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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
30/11/2021 |
Data da última atualização: |
30/11/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
GUERRA, P. A.; SOUZA, E. H. de; ANDRADE, E. C. de; MAX, D. de A. S.; OLIVEIRA, R. S.; SOUZA, F. V. D. |
Afiliação: |
PATRÍCIA ARAÚJO GUERRA, UFRB; EVERTON HILO DE SOUZA, UFRB; EDUARDO CHUMBINHO DE ANDRADE, CNPMF; DANIELA DE ANDRADE SILVA MAX, UFRB; RAFAELLE SOUZA DE OLIVEIRA, UFRB; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF. |
Título: |
Comparison of shoot tip culture and cryotherapy for eradication of ampeloviruses associated with Pineapple mealybug wilt in wild varieties |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
In Vitro Cellular & Developmental Biology - Plant, 2020. |
ISSN: |
1475-2689 |
Idioma: |
Inglês |
Conteúdo: |
The Pineapple mealybug wilt-associated virus (PMWaV) provokes a disease that causes considerable losses to pineapple growers due to the reduced leaf tissue turgescence, resulting in leaf necrosis and death in severe cases. In this work, we describe the comparison of shoot tip culture and cryotherapy methods to eradicate the pineapple wilt disease-associated ampeloviruses. Plants from the accessions Ananas comosus var. comosus(AGB-009), var. bracteatus(AGB-119), and var. parguazensis(AGB376) were indexed by RT-PCR, confirming mixed infections of PMWaV-1, PMWaV-2, and PMWaV-3. The accessions were cultured in vitro and their shoot tips treated by cryotherapy following a droplet vitrification protocol. The regeneration rate from shoot tip culture was 93% for AGB-376 and 100% for the other two accessions. After freezing, AGB-376 had 100% regeneration subsequent to exposure to PVS2 for 45 min, followed by 95% for AGB-009, while for AGB-119 the optimal exposure time was 60 min, with plant regeneration from nearly 80% of the shoot tips. For the accessions AGB-009 and AGB-376, all the recovered plants were virus free by the two methods, while 50% of the plants from accession AGB-119 remained infected. These results indicate that shoot tip culture alone or in association with cryotherapy is a promising routine method for virus removal from pineapple plant tissues and is useful to ensure that backup reserves of pineapple germplasm, conserved by in vitro bank and cryobank, are formed with virus-free plants. MenosThe Pineapple mealybug wilt-associated virus (PMWaV) provokes a disease that causes considerable losses to pineapple growers due to the reduced leaf tissue turgescence, resulting in leaf necrosis and death in severe cases. In this work, we describe the comparison of shoot tip culture and cryotherapy methods to eradicate the pineapple wilt disease-associated ampeloviruses. Plants from the accessions Ananas comosus var. comosus(AGB-009), var. bracteatus(AGB-119), and var. parguazensis(AGB376) were indexed by RT-PCR, confirming mixed infections of PMWaV-1, PMWaV-2, and PMWaV-3. The accessions were cultured in vitro and their shoot tips treated by cryotherapy following a droplet vitrification protocol. The regeneration rate from shoot tip culture was 93% for AGB-376 and 100% for the other two accessions. After freezing, AGB-376 had 100% regeneration subsequent to exposure to PVS2 for 45 min, followed by 95% for AGB-009, while for AGB-119 the optimal exposure time was 60 min, with plant regeneration from nearly 80% of the shoot tips. For the accessions AGB-009 and AGB-376, all the recovered plants were virus free by the two methods, while 50% of the plants from accession AGB-119 remained infected. These results indicate that shoot tip culture alone or in association with cryotherapy is a promising routine method for virus removal from pineapple plant tissues and is useful to ensure that backup reserves of pineapple germplasm, conserved by in vitro bank and cryobank, are formed wi... Mostrar Tudo |
Thesagro: |
Abacaxi; Planta Ornamental. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02208naa a2200217 a 4500 001 2136827 005 2021-11-30 008 2020 bl uuuu u00u1 u #d 022 $a1475-2689 100 1 $aGUERRA, P. A. 245 $aComparison of shoot tip culture and cryotherapy for eradication of ampeloviruses associated with Pineapple mealybug wilt in wild varieties$h[electronic resource] 260 $c2020 520 $aThe Pineapple mealybug wilt-associated virus (PMWaV) provokes a disease that causes considerable losses to pineapple growers due to the reduced leaf tissue turgescence, resulting in leaf necrosis and death in severe cases. In this work, we describe the comparison of shoot tip culture and cryotherapy methods to eradicate the pineapple wilt disease-associated ampeloviruses. Plants from the accessions Ananas comosus var. comosus(AGB-009), var. bracteatus(AGB-119), and var. parguazensis(AGB376) were indexed by RT-PCR, confirming mixed infections of PMWaV-1, PMWaV-2, and PMWaV-3. The accessions were cultured in vitro and their shoot tips treated by cryotherapy following a droplet vitrification protocol. The regeneration rate from shoot tip culture was 93% for AGB-376 and 100% for the other two accessions. After freezing, AGB-376 had 100% regeneration subsequent to exposure to PVS2 for 45 min, followed by 95% for AGB-009, while for AGB-119 the optimal exposure time was 60 min, with plant regeneration from nearly 80% of the shoot tips. For the accessions AGB-009 and AGB-376, all the recovered plants were virus free by the two methods, while 50% of the plants from accession AGB-119 remained infected. These results indicate that shoot tip culture alone or in association with cryotherapy is a promising routine method for virus removal from pineapple plant tissues and is useful to ensure that backup reserves of pineapple germplasm, conserved by in vitro bank and cryobank, are formed with virus-free plants. 650 $aAbacaxi 650 $aPlanta Ornamental 700 1 $aSOUZA, E. H. de 700 1 $aANDRADE, E. C. de 700 1 $aMAX, D. de A. S. 700 1 $aOLIVEIRA, R. S. 700 1 $aSOUZA, F. V. D. 773 $tIn Vitro Cellular & Developmental Biology - Plant, 2020.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca(s): |
Embrapa Clima Temperado. |
Data corrente: |
12/06/2020 |
Data da última atualização: |
12/06/2020 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
LIMA, A. D. V.; SILVA, G. F. da; MARCO, R. de; MALGARIM, M. B.; MARTINS, C. R. |
Afiliação: |
ANTÔNIO DAVI VAZ LIMA; GUILHERME FERREIRA DA SILVA; RUDINEI DE MARCO; MARCELO BARBOSA MALGARIM, UFPEL; CARLOS ROBERTO MARTINS, CPACT. |
Título: |
Inconformidade morfológica na relação porta-enxerto/cultivar copa em nogueira-pecã. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
In: SIMPÓSIO SUL-AMERICANO DA NOZ-PECÃ, 2., 2019, Cachoeira do Sul. Anais. Brasília, DF: Embrapa, 2019. 119 p. Carlos Roberto Martins, Editor técnico (CPACT). p. 113-115. |
Idioma: |
Português |
Conteúdo: |
O objetivo do trabalho foi identificar inconformidades morfológicas na relação dos diâmetros do porta-enxerto com a cultivar copa em 12 cultivares de nogueria-pecã. |
Thesagro: |
Diâmetro; Morfologia Vegetal; Noz Peca; Porta Enxerto. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/213962/1/Artigo-inconformidade.pdf
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Marc: |
LEADER 00903nam a2200205 a 4500 001 2123263 005 2020-06-12 008 2019 bl uuuu u00u1 u #d 100 1 $aLIMA, A. D. V. 245 $aInconformidade morfológica na relação porta-enxerto/cultivar copa em nogueira-pecã.$h[electronic resource] 260 $aIn: SIMPÓSIO SUL-AMERICANO DA NOZ-PECÃ, 2., 2019, Cachoeira do Sul. Anais. Brasília, DF: Embrapa, 2019. 119 p. Carlos Roberto Martins, Editor técnico (CPACT). p. 113-115.$c2019 520 $aO objetivo do trabalho foi identificar inconformidades morfológicas na relação dos diâmetros do porta-enxerto com a cultivar copa em 12 cultivares de nogueria-pecã. 650 $aDiâmetro 650 $aMorfologia Vegetal 650 $aNoz Peca 650 $aPorta Enxerto 700 1 $aSILVA, G. F. da 700 1 $aMARCO, R. de 700 1 $aMALGARIM, M. B. 700 1 $aMARTINS, C. R.
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