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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
21/07/2022 |
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Data da última atualização: |
21/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GONÇALVES, M. S.; DORNELES, E. M. S.; HEINEMANN, M. B.; BRITO, M. A. V. P. e; GUIMARÃES, A. S. |
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Afiliação: |
MAYSA SERPA GONÇALVES, Universidade Federal de Lavras; ELAINE MARIA SELES DORNELES, Universidade Federal de Lavras; MARCOS BRYAN HEINEMANN, Universidade de São Paulo; MARIA APARECIDA VASCONCELOS PAIVA E BRITO; ALESSANDRO DE SA GUIMARAES, CNPGL. |
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Título: |
Genetic diversity and antimicrobial susceptibility of Staphylococcus aureus isolated from bovine mastitis in Minas Gerais, Brazil. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Ciência Rural, Santa Maria, v. 53, n. 3, e20210643, 2023. |
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DOI: |
https://doi.org/10.1590/0103-8478cr20210643 |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT - This study evaluated the genetic diversity and antimicrobial susceptibility of Staphylococcus aureus isolates from dairy cows in Minas Gerais, Brazil. Thirty-seven isolates from five municipalities (8 herds) were genotyped using multi-locus sequence typing (MLST) and susceptibility to 12 antimicrobial agents was tested using the disk diffusion method. High resistance rates for penicillin [75.68% (28/37)], ampicillin [70.27% (26/37)], and tetracycline [70.27% (26/37)] were detected. Multidrug resistance was observed in seven [18.92% (7/37)] isolates, and two were suggestive of Methicillin-resistant Staphylococcus aureus (MRSA). Among the 37 isolates, 33 novel sequence types (ST) and two known STs (ST126 and ST746) were identified in MLST. The clonal complexes more frequently observed were: CC97 [78.38%; (29/37)], CC1 [8.11%; (3/37)] and CC5 [5.40%; (2/37)]. Minimum?spanning tree (MST) analysis according to data from municipalities, herds, and resistance patterns for all isolates did not show any clustering pattern. However, the MST comparing all Brazilian S. aureus isolates deposited in the PubMLST database and from this study depicted an association between the genotype and strain origin (clinical sample). Isolates from this study that belong to CC97 were close to database isolates from milk and dairy products, while those that belong to CC1 and CC5 were close to database isolates from human sources and the environment of dairy farms or industries. In conclusion, our results showed a high rate of resistance to penicillins and tetracyclines and great genetic diversity among the S. aureus isolates from bovine mastitis genotyped in the present study. RESUMO - Este estudo teve como objetivo avaliar a diversidade genética e a suscetibilidade a antimicrobianos de cepas de Staphylococcus aureus isoladas de vacas leiteiras em Minas Gerais, Brasil. Trinta e sete cepas provenientes de cinco municípios (oito rebanhos) foram genotipadas usando a técnica multi-locus sequence typing (MLST) e a suscetibilidade a 12 antimicrobianos foi avaliada pelo método de difusão em disco. Foram detectadas altas taxas de resistência para penicilina [75,68% (28/37)], ampicilina [70,27% (26/37)] e tetraciclina [70,27% (26/37)] entre os isolados. A multirresistência foi observada em sete [18,92% (7/37)] isolados e dois foram classificados como sugestivos de Staphylococcus aureus resistente à meticilina (MRSA). Entre os 37 isolados, 33 novos sequence types (ST) e dois STs conhecidos (ST126 e ST746) foram identificados pelo MLST. Os complexos clonais mais frequentemente observados foram: CC97 [78,38%; (29/37)], CC1 [8,11%; (3/37)] e CC5 [5,40%; (2/37)]. Foi construída uma minimum?spanning tree (MSTs) com todos isolados estudados e esta não mostrou padrão de agrupamento quando comparada com dados epidemiológicos como municípios e rebanho, do qual foi isolado, e perfis de resistência a antimicrobianos. Uma segunda MST foi construída comparando os isolados deste estudo e todas as cepas de S. aureus depositadas no banco de dados PubMLST provenientes do Brasil, que mostrou associação entre o genótipo, STs e a origem da cepa. Foi possível observar entre os isolados do estudo que, aqueles que pertenciam ao CC97, eram geneticamente mais próximos das cepas depositadas no PubMLST isoladas de leite e de produtos lácteos, enquanto aqueles que pertenciam aos CC1 e CC5 estavam mais próximos a cepas isoladas de humanos ou do ambiente de fazendas e indústrias de laticínios. Em conclusão, nossos resultados mostraram um alto índice de resistência às penicilinas e tetraciclinas e grande diversidade genética entre as cepas de S. aureus isoladas de casos de mastite bovina. MenosABSTRACT - This study evaluated the genetic diversity and antimicrobial susceptibility of Staphylococcus aureus isolates from dairy cows in Minas Gerais, Brazil. Thirty-seven isolates from five municipalities (8 herds) were genotyped using multi-locus sequence typing (MLST) and susceptibility to 12 antimicrobial agents was tested using the disk diffusion method. High resistance rates for penicillin [75.68% (28/37)], ampicillin [70.27% (26/37)], and tetracycline [70.27% (26/37)] were detected. Multidrug resistance was observed in seven [18.92% (7/37)] isolates, and two were suggestive of Methicillin-resistant Staphylococcus aureus (MRSA). Among the 37 isolates, 33 novel sequence types (ST) and two known STs (ST126 and ST746) were identified in MLST. The clonal complexes more frequently observed were: CC97 [78.38%; (29/37)], CC1 [8.11%; (3/37)] and CC5 [5.40%; (2/37)]. Minimum?spanning tree (MST) analysis according to data from municipalities, herds, and resistance patterns for all isolates did not show any clustering pattern. However, the MST comparing all Brazilian S. aureus isolates deposited in the PubMLST database and from this study depicted an association between the genotype and strain origin (clinical sample). Isolates from this study that belong to CC97 were close to database isolates from milk and dairy products, while those that belong to CC1 and CC5 were close to database isolates from human sources and the environment of dairy farms or industries. In conclusion, ... Mostrar Tudo |
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Palavras-Chave: |
Mastite; MLST; Multidrug resistance; Multirresistência; Setor lácteo; Staphylococci; Zoonosis. |
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Thesagro: |
Bovino; Doença Animal; Estafilococo; Gado Leiteiro; Vaca Leiteira; Zoonose. |
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Thesaurus Nal: |
Dairy industry. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1144836/1/Genetic-diversity-and-antimicrobial-susceptibility-of-Staphylococcus-aureus.pdf
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Marc: |
LEADER 04769naa a2200349 a 4500
001 2144836
005 2022-07-21
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/0103-8478cr20210643$2DOI
100 1 $aGONÇALVES, M. S.
245 $aGenetic diversity and antimicrobial susceptibility of Staphylococcus aureus isolated from bovine mastitis in Minas Gerais, Brazil.$h[electronic resource]
260 $c2023
520 $aABSTRACT - This study evaluated the genetic diversity and antimicrobial susceptibility of Staphylococcus aureus isolates from dairy cows in Minas Gerais, Brazil. Thirty-seven isolates from five municipalities (8 herds) were genotyped using multi-locus sequence typing (MLST) and susceptibility to 12 antimicrobial agents was tested using the disk diffusion method. High resistance rates for penicillin [75.68% (28/37)], ampicillin [70.27% (26/37)], and tetracycline [70.27% (26/37)] were detected. Multidrug resistance was observed in seven [18.92% (7/37)] isolates, and two were suggestive of Methicillin-resistant Staphylococcus aureus (MRSA). Among the 37 isolates, 33 novel sequence types (ST) and two known STs (ST126 and ST746) were identified in MLST. The clonal complexes more frequently observed were: CC97 [78.38%; (29/37)], CC1 [8.11%; (3/37)] and CC5 [5.40%; (2/37)]. Minimum?spanning tree (MST) analysis according to data from municipalities, herds, and resistance patterns for all isolates did not show any clustering pattern. However, the MST comparing all Brazilian S. aureus isolates deposited in the PubMLST database and from this study depicted an association between the genotype and strain origin (clinical sample). Isolates from this study that belong to CC97 were close to database isolates from milk and dairy products, while those that belong to CC1 and CC5 were close to database isolates from human sources and the environment of dairy farms or industries. In conclusion, our results showed a high rate of resistance to penicillins and tetracyclines and great genetic diversity among the S. aureus isolates from bovine mastitis genotyped in the present study. RESUMO - Este estudo teve como objetivo avaliar a diversidade genética e a suscetibilidade a antimicrobianos de cepas de Staphylococcus aureus isoladas de vacas leiteiras em Minas Gerais, Brasil. Trinta e sete cepas provenientes de cinco municípios (oito rebanhos) foram genotipadas usando a técnica multi-locus sequence typing (MLST) e a suscetibilidade a 12 antimicrobianos foi avaliada pelo método de difusão em disco. Foram detectadas altas taxas de resistência para penicilina [75,68% (28/37)], ampicilina [70,27% (26/37)] e tetraciclina [70,27% (26/37)] entre os isolados. A multirresistência foi observada em sete [18,92% (7/37)] isolados e dois foram classificados como sugestivos de Staphylococcus aureus resistente à meticilina (MRSA). Entre os 37 isolados, 33 novos sequence types (ST) e dois STs conhecidos (ST126 e ST746) foram identificados pelo MLST. Os complexos clonais mais frequentemente observados foram: CC97 [78,38%; (29/37)], CC1 [8,11%; (3/37)] e CC5 [5,40%; (2/37)]. Foi construída uma minimum?spanning tree (MSTs) com todos isolados estudados e esta não mostrou padrão de agrupamento quando comparada com dados epidemiológicos como municípios e rebanho, do qual foi isolado, e perfis de resistência a antimicrobianos. Uma segunda MST foi construída comparando os isolados deste estudo e todas as cepas de S. aureus depositadas no banco de dados PubMLST provenientes do Brasil, que mostrou associação entre o genótipo, STs e a origem da cepa. Foi possível observar entre os isolados do estudo que, aqueles que pertenciam ao CC97, eram geneticamente mais próximos das cepas depositadas no PubMLST isoladas de leite e de produtos lácteos, enquanto aqueles que pertenciam aos CC1 e CC5 estavam mais próximos a cepas isoladas de humanos ou do ambiente de fazendas e indústrias de laticínios. Em conclusão, nossos resultados mostraram um alto índice de resistência às penicilinas e tetraciclinas e grande diversidade genética entre as cepas de S. aureus isoladas de casos de mastite bovina.
650 $aDairy industry
650 $aBovino
650 $aDoença Animal
650 $aEstafilococo
650 $aGado Leiteiro
650 $aVaca Leiteira
650 $aZoonose
653 $aMastite
653 $aMLST
653 $aMultidrug resistance
653 $aMultirresistência
653 $aSetor lácteo
653 $aStaphylococci
653 $aZoonosis
700 1 $aDORNELES, E. M. S.
700 1 $aHEINEMANN, M. B.
700 1 $aBRITO, M. A. V. P. e
700 1 $aGUIMARÃES, A. S.
773 $tCiência Rural, Santa Maria$gv. 53, n. 3, e20210643, 2023.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
03/04/2020 |
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Data da última atualização: |
20/07/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
JESUS, I. R. D. de; SILVA, P. C.; PEREIRA, M. B. C.; CARDOSO, M. S. L. D.; MACEDO, J. R. de; NASCIMENTO, P. P. |
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Afiliação: |
IGOR ROSA DIAS DE JESUS, CNPS; PAULA CRISTINA SILVA BASTOS, CNPS; MICHELE BELAS COUTINHO PEREIRA, CNPS; MELISSA SILVA LEME DALARME CARDOSO, CNPS; JOSE RONALDO DE MACEDO, CNPS; PETULA PONCIANO NASCIMENTO, CNPS. |
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Título: |
Work issues on Tomatec: tomato sustainable production system. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
In: INTERNATIONAL SYMPOSIUM ON AGRICULTURAL TECHNOLOGY ADOPTION, 2020, Campo Grande, MS. Studies, methods and experiences: abstracts. Campo Grande, MS: Embrapa Gado de Corte, 2020. p. 121-122. (Embrapa Gado de Corte. Documentos, 279). |
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Idioma: |
Inglês |
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Conteúdo: |
Tomatec is a tomato sustainable production system. It encompasses a set of good practices on the crop. The main practices are: irrigation through waterdropping, fertilization within irrigation (ferti-irrigation), integrated pest control (which heavily reduces the use of pesticides), good soil handling with no-tillage cropping and use of contours for planting, use of tiny wires for supporting tomato trees (instead of bamboo logs) and the enveloping of the fruits with paper bags. Enveloping remains as one of the most important practices due to two factors: first, it is visible. Tomatec crops are easily recognized by people because of the white paper bags that involves all bunches, turning all plantation into white. The other reason is because enveloping is a very laborious process, and the key for having fruits free of pesticide residues. In order to assess the impacts of Tomatec, using the Ambitec method, we have conducted four interviews with Tomatec producers in the State of Rio de Janeiro, Brazil. These interviews happened on 2019 September and took place in four municipalities: Tanguá, Nova Friburgo, Teresópolis and São Sebastião do Alto. Producers mention that farmworkers use individual protection equipment during their works. On field, we have seen hats and jackets. They mentioned that farmworkers also use masks for pesticides aspersion. Concerning to labour time, we have observed two main ways of contracting. Smaller producers usually work themselves in the fields. On harvest time, they contract many farmworkers, which are payed by numbers of day working. Bigger ones contract farmworkers by month and sign their workcard, paying the wages as well as the labour benefits, as disposed by Brazilian law. Another interesting finding about work on Tomatec is the genre division. All producers mentioned that pesticide sprinkle is exclusive to men, and enveloping is exclusive to women. It is not defined by rural producers: the farmworkers actually ask for that. Producers reported that farmworkers realize that sprinkling is a more painful and dangerous activity, with potential harms to fertility. Although Tomatec is recognised by strongly reducing the total amount of chemical products on the crop, men still want to save women from that kind of activity. On the other hand, enveloping is considered by farmworkers and producers a more delicate activity. It demands agility and precision to put on the paper bags on tomato flowers and to clip them, making the tomato fruits growing up inside the bags without receiving any drop of pesticide. Men are considered slower and less efficient than women to carry on this activity. So, analysing work issues on Tomatec we can see that the adoption of this production system have established many different strategies to deal with safety, law and genre issues. MenosTomatec is a tomato sustainable production system. It encompasses a set of good practices on the crop. The main practices are: irrigation through waterdropping, fertilization within irrigation (ferti-irrigation), integrated pest control (which heavily reduces the use of pesticides), good soil handling with no-tillage cropping and use of contours for planting, use of tiny wires for supporting tomato trees (instead of bamboo logs) and the enveloping of the fruits with paper bags. Enveloping remains as one of the most important practices due to two factors: first, it is visible. Tomatec crops are easily recognized by people because of the white paper bags that involves all bunches, turning all plantation into white. The other reason is because enveloping is a very laborious process, and the key for having fruits free of pesticide residues. In order to assess the impacts of Tomatec, using the Ambitec method, we have conducted four interviews with Tomatec producers in the State of Rio de Janeiro, Brazil. These interviews happened on 2019 September and took place in four municipalities: Tanguá, Nova Friburgo, Teresópolis and São Sebastião do Alto. Producers mention that farmworkers use individual protection equipment during their works. On field, we have seen hats and jackets. They mentioned that farmworkers also use masks for pesticides aspersion. Concerning to labour time, we have observed two main ways of contracting. Smaller producers usually work themselves in the fields. On ... Mostrar Tudo |
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Thesagro: |
Agricultura Sustentável; Sistema de Produção; Tomate. |
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Thesaurus NAL: |
Sustainable agriculture; Sustainable technology; Tomatoes. |
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Categoria do assunto: |
A Sistemas de Cultivo |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/212165/1/Work-issues-on-Tomatec-2020.pdf
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Marc: |
LEADER 03743nam a2200241 a 4500
001 2121546
005 2020-07-20
008 2020 bl uuuu u00u1 u #d
100 1 $aJESUS, I. R. D. de
245 $aWork issues on Tomatec$btomato sustainable production system.$h[electronic resource]
260 $aIn: INTERNATIONAL SYMPOSIUM ON AGRICULTURAL TECHNOLOGY ADOPTION, 2020, Campo Grande, MS. Studies, methods and experiences: abstracts. Campo Grande, MS: Embrapa Gado de Corte, 2020. p. 121-122. (Embrapa Gado de Corte. Documentos, 279).$c2020
520 $aTomatec is a tomato sustainable production system. It encompasses a set of good practices on the crop. The main practices are: irrigation through waterdropping, fertilization within irrigation (ferti-irrigation), integrated pest control (which heavily reduces the use of pesticides), good soil handling with no-tillage cropping and use of contours for planting, use of tiny wires for supporting tomato trees (instead of bamboo logs) and the enveloping of the fruits with paper bags. Enveloping remains as one of the most important practices due to two factors: first, it is visible. Tomatec crops are easily recognized by people because of the white paper bags that involves all bunches, turning all plantation into white. The other reason is because enveloping is a very laborious process, and the key for having fruits free of pesticide residues. In order to assess the impacts of Tomatec, using the Ambitec method, we have conducted four interviews with Tomatec producers in the State of Rio de Janeiro, Brazil. These interviews happened on 2019 September and took place in four municipalities: Tanguá, Nova Friburgo, Teresópolis and São Sebastião do Alto. Producers mention that farmworkers use individual protection equipment during their works. On field, we have seen hats and jackets. They mentioned that farmworkers also use masks for pesticides aspersion. Concerning to labour time, we have observed two main ways of contracting. Smaller producers usually work themselves in the fields. On harvest time, they contract many farmworkers, which are payed by numbers of day working. Bigger ones contract farmworkers by month and sign their workcard, paying the wages as well as the labour benefits, as disposed by Brazilian law. Another interesting finding about work on Tomatec is the genre division. All producers mentioned that pesticide sprinkle is exclusive to men, and enveloping is exclusive to women. It is not defined by rural producers: the farmworkers actually ask for that. Producers reported that farmworkers realize that sprinkling is a more painful and dangerous activity, with potential harms to fertility. Although Tomatec is recognised by strongly reducing the total amount of chemical products on the crop, men still want to save women from that kind of activity. On the other hand, enveloping is considered by farmworkers and producers a more delicate activity. It demands agility and precision to put on the paper bags on tomato flowers and to clip them, making the tomato fruits growing up inside the bags without receiving any drop of pesticide. Men are considered slower and less efficient than women to carry on this activity. So, analysing work issues on Tomatec we can see that the adoption of this production system have established many different strategies to deal with safety, law and genre issues.
650 $aSustainable agriculture
650 $aSustainable technology
650 $aTomatoes
650 $aAgricultura Sustentável
650 $aSistema de Produção
650 $aTomate
700 1 $aSILVA, P. C.
700 1 $aPEREIRA, M. B. C.
700 1 $aCARDOSO, M. S. L. D.
700 1 $aMACEDO, J. R. de
700 1 $aNASCIMENTO, P. P.
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