Data/hora: 24/09/2024 07:14:33

Biblioteca(s): 	Embrapa Semiárido.	
Data corrente: 	24/02/2022	
Data da última atualização: 	01/03/2023	
Autoria: 	MAKTURA, G. C.; PARANHOS, B. A. J.; SOUZA, H. M.	
Afiliação: 	GRAZIELLE C. MAKTURA; BEATRIZ AGUIAR GIORDANO PARANHOS, CPATSA; HENRIQUE MARQUES SOUZA.	
Título: 	RNAi in fruit flies (Diptera: Tephritidae): successes and challenges.	
Ano de publicação: 	2021	
Fonte/Imprenta: 	Journal of Applied Entomology, v. 145, n. 8, p. 740-756, 2021.	
DOI: 	10.1111/jen.12905	
Idioma: 	Inglês	
Conteúdo: 	Fruit flies (Diptera: Tephritidae) are among the most economically important pests worldwide, causing direct damage to fruit crops through female oviposition and lar-val feeding. Currently, the most widely disseminated methods of control of fruit flies involve the adoption of cultural and biological techniques, like the sterile insect tech-nique, and the use of agrochemicals. However, the latter depend on the concomitant use of other techniques, especially chemical ones, which are the most successful, but also related to resistance, off-target effects and pollution, showing the need for new methods. RNA interference (RNAi) is a biotechnological tool that is capable of in-ducing post-transcriptional gene silencing using double-stranded RNA (dsRNA). The mechanism could be exploited in pest control associated with integrated pest man-agement, by targeting essential genes or by increasing the susceptibility of insects to insecticides. Here, we discuss the RNAi machinery and the uptake genes of the main Tephritidae species and their differences in comparison with other insects. Then, we investigate the international literature on the application of this tool to improve the sterile insect technique, enhance the insects? susceptibility to insecticides and modu-late their growth and development, demonstrating the sensibility of Tephritidae spe-cies to RNAi. Finally, we discuss the main challenges to the future use of RNAi as a method to control Tephritidae, such as delivery procedures, degradation and produc-tion of dsRNA, off-target effects, occurrence of refractoriness, and perspectives and shortages associated with its adoption that need to be considered.	
Palavras-Chave: 	Inseto estéril; Interferência de RNA; Manejo integrado de pragas; RNAi; Silenciamento de genes; Técnica de inseto estéril; Tephritida.	
Thesagro: 	Controle Biológico; Mosca das Frutas.	
Thesaurus Nal: 	Biological control; Fruit flies.	
Categoria do assunto: 	O Insetos e Entomologia	
Registro original: 	Embrapa Semiárido (CPATSA)	
Exemplares: 	CPATSA - (59799-1) - UPC - AP - DD -  -  -  - DIS