. Sequence analysis of 32 selected SSR loci showed that 65% repeats are of tetra-, 21% of tri- and 14% of dinucleotides. Also, expressed SSR are localized frequently in the 5'-UTR of gene transcript. Moreover, most of the genes containing SSR are associated with defense mechanisms. Polymorphisms were analyzed in progenies segregating for resistance to the leaf miner and corresponding to advanced generations of a Coffea arabica x Coffea racemosa hybrid. Frequency of SSR alleles was 2.1 per locus. However, no polymorphism associated with leaf miner resistance was identified. These results suggest that marker-assisted selection in coffee breeding should be performed on the initial cross, in which genetic variability is still significant
Palavras-Chave: | marcadores moleculares; microsatellites; microssatélites; molecular markers.
Thesagro: | Leucoptera Coffeella.
Categoria do assunto: | --

URL:

http://ainfo.cnptia.embrapa.br/digital/bitstream/item/29632/1/Study-of-simple.pdf
http://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/41010/1/42n03a11.pdf

Registro original: | Embrapa Café (CNPCa)

Exemplares:

AI-SEDE - (41010-1) - UPE - AP - PP - 630.72081 - P474 -  - DIS
CNPCa - (182-1) - UPC - AP - DD -  -  -  - DIS