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 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
13/05/2020 |
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Data da última atualização: |
05/01/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TAHERI, S.; DECAËNS, T.; CUNHA, L.; BROWN, G. G.; SILVA, E. da; BARTZ, M. L. C.; BARETTA, D.; DUPONT, L. |
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Afiliação: |
Sorbonne Universite; Universite de Montpellier; University of South Wales; GEORGE GARDNER BROWN, CNPF; Universidade Positivo; Universidade do Estado de Santa Catarina; Universite Paris. |
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Título: |
Genetic evidence of multiple introductions and mixed reproductive strategy in the peregrine earthworm Pontoscolex corethrurus. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Biological Invasions, v. 22, p. 2545-2557, 2020. |
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DOI: |
DOI 10.1007/s10530-020-02270-0 |
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Idioma: |
Inglês |
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Conteúdo: |
Pontoscolex corethrurus is a well-known invasive earthworm in tropical zone which is believed to have originated from the Guayana Shield in South America and was described as parthenogenetic. A recent phylogenetic study revealed four cryptic species in the P. corethrurus complex (L1, L2, L3 and L4), among them L1 was particularly widespread and was proposed as P. corethrurus sensu stricto. Here, our aims were to investigate the genetic variation of P. corethrurus L1 in its presumed native and introduced ranges and to examine its reproductive strategy. An extensive dataset of 478 cytochrome oxidase I gene (COI) sequences, obtained in specimens sampled all around the world, revealed a weak COI haplotype diversity with one major haplotype (H1) present in 76% of the specimens. Analyses of the genetic variation of 12 L1 populations were done using both nuclear (226 AFLP profiles) and mitochondrial (269 COI sequences) genetic information. The high AFLP genotype diversity at the worldwide scale and the fact that no genotype was shared among populations, allowed to reject the ?super-clone? invasion hypothesis. Moreover, a similar level of mean genetic diversity indices were observed between the introduced and native ranges, a pattern explained by a history of multiple introductions of specimens from different parts of the world. At last, occurrence of identical AFLPs genotypes (i.e. clones) in several population confirmed asexual reproduction, but recombination was also revealed by gametic equilibrium analysis in some populations suggesting that P. corethrurus L1 may have a mixed reproductive strategy. MenosPontoscolex corethrurus is a well-known invasive earthworm in tropical zone which is believed to have originated from the Guayana Shield in South America and was described as parthenogenetic. A recent phylogenetic study revealed four cryptic species in the P. corethrurus complex (L1, L2, L3 and L4), among them L1 was particularly widespread and was proposed as P. corethrurus sensu stricto. Here, our aims were to investigate the genetic variation of P. corethrurus L1 in its presumed native and introduced ranges and to examine its reproductive strategy. An extensive dataset of 478 cytochrome oxidase I gene (COI) sequences, obtained in specimens sampled all around the world, revealed a weak COI haplotype diversity with one major haplotype (H1) present in 76% of the specimens. Analyses of the genetic variation of 12 L1 populations were done using both nuclear (226 AFLP profiles) and mitochondrial (269 COI sequences) genetic information. The high AFLP genotype diversity at the worldwide scale and the fact that no genotype was shared among populations, allowed to reject the ?super-clone? invasion hypothesis. Moreover, a similar level of mean genetic diversity indices were observed between the introduced and native ranges, a pattern explained by a history of multiple introductions of specimens from different parts of the world. At last, occurrence of identical AFLPs genotypes (i.e. clones) in several population confirmed asexual reproduction, but recombination was also revealed by ... Mostrar Tudo |
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Palavras-Chave: |
Genetic diversity; Soil macrofauna; Tropical invasive species. |
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Thesaurus Nal: |
Founder effect; Genetic recombination; Parthenogenesis. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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Marc: |
LEADER 02500naa a2200289 a 4500
001 2122302
005 2021-01-05
008 2020 bl uuuu u00u1 u #d
024 7 $aDOI 10.1007/s10530-020-02270-0$2DOI
100 1 $aTAHERI, S.
245 $aGenetic evidence of multiple introductions and mixed reproductive strategy in the peregrine earthworm Pontoscolex corethrurus.$h[electronic resource]
260 $c2020
520 $aPontoscolex corethrurus is a well-known invasive earthworm in tropical zone which is believed to have originated from the Guayana Shield in South America and was described as parthenogenetic. A recent phylogenetic study revealed four cryptic species in the P. corethrurus complex (L1, L2, L3 and L4), among them L1 was particularly widespread and was proposed as P. corethrurus sensu stricto. Here, our aims were to investigate the genetic variation of P. corethrurus L1 in its presumed native and introduced ranges and to examine its reproductive strategy. An extensive dataset of 478 cytochrome oxidase I gene (COI) sequences, obtained in specimens sampled all around the world, revealed a weak COI haplotype diversity with one major haplotype (H1) present in 76% of the specimens. Analyses of the genetic variation of 12 L1 populations were done using both nuclear (226 AFLP profiles) and mitochondrial (269 COI sequences) genetic information. The high AFLP genotype diversity at the worldwide scale and the fact that no genotype was shared among populations, allowed to reject the ?super-clone? invasion hypothesis. Moreover, a similar level of mean genetic diversity indices were observed between the introduced and native ranges, a pattern explained by a history of multiple introductions of specimens from different parts of the world. At last, occurrence of identical AFLPs genotypes (i.e. clones) in several population confirmed asexual reproduction, but recombination was also revealed by gametic equilibrium analysis in some populations suggesting that P. corethrurus L1 may have a mixed reproductive strategy.
650 $aFounder effect
650 $aGenetic recombination
650 $aParthenogenesis
653 $aGenetic diversity
653 $aSoil macrofauna
653 $aTropical invasive species
700 1 $aDECAËNS, T.
700 1 $aCUNHA, L.
700 1 $aBROWN, G. G.
700 1 $aSILVA, E. da
700 1 $aBARTZ, M. L. C.
700 1 $aBARETTA, D.
700 1 $aDUPONT, L.
773 $tBiological Invasions$gv. 22, p. 2545-2557, 2020.
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Registro original: |
Embrapa Florestas (CNPF) |
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Biblioteca |
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Registro |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/06/2011 |
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Data da última atualização: |
14/02/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - C |
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Autoria: |
PACHECO, A. C. L.; OLIVEIRA, S. M. P.; GOUVEIA, J. J. S.; DINIZ, M. C.; VASCONCELOS, E. J. R.; VIANA, D. de A.; Gracia Maria Soares ROSINHA, G. M. S.; MAGGIONI, R. |
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Afiliação: |
Ana Carolina Landim Pacheco, Universidade Federal do Ceará (UFC), Fortaleza, CE.; Sonia Maria Pinheiro OLIVEIRA, UFC, Fortaleza, CE.; João José Simoni Gouveia, 2NUGEN, Núcleo de Genômica e Bioinformática/Faculdade de Veterinária/ Universidade Estadual do Ceará (UECe); Michely Correia Diniz, 2NUGEN, Núcleo de Genômica e Bioinformática/Faculdade de Veterinária/ Universidade Estadual do Ceará; Elton José Rosas Vasconcelos, 2NUGEN, Núcleo de Genômica e Bioinformática/Faculdade de Veterinária/ Universidade Estadual do Ceará; Daniel de Araújo VIANA, 2NUGEN, Núcleo de Genômica e Bioinformática/Faculdade de Veterinária/ Universidade Estadual do Ceará; Gracia Maria Soares Rosinha, CNPC; Rodrigo MAGGIONI, NUGEN; Universidade Estadual do Ceará. |
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Título: |
Analysis of prion protein gene (prnp) polymorphisms in healthy Morada Nova sheep reveals the presence of genotypes susceptible to scrapie. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Ciência Animal, Fortaleza, v. 17, n. 1, p. 27-36, 2007. |
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Idioma: |
Inglês |
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Conteúdo: |
Prion diseases, such as sheep scrapie, are usually associated with certain genotypes of prion protein gene (PRNP). Polymorphisms at codons 136, 154 and 171 of ovine PRNP open reading frame (ORF) are believed to confer either resistance or susceptibility to scrapie. In this study 72 healthy sheep from two different flocks, representing two meat type color variants from hair breed Morada Nova, from the State of Ceará, Brazil, were analyzed. PRNP ORF sequences were investigated for single nucleotide polymorphisms (SNPs) detection, followed by genotype analysis of codons 136, 154 and 171. Well known polymorphisms at codons 136 (coding for A/V) and 171 (coding for Q/R) were identified among the subjects, while at codon 154 only codon R has been observed. PRNP genotypes observed among Morada Nova subjects were ARQ/ ARQ (34.75%), ARQ/ARR (30.49%), ARR/ARR (31.92%) and the rare VRR/VRR (2.78%). We suggest here that the observed high homozygote frequency among Morada Nova PRNP genotypes AA at codon 136 and RR at codon 171 could be a genetic element for a putative natural resistance to scrapie. This is the first report of PRNP genotyping in Morada Nova breed and the first time the scrapie susceptible allele VRR has been identified in Brazil. RESUMO - Doenças priônicas, como o scrapie (Paraplexia Enzoótica dos Ovinos) estão usualmente associadas com determinados genótipos do gene da proteína priônica (PRNP). Polimorfismos na matriz aberta de leitura (ORF) dos códons 136, 154 e 171 de PRNP de ovinos estão associados a resistência ou suscetibilidade a scrapie. Neste estudo, um total de 72 ovinos saudáveis, de 2 rebanhos e diferente variação de cor de pelagem, da raça deslanada Morada Nova no Estado do Ceará, foram analisadas. Seqüências PRNP, compreendendo as ORF, do gene PRNP foram investigadas para a identificação dos Polimorfismos de único nucleotídeo (SNPs), seguida de detalhado seqüenciamento do DNA e análise bioinformática para os códons 136, 154 e 171. As freqüências genotípicas dos polimorfismos do PRNP são relatadas. Os polimorfismos já identificados no códon 136 (codificando A/V), 171 (codificando Q/R), entretanto no códon 154 não houve variação (somente R). As variantes alélicas e genotípicas para o PRNP observadas entre os ovinos Morada Nova foram: ARQ/ARQ (34,75%), ARQ/ARR (30,49%), ARR/ARR (31,92%) além do genótipo raro VRR/VRR (2,78%). Além das variantes alélicas e genótipicas, nós discutimos a freqüência da homozigozidade dos alelos AA no códon 136 e RR no códon 171 do PRNP e correspondentes haplótipos nos ovinos Morada Nova como elementos genéticos como um suposto elemento de resistência natural a scrapie. Este é o primeiro relato de genotipagem para PRNP em ovinos da raça Morada Nova e primeira observação do genótipo VRR/VRR em rebanho brasileiro. MenosPrion diseases, such as sheep scrapie, are usually associated with certain genotypes of prion protein gene (PRNP). Polymorphisms at codons 136, 154 and 171 of ovine PRNP open reading frame (ORF) are believed to confer either resistance or susceptibility to scrapie. In this study 72 healthy sheep from two different flocks, representing two meat type color variants from hair breed Morada Nova, from the State of Ceará, Brazil, were analyzed. PRNP ORF sequences were investigated for single nucleotide polymorphisms (SNPs) detection, followed by genotype analysis of codons 136, 154 and 171. Well known polymorphisms at codons 136 (coding for A/V) and 171 (coding for Q/R) were identified among the subjects, while at codon 154 only codon R has been observed. PRNP genotypes observed among Morada Nova subjects were ARQ/ ARQ (34.75%), ARQ/ARR (30.49%), ARR/ARR (31.92%) and the rare VRR/VRR (2.78%). We suggest here that the observed high homozygote frequency among Morada Nova PRNP genotypes AA at codon 136 and RR at codon 171 could be a genetic element for a putative natural resistance to scrapie. This is the first report of PRNP genotyping in Morada Nova breed and the first time the scrapie susceptible allele VRR has been identified in Brazil. RESUMO - Doenças priônicas, como o scrapie (Paraplexia Enzoótica dos Ovinos) estão usualmente associadas com determinados genótipos do gene da proteína priônica (PRNP). Polimorfismos na matriz aberta de leitura (ORF) dos códons 136, 154 e 171 de P... Mostrar Tudo |
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Palavras-Chave: |
x. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/35708/1/API-Analysis-of-prion.pdf
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Marc: |
LEADER 03572naa a2200217 a 4500
001 1890225
005 2013-02-14
008 2007 bl uuuu u00u1 u #d
100 1 $aPACHECO, A. C. L.
245 $aAnalysis of prion protein gene (prnp) polymorphisms in healthy Morada Nova sheep reveals the presence of genotypes susceptible to scrapie.$h[electronic resource]
260 $c2007
520 $aPrion diseases, such as sheep scrapie, are usually associated with certain genotypes of prion protein gene (PRNP). Polymorphisms at codons 136, 154 and 171 of ovine PRNP open reading frame (ORF) are believed to confer either resistance or susceptibility to scrapie. In this study 72 healthy sheep from two different flocks, representing two meat type color variants from hair breed Morada Nova, from the State of Ceará, Brazil, were analyzed. PRNP ORF sequences were investigated for single nucleotide polymorphisms (SNPs) detection, followed by genotype analysis of codons 136, 154 and 171. Well known polymorphisms at codons 136 (coding for A/V) and 171 (coding for Q/R) were identified among the subjects, while at codon 154 only codon R has been observed. PRNP genotypes observed among Morada Nova subjects were ARQ/ ARQ (34.75%), ARQ/ARR (30.49%), ARR/ARR (31.92%) and the rare VRR/VRR (2.78%). We suggest here that the observed high homozygote frequency among Morada Nova PRNP genotypes AA at codon 136 and RR at codon 171 could be a genetic element for a putative natural resistance to scrapie. This is the first report of PRNP genotyping in Morada Nova breed and the first time the scrapie susceptible allele VRR has been identified in Brazil. RESUMO - Doenças priônicas, como o scrapie (Paraplexia Enzoótica dos Ovinos) estão usualmente associadas com determinados genótipos do gene da proteína priônica (PRNP). Polimorfismos na matriz aberta de leitura (ORF) dos códons 136, 154 e 171 de PRNP de ovinos estão associados a resistência ou suscetibilidade a scrapie. Neste estudo, um total de 72 ovinos saudáveis, de 2 rebanhos e diferente variação de cor de pelagem, da raça deslanada Morada Nova no Estado do Ceará, foram analisadas. Seqüências PRNP, compreendendo as ORF, do gene PRNP foram investigadas para a identificação dos Polimorfismos de único nucleotídeo (SNPs), seguida de detalhado seqüenciamento do DNA e análise bioinformática para os códons 136, 154 e 171. As freqüências genotípicas dos polimorfismos do PRNP são relatadas. Os polimorfismos já identificados no códon 136 (codificando A/V), 171 (codificando Q/R), entretanto no códon 154 não houve variação (somente R). As variantes alélicas e genotípicas para o PRNP observadas entre os ovinos Morada Nova foram: ARQ/ARQ (34,75%), ARQ/ARR (30,49%), ARR/ARR (31,92%) além do genótipo raro VRR/VRR (2,78%). Além das variantes alélicas e genótipicas, nós discutimos a freqüência da homozigozidade dos alelos AA no códon 136 e RR no códon 171 do PRNP e correspondentes haplótipos nos ovinos Morada Nova como elementos genéticos como um suposto elemento de resistência natural a scrapie. Este é o primeiro relato de genotipagem para PRNP em ovinos da raça Morada Nova e primeira observação do genótipo VRR/VRR em rebanho brasileiro.
653 $ax
700 1 $aOLIVEIRA, S. M. P.
700 1 $aGOUVEIA, J. J. S.
700 1 $aDINIZ, M. C.
700 1 $aVASCONCELOS, E. J. R.
700 1 $aVIANA, D. de A.
700 1 $aGracia Maria Soares ROSINHA, G. M. S.
700 1 $aMAGGIONI, R.
773 $tCiência Animal, Fortaleza$gv. 17, n. 1, p. 27-36, 2007.
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