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| Registros recuperados : 25 | |
| 3. |  | FRÂNCIO, L.; MALABARBA, J.; BUFFON, V.; REVERS, L. F. Desenvolvimento de marcadores para a seleção assistida de ausência de sementes em videiras no programa de melhoramento da Embrapa Uva e Vinho. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 14. ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 10., 2016, Bento Gonçalves. Resumos...Bento Gonçalves, RS: Embrapa uva e Vinho, 2016. p. 56. (Embrapa Uva e Vinho. Documentos, 99)| Biblioteca(s): Embrapa Uva e Vinho. |
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| 4. | | MALABARBA, J.; BUFFON, V.; PASQUALI, G.; REVERS, L. F. Estudo estrutural e funcional do gene VvAGL11 e seu papel na morfogênese de sementes de Vitis vinifera. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA UVA E VINHO, 12., ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 8., 2014, Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2014. p. 39| Biblioteca(s): Embrapa Uva e Vinho. |
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| 5. | | WAIRICH, A.; MALABARBA, J.; BUFFON, V.; PORTO, D. D.; REVERS, L. F. Análise do perfil transcricional de genes candidatos associados à resistência ao míldio em videira, presentes no locus RPV3. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 22., 2012, Bento Gonçalves. Anais... Bento Gonçalves: SBF, p. 4332-4335, 2012.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 8. | | MARIMON, P.; FRÂNCIO, L.; MALABARBA, J.; BUFFON, V.; REVERS, L. F. Utilização de um vetor viral para expressão ectópica do gene MrRGA8 em videira. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 15., 2017.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 11., 2017, Bento Gonçalves. Resumos...Bento Gonçalves, RS: Embrapa Uva e Vinho, 2017. p. 39.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 10. | | MALABARBA, J.; WAIRICH, A.; BUFFON, V.; CZERMAINSKI, A. B. C.; REVERS, L. F. Avaliação de marcadores SSR com potencial para seleção assistida de videiras apirências e resistentes ao míldio. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 10.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 6., 2012, Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2012. p. 12. Resumo.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 11. | | MALABARBA, J.; WAIRICH, A.; BUFFON, V.; CZERMAINSKI, A. B. C.; REVERS, L. F. Avaliação de marcadores SSR com potencial para seleção assistida de videiras apirênicas e resistentes ao míldio. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 22., 2012, Bento Gonçalves. Anais... Bento Gonçalves: SBF, p. 4401-4404, 2012.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 14. | | WAIRICH, A.; MALABARBA, J.; BUFFON, V.; PORTO, D. D.; REVERS, L. F. Perfil transcricional de genes candidatos associados à resistência ao P. viticola na cultivar resistente Villard Blanc e na cultivar suscetível Cabernet Sauvignon. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 10.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 6., 2012, Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2012. p. 11. Resumo.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 15. | | MALABARBA, J.; BUFFON, V.; WAIRICH, A.; GAETA, M. L.; MARIATH, J. E. A.; DORNELAS, M. C.; REVERS, L. F. Gene expression analysis of VVAG3 in grapevine reproductive tissues by in situ hybridization. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 4., 2013, Bento Gonçalves. Resumos... Bento Gonçalves: Sociedade Brasileira de Genética, 2013. p. 3. Resumo.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 16. | | MALABARBA, J.; BUFFON, V.; FRÂNCIO, L.; MARASCHIN, F. S.; PASQUALI, G.; MÁRGIS-PINHEIRO, M. N.; REVERS, L. F. Avaliação da função de VvAGL11 na morfogênese da semente da videira. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 14. ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 10., 2016, Bento Gonçalves. Resumos...Bento Gonçalves, RS: Embrapa uva e Vinho, 2016. p. 46. (Embrapa Uva e Vinho. Documentos, 99)| Biblioteca(s): Embrapa Uva e Vinho. |
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| 17. | | MALABARBA, J.; BUFFON, V.; MARIATH, J. E. A.; MARASHIN, F. S.; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. Manipulation of VviAGL11 expression changes the seed content in grapevine (Vitis vinifera L.). Plant Science, v. 269, p. 126-135, 2018.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 19. | | MALABARBA, J.; BUFFON, V.; MARIATH, J. E. A.; GAETA, M. L.; DORNELAS, M. C.; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. The MADS-box gene Agamous-like 11 is essential for seed morphogenesis in grapevine. Journal of Experimental Botany, v. 68, n. 7, p. 1493-1506, mar. 2017.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 20. | | MALABARBA, J.; BUFFON, V.; MARIATH, J. E. de A.; DORNELAS, M. C.; PINHEIRO-MARGIS, M. M. A. N.; PASQUALI, G.; REVERS, L. F. Molecular anatomy of seedlessness in grapevine: the role of VvAGL11 during seed morphogenesis. In: CONGRESSO LATINO-AMERICANO DE VITICULTURA E ENOLOGIA, 15.; CONGRESSO BRASILEIRO DE VITICULTURA E ENOLOGIA, 13., 2015, Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho: Associação Brasileira de Enologia, 2015. p. 389. XV Congresso Latino-Americano de Viticultura e Enologia E XIII Congresso Brasileiro de Viticultura e Enologia. Bento Gonçalves-RS, 3 a 7 de Novembro de 2015.| Biblioteca(s): Embrapa Uva e Vinho. |
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| Registros recuperados : 25 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido; Embrapa Uva e Vinho. |
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Data corrente: |
24/02/2022 |
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Data da última atualização: |
14/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
WAIRICH, A.; MALABARBA, J.; BUFFON, V.; PORTO, D. D.; TOGAWA, R. C.; REVERS, L. F. |
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Afiliação: |
ANDRIELE WAIRICH, Universidade Federal do Rio Grande do Sul (UFRGS); JAIANA MALABARBA, CNPUV; VANESSA BUFFON, CNPUV; DIOGO DENARDI PORTO, CPATSA; ROBERTO COITI TOGAWA, Cenargen; LUIS FERNANDO REVERS, CNPUV. |
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Título: |
Molecular characterization of the Rpv3 locus towards the development of KASP markers for downy mildew resistance in grapevine (Vitis spp.). |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Euphytica, v. 218, 2022. Article, 5. |
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DOI: |
https://doi.org/10.1007/s10681-021-02952-3 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Roberto Togawa. |
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Conteúdo: |
Plasmopara viticola is the oomycete that causes downy mildew in grapevines. Varying levels of resistance to P. viticola across grape cultivars allowed quantitative trait loci to be identified. The Rpv3 locus is located at chromosome 18, in a region enriched in TIR-NBS-LRR genes, and the phenotype associated is a high hypersensitive response. In this work, we aimed to identify candidate genes associated with resistance to downy mildew on the Rpv3 locus and to evaluate their transcriptional profiles in a susceptible and a resistant grapevine cultivar after challenging with P. viticola. Candidate genes were identified by in silico functional enrichment tests. Many predicted genes associated with resistance to diseases were found at the Rpv3 locus. In total, seventeen genes were evaluated by RT-qPCR. Differences in the steady-state expression of these genes were observed between the two cultivars. Four genes were found to be expressed only in Villard Blanc, suggesting their association to the hypersensitivity reaction. Aiming to assist marker assisted-selection for downy mildew resistance, we show the efficient use of a set of SSR markers. Furthermore, from on a set of forty-one Rpv3-located SNPs, whose segregation was tested in the populations studied, the two segregating markers, Rpv3_15 and Rpv3_33, were considered efficient for downy mildew resistance identification. This study presents a genomic characterization of the Rpv3 locus, confirms its involvement in resistance against P. viticola infection and presents promising biotechnological tools for the selection of young resistant individuals. MenosPlasmopara viticola is the oomycete that causes downy mildew in grapevines. Varying levels of resistance to P. viticola across grape cultivars allowed quantitative trait loci to be identified. The Rpv3 locus is located at chromosome 18, in a region enriched in TIR-NBS-LRR genes, and the phenotype associated is a high hypersensitive response. In this work, we aimed to identify candidate genes associated with resistance to downy mildew on the Rpv3 locus and to evaluate their transcriptional profiles in a susceptible and a resistant grapevine cultivar after challenging with P. viticola. Candidate genes were identified by in silico functional enrichment tests. Many predicted genes associated with resistance to diseases were found at the Rpv3 locus. In total, seventeen genes were evaluated by RT-qPCR. Differences in the steady-state expression of these genes were observed between the two cultivars. Four genes were found to be expressed only in Villard Blanc, suggesting their association to the hypersensitivity reaction. Aiming to assist marker assisted-selection for downy mildew resistance, we show the efficient use of a set of SSR markers. Furthermore, from on a set of forty-one Rpv3-located SNPs, whose segregation was tested in the populations studied, the two segregating markers, Rpv3_15 and Rpv3_33, were considered efficient for downy mildew resistance identification. This study presents a genomic characterization of the Rpv3 locus, confirms its involvement in resistance agai... Mostrar Tudo |
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Palavras-Chave: |
Downy mildew resistance; Resistência ao míldio; Resistência ao oídio; Rpv3 locus; RT-qPCR; V vinifera. |
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Thesagro: |
Doença; Míldio; Uva; Vitis Vinifera. |
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Thesaurus NAL: |
Grapes; Marker-assisted selection; Plasmopara viticola. |
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Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/232084/1/Wairich2021-Article-MolecularCharacterizationOfThe.pdf
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Marc: |
LEADER 02696naa a2200361 a 4500
001 2140494
005 2022-07-14
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s10681-021-02952-3$2DOI
100 1 $aWAIRICH, A.
245 $aMolecular characterization of the Rpv3 locus towards the development of KASP markers for downy mildew resistance in grapevine (Vitis spp.).$h[electronic resource]
260 $c2022
500 $aNa publicação: Roberto Togawa.
520 $aPlasmopara viticola is the oomycete that causes downy mildew in grapevines. Varying levels of resistance to P. viticola across grape cultivars allowed quantitative trait loci to be identified. The Rpv3 locus is located at chromosome 18, in a region enriched in TIR-NBS-LRR genes, and the phenotype associated is a high hypersensitive response. In this work, we aimed to identify candidate genes associated with resistance to downy mildew on the Rpv3 locus and to evaluate their transcriptional profiles in a susceptible and a resistant grapevine cultivar after challenging with P. viticola. Candidate genes were identified by in silico functional enrichment tests. Many predicted genes associated with resistance to diseases were found at the Rpv3 locus. In total, seventeen genes were evaluated by RT-qPCR. Differences in the steady-state expression of these genes were observed between the two cultivars. Four genes were found to be expressed only in Villard Blanc, suggesting their association to the hypersensitivity reaction. Aiming to assist marker assisted-selection for downy mildew resistance, we show the efficient use of a set of SSR markers. Furthermore, from on a set of forty-one Rpv3-located SNPs, whose segregation was tested in the populations studied, the two segregating markers, Rpv3_15 and Rpv3_33, were considered efficient for downy mildew resistance identification. This study presents a genomic characterization of the Rpv3 locus, confirms its involvement in resistance against P. viticola infection and presents promising biotechnological tools for the selection of young resistant individuals.
650 $aGrapes
650 $aMarker-assisted selection
650 $aPlasmopara viticola
650 $aDoença
650 $aMíldio
650 $aUva
650 $aVitis Vinifera
653 $aDowny mildew resistance
653 $aResistência ao míldio
653 $aResistência ao oídio
653 $aRpv3 locus
653 $aRT-qPCR
653 $aV vinifera
700 1 $aMALABARBA, J.
700 1 $aBUFFON, V.
700 1 $aPORTO, D. D.
700 1 $aTOGAWA, R. C.
700 1 $aREVERS, L. F.
773 $tEuphytica$gv. 218, 2022. Article, 5.
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Embrapa Uva e Vinho (CNPUV) |
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