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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
11/03/2014 |
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Data da última atualização: |
12/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TIZIOTO, P.; GASPARINI, G.; SOUZA, M. M.; MUDADU, M. de A.; COUTINHO, L. L.; MOURÃO, G. B.; THOLON, P.; MEIRELLES, S. L. C.; TULLIO, R. R.; ROSA, A. do N.; ALENCAR, M. M. de; MEDEIROS, S. R.; SIQUEIRA, F.; FEIJO, G. L. D.; NASSU, R. T.; REGITANO, L. C. de A. |
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Afiliação: |
POLIANA TIZIOTO, Universidade Federal de São Carlos; GUSTAVO GASPARINI, POS-GRADUAÇÃO UFSCar/SÃO CARLOS, SP; MARCELA M. SOUZA, POS-GRADUAÇÃO UFSCar/SÃO CARLOS, SP; MAURICIO DE ALVARENGA MUDADU, CPPSE; LUIZ L. COUTINHO, PROF. ESALQ/PIRACICABA; GERSON B. MOURÃO, ESALQ/PIRACICABA; PATRICIA THOLON, CPPSE; SARAH L. C. MEIRELLES, PROF. UFLA/LAVRAS; RYMER RAMIZ TULLIO, CPPSE; ANTONIO DO NASCIMENTO ROSA, CNPGC; MAURICIO MELLO DE ALENCAR, CPPSE; SERGIO R. MEDEIROS, CAMPO GRANDE; FABIANE SIQUEIRA, CNPGC; GELSON LUIS DIAS FEIJO, CNPGC; RENATA TIEKO NASSU, CPPSE; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
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Título: |
Identification of KCNJ11 as a functional candidate gene for bovine meat tenderness. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Physiological Genomics, v. 45, n. 24, p. 1215-1221, 2013. |
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DOI: |
10.1152/physiolgenomics.00137.2012 |
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Idioma: |
Inglês |
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Conteúdo: |
The potassium inwardly rectifying channel, subfamily J, member 11 (KCNJ11) gene was investigated as a candidate for meat tenderness based on the effects reported on muscle for KCNJ11 gene knockout in rat models and its position in a quantitative trait locus (QTL) for meat tenderness in the bovine genome. Sequence variations in the KCNJ11 gene were described by sequencing six amplified fragments, covering almost the entire gene. We identified single nucleotide polymorphisms (SNP) and validated them by different approaches, taking advantage of simultaneous projects that are being developed with the same Nelore population. By sequencing the KCNJ11 in Nelore steers representing extreme phenotypes for Warner-Bratzler shear force (WBSF), it was possible to identify 22 SNPs. We validated two of the identified markers by genotyping the whole population (n = 460). Analysis of association between genotypes and WBSF values revealed a significant additive effect of a SNP at different meat aging times (P ? 0.05). In addition, an association between the expression levels of KCNJ11 and WBSF was found, with lower expression levels of KCNJ11 associated with more tender meat (P ? 0.05). The results showed that the KCNJ11 gene is a candidate mapped to a QTL for meat tenderness previously identified on BTA15 and may be useful to identify animals with genetic potential to produce tender meat. The effect of KCNJ11 observed on muscle is potentially due to changes in activity of KATP channels, which in turn influence the flow of potassium in the intracellular space, allowing establishment of the membrane potential necessary for muscle contraction. MenosThe potassium inwardly rectifying channel, subfamily J, member 11 (KCNJ11) gene was investigated as a candidate for meat tenderness based on the effects reported on muscle for KCNJ11 gene knockout in rat models and its position in a quantitative trait locus (QTL) for meat tenderness in the bovine genome. Sequence variations in the KCNJ11 gene were described by sequencing six amplified fragments, covering almost the entire gene. We identified single nucleotide polymorphisms (SNP) and validated them by different approaches, taking advantage of simultaneous projects that are being developed with the same Nelore population. By sequencing the KCNJ11 in Nelore steers representing extreme phenotypes for Warner-Bratzler shear force (WBSF), it was possible to identify 22 SNPs. We validated two of the identified markers by genotyping the whole population (n = 460). Analysis of association between genotypes and WBSF values revealed a significant additive effect of a SNP at different meat aging times (P ? 0.05). In addition, an association between the expression levels of KCNJ11 and WBSF was found, with lower expression levels of KCNJ11 associated with more tender meat (P ? 0.05). The results showed that the KCNJ11 gene is a candidate mapped to a QTL for meat tenderness previously identified on BTA15 and may be useful to identify animals with genetic potential to produce tender meat. The effect of KCNJ11 observed on muscle is potentially due to changes in activity of KATP channels, whic... Mostrar Tudo |
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Palavras-Chave: |
Bovine; Candidate gene; Differential expression; Molecular markers; Shear force; Warner Bratzler. |
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Thesaurus Nal: |
beef; meat. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/108204/1/1215.full.pdf
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Marc: |
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024 7 $a10.1152/physiolgenomics.00137.2012$2DOI
100 1 $aTIZIOTO, P.
245 $aIdentification of KCNJ11 as a functional candidate gene for bovine meat tenderness.$h[electronic resource]
260 $c2013
520 $aThe potassium inwardly rectifying channel, subfamily J, member 11 (KCNJ11) gene was investigated as a candidate for meat tenderness based on the effects reported on muscle for KCNJ11 gene knockout in rat models and its position in a quantitative trait locus (QTL) for meat tenderness in the bovine genome. Sequence variations in the KCNJ11 gene were described by sequencing six amplified fragments, covering almost the entire gene. We identified single nucleotide polymorphisms (SNP) and validated them by different approaches, taking advantage of simultaneous projects that are being developed with the same Nelore population. By sequencing the KCNJ11 in Nelore steers representing extreme phenotypes for Warner-Bratzler shear force (WBSF), it was possible to identify 22 SNPs. We validated two of the identified markers by genotyping the whole population (n = 460). Analysis of association between genotypes and WBSF values revealed a significant additive effect of a SNP at different meat aging times (P ? 0.05). In addition, an association between the expression levels of KCNJ11 and WBSF was found, with lower expression levels of KCNJ11 associated with more tender meat (P ? 0.05). The results showed that the KCNJ11 gene is a candidate mapped to a QTL for meat tenderness previously identified on BTA15 and may be useful to identify animals with genetic potential to produce tender meat. The effect of KCNJ11 observed on muscle is potentially due to changes in activity of KATP channels, which in turn influence the flow of potassium in the intracellular space, allowing establishment of the membrane potential necessary for muscle contraction.
650 $abeef
650 $ameat
653 $aBovine
653 $aCandidate gene
653 $aDifferential expression
653 $aMolecular markers
653 $aShear force
653 $aWarner Bratzler
700 1 $aGASPARINI, G.
700 1 $aSOUZA, M. M.
700 1 $aMUDADU, M. de A.
700 1 $aCOUTINHO, L. L.
700 1 $aMOURÃO, G. B.
700 1 $aTHOLON, P.
700 1 $aMEIRELLES, S. L. C.
700 1 $aTULLIO, R. R.
700 1 $aROSA, A. do N.
700 1 $aALENCAR, M. M. de
700 1 $aMEDEIROS, S. R.
700 1 $aSIQUEIRA, F.
700 1 $aFEIJO, G. L. D.
700 1 $aNASSU, R. T.
700 1 $aREGITANO, L. C. de A.
773 $tPhysiological Genomics$gv. 45, n. 24, p. 1215-1221, 2013.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| 8. |  | NASCIMENTO, W. M.; GIORDANO, L. B.; BEVITORI, R.; MACHADO, N. F. Avaliação de cultivares de lentilha. Horticultura Brasileira, Brasilia, DF, v. 12, n. 1, p. 93, maio 1994. Ref. 155. Edição dos Resumos do XXXIV Congresso Brasileiro de Olericultura, Águas de São Pedro, SP, 1994.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 16. | | COUTINHO, E, F.; CARLLOTO, F.; CAPELLARO, T, H.; TESSMER, M, A; MACHADO, N, P. Propagação vegetativa de pessegueiro `eragil´ por alporquia. In: ENCONTRO NACIONAL SOBRE FRUTICULTURA DE CLIMA TEMPERADO, 10., 2007, Fraiburgo. Resumos... Caçador: EPAGRI, Estação Experimental de Caçador, 2007. v. 2 p. 21| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 17. | | GOMES, F. R. C.; COUTINHO, E. F.; GOMES, G. C.; MACHADO, N. P.; NOREMBERG, E. M. Quintais orgânicos de frutas: contribuição para a segurança alimentar em áreas rurais, indígenas e urbanas. CONGRESSO BRASILEIRO DE AGROECOLOGIA, 2.; SEMINÁRIO INTERNACIONAL SOBRE AGROECOLOGIA, 5.; SEMINÁRIO ESTADUAL SOBRE AGROECOLOGIA, 6., 2004, Porto Alegre. Agrobiodiversidade: base para sociedades sustentáveis: anais... Porto Alegre: EMATER - RS, 2004. 1 CD-ROM Trabalhos apresentados no CONGRESSO BRASILEIRO DE AGROECOLOGIA, 2.; SEMINÁRIO INTERNACIONAL SOBRE AGROECOLOGIA, 5.; SEMINÁRIO ESTADUAL SOBRE AGROECOLOGIA, 6., 2004, Porto Alegre. Agrobiodiversidade: base para sociedades sustentáveis:...| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: Nacional - C |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 18. | | FINKENAUER, D.; COMIOTTO, A.; MOURA, G. C.; MACHADO, N. P.; ANTUNES, L. E. C. Influência do horário de colheita nas condições físico-químicas de framboesas. In: CONGRESSO DE INICIAÇÃO CIENTIFICA, 18.; ENCONTRO DE PÓS GRADUAÇÃO, 11., E MOSTRA CIENTIFICA, 1., 2009, Pelotas. Anais... Pelotas: UFPel, 2009. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 19. | | COMIOTTO, A.; FACHINELLO, J. C.; HOFFMANN, A.; MACHADO, N. P.; GALARÇA, S. P.; BETEMPS, D. L. Vigor, floração, produção e qualidade de pêssegos 'Chimarrita' e 'Maciel' em função de diferentes porta-enxertos. Vigour, flowering, yield and quality of 'Chimarita' and 'Maciel' for different rootstocks. Ciência Rural, Santa Maria, v. 42, n. 5, p. 788-794, maio 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 20. | | EID, J. G.; DIAS, C. S.; LOURENÇO, R. M.; CORRÊA, O. A.; MOTA, S. A. P. da; MACHADO, N. T. Estudo do processo de destilação do diesel vegetal de palma (Elaeis guineensis, Jacq.) em escala de bancada, obtido através do processo de craqueamento, utilizando-se carbonato de sódio (Na2CO3) não regenerado como catalizador. In: CONGRESSO BRASILEIRO DE MAMONA, 5.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 2.; FÓRUM CAPIXABA DE PINHÃO-MANSO, 1., 2012, Guarapari. Desafios e Oportunidades: anais. Campina Grande: Embrapa Algodão, 2012. p. 5| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
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| Registros recuperados : 39 | |
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